163,421 results

1. pLSSmOrange-C1

   Plasmid

   #37131

   Depositor

   Vladislav Verkhusha

   Article

   Shcherbakova et al J Am Chem Soc. 2012 Apr 24.

   Insert

   LSSmOrange
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   A44V/F84L/W145M/I165D/M167L/G202D compared to mOr…

   Promoter

   CMV

   Available since

   June 5, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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2. DNA repair library with 1,573 sgRNAs

   Pooled Library

   #177663

   Purpose

   CRISPR-interference (CRISPRi) library of 1,573 sgRNAs targeting 476 genes encoding factors involved in DNA repair and associated processes

   Depositor

   Brittany Adamson

   Article

   Hussmann et al Cell. 2021 Oct 28;184(22):5653-5669.e25.

   Species

   Homo sapiens

   Use

   CRISPR

   Available since

   July 11, 2023

   Availability

   Academic Institutions and Nonprofits only

3. pLentiCas9-GFP

   Plasmid

   #78546

   Purpose

   Fusion protein Cas9+GFP

   Depositor

   Rory Johnson, Roderic Guigo

   Article

   Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

   Insert

   GFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Fusion protein with Cas9

   Available since

   Sept. 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. PB-CA-rtTA Adv

   Plasmid

   #20910

   Purpose

   piggyBac vector for expression of reverse tetracycline transactivator from a CAG constitutive promoter

   Depositor

   Andras Nagy

   Article

   Woltjen et al Nature. 2009 Apr 9;458(7239):766-70. doi

   Insert

   rtTA-ON-Adv
   

   Use

   piggybac

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 23, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHR-PD-1 (Y223F, Y248F)-mGFP

   Plasmid

   #180790

   Purpose

   Expression of human PD-1 (Y223F, Y248F) fused to mEGFP

   Depositor

   Enfu Hui

   Article

   Xu et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

   Insert

   PD-1 (Y223F, Y248F) (PDCD1 Human)
   

   Use

   Lentiviral

   Tags

   mEGFP

   Expression

   Mutation

   PD-1 (Y223F, Y248F)

   Promoter

   SFFV

   Available since

   March 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3-Lyn-ICUE3

   Plasmid

   #61623

   Purpose

   Plasma membrane-targeted FRET biosensor for monitoring cAMP

   Depositor

   Jin Zhang

   Article

   DiPilato et al Mol Biosyst. 2009 Aug;5(8):832-7. doi: 1

   Insert

   Lyn-ICUE3 (RAPGEF3 Human)
   

   Use

   Tags

   ECFP, N-terminal targeting sequence from Lyn kina…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEGFP-Rab21-Q76L (CA)

   Plasmid

   #83422

   Purpose

   Mouse Rab21 mutant that has defects in GTP hydrolysis (GTP-locked, constitutively active). Generated from pEGFP-Rab21 by site-directed mutagenesis.

   Depositor

   Johanna Ivaska

   Article

   Pellinen et al J Cell Biol. 2006 Jun 5;173(5):767-80.

   Insert

   Rab21 (Rab21 Mouse)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Residue 76 from Q to L (Q76L: CAG -> CTG)

   Promoter

   CMV

   Available since

   Oct. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pREDawn-AmpR-MCS

   Plasmid

   #188978

   Purpose

   For red light-induced bacterial expression of gene of interest (AmpR)

   Depositor

   Heikki Takala

   Article

   Multamaki et al ACS Synth Biol. 2022 Aug 23. doi: 10.102

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBabe_Hyg_cOVA_T2A_mStrawberry

   Plasmid

   #161737

   Purpose

   Expression of cOVA and mStrawberry

   Depositor

   Alec Kimmelman

   Article

   Yamamoto et al Nature. 2020 May;581(7806):100-105. doi:

   Insert

   cytoplasmic ovalbumin
   

   Use

   Retroviral

   Tags

   mStrawberry

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFA6a-link-ymNeongreen-URA3

    Plasmid

    #168059

    Purpose

    Replaces Addgene Plasmid 125703. Yeast tagging vector to create a gene fusion with yeast optimized mNeongreen with CaUra3 selection; Contains the linker region at the 5’ end of the FP

    Depositor

    Bas Teusink

    Article

    Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

    Insert

    ymNeonGreen
    

    Use

    Tags

    ymNeonGreen

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBUE411

    Plasmid

    #62200

    Purpose

    CRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (OsU3 promoter), Bar resistance

    Depositor

    Qi-Jun Chen

    Article

    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

    Inserts

    gRNA scaffold
    zCas9
    

    Use

    Plant binary vector

    Tags

    3x FLAG and NLS

    Expression

    Plant

    Mutation

    maize codon optimized

    Promoter

    OsU3 and Ubi

    Available since

    April 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAVU6+27-F30-2xdBroccoli

    Plasmid

    #66842

    Purpose

    Expresses two dimeric Broccoli aptamers in the F30 scaffold in mammalian cells

    Depositor

    Samie Jaffrey

    Article

    Filonov et al Chem Biol. 2015 May 21;22(5):649-60. doi

    Insert

    Two dimeric Broccoli aptamers in the F30 scaffold
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    June 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV5-Smad7-HA

    Plasmid

    #11733

    Depositor

    Jeff Wrana

    Article

    Hayashi et al Cell. 1997 Jun 27. 89(7):1165-73.

