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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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Showing: 1901 - 1950 of 11733 results

  17. pAAH5

    Plasmid
    #41829
    Purpose
    Used as a PCR template for tagging yeast proteins with the SNAP tag by homologous recombination and selection for hygromycin resistance.
    Depositor
    Melissa Moore
    Article
    Hoskins et al Science. 2011 Mar 11;331(6022):1289-95.
    Type
    Empty backbone
    Use
    Pcr template
    Expression
    Bacterial and Yeast
    Promoter
    T7,TEF
    Available Since
    Oct. 17, 2013
    Availability
    Academic Institutions and Nonprofits only
    41829-plasmid-map-sequence-id-69202

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  21. pDG335

    Plasmid
    #100899
    Purpose
    SpCas9n (D10A Nickase mutant) with a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of DSBs with no off-target cuts.
    Depositor
    Paul Thomas
    Article
    Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do
    Inserts
    humanized CRISPR associated protein 9 Nickase
    U6-gRNA scaffold 1
    U6-gRNA scaffold 2
    Use
    CRISPR and Mouse Targeting
    Tags
    HA
    Expression
    Mammalian
    Mutation
    D10A mutant converts to Nickase
    Promoter
    CBh and U6
    Available Since
    Dec. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    100899-plasmid-map-sequence-id-195324

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  22. pTUB1:YFP-AID-3HA, DHFR-TS:HXGPRT

    Plasmid
    #87260
    Purpose
    YFP-AID-3HA fusion driven by a minimal TUB1 promoter with an HXGPRT drug selectable marker. The AID CDS is Arabidopsis thaliana auxin-responsive protein IAA17.
    Depositor
    David Sibley
    Article
    Brown et al MBio. 2017 May 2;8(3). pii: e00375-17. d
    Inserts
    YFP-AID-3HA
    HXGPRT
    Use
    Toxoplasma expression
    Tags
    3HA and AID
    Promoter
    TgDHFR-TS 463 bp and TgTUB1 378 bp
    Available Since
    May 4, 2017
    Availability
    Academic Institutions and Nonprofits only
    87260-plasmid-map-sequence-id-176554

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  25. pCDH-EF1-FLPe

    Plasmid
    #72262
    Purpose
    Express FLPe under EF-1 promoter
    Depositor
    Kazuhiro Oka
    Insert
    FLPe
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    elongation factor-1
    Available Since
    Jan. 11, 2016
    Availability
    Industry, Academic Institutions, and Nonprofits
    72262-plasmid-map-sequence-id-131821

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  37. pGL2-hOct4 DE-SV40-Luc

    Plasmid
    #26279
    Purpose
    Addgene next-generation sequencing identified numerous modifications from the indicated plasmid backbone. Please see the sequences link for additional information.
    Depositor
    Rudolf Jaenisch
    Article
    Hanna et al Proc Natl Acad Sci U S A. 2010 May 18. 1
    Insert
    Oct4 Distal Enhancer Element (DE) (POU5F1 Human)
    Use
    Luciferase
    Expression
    Mammalian
    Mutation
    See Depositor Comments below
    Available Since
    Oct. 18, 2010
    Availability
    Academic Institutions and Nonprofits only
    26279-plasmid-map-sequence-id-244682

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  39. LD-GA

    Plasmid
    #209862
    Purpose
    Dimerization dependent fluorescent protein GA anchored to cytosolic face of the lipid droplet membrane with hairpin domain of human GPAT4
    Depositor
    Sarah Cohen
    Article
    Miner et al Contact (Thousand Oaks). 2024 Feb 4;7:25
    Insert
    ddGFP A
    Tags
    Hairpin domain of GPAT4 MNFQYISLRLTVLWGLGVLIRYCFL…
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Nov. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    209862-plasmid-map-sequence-id-415467

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  41. pJA5 H-2K(b)

    Plasmid
    #180441
    Purpose
    E. coli expression of soluble MHC protein
    Depositor
    John Altman
    Insert
    H-2K(b)
    Tags
    BSP41
    Expression
    Bacterial
    Mutation
    Replacement of transmembrane domain with enzymati…
    Available Since
    March 2, 2022
    Availability
    Academic Institutions and Nonprofits only
    180441-plasmid-map-sequence-id-355754

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  42. pAd5-B6/7 ∆E3-GFP

    Plasmid
    #179202
    Purpose
    A block for the AdenoBuilder genome assembly system. The insert consolidates the regions present in pAd5-B6∆E3-GFP and pAd5-B7.
    Depositor
    Fred Bunz
    Article
    Jang et al STAR Protoc. 2022 Jan 20;3(1):101123. do
    Insert
    Modified block 6/7, with deletion in E3 and insertion of GFP cassette
    Use
    Adenoviral
    Available Since
    Jan. 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    179202-plasmid-map-sequence-id-351756

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  46. pR I-Ab alpha BirA

    Plasmid
    #113638
    Purpose
    Insect cell expression vector for I-Ab alpha chain fused to BirA biotinylation site
    Depositor
    James Moon
    Article
    Moon et al Methods Mol Biol. 2018;1799:165-181. doi
    Insert
    I-Ab alpha BirA (H2-Ab1 Mouse)
    Tags
    BirA biotinylation signal site and fused to Fos-J…
    Expression
    Insect
    Mutation
    transmembrane and cytoplasmic domains truncated
    Promoter
    Metallothionein
    Available Since
    Sept. 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    113638-plasmid-map-sequence-id-221155

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Showing: 1901 - 1950 of 11733 results