We narrowed to 15,033 results for: pET

1. pETM43 p62 FL (1-440)

   Plasmid

   #210425

   Purpose

   Bacterial expression of MBP-p62 FL (1-440) with 3C cleavage site

   Depositor

   Carsten Sachse

   Article

   Tarafder et al Methods Mol Biol. 2019;1880:3-15. doi: 1

   Insert

   p62 FL (NUP62 Human)
   

   Use

   Tags

   6xHis and MBP

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Feb. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pET28_6xHis-PARP1-Zn2

   Plasmid

   #174805

   Purpose

   Bacterial expression of isolated PARP1 Zn2 domain

   Depositor

   Joan Conaway

   Article

   Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

   Insert

   PARP1-Zn2 (PARP1 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET28_6xHis-PARP1-Zn3

   Plasmid

   #174806

   Purpose

   Bacterial expression of isolated PARP1 Zn3 domain

   Depositor

   Joan Conaway

   Article

   Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

   Insert

   PARP1-Zn3 (PARP1 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET28a SpyCatcher-SnoopCatcher

   Plasmid

   #72324

   Purpose

   Bacterial expression of SpyCatcher linked to SnoopCatcher.

   Depositor

   Mark Howarth

   Article

   Veggiani et al Proc Natl Acad Sci U S A. 2016 Jan 19. p

   Insert

   pET28a SpyCatcher-SnoopCatcher
   

   Use

   Tags

   N-terminal His6 tag

   Expression

   Bacterial

   Mutation

   35 amino acids at the N-term of SpyCatcher have b…

   Promoter

   Available Since

   Feb. 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pET28-His6-Ubc9

   Plasmid

   #133909

   Purpose

   Bacterial expression of His6-Ubc9

   Depositor

   Jeffrey Bode

   Article

   Hofmann et al Nat Chem. 2020 Nov;12(11):1008-1015. doi

   Insert

   SUMO-conjugating enzyme UBC9 (UBE2I Human)
   

   Use

   Tags

   Thrombin-cleavable His6-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Sept. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pET28a-mAmetrine-CNA35

   Plasmid

   #61604

   Purpose

   bacterial expression of N-terminal fusion protein of CNA35 with mAmetrine

   Depositor

   Maarten Merkx

   Article

   Aper et al PLoS One. 2014 Dec 9;9(12):e114983. doi:

   Insert

   mAmetrine and CNA35
   

   Use

   Tags

   His-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   March 23, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pETM6-T7-VHH-GFPminimizer

   Plasmid

   #202488

   Purpose

   Expresses the nanobody GFP minimizer with T7 induction

   Depositor

   Michael Lynch

   Article

   Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

   Insert

   nanobody GFP minimizer
   

   Use

   Tags

   6XHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Nov. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET28a-MH6-EsCas13d

   Plasmid

   #108303

   Purpose

   Expresses E. coli codon optimized EsCas13d in the pET28a backbone.

   Depositor

   Arbor Biotechnologies

   Article

   Yan et al Mol Cell. 2018 Mar 9. pii: S1097-2765(18

   Insert

   EsCas13d
   

   Use

   CRISPR

   Tags

   mH6 tag

   Expression

   Bacterial

   Mutation

   Promoter

   lac

   Available Since

   April 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET-PhoCl1-6His

   Plasmid

   #164033

   Purpose

   Expressed PhoCl1 in a pET expression vector

   Depositor

   Robert Campbell

   Article

   Lu et al Chem Sci. 2021 May 31;12(28):9658-9672.

   Insert

   PhoCl1
   

   Use

   Tags

   6xHis Tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promotor

   Available Since

   Feb. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. FL MALT1 WT in pET29b-Strep

    Plasmid

    #48968

    Purpose

    Expresses Full length wild type MALT1 in e.coli

    Depositor

    Guy Salvesen

    Article

    Hachmann et al Biochem J. 2012 Apr 1;443(1):287-95. doi

    Insert

    MALT1 isoform A (MALT1 Human)
    

    Use

    Tags

    His and Strep

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Nov. 6, 2013

    Availability

    Academic Institutions and Nonprofits only

11. pET28a-MH6-RspCas13d_RspCasWYL1

    Plasmid

    #108304

    Purpose

    Expresses E. coli codon optimized RspCas13d and RspCasWYL1 in the pET28a backbone.

    Depositor

    Arbor Biotechnologies

    Article

    Yan et al Mol Cell. 2018 Mar 9. pii: S1097-2765(18

    Inserts

    RspCas13d
    RspWYL1
    

    Use

    CRISPR

    Tags

    mH6 tag

    Expression

    Bacterial

    Mutation

    Promoter

    lac

    Available Since

    April 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET21a-TEX264_wt-His6

    Plasmid

    #220214

    Purpose

    Bacterial expression of wild type TEX264, C-terminal His6 tag

    Depositor

    Kristijan Ramadan

    Article

    Fielden et al Nat Commun. 2020 Mar 9;11(1):1274. doi:

    Insert

    TEX264 (TEX264 Human)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Aug. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET21-Streptavidin-K121R

    Plasmid

    #89880

    Purpose

    Streptavidin that has unimpaired biotin binding after dye labeling

    Depositor

    Mark Howarth

    Article

    Jacobsen et al Cell Chem Biol. 2017 Jul 17. pii: S2451-

    Insert

    Streptavidin-K121R-Glutamate_Tag
    

    Use

    Tags

    6 glutamate tag (C terminal on insert)

    Expression

    Bacterial

    Mutation

    K121R

    Promoter

    Available Since

    Aug. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pET-MBP-Geo_st

    Plasmid

    #87700

    Purpose

    Expression plasmid for Cas9 from Geobacillus stearothermophilus with an N-Term MBP

    Depositor

    Jennifer Doudna

    Insert

    GeoCas9
    

    Use

    Tags

    10xHis-MBP-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    June 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET28b-His-hCE

    Plasmid

    #112710

    Purpose

    Expresses N-terminally His-tagged human CE/RNGTT in bacterial cells

    Depositor

    Daniel Schoenberg

    Article

    Trotman et al Nucleic Acids Res. 2017 Oct 13;45(18):10

    Insert

    RNGTT (RNGTT Human)
    

    Use

    Tags

    6xHis and Stop codons placed after RNGTT sequence…

    Expression

    Bacterial

    Mutation

    R594H

    Promoter

    T7

    Available Since

    Aug. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET21d-myc-BirA

    Plasmid

    #109424

    Purpose

    Bacterial expression of biotin ligase (BirA) with an N-terminal myc epitope

    Depositor

    Martin Spiess

    Article

    Buser et al Proc Natl Acad Sci U S A. 2018 Jul 3;115

    Insert

    BirA (birA )
    

    Use

    Tags

    myc

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    June 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET22b-6h-GST-TEVp

    Plasmid

    #172887

    Purpose

    Codon optimized (E. coli) TEVp fused with GST and his-tagged for purification

    Depositor

    Urartu Seker

    Article

    Ahan et al ACS Synth Biol. 2019 Apr 19;8(4):686-696

    Insert

    TEV protease
    

    Use

    Tags

    GST solubility tag and HHHHHH

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available Since

    July 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pETM6-H10-mCherry

    Plasmid

    #66531

    Purpose

    Expresses mCherry under the control of Mutant T7 Promoter H10

    Depositor

    Mattheos Koffas

    Article

    Jones et al Sci Rep. 2015 Jun 11;5:11301. doi: 10.10

    Insert

    mCherry
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Codon Optimized for E. coli

    Promoter

    Mutant T7 Promoter - H10

    Available Since

    June 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET23b-Casp9-His

    Plasmid

    #11829

    Depositor

    Guy Salvesen

    Article

    Stennicke et al J Biol Chem. 1999 Mar 26. 274(13):8359-6

    Insert

    Caspase 9 (CASP9 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    May 12, 2006

    Availability

    Academic Institutions and Nonprofits only

20. pET28a Halotag-CNA35

    Plasmid

    #131128

    Purpose

    Halotag-CNA35 peptide for Collagen labelling

    Depositor

    Samantha Stehbens

    Article

    Ju et al bioRxiv 2022

    Insert

    Halotag-CNA35
    

    Use

    Tags

    Halotag

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    May 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pET28a-Ec.coaBC (pCLK1210)

    Plasmid

    #50448

    Purpose

    Heterologous expression of E. coli CoA biosynthetic enzyme, PPCS_PPCDC - phosphopantothenoylcysteine synthetase_phosphopantothenoylcysteine decarboxylase

    Depositor

    Tadhg Begley, Erick Strauss

    Article

    Strauss et al J Biol Chem. 2001 Apr 27;276(17):13513-6

    Insert

    PPCS PPCDC
    

    Use

    Tags

    6XHIS tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    March 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pET28a-MH6-RspCas13d

    Plasmid

    #108305

    Purpose

    Expresses E. coli codon optimized RspCas13d in the pET28a backbone.

    Depositor

    Arbor Biotechnologies

    Article

    Yan et al Mol Cell. 2018 Mar 9. pii: S1097-2765(18

    Insert

    RspCas13d
    

    Use

    CRISPR

    Tags

    mH6 tag

    Expression

    Bacterial

    Mutation

    Promoter

    lac

    Available Since

    April 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pETduet+sfGFP-TetR

    Plasmid

    #157794

    Purpose

    Expression of sfGFP-TetR fusion protein

    Depositor

    Michael Rosen

    Article

    Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

    Insert

    sfGFP-TetR
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Oct. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pET28-SBP-TEV

    Plasmid

    #36943

    Purpose

    SGC Empty backbone for bacterial expression

    Depositor

    Cheryl Arrowsmith

    Type

    Empty backbone

    Use

    Tags

    6xHistag-SBP-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    June 19, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

25. pET28a-SnoopTagJr-MBP

    Plasmid

    #105628

    Purpose

    Plasmid for recombinant expression in bacteria of MBP fused to SnoopTagJr, permitting covalent linkage to DogTag by SnoopLigase

    Depositor

    Mark Howarth

    Article

    Buldun et al J Am Chem Soc. 2018 Feb 28;140(8):3008-3

    Insert

    SnoopTagJr-MBP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    March 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pET24a-AlkB deltaN11

    Plasmid

    #73622

    Purpose

    Expresses E. coli AlkB absent the first 11 amino acids and with a C-terminal histidine tag

    Depositor

    Catherine Drennan

    Article

    Frick et al Proc Natl Acad Sci U S A. 2007 Jan 16;10

    Insert

    AlkB (alkB E. coli)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    deletion of first 11 AA

    Promoter

    T7

    Available Since

    Feb. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

27. pET-6xHis-GFP(+30)

    Plasmid

    #199164

    Purpose

    used to express a supercharged GFP variant in E. coli

    Depositor

    Allie Obermeyer

    Article

    Yeong et al ACS Cent Sci. 2020 Dec 23;6(12):2301-231

    Insert

    GFP(+30)
    

    Use

    Tags

    MGHHHHHHGGA

    Expression

    Mutation

    E16R, V21K, D29K, E42K, E44K, D86K, E100K, D112K,…

    Promoter

    T7

    Available Since

    May 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pET28a_His-TEV-EiCsm6

    Plasmid

    #172487

    Purpose

    Bacterial expression of codon-optimized His-EiCsm6 with a TEV protease cleavage site.

    Depositor

    Jennifer Doudna

    Article

    Liu et al Nat Chem Biol. 2021 Sep;17(9):982-988. d

    Insert

    Enterococcus italicus Csm6 (codon optimized)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter and lac operator

    Available Since

    Aug. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pET15b CnA CnB

    Plasmid

    #11787

    Depositor

    Anjana Rao

    Article

    Mondragon et al Biochemistry. 1997 Apr 22. 36(16):4934-4

    Insert

    CnA CnB (PPP3R1, PPP3CA Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    May 4, 2006