    Insert

    SMAD7 (SMAD7 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 12, 2006

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pLVX-Puro-TDP-43-WT

    Plasmid

    #133753

    Purpose

    Wild type human TDP-43 in pLVX-Puro lentiviral vector

    Depositor

    Shawn Ferguson

    Article

    Roczniak-Ferguson et al Life Sci Alliance. 2019 Sep 16;2(5). pii

    Insert

    TDP-43 (TARDBP Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTF-lacZ-rfp

    Plasmid

    #153037

    Purpose

    CRISPR-Cas12a genome editing in E. coli. Contains a constitutively CRISPR array targeting the lacZ gene in E. coli genome and donor DNA to insert an constitutively expressed rfp reporter gene.

    Depositor

    Nigel Scrutton

    Article

    Jervis et al Microb Biotechnol. 2021 Mar 12. doi: 10.

    Insert

    CRISPR array
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSADdeltaG-F3

    Plasmid

    #32634

    Depositor

    Edward Callaway

    Article

    Osakada et al Neuron. 2011 Aug 25;71(4):617-31.

    Insert

    B19N, B19P, B19M and B19L
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    In frame insertion of Ser after aa10 in M (matrix…

    Promoter

    CMV

    Available since

    Oct. 26, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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17. Str-Ii_VSVG-SBP-mCherry

    Plasmid

    #65301

    Purpose

    synchronize trafficking of VSVG from the ER (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    VSV-G
    

    Use

    Tags

    Streptavidin Binding Protein (SBP) and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pNS35 (multiAsCas12a piggyBac)

    Plasmid

    #217336

    Purpose

    piggyBac expression of multiAsCas12a

    Depositor

    Luke Gilbert

    Article

    Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

    Insert

    multiAsCas12a
    

    Use

    CRISPR

    Tags

    6xMycNLS and HA-SV40NLS-P2A-TagBFP2

    Expression

    Mammalian

    Mutation

    R1226A/E174R/S542R/K548R

    Promoter

    CAG

    Available since

    March 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMV-pro-Il1b-C-Flag

    Plasmid

    #75131

    Purpose

    Expresses C-terminal flag-tagged pro-IL-1β in mammalian cells

    Depositor

    Bruce Beutler

    Article

    Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:

    Insert

    interleukin 1 beta (Il1b Mouse)
    

    Use

    Tags

    3XFLAG

    Expression

    Mammalian

    Mutation

    (please see depositor comments below)

    Promoter

    CMV

    Available since

    Sept. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3-HA-PHLPP2

    Plasmid

    #22403

    Depositor

    Alexandra Newton

    Article

    Brognard et al Mol Cell. 2007 Mar 23. 25(6):917-31.

    Insert

    PHLPP2 (PHLPP2 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 29, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pACAGW-ChR2-Venus-AAV (AAV1)

    Viral Prep

    #20071-AAV1

    Purpose

    Ready-to-use AAV1 particles produced from pACAGW-ChR2-Venus-AAV (#20071). In addition to the viral particles, you will also receive purified pACAGW-ChR2-Venus-AAV plasmid DNA. CAG-driven, channelrhodopsin fused to Venus for optogenetic activation. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Karel Svoboda

    Article

    Leopoldo et al Nature. 2009 Jan 18.

    Promoter

    CAGG

    Tags

    Venus

    Available since

    Feb. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

22. MigR1-AE

    Plasmid

    #12431

    Depositor

    Dong-Er Zhang

    Article

    Yan et al Proc Natl Acad Sci U S A. 2004 Dec 7. 10

    Insert

    AML1-ETO fusion (RUNX1, RUNX1T1 Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 2, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pAAV-Tnnt2-GFP-LA

    Plasmid

    #206198

    Purpose

    Expresses GFP-tagged Lamin-A specifically in cardiomyocytes by the Tnnt2 promoter

    Depositor

    Yuxuan Guo

    Article

    Sun et al JACC Basic Transl Sci. 2024 Aug 21;9(11)

    Insert

    Lmna (LMNA Human)
    

    Use

    AAV

    Tags

    GFP

    Expression

    Mutation

    Promoter

    Tnnt2

    Available since

    Aug. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. σ2-mCherry

    Plasmid

    #186579

    Purpose

    Expression of adaptor protein 2 (AP2) sigma subunit AP2S1 tagged with mCherry

    Depositor

    Stephen Royle

    Article

    Kuey et al Elife. 2022 Jul 19;11. pii: 78929. doi:

    Insert

    AP2S1 (AP2S1 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. mKO3-TEAD4

    Plasmid

    #221217

    Purpose

    RFP fused TEAD4

    Depositor

    Xiaokun Shu

    Article

    Chung et al Proc Natl Acad Sci U S A. 2024 Feb 13;12

    Insert

    mKO3-TEAD4 (TEAD4 )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pYPQ142

    Plasmid

    #69294

    Purpose

    Golden Gate recipient and Gateway entry vector; assembly of 2 gRNAs

    Depositor

    Yiping Qi

    Article

    Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. Anti-SynCAM1 [L45/30R]

