167,318 results

1. CL20_mEGFP_NPM1_ALK

   Plasmid

   #205868

   Purpose

   Express mEGFP-tagged fusion protein, NPM1_ALK from patient-derived sequence

   Depositor

   Richard Kriwacki

   Article

   Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

   Insert

   NPM1_ALK (NPM1, ALK Human)
   

   Tags

   mEGFP

   Expression

   Mammalian

   Available Since

   Nov. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. AAT-PB-CD2APtk

   Plasmid

   #86004

   Purpose

   homology donor for AAT locus with piggyBac-flanked DsRedEx_T2A_puroTK cassette

   Depositor

   Tobias Cantz

   Article

   Eggenschwiler et al Sci Rep. 2016 Dec 2;6:38198. doi: 10.103

   Insert

   DsRedEx_T2A_puro_dTK
   

   Use

   Piggybac exciseable

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Feb. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGES201

   Plasmid

   #190197

   Purpose

   For CRISPR-Cas9 mediated gene editing in soybean, soybean elongation factor 1A promoter (pM4)-Cas9, GmU6-sgRNA, Basta selection

   Depositor

   Yuefeng Guan

   Article

   Bai et al Plant Biotechnol J. 2020 Mar;18(3):721-7

   Insert

   spCas9
   

   Use

   CRISPR

   Tags

   3xFlag

   Expression

   Plant

   Mutation

   plant-codon optimized

   Promoter

   Glycine max elongation factor 1A(pM4)

   Available Since

   Sept. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. ER-M-TAG (pGS555)

   Plasmid

   #160040

   Purpose

   pLV-A4-UCOE-EF1a-MMGT1-mScarlet-3xHA-IRES-BLAS-2A-SNAP-WPRE-A2

   Depositor

   David Sabatini

   Insert

   MMGT1-mScarlet-3xHA
   

   Use

   Lentiviral

   Promoter

   EF1a

   Available Since

   March 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAG-GCaMP6s

   Plasmid

   #171156

   Purpose

   Encodes GCaMP6s

   Depositor

   Hillel Adesnik

   Article

   Sridharan et al Neuron. 2022 Jan 27. pii: S0896-6273(22)

   Insert

   GCaMP6s
   

   Expression

   Mammalian

   Available Since

   Aug. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcw57.1-RquA-FLAG

   Plasmid

   #229685

   Purpose

   Doxycycline-inducible expression of RquA-FLAG

   Depositor

   Jessica Spinelli

   Article

   Valeros et al Cell. 2025 Jan 10:S0092-8674(24)01420-X.

   Insert

   RquA-FLAG
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   TRE

   Available Since

   Feb. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLIX403_Capture1_APOBEC_HA_P2A_mRuby

   Plasmid

   #183901

   Purpose

   Inducible lentiviral expression, TRE-gateway-APOBEC-HA-P2A-mRuby; PGK-puro-2A-rtTA

   Depositor

   Eugene Yeo

   Article

   Brannan et al Nat Methods. 2021 May;18(5):507-519. doi

   Type

   Empty backbone

   Use

   Lentiviral; Gateway destination vector, doxycycli…

   Tags

   Capture1_APOBEC_HA_P2A_mRuby

   Expression

   Mammalian

   Promoter

   TRE promoter, Tet ON

   Available Since

   Oct. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET MBP mCherry LIC cloning vector (MBP-mCherry)

   Plasmid

   #29747

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Tags

   6xHis, AviTag (biotin), MBP, TEV cleavage site, a…

   Expression

   Bacterial

   Available Since

   Sept. 22, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3 HA Arf6

   Plasmid

   #10834

   Depositor

   Thomas Roberts

   Article

   Furman et al J Biol Chem. 2002 Mar 8. 277(10):7962-9.

   Insert

   Arf6 (ARF6 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Available Since

   Jan. 5, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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10. pT7-VP1SA11

    Plasmid

    #89162

    Purpose

    Express Rotavirus SA11 VP1 in mammalian cells

    Depositor

    Takeshi Kobayashi

    Article

    Kanai et al Proc Natl Acad Sci U S A. 2017 Jan 30. p

    Insert

    Rotavirus SA11 VP1
    

    Expression

    Mammalian

    Promoter

    T7 promotor

    Available Since

    April 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. amajLime chromoprotein

    Plasmid

    #117843

    Purpose

    BioBrick pSB1C3 plasmid that constitutively overexpresses amajLime chromoprotein in E. coli

    Depositor

    Anthony Forster

    Article

    Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

    Insert

    promoter, RBS, amajLime
    

    Use

    Synthetic Biology; Escherichia coli

    Mutation

    BioBrick sites removed

    Available Since

    Aug. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pN1-GCaMP6m-XC

    Plasmid

    #111543

    Purpose

    Improved genetic encoded calcium sensor based on GCaMP6m. Also called GCaMP-X

    Depositor

    Xiaodong Liu

    Article

    Yang et al Nat Commun. 2018 Apr 17;9(1):1504. doi:

    Insert

    GCaMP6m-XC
    

    Expression

    Mammalian

    Available Since

    June 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. opto-Cdc42

    Plasmid

    #174510

    Purpose

    Expresses opto-Cdc42 (mCherry-GGGSx2-Cdc42-GGGSx2-BcLOV4-GGGS-3xFLAG) in a pcDNA3.1 backbone.

    Depositor

    Brian Chow

    Article

    Berlew et al ACS Synth Biol. 2022 Jan 21;11(1):515-52

    Insert

    opto-Cdc42-mCherry
    

    Tags

    3xFLAG and mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pKLV2-EF1a-BsdCas9-W

    Plasmid

    #67978

    Purpose

    Lentiviral vector expressing Cas9 fused with the Blasticidin resistant gene at the N-terminus

    Depositor

    Kosuke Yusa

    Article

    Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

    Insert

    EF1a-Cas9 cassette, WPRE
    

    Use

    CRISPR and Lentiviral

    Tags

    Cas9 fused with Flag-tag and a NLS at the N termi…

    Promoter

    EF1a

    Available Since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. PB-TRE-dCas9-Zim3-MeCP2

    Plasmid

    #220143

    Purpose

    doxycycline-inducible dCas9-Zim3-MeCP2 expression for Epigenetic repression

    Depositor

    Frank Buchholz

    Article

    Ding et al Life Sci Alliance. 2022 Mar 14;5(6). pii

    Insert

    dCas9-Zim3-MeCP2
    

    Use

    CRISPR and Synthetic Biology; Piggybac

    Expression

    Mammalian

    Promoter

    Tre-3G

    Available Since

    June 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. 764 pME nls-mApple

    Plasmid

    #195962

    Purpose

    Gateway pME fluorophore

    Depositor

    Christian Mosimann, Kristen Kwan

    Article

    Kemmler et al Development. 2023 Mar 28:dev.201531. doi

    Insert

    mApple nuclear
    

    Use

    Other

    Available Since

    Feb. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMBIS

    Plasmid

    #17817

    Depositor

    Jay Keasling

    Article

    Martin et al Nat Biotechnol. 2003 Jul . 21(7):796-802

    Insert

    mevalonate kinase, phosphomevalonate kinase, mevalonate pyrophosphate decarboxylatse, IPP isomerase, FPP synthase
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    July 27, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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18. pETM6-RgTALsyn

    Plasmid

    #100947

    Purpose

    Expression of Tyrosine Ammonia Lyase from inducible T7 promoter

    Depositor

    Mattheos Koffas

    Article

    Jones et al MBio. 2017 Jun 6;8(3). pii: e00621-17. d

    Insert

    RgTALsyn
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Mutation

    Silent mutation to remove internal NdeI site on T…

    Promoter

    T7

    Available Since

    Sept. 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. Watermelon backbone

    Plasmid

    #155258

    Purpose

    Can be used to generate a Watermelon library

    Depositor

    Aviv Regev, Joan Brugge

    Article

    Oren et al Nature. 2021 Aug 11. pii: 10.1038/s41586

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Aug. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pUB6-HuMGF

    Plasmid

    #190782

    Purpose

    Expresses membrane-bound form of human SCF in mammalian cells

    Depositor

    Jakob Reiser

    Article

    Zhang et al Retrovirology. 2010 Jan 25;7:3. doi: 10.

    Insert

    human stem cell factor (KITLG Human)
    

    Expression

    Mammalian

    Promoter

    UbC

    Available Since

    Nov. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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21. pT7-NSP1SA11

    Plasmid

    #89168

    Purpose

    Express Rotavirus SA11 NSP1 in mammalian cells

    Depositor

    Takeshi Kobayashi

    Article

    Kanai et al Proc Natl Acad Sci U S A. 2017 Jan 30. p

    Insert

    Rotavirus SA11 NSP1
    

    Expression

    Mammalian

    Promoter

    T7 promotor

    Available Since

    April 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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22. pHTN_HaloTag_KIF5B

    Plasmid

    #213404

    Purpose

    Full-length human kinesin-1 with HaloTag at the N-terminus

    Depositor

    Jonas Ries

    Article

    Deguchi et al Science. 2023 Mar 10;379(6636):1010-1015

    Insert

    HaloTag-KIF5B (KIF5B Human)
    

    Tags

    HaloTag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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23. HSA1_His

    Plasmid

    #202763

    Purpose

    human serum albumin design HSA1, in pET29b with C-terminal 6His-tag

    Depositor

    Sarel Fleishman

    Article

    Khersonsky et al J Mol Biol. 2023 Sep 1;435(17):168191. d

    Insert

    HSA1
    

    Tags

    His tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Dec. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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24. pMP71-HLA-A0201-His

    Plasmid

    #108214

    Purpose

    HLA-A0201 his tagged

    Depositor

    Sébastien Walchli

    Article

    Lampen et al Immunohorizons. 2018 Jul 2;2(6):172-184.

    Insert

    major histocompatibility complex, class I, B (HLA-A Human)
    

    Use

    Retroviral

    Tags

    his

    Expression

    Mammalian

    Available Since

    Dec. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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25. pRN3P_T3_ABE8e_IVT

    Plasmid

    #201676

    Purpose

    Plasmid to be used as DNA template for in vitro RNA transcription of the ABE8e base editor (A to G) by T3 RNA polymerase. The plasmid contains optimised 5'UTR and 3'UTR to improve protein expression.

    Depositor

    Timo Otonkoski

    Article

    Jalil et al Am J Hum Genet. 2024 Apr 4;111(4):714-72

    Insert

    ecTadA(8e)-nSpCas9
    

    Use

    CRISPR; Vector for in-vitro transcription

    Promoter

    T3 promoter

    Available Since

    April 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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26. pmCherry-C1 actin-3XNLS P2A mCherry

    Plasmid

    #58475

    Purpose

    Expresses nuclear-targeted human actin, with an mCherry expression reporter after a P2A protease cleavage site, on a CMV promoter

    Depositor

    Dyche Mullins

    Article

    Belin et al Elife. 2015 Aug 19;4. doi: 10.7554/eLife

    Inserts

    beta actin (ACTB Human)
    3XNLS
    P2A cleavage site
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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27. pcDNA3.1-Flag-PGC-1alpha1

    Plasmid

    #45501

    Depositor

    Jorge Ruas, Bruce Spiegelman

    Article

    Ruas et al Cell. 2012 Dec 7;151(6):1319-31. doi: 10

    Insert

    PGC-1alpha Isoform1 (Ppargc1a Mouse)
    

    Tags

    6xHis and Flag

    Expression

    Mammalian

    Mutation

    Q339K relative to NM_008904

    Available Since

    Aug. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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28. HA-Halotag-FRB-EX-nes_pLX304

    Plasmid

    #120913

    Purpose

    Lentiviral vector expressing HA-Halotag-FRB-EX-NES in mammalian cytosol

    Depositor

    Alice Ting

    Article

    Han et al ACS Chem Biol. 2019 Mar 8. doi: 10.1021/

    Insert

    HA-Halotag-FRB-EX-NES
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    March 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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29. pCDNA3.3 N-MYC TIP60

    Plasmid

    #213003

    Purpose

    expresses human TIP60

    Depositor

    Sheng-cai Lin

    Article

    Wu et al Cell Res. 2023 Nov;33(11):835-850. doi:

    Insert

    KAT5 (KAT5 Human)
    

    Tags

    MYC

    Expression

    Mammalian

    Promoter

    cmv

    Available Since

    Feb. 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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30. pcDNA3-mNeptune2.5

    Plasmid

    #51310

    Purpose

    Expresses far-red fluorescent protein mNeptune2.5 in mammalian cells

    Depositor

    Michael Lin

    Article

    Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

    Insert

    mNeptune2.5
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 8, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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