337,922 results

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pRK5-HA-UFM1

Plasmid

#134639

Purpose

expression of HA-tagged human UFM1 wildtype

Depositor

Yihong Ye

Article

Wang et al Cell Res. 2020 Jan;30(1):5-20. doi: 10.1

Insert

UFM1 (UFM1 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 19, 2019

Availability

Academic Institutions and Nonprofits only

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Lenti-dCas9-TurboID-blast

Plasmid

#222599

Purpose

Express fussed dCas9-TurboID for proximity labeling

Depositor

Yarui Diao

Article

Sun et al Nat Genet. 2023 Aug;55(8):1324-1335. doi

Insert

dCas9-TurboID

Use

Lentiviral

Promoter

EF-1α

Available Since

July 25, 2024

Availability

Academic Institutions and Nonprofits only

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Lenti-dCas9-TurboID-blast

Plasmid

#222599

Purpose

Express fussed dCas9-TurboID for proximity labeling

Depositor

Yarui Diao

Article

Sun et al Nat Genet. 2023 Aug;55(8):1324-1335. doi

Insert

dCas9-TurboID

Use

Lentiviral

Promoter

EF-1α

Available Since

July 25, 2024

Availability

Academic Institutions and Nonprofits only

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p667-UBC-CDK12-V5-miniTurbo_IDG-K

Plasmid

#135243

Purpose

Gateway destination clone of CDK12 (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomics

Depositor

Ben Major

Insert

CDK12-V5-miniTurbo (CDK12 Human)

Use

Lentiviral; Gateway destination

Tags

V5-miniTurbo

Expression

Mammalian

Promoter

Ubiquitin

Available Since

April 15, 2020

Availability

Academic Institutions and Nonprofits only

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p667-UBC-CDK12-V5-miniTurbo_IDG-K

Plasmid

#135243

Purpose

Gateway destination clone of CDK12 (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomics

Depositor

Ben Major

Insert

CDK12-V5-miniTurbo (CDK12 Human)

Use

Lentiviral; Gateway destination

Tags

V5-miniTurbo

Expression

Mammalian

Promoter

Ubiquitin

Available Since

April 15, 2020

Availability

Academic Institutions and Nonprofits only

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PLKO.1-Scrambled

Plasmid

#136035

Purpose

Scrambled shRNA (negative control) inserted into the PLKO.1 plasmid (CCTAAGGTTAAGTCGCCCTCG)

Depositor

Anthony Leung

Article

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Insert

None (Scrambled)

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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PLKO.1-Scrambled

Plasmid

#136035

Purpose

Scrambled shRNA (negative control) inserted into the PLKO.1 plasmid (CCTAAGGTTAAGTCGCCCTCG)

Depositor

Anthony Leung

Article

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Insert

None (Scrambled)

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pEBTetD-SNAP-Centrin2

Plasmid

#136785

Purpose

Mammalian expression of the centrosomal protein Centrin2 N-terminally fused to SNAP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

SNAP-Centrin2 (CETN2 Synthetic, Human)

Tags

FLAG-tag / His-tag

Expression

Mammalian

Promoter

CMV-TetO2

Available Since

March 27, 2020

Availability

Academic Institutions and Nonprofits only

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pEBTetD-SNAP-Centrin2

Plasmid

#136785

Purpose

Mammalian expression of the centrosomal protein Centrin2 N-terminally fused to SNAP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

SNAP-Centrin2 (CETN2 Synthetic, Human)

Tags

FLAG-tag / His-tag

Expression

Mammalian

Promoter

CMV-TetO2

Available Since

March 27, 2020

Availability

Academic Institutions and Nonprofits only

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pAM-AAV-hSncg-EGFP

Plasmid

#153198

Purpose

Human gamma-synuclein (hSncg) promoter-mediates gene expression in retinal neurons with fluorescent reporter

Depositor

Yang Hu

Article

Wang et al J Neurosci. 2020 Apr 9. pii: JNEUROSCI.0

Type

Empty backbone

Use

AAV

Expression

Mammalian

Available Since

July 10, 2020

Availability

Academic Institutions and Nonprofits only

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pAM-AAV-hSncg-EGFP

Plasmid

#153198

Purpose

Human gamma-synuclein (hSncg) promoter-mediates gene expression in retinal neurons with fluorescent reporter

Depositor

Yang Hu

Article

Wang et al J Neurosci. 2020 Apr 9. pii: JNEUROSCI.0

Type

Empty backbone

Use

AAV

Expression

Mammalian

Available Since

July 10, 2020

Availability

Academic Institutions and Nonprofits only

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pEBTet-SNAP-HsSAS6

Plasmid

#136789

Purpose

Mammalian expression of the centrosomal protein SAS6 N-terminally fused to SNAP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

SNAP-SAS6 (SASS6 Synthetic, Human)

Tags

FLAG-tag / His-tag

Expression

Mammalian

Promoter

CMV-TetO2

Available Since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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pEBTet-SNAP-HsSAS6

Plasmid

#136789

Purpose

Mammalian expression of the centrosomal protein SAS6 N-terminally fused to SNAP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

SNAP-SAS6 (SASS6 Synthetic, Human)

Tags

FLAG-tag / His-tag

Expression

Mammalian

Promoter

CMV-TetO2

Available Since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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pEBTet-SNAP-Centrin2

Plasmid

#136784

Purpose

Mammalian expression of the centrosomal protein Centrin2 N-terminally fused to SNAP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

SNAP-Centrin2 (CETN2 Synthetic, Human)

Tags

FLAG-tag / His-tag

Expression

Mammalian

Promoter

CMV-TetO2

Available Since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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pEBTet-SNAP-Centrin2