    Availability

    Academic Institutions and Nonprofits only

30. pET11b-SpyCatcher-HA2

    Plasmid

    #165991

    Purpose

    SpyCatcher-fused influenza HA ministem with removable HisTag

    Depositor

    Vesa Hytönen

    Article

    Lampinen et al J Nanobiotechnology. 2021 Jan 19;19(1):2

    Insert

    SpyCatcher-HA2
    

    Use

    Tags

    Foldon, HisTag, and SpyCatcher

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    March 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pET29b Tau Cys

    Plasmid

    #108867

    Purpose

    Expresses human Tau with a mutated cysteine

    Depositor

    Gabriele Kaminski Schierle

    Insert

    Tau (MAPT Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    I260C C291A C322A to allow maleimide dye labelling

    Promoter

    T7

    Available Since

    May 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pET28a-MH6-RspWYL1

    Plasmid

    #108306

    Purpose

    Expresses E. coli codon optimized RspWYL1 in the pET28a backbone.

    Depositor

    Arbor Biotechnologies

    Article

    Yan et al Mol Cell. 2018 Mar 9. pii: S1097-2765(18

    Insert

    RspWYL1
    

    Use

    CRISPR

    Tags

    mH6 tag

    Expression

    Bacterial

    Mutation

    Promoter

    lac

    Available Since

    April 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. JT113-pETDuet1-(R)-hREV3L

    Plasmid

    #64872

    Purpose

    bacterial expression of human REV3L

    Depositor

    Richard Wood

    Article

    Tomida et al Nucleic Acids Res. 2015 Jan;43(2):1000-1

    Insert

    REV3L (REV3L Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    July 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pET11a-4SS-HEWL

    Plasmid

    #39233

    Depositor

    Harald Schwalbe

    Article

    Schlorb et al J Biomol NMR. 2005 Oct;33(2):95-104.

    Insert

    Hen egg white lysozyme (LYZ Chicken)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    N-terminal Met

    Promoter

    T7

    Available Since

    Sept. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pET21b-Casp1-His

    Plasmid

    #11809

    Depositor

    Guy Salvesen

    Insert

    Caspase-1 (CASP1 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    May 12, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pET-His-VCre

    Plasmid

    #123131

    Purpose

    Bacterial expression plasmid for wild-type VCre with an N-terminal His-tag.

    Depositor

    David Liu

    Article

    Bessen et al Nat Commun. 2019 Apr 26;10(1):1937. doi:

    Insert

    VCre recombinase
    

    Use

    Cre/Lox

    Tags

    6x His tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    April 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pET28a-PKM2-R399E

    Plasmid

    #122691

    Purpose

    Express His-tagged PKM2 (R399E) protein in bacteria.

    Depositor

    Wen-Ching Wang

    Article

    Chen et al Commun Biol. 2019 Mar 15;2:105. doi: 10.

    Insert

    Pyruvate kinase M2 isoform (PKM Human)
    

    Use

    Tags

    6His-tag

    Expression

    Bacterial

    Mutation

    R399E

    Promoter

    Available Since

    March 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pET-21(+)Tm9D8* TrpB

    Plasmid

    #207517

    Purpose

    Expression of Tm9D8* TrpB for the synthesis of 7-Aza-L-Tryptophan (7AW) from 7-Azaindole (7AI) and serine

    Depositor

    Thomas Huber

    Article

    Abdelkader et al ACS Sens. 2023 Oct 27. doi: 10.1021/acss

    Insert

    Tryptophan synthase β-subunit
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Oct. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. His-SUMO-hDDI2_pET11a

    Plasmid

    #169120

    Purpose

    expression of protein

    Depositor

    Barbara Dirac-Svejstrup

    Article

    Dirac-Svejstrup et al Mol Cell. 2020 Jul 16;79(2):332-341.e7.

    Insert

    hDDI (DDI2 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pET28a HAT epitope

    Plasmid

    #129445

    Purpose

    expression of HAT epitope

    Depositor

    Oskar Laur

    Insert

    HAT epitope
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pET41b+_Ufd1-His

    Plasmid

    #117107

    Purpose

    Production of recombinant Ufd1-His in E.coli

    Depositor

    Hemmo Meyer

    Article

    Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

    Insert

    Ufd1 (UFD1 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pET28a-IMX-DogTag

    Plasmid

    #113765

    Purpose

    Expresses IMX-DogTag in bacterial cells for modular antigen multimerization using SnoopLigase

    Depositor

    Mark Howarth

    Article

    Andersson et al Sci Rep. 2019 Mar 15;9(1):4625. doi: 10.

    Insert

    IMX-DogTag
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    March 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. LOV-Turbo-His6_pET21a

    Plasmid

    #199668

    Purpose

    expresses LOV-Turbo in bacteria

    Depositor

    Alice Ting

    Article

    Lee et al Nat Methods. 2023 May 15. doi: 10.1038/s

    Insert

    LOV-Turbo
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    May 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pET28a-PKM2-H391Y

    Plasmid

    #122692

    Purpose

    Express His-tagged PKM2 (H391Y) protein in bacteria.

    Depositor

    Wen-Ching Wang

    Article

    Chen et al Commun Biol. 2019 Mar 15;2:105. doi: 10.

    Insert

    Pyruvate kinase M2 isoform (PKM Human)
    

    Use

    Tags

    6His-tag

    Expression

    Bacterial

    Mutation

    H391Y

    Promoter

    Available Since

    March 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pET24a-LaM4-EGFP

    Plasmid

    #182641

    Purpose

    Bacterial expression of a functionalized anti-mCherry (LaM4) nanobody fused to EGFP. LaM4-EGFP also contains a T7, HA, BAP and His6 epitope

    Depositor

    Martin Spiess

    Article

    Buser et al Life Sci Alliance. 2022 Mar 21;5(7). pii

    Insert

    anti-mCherry nanobody fused to a T7, HA, BAP and His6 epitope
    

    Use

    Tags

    BAP, EGFP, HA, His6, and T7

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    April 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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46. pET-6xHis-mCherry(-6)

    Plasmid

    #205045

    Purpose

    Fluorescent protein with charge-patterned cationic peptide to promote biomolecular condensate formation in E. coli

    Depositor

    Allie Obermeyer

    Article

    Liao et al ACS Synth Biol. 2024 Feb 3. doi: 10.1021

    Insert

    mCherry(-6)
    

    Use

    Tags

    MGHHHHHGG

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Feb. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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47. pETDuet RagA-RagC

    Plasmid

    #99641

    Purpose

    Bacterial expression of wildtype Rag GTPase dimer

    Depositor

    David Sabatini, Kuang Shen

    Article

    Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

    Inserts

    RagA (RRAGA Human)
    RagC (RRAGC Human)
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Dec. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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48. pET-6xHis-GFP(+36)

    Plasmid

    #199165

    Purpose

    used to express a supercharged GFP variant in E. coli

    Depositor

    Allie Obermeyer

    Article

    Yeong et al ACS Cent Sci. 2020 Dec 23;6(12):2301-231

    Insert

    GFP(+36)
    

    Use

    Tags

    MGHHHHHHGGA

    Expression

    Mutation

    E16R, T19R, V21K, D29K, E42K, E44K, N49E, D86K, Q…

    Promoter

    T7

    Available Since

    May 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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49. pET28a-SUMO-MjDim1

    Plasmid

    #189936

    Purpose

    Expression of Mjdim1, a methyltransferase used in m6A-SAC-seq.

    Depositor

    Chuan He

    Article

    Hu et al Nat Biotechnol. 2022 Mar 14. pii: 10.103

    Insert

    rsmA (MJ_RS00455 Methanocaldococcus jannaschii)
    

    Use

    Tags

    6x His tag, FLAG tag, SUMO tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available Since

    Sept. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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50. pET-20b-M-Ntx-S1

    Plasmid

    #58716

    Purpose

    Expresses in Escherichia coli Notexin with a N-terminal extra methionine and with the amino acid substitution N1S

    Depositor

    Fiorella Tonello

    Article

    Simonato et al Toxicon. 2014 Jun 18. pii: S0041-0101(14

    Insert

    Notexin
    

    Use

    Tags

    Methionine

    Expression

    Bacterial

    Mutation

    Changed Asparagine 1 to Serine

    Promoter

    T7

    Available Since

    Aug. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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