165,191 results

1. nesprin TSmod 25

   Plasmid

   #125555

   Purpose

   Nesprin-2G force sensor with cerulean-F25-venus TSmodule

   Depositor

   Daniel Conway

   Article

   Mayer et al Cell Mol Bioeng. 2019 Jul 11;12(4):289-3

   Insert

   mini-nesprin-2G (Syne2 Mouse)
   

   Use

   Tags

   cerulean, venus

   Expression

   Mammalian

   Mutation

   TSmod changed from mTFP to cerulean, flageliform …

   Promoter

   CMV

   Available since

   July 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pPET-PKR/PPase

   Plasmid

   #42934

   Depositor

   James Cole

   Article

   Lemaire et al J Mol Biol. 2005 Jan 7;345(1):81-90.

   Inserts

   protein kinase R (EIF2AK2 Human)
   phage lambda phosphatase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 12, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMDR1-1202

   Plasmid

   #37627

   Depositor

   Kathleen Scotto

   Article

   Hu et al J Biol Chem. 2000 Jan 28;275(4):2979-85.

   Insert

   MDR1 promoter (ABCB1 Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 2, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-hSyn-EGFP (AAV5 trial size)

   Viral Prep

   #50465-AAV5.T

   Purpose

   Ready-to-use AAV5 trial size particles produced from pAAV-hSyn-EGFP (#50465). In addition to the viral particles, you will also receive purified pAAV-hSyn-EGFP plasmid DNA. hSyn-driven EGFP expression. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Bryan Roth

   Promoter

   Syn

   Tags

   EGFP

   Available since

   June 12, 2019

   Availability

   Academic Institutions and Nonprofits only

5. IRE1 alpha-pcDNA3.EGFP

   Plasmid

   #13009

   Depositor

   Fumihiko Urano

   Article

   Lipson et al Cell Metab. 2006 Sep . 4(3):245-54.

   Insert

   Inositol Requiring Enzyme-1 (ERN1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 27, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLX401-INK4A

   Plasmid

   #121919

   Purpose

   Doxycycline-inducible expression of p16-INK4A product of CDKN2A

   Depositor

   William Hahn

   Article

   Howard et al Cancer Res. 2019 Feb 12. pii: 0008-5472.

   Insert

   p16-INK4A (CDKN2A Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE Tet ON

   Available since

   April 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLJM1 Flag Raptor

   Plasmid

   #26633

   Depositor

   David Sabatini

   Article

   Sancak et al Cell. 2010 Apr 16. 141(2):290-303.

   Insert

   raptor (RPTOR Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 22, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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8. VIPR2-Tango

   Plasmid

   #66521

   Purpose

   Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

   Depositor

   Bryan Roth

   Article

   Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

   Insert

   VIPR2 (VIPR2 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

9. pJOP-HTT-HR45Q

   Plasmid

   #92248

   Purpose

   HTT donor DNA with 45Q for homologous recombination-mediated gene modification

   Depositor

   Mahmoud Pouladi

   Article

   Ooi et al Cell Rep. 2019 Feb 26;26(9):2494-2508.e7

   Insert

   HTT donor DNA (HTT Human)
   

   Use

   Precisionx hr targeting vector

   Tags

   Expression

   Mutation

   Barcode of wobble bases to prevent TALEN cleaving…

   Promoter

   Available since

   Feb. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHR Grb2-TagBFP

    Plasmid

    #188631

    Purpose

    Human Grb2 C-terminally labeled with TagBFP

    Depositor

    Jared Toettcher

    Article

    Farahani et al eLife. 2023 May 22;12:e82863. doi: 10.75

    Insert

    GRB2 (GRB2 Human, Synthetic)
    

    Use

    Lentiviral

    Tags

    TagBFP

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    Aug. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. EC70197

    Plasmid

    #209458

    Purpose

    Cloning protospacers for Cas9 editing

    Depositor

    Wendy Harwood, Cristobal Uauy

    Article

    Lawrenson et al Research Square, 12 March 2024, PREPRINT

    Insert

    Cas9 sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    TaU3

    Available since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pscALPS- SIV-MAC251 vpx

    Plasmid

    #115810

    Purpose

    Encodes codon optimized 3xFLAG-VpxMAC251 and puromycin resistance protein

    Depositor

    Jeremy Luban

    Article

    Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

    Insert

    SIVMAC251 Vpx
    

    Use

    Lentiviral

    Tags

    3x-FLAG

    Expression

    Mutation

    Promoter

    SFFV

    Available since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJL 89

    Plasmid

    #98558

    Purpose

    Useful for making infectious clones of RNA virus cDNAs; Has 35S promoter and HDV Ribozyme sequences in T-DNA region of Agrobacterium vector.

    Depositor

    John Lindbo

    Article

    Lindbo et al Plant Physiol. 2007 Dec;145(4):1232-40.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Enh 35S

    Available since

    Aug. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. TFORF1874

    Plasmid

    #143329

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    BBX (BBX Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    July 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. TFORF2746

    Plasmid

    #144351

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    HELZ2 (PRIC285 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Aug. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMD2.G-K47Q/R354Q

    Plasmid

    #207323

    Purpose

    VSV-G mutant with abolished receptor binding, but maintained fusogenic capacity

    Depositor

    Feng Zhang

    Article

    Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

    Insert

    VSV-G (VSIVgp4 Vesicular Stomatitis Virus)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    K47Q/R354Q

    Promoter

    CMV promoter

    Available since

    Nov. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pU6-Sp-pegRNA-HEK3_CTT_ins

    Plasmid

    #132778

    Purpose

    S. pyogenes pegRNA for CTT insertion at the HEK3 site of human cells using prime editing

    Depositor

    David Liu

    Article

    Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586

    Insert

    HEK3_CTT_ins pegRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    See manuscript