Plasmid

#136784

Purpose

Mammalian expression of the centrosomal protein Centrin2 N-terminally fused to SNAP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

SNAP-Centrin2 (CETN2 Synthetic, Human)

Tags

FLAG-tag / His-tag

Expression

Mammalian

Promoter

CMV-TetO2

Available Since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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pTCF156 HLA-A*02:01

Plasmid

#180456

Purpose

E. coli expression of soluble MHC protein

Depositor

John Altman

Insert

HLA-A*02:01

Tags

BSP41

Expression

Bacterial

Mutation

Replacement of transmembrane domain with enzymati…

Available Since

March 3, 2022

Availability

Academic Institutions and Nonprofits only

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pTCF156 HLA-A*02:01

Plasmid

#180456

Purpose

E. coli expression of soluble MHC protein

Depositor

John Altman

Insert

HLA-A*02:01

Tags

BSP41

Expression

Bacterial

Mutation

Replacement of transmembrane domain with enzymati…

Available Since

March 3, 2022

Availability

Academic Institutions and Nonprofits only

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pHAGE_puro

Plasmid

#118692

Purpose

Empty vector to express overexpression cDNA

Depositor

Christopher Vakoc

Article

Lu et al Cancer Cell. 2018 Dec 10;34(6):970-981.e

Type

Empty backbone

Use

Lentiviral

Promoter

CMV

Available Since

Dec. 7, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pHAGE_puro

Plasmid

#118692

Purpose

Empty vector to express overexpression cDNA

Depositor

Christopher Vakoc

Article

Lu et al Cancer Cell. 2018 Dec 10;34(6):970-981.e

Type

Empty backbone

Use

Lentiviral

Promoter

CMV

Available Since

Dec. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pCS-memb-mCherry

Plasmid

#53750

Purpose

Contains mCherry fused to a 2x membrane localization sequence in the pCS2+ vector.

Depositor

Sean Megason

Article

Megason et al Methods Mol Biol. 2009;546:317-32. doi:

Insert

2x membrane linker

Tags

mCherry

Expression

Mammalian

Promoter

simian CMV IE94

Available Since

June 10, 2014

Availability

Academic Institutions and Nonprofits only

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pCS-memb-mCherry

Plasmid

#53750

Purpose

Contains mCherry fused to a 2x membrane localization sequence in the pCS2+ vector.

Depositor

Sean Megason

Article

Megason et al Methods Mol Biol. 2009;546:317-32. doi:

Insert

2x membrane linker

Tags

mCherry

Expression

Mammalian

Promoter

simian CMV IE94

Available Since

June 10, 2014

Availability

Academic Institutions and Nonprofits only

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pSN16

Plasmid

#100103

Purpose

expresses a chimeric OCT4-activator fusion

Depositor

Mark Ptashne

Article

Narayan et al Cell Rep. 2017 Aug 15;20(7):1585-1596. d

Insert

vexGFP-P2A-OCT4 fused to a 'strong' VP16 domain (POU5F1 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pSN16

Plasmid

#100103

Purpose

expresses a chimeric OCT4-activator fusion

Depositor

Mark Ptashne

Article

Narayan et al Cell Rep. 2017 Aug 15;20(7):1585-1596. d

Insert

vexGFP-P2A-OCT4 fused to a 'strong' VP16 domain (POU5F1 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pLV x EF1a_EKAREN5-ires-puro

Plasmid

#167821

Purpose

Optimized EKAREV FRET biosensor sensor for ERK

Depositor

Hugo Snippert

Article

Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

Insert

EKAREN5

Use

Lentiviral

Tags

nls localization motif

Expression

Mammalian

Mutation

K424P, K426W

Promoter

EF1a

Available Since

Aug. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pLV x EF1a_EKAREN5-ires-puro

Plasmid

#167821

Purpose

Optimized EKAREV FRET biosensor sensor for ERK

Depositor

Hugo Snippert

Article

Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

Insert

EKAREN5

Use

Lentiviral

Tags

nls localization motif

Expression

Mammalian

Mutation

K424P, K426W

Promoter

EF1a

Available Since

Aug. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-CD14

Plasmid

#13645

Depositor

Doug Golenbock

Insert

CD14 (CD14 Human)

Expression

Mammalian

Available Since

June 12, 2007

Availability

Academic Institutions and Nonprofits only

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pcDNA3-CD14

Plasmid

#13645

Depositor

Doug Golenbock

Insert

CD14 (CD14 Human)

Expression

Mammalian

Available Since

June 12, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pBABE-hygro CDK4 R24C

Plasmid

#11254

Depositor

Bob Weinberg

Article

Hahn et al Mol Cell Biol 2002 Apr;22(7):2111-23.

Insert

CDK4 (CDK4 Human)

Use

Retroviral

Expression

Mammalian

Mutation

R24C

Available Since

Feb. 14, 2006

Availability

Academic Institutions and Nonprofits only

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pBABE-hygro CDK4 R24C

Plasmid

#11254

Depositor

Bob Weinberg

Article

Hahn et al Mol Cell Biol 2002 Apr;22(7):2111-23.

Insert

CDK4 (CDK4 Human)

Use

Retroviral

Expression

Mammalian

Mutation

R24C

Available Since

Feb. 14, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV[Exp]-Hygro-CMV-FUCCIplex

Plasmid

#240221

Purpose

Multiplexable FUCCI sensor (FUCCIplex). The CMV promoter drives the expression of the fluorescent sensor.

Depositor

Francesco Pasqualini

Article

Di Sante et al bioRxiv 2024.12.19.629259

Insert

FUCCIplex

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 11, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge