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We narrowed to 15,033 results for: pET

Showing: 1651 - 1700 of 15033 results

  2. pET28a hMDH1v3 (+TEV)

    Plasmid
    #204229
    Purpose
    Bacterial expression of human cytosolic malate dehydrogenase isoform 1 transcript variant 3 (hMDH1v3); Use in CURES (integration of research into undergraduate teaching labs).
    Depositor
    Malate dehydrogenase CUREs Community (MCC)
    Insert
    hMDH1v3 (MDH1 Human)
    Use
    Tags
    6X His
    Expression
    Bacterial
    Mutation
    Inserted a G after ATG to maintain kozak site
    Promoter
    Lac
    Available Since
    Nov. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    204229-plasmid-map-sequence-id-413334

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  3. pET21 MBPx-SpyCatcher

    Plasmid
    #72327
    Purpose
    Bacterial expression of tandem high affinity maltose binding protein domains linked to SpyCatcher, for solid-phase polyprotein synthesis.
    Depositor
    Mark Howarth
    Article
    Veggiani et al Proc Natl Acad Sci U S A. 2016 Jan 19. p
    Insert
    pET21 MBPx-SpyCatcher
    Use
    Tags
    N-terminal His6 tag
    Expression
    Bacterial
    Mutation
    MBP contains A312V, I317V mutations and 172, 173,…
    Promoter
    Available Since
    Feb. 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    72327-plasmid-map-sequence-id-434061

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  5. pET28-eIF4E-TwStrep

    Plasmid
    #208638
    Purpose
    for E. coli expression of C-ter Twin Strep tagged human eIF4E (ENST00000450253)
    Depositor
    Quanfu Ma
    Insert
    human eIF4E (ENST00000450253) (EIF4E Human)
    Use
    Tags
    6xHis and Twin Strep
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available Since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208638-plasmid-map-sequence-id-415918

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  8. pET-MBP-mSA2

    Plasmid
    #52319
    Purpose
    Solubly expresses monomeric streptavidin containing S25H mutation in E. coli as an MBP fusion. App Microbiol & Biotech 98, 6285-6295 (2014)
    Depositor
    Sheldon Park
    Article
    Demonte et al Appl Microbiol Biotechnol. 2014;98(14):6
    Insert
    monomeric streptavidin 2
    Use
    Tags
    FLAG, His, MBP, and TEV
    Expression
    Bacterial
    Mutation
    S25H mutation compared to streptavidin
    Promoter
    T7
    Available Since
    July 15, 2014
    Availability
    Academic Institutions and Nonprofits only
    52319-plasmid-map-sequence-id-237583

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  9. pET-pAG-Hia5

    Plasmid
    #174373
    Purpose
    Plasmid for IPTG-inducible expression and purification of protein A/G-Hia5-6xHis (pAG-Hia5) for antibody directed DNA (adenine) methylation (as in DiMeLo-seq)
    Depositor
    Aaron Straight
    Article
    Altemose et al Nat Methods. 2022 Jun;19(6):711-723. doi
    Insert
    protein AG - hia5
    Use
    Tags
    6xHis and Protein A/G
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    Sept. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    174373-plasmid-map-sequence-id-341861

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  16. pET28a SnoopTag-MBP

    Plasmid
    #72323
    Purpose
    Bacterial expression of Maltose Binding Protein linked to SnoopTag, a peptide tag forming a spontaneous covalent bond with its protein partner Snoopcatcher.
    Depositor
    Mark Howarth
    Article
    Veggiani et al Proc Natl Acad Sci U S A. 2016 Jan 19. p
    Insert
    pET28a SnoopTag-MBP
    Use
    Tags
    N-terminal His6 tag. MBP is fused at the C-termin…
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    Feb. 12, 2016
    Availability
    Academic Institutions and Nonprofits only
    72323-plasmid-map-sequence-id-137256

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  18. pET_RTX(exo-)-6xHis

    Plasmid
    #145028
    Purpose
    pET vector for IPTG-inducible, T7-based expression of the exonuclease deficient version of RTX bearing a C-terminal 6xHis tag
    Depositor
    Andrew Ellington
    Article
    Bhadra et al BioProtocol 2021
    Insert
    RTX(exo-)
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    N210D
    Promoter
    T7
    Available Since
    May 7, 2020
    Availability
    Academic Institutions and Nonprofits only
    145028-plasmid-map-sequence-id-292638

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  26. pET21a-alpha-synuclein

    Plasmid
    #51486
    Purpose
    Inducible expression of human alpha-synuclein in E.coli
    Depositor
    Michael J Fox Foundation MJFF
    Insert
    alpha-synuclein (SNCA Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available Since
    March 31, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    51486-plasmid-map-sequence-id-139447

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  36. pET-28a(+)-FGF2-G3

    Plasmid
    #135521
    Purpose
    Engineered form of fibroblast growth factor 2 with improved thermostability
    Depositor
    Paul Burridge
    Article
    Kuo et al Stem Cell Reports. 2020 Jan 9. pii: S221
    Insert
    fibroblast growth factor 2 (FGF2 Human)
    Use
    Tags
    6x His tag and Agg Thrombin Tag
    Expression
    Bacterial
    Mutation
    Codon optimized for E. Coli production
    Promoter
    T7 Promoter
    Available Since
    Jan. 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    135521-plasmid-map-sequence-id-273044

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  39. pET21a-Streptavidin-Dead

    Plasmid
    #20859
    Purpose
    Dead subunit for creating monovalent streptavidin with a single femtomolar biotin binding site. Use in combination with Streptavidin-Alive (construct 20860).
    Depositor
    Alice Ting
    Article
    Howarth et al Nat Methods. 2006 Apr . 3(4):267-73.
    Insert
    Streptavidin-Dead
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Triple mutant of wt Streptavidin N23A,S27D,S45A
    Promoter
    Available Since
    April 21, 2009
    Availability
    Academic Institutions and Nonprofits only
    20859-plasmid-map-sequence-id-220527

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  48. pETM6-E12-vioABECD

    Plasmid
    #66537
    Purpose
    pETM6 vector containing vioA-vioB-vioE-vioC-vioD in monocistronic configuration with optimized promoters (E12)
    Depositor
    Mattheos Koffas
    Article
    Jones et al Sci Rep. 2015 Jun 11;5:11301. doi: 10.10
    Inserts
    VioA
    VioB
    VioE
    VioC
    VioD
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Mutant T7 Promoter - G6 and Mutant T7 Promoter - …
    Available Since
    July 16, 2015
    Availability
    Academic Institutions and Nonprofits only
    66537-plasmid-map-sequence-id-114568

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Showing: 1651 - 1700 of 15033 results