    Promoter

    U6

    Available since

    Oct. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHR-tdMCP-YFP

    Plasmid

    #99151

    Purpose

    Expresses dimeric MS2-coat binding protein tagged with YFP, 2nd generation lentiviral vector

    Depositor

    Ron Vale

    Article

    Jain et al Nature. 2017 Jun 8;546(7657):243-247. do

    Insert

    Dimeric MS2-coat binding protein
    

    Use

    Lentiviral

    Tags

    eYFP

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    Aug. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA-dCas9-FLp300

    Plasmid

    #61356

    Purpose

    encodes S. pyogenes dCas9 with c-terminal fusion of FL human p300 (aa 2-2414) driven by CMV promoter

    Depositor

    Charles Gersbach

    Article

    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do

    Insert

    S.pyogenes dCas9 with c-terminal full length human p300 (aa 2-2414) (EP300 S. pyogenes, Human, Synthetic)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Flag, HA, and SV40 NLS

    Expression

    Mammalian

    Mutation

    D10A; H840A in S.pyogenes Cas9

    Promoter

    CMV

    Available since

    April 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3-TDsmURFP-IRES-HO-1

    Plasmid

    #80346

    Purpose

    Expresses Tandem Dimer smURFP and Heme Oxygenase-1 in mammalian cells.

    Depositor

    Erik Rodriguez, Roger Tsien

    Article

    Rodriguez et al Nat Methods. 2016 Aug 1. doi: 10.1038/nm

    Inserts

    Tandem Dimer smURFP
    HO-1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and IRES

    Available since

    Aug. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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21. pSEM318 - sgRNA mosTI unc-119 single-copy

    Plasmid

    #159822

    Purpose

    Single copy or array insertion by MosTI using split unc-119 selection

    Depositor

    Christian Froekjaer-Jensen

    Article

    El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

    Insert

    sgRNA mosTI unc-119 single-copy
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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22. pRVL-5

    Plasmid

    #104583

    Purpose

    Contains human IgG1 CH1/CH2/CH3 domains, for cloning of antibody VH domains using SapI restriction enzyme. Full Fc-mediated immune effector functions

    Depositor

    Daniel Christ

    Article

    Vazquez-Lombardi et al Methods Mol Biol. 2018;1827:313-334. doi

    Insert

    Human IgG1 CH1/CH2/CH3 (IGHG1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pcDNA3.1-SARS-Spike

    Plasmid

    #145031

    Purpose

    Express SARS-CoV spike protein with C9 tag at C-terminal in mammalian cells

    Depositor

    Fang Li

    Article

    Shang et al Nature. 2020 Mar 30. pii: 10.1038/s41586

    Insert

    SARS-CoV Spike
    

    Use

    Tags

    C9 and CD5 signal peptide

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 16, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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24. pLX307-CRBN-3F-BirA

    Plasmid

    #220146

    Purpose

    Expresses CRBN-BirA for CRBN substrate identification by E-STUB

    Depositor

    William Sellers

    Article

    Huang et al Nat Chem Biol. 2024 Mar 21. doi: 10.1038

    Insert

    Cereblon (CRBN Human)
    

    Use

    Lentiviral

    Tags

    3F-BirA

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available since

    May 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pMCP-Luc (MCP-1 promoter in pGL3-basic)

    Plasmid

    #40324

    Depositor

    Alexander Dent

    Article

    Toney et al Nat Immunol. 2000 Sep;1(3):214-20.

    Insert

    MCP-1 promoter (Ccl2 Mouse)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    contains MCP-1 promoter region through the nucleo…

    Promoter

    MCP-1

    Available since

    Sept. 28, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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26. pLVX-Tight-Puro-KrasG12D2B

    Plasmid

    #139786

    Purpose

    Lentiviral tet-inducible expression of KrasG12D (mouse)

    Depositor

    Joan Massague

    Article

    Su et al Nature. 2020 Jan;577(7791):566-571. doi:

    Insert

    KrasG12D (Kras Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    G12D

    Promoter

    Available since

    March 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. RBD-Fc

    Plasmid

    #164221

    Purpose

    Expresses soluble , dimeric Fc-His fusion protein for the original SARS-CoV2 Spike RBD region, along with dTomato reporter

    Depositor

    Sriram Neelamegham

    Article

    Yang et al Elife. 2020 Oct 26;9. pii: 61552. doi: 1

    Insert

    Spike RBD (S SARS-CoV)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. Green cGull

    Plasmid

    #86867

    Purpose

    Green fluorescent probe for cGMP

    Depositor

    Tetsuya Kitaguchi

    Article

    Matsuda et al ACS Sens. 2017 Jan 27;2(1):46-51. doi: 1

    Insert

    Green cGull
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pAAV-CBh-DIO-EGFP

    Plasmid

    #87168

    Purpose

    Expression of eGFP in the presence of Cre

    Depositor

    Jimok Kim

    Article

    Li et al Neuroscience. 2017 Sep 6. pii: S0306-452

    Insert

    EGFP
    

    Use

    AAV

    Tags

    None

    Expression

    Mutation

    Promoter

    Available since

    Sept. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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30. MSCV-(mcs) human CSF1 full length-IRES-Puro

    Plasmid

    #86797

    Purpose

    retroviral expression of CSF-1

    Depositor

    Charles Sherr, Martine Roussel

    Article

    Roussel et al Nature. 1987 Feb 5-11;325(6104):549-52.

    Insert

    CSF-1 (CSF1 Human)
    

    Use

    Retroviral

    Tags

    IRES-Puro

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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