164,097 results

1. pCW-HA-hRb-delta-CDK-puro

   Plasmid

   #212673

   Purpose

   Express tagged hRb delta-CDK phosphosite mutant

   Depositor

   Tobias Meyer, Yumi Konagaya

   Article

   Konagaya et al Nature. 2024 Jul;631(8020):424-431. doi:

   Insert

   Rb (RB1 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mutation

   All 15 CDK phospho-sites are mutated to alanines

   Promoter

   TRE

   Available since

   Aug. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-hSyn-hM3D(Gq)-mCherry (AAV8)

   Viral Prep

   #50474-AAV8

   Purpose

   Ready-to-use AAV8 particles produced from pAAV-hSyn-hM3D(Gq)-mCherry (#50474). In addition to the viral particles, you will also receive purified pAAV-hSyn-hM3D(Gq)-mCherry plasmid DNA. hSyn-driven hM3D(Gq) receptor with an mCherry reporter for CNO-induced neuronal activation. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Bryan Roth

   Promoter

   Syn

   Tags

   mCherry

   Available since

   Oct. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

3. mTagGFP2-Clathrin-15

   Plasmid

   #56413

   Purpose

   Localization: Clathrin Vesicles, Excitation: 483, Emission: 506

   Depositor

   Michael Davidson

   Insert

   Clathrin (CLTB Human)
   

   Use

   Tags

   mTagGFP2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 28, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

4. mRuby2-pBAD

   Plasmid

   #54771

   Purpose

   Localization: Protein Expression Vector , Excitation: 559, Emission: 600

   Depositor

   Michael Davidson

   Article

   Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

   Type

   Empty backbone

   Use

   Tags

   6xHis and mRuby2

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 25, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. mEGFP-Keratin-17

   Plasmid

   #56465

   Purpose

   Note- This plasmid contains Keratin 18. Localization: Cytokeratin, Excitation: 488, Emission: 507

   Depositor

   Michael Davidson

   Insert

   Keratin (KRT18 Human)
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   V282L

   Promoter

   CMV

   Available since

   Dec. 23, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pAPM-miR30-IFIH1-ts2

   Plasmid

   #174260

   Purpose

   IFIH1 knockdown

   Depositor

   Jeremy Luban

   Article

   Guney et al Proc Natl Acad Sci U S A. 2024 Jul 16;12

   Insert

   IFIH1 shRNA (IFIH1 Human)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mutation

   Promoter

   SFFV

   Available since

   Aug. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1 (-) hYAP 1-2alpha

   Plasmid

   #124143

   Purpose

   Protein expression for funtional studies of cell growth promotion

   Depositor

   Megan Finch-Edmondson, Marius Sudol

   Article

   Gaffney et al Gene. 2012 Nov 10;509(2):215-22. doi: 10

   Insert

   YAP1 (YAP1 Human)
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   None (Wild Type)

   Promoter

   Available since

   April 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. SARS-CoV-2_N_FL

   Plasmid

   #157867

   Purpose

   express MBP tagged SARS-CoV-2 Nucleocapsid protein

   Depositor

   Nicolas Fawzi

   Article

   Perdikari et al EMBO J. 2020 Nov 17:e106478. doi: 10.152

   Insert

   N (N SARS-CoV-2)
   

   Use

   Tags

   MBP

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLenti-sgRNA(lib)-puro

   Plasmid

   #119976

   Purpose

   Lenti sgRNA cloning backbone with CMV-puro resistance marker. Contains BsmBI sites for insertion of spacer sequences.

   Depositor

   Wensheng Wei

   Article

   Liu et al Nat Biotechnol. 2018 Nov 5. pii: nbt.428

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLVX-Puro-TDP-43-K263E

    Plasmid

    #133758

    Purpose

    Lentiviral expression of human TDP-43 K263E disease mutant in mammalian cells.

    Depositor

    Shawn Ferguson

    Article

    Roczniak-Ferguson et al Life Sci Alliance. 2019 Sep 16;2(5). pii

    Insert

    TDP-43 (TARDBP Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Changed lysine 263 to glutamate.

    Promoter

    CMV

    Available since

    Nov. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSBbi_RP_3XNLS-MicroID2-FLAG

    Plasmid

    #188523

    Purpose

    Encodes the biotin proximity labeling enzyme MicroID2 with a 3X nuclear localization sequence (NLS) for expression and stable integration into mammalian cells

    Depositor

    James Londino

    Article

    Johnson et al Mol Cell Proteomics. 2022 Jun 7:100256.

    Insert

    3XNLS-MicroID2
    

    Use

    Transposon mediated gene integration

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a/RPBSA

    Available since

    Sept. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. DJ-1 pET15b

    Plasmid

    #60678

    Purpose

    Recombinant protein expression of Park7

    Depositor

    Mark Wilson

    Article

    Lakshminarasimhan et al Biochemistry. 2008 Feb 5;47(5):1381-92.

    Insert

    DJ-1 (PARK7 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

13. pBS-MXT GIRK2

    Plasmid

    #20675

    Depositor

    Henry Lester

    Article

    Silverman et al J Biol Chem. 1996 Nov 29. 271(48):30524-

    Insert

    Mus musculus G protien coupled inward rectifier K+ channel 2 (Kcnj6 Mouse)
    

    Use

    Xenopus expression

    Tags

    3' UTR Xenopus B- globin and 5' UTR Xen…

    Expression

    Mutation

    A369V

    Promoter

    Available since

    March 31, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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14. plenti-UbcP-FLAG-CRBN-pGK-HYG

    Plasmid

    #107374

    Purpose

    Lentiviral expression of FLAG-CRBN

    Depositor

    William Kaelin

    Article

    Lu et al Science. 2014 Jan 17;343(6168):305-9. do

    Insert

    cereblon (CRBN Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    UbcP

    Available since

    May 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q36

    Plasmid

    #111745

    Purpose

    Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells

    Depositor

    Cheryl Arrowsmith

    Article

    Harding et al J Biol Chem. 2019 Apr 26;294(17):6986-70

    Insert

    HTT (HTT Human)
    

    Use

    Baculovirus expression

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Q36 (polyQ repeat)

    Promoter

    CMV and P10

    Available since

    Aug. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-EF1a-double floxed-Gat1-HA-WPRE-HGHpA

    Plasmid

    #184635

    Purpose

    Expresses the GABA membrane transporter Gat1 fused to HA, driven by the Ef1a promoter, in a Cre-dependent fashion

    Depositor

    Nicolas Tritsch

    Article

    Melani et al Cell Rep. 2022 Apr 19;39(3):110716. doi:

    Insert

    Gat1 (Slc6a1 Mouse)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Aug. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q30

    Plasmid

    #111744

    Purpose

    Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells

    Depositor

    Cheryl Arrowsmith

    Article

    Harding et al J Biol Chem. 2019 Apr 26;294(17):6986-70

    Insert

    HTT (HTT Human)
    

    Use

    Baculovirus expression

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Q30 (polyQ repeat)

    Promoter

    CMV and P10

    Available since

    Aug. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. mEmerald-Rab11a-7

    Plasmid

    #54245

    Purpose

    Localization: Ras GTPase, Excitation: 487, Emission: 509

    Depositor

    Michael Davidson

    Insert

    Rab11a (RAB11A Human)
    

    Use

    Tags

    mEmerald

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 16, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

19. pBS-PtH2B

    Plasmid

    #133390

    Purpose

    Piggybac vector for SRIRACCHA-mediated mutation enrichment (donor repair template for HDR)

    Depositor

    Blair Madison

    Article

    Wen et al J Biol Chem. 2017 Apr 14;292(15):6148-61

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    No promoter

    Available since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pETM33_SUFU_Sufu

    Plasmid

    #178488

    Purpose

    Bacterial expression of human domain with His-tag and GST tag

    Depositor

    Ylva Ivarsson

    Article

    Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

    Insert

    SUFU_Sufu (SUFU Human, Synthetic)
    

    Use

    Tags

    His-GST

    Expression

    Bacterial

    Mutation

    Promoter

    T7/LacO

    Available since

    March 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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