    Plasmid

    #177482

    Purpose

    Mammalian Expression Plasmid of anti-SynCAM1 (Mouse). Derived from hybridoma L45/30.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    anti-SynCAM1 (Mus musculus) recombinant mouse monoclonal antibody (Cadm1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Dual CMV

    Available since

    Nov. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pHAGE-FGFR4

    Plasmid

    #116743

    Purpose

    Lentiviral expression of FGFR4

    Depositor

    Gordon Mills, Kenneth Scott

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    FGFR4 (FGFR4 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pAAV.EF1a.ChR2-YFP.WPRE.hGH

    Plasmid

    #100056

    Purpose

    AAV expression of ChR2 fused to YFP driven byEF1a promoter for optogenetic activation

    Depositor

    Karl Deisseroth

    Insert

    ChR2
    

    Use

    AAV

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. AID* Kit

    Plasmid Kit

    #1000000120

    Purpose

    Expanded tool kit for the auxin-inducible degron (AID) system in yeast ( S. cerevisiae). Includes tags for easy detection by antibodies/microscopy and a set of selection markers.

    Depositor

    Helle Ulrich

    Available since

    Jan. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

31. pET30-HPF1-His-Sumo-Flag

    Plasmid

    #111577

    Purpose

    Expresses full length HPF1 in E. coli

    Depositor

    Thomas Muir

    Article

    Liszczak et al Nat Chem Biol. 2018 Jul 16. pii: 10.1038

    Insert

    HPF1 (HPF1 Human)
    

    Use

    Tags

    6xHis-Sumo-FLAG

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pENN.AAV.EF1a.eGFP.WPRE.rBG (AAV1)

    Viral Prep

    #105547-AAV1

    Purpose

    Ready-to-use AAV1 particles produced from pENN.AAV.EF1a.eGFP.WPRE.rBG (#105547). In addition to the viral particles, you will also receive purified pENN.AAV.EF1a.eGFP.WPRE.rBG plasmid DNA. EF1a-driven EGFP expression. These AAV preparations are suitable purity for injection into animals.

    Depositor

    James M. Wilson

    Promoter

    EF1a

    Tags

    EGFP

    Available since

    Sept. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

33. Anti-Calbindin [L109/39R]

    Plasmid

    #177454

    Purpose

    Mammalian Expression Plasmid of anti-Calbindin (Human). Derived from hybridoma L109/39.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    anti-Calbindin (Homo sapiens) recombinant mouse monoclonal antibody (CALB1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Dual CMV

    Available since

    Nov. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pMX_mTBP

    Plasmid

    #44333

    Depositor

    Marc Timmers

    Article

    Pijnappel et al Nature. 2013 Mar 28;495(7442):516-9. doi

    Insert

    TBP (Tbp Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    LTR

    Available since

    May 14, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. Nsp12-EGFP

    Plasmid

    #165116

    Purpose

    mammalian expression and localization

    Depositor

    Bruno Antonny

    Article

    Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

    Insert

    Nsp12 (ORF1ab codon optimized SARS-COV-2)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. wtBxb1

    Plasmid

    #222337

    Purpose

    Plasmid for expressing wild type Bxb1 in mammalian cells

    Depositor

    David Liu

    Article

    Pandey et al Nat Biomed Eng. 2024 Jun 10. doi: 10.103

    Insert

    Codon-optimized wild type Bxb1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. TFORF0123

    Plasmid

    #141528

    Purpose

    Lentiviral vector for overexpressing the OLIG2 transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    OLIG2 (OLIG2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. HA-FOXO3a TM

    Plasmid

    #1788

    Depositor

    Michael Greenberg

    Article

    Brunet et al Cell 1999 Mar 19;96(6):857-68.

    Insert

    FOXO3a (FOXO3 Human)
    

    Use

    Tags

    HA (YDVPDYASL)

    Expression

    Mammalian

    Mutation

    T32A, S253A, S315A (no longer detectably phosphor…

    Promoter

    Available since

    April 20, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pAF0432 Ef1a-Dn29-2a-EGFP

    Plasmid

    #193459

    Purpose

    Expression of Dn29 recombinase from Ef1a promoter

    Depositor

    Patrick Hsu

    Article

    Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

    Insert

    Dn29
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1a

    Available since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pAAV-CaMKIIa-HA-KORD-IRES-mCitrine

    Plasmid

    #65418

    Purpose

    AAV packaging vector for CamKIIa driven KOR DREADD expression

    Depositor

    Bryan Roth

    Article

    Vardy et al Neuron. 2015 Apr 29. pii: S0896-6273(15)

    Insert

    CaMKIIa-HA-KORD-IRES-mCitrine (OPRK1 Human)
    

    Use

    AAV

    Tags

    HA and mCitrine

    Expression

    Mutation

    Promoter

    CaMKIIa

    Available since

    June 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge