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neurod1:cre; cmlc2:EGFP

Plasmid

#173480

Purpose

cre is driven by -5kb neurod1 promoter. The vector contain a cmlc2:EGFP maker

Depositor

John Rawls

Article

Ye et al Cell Host Microbe. 2021 Feb 10;29(2):179

Insert

Cre

Use

Zebrafish expression

Available Since

March 17, 2022

Availability

Academic Institutions and Nonprofits only

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pHMR1A

Plasmid

#102957

Purpose

gene cloning of MR1A

Depositor

Gennaro De Libero, Lucia Mori

Article

Lepore et al Nat Commun. 2014 May 15;5:3866. doi: 10.

Insert

MR1A cDNA (MR1 Human)

Use

Cloning

Mutation

Stop codon has been removed by HindIII site

Available Since

Nov. 20, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO/GFP-TTP

Plasmid

#141190

Purpose

Expresses GFP-TTP in mammalian cells, can be used to make inducible cell line

Depositor

Simon Bekker-Jensen

Article

Tollenaere et al Cell Rep. 2019 Mar 26;26(13):3511-3521.e

Insert

TTP (ZFP36 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

June 5, 2020

Availability

Academic Institutions and Nonprofits only

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TRE-UBC-H2-Kb SCT-T2A-GFP

Plasmid

#205454

Purpose

lentiviral plasmid for expression of mouse H2-Kb SCT

Depositor

Deepta Bhattacharya

Article

Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

Insert

H2-Kb SCT (H2-K1 Mouse)

Expression

Mammalian

Promoter

UBC

Available Since

Oct. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pU6-ACTA2_gRNA1-SpCas9-T2A-GFP

Plasmid

#126706

Purpose

to express Cas9 from S. pyogenes with 2A-EGFP and sgRNA targeting human ACTA2 locus at the end of the endogenous coding sequence

Depositor

Darrell Kotton

Article

Kwong et al Stem Cell Reports. 2019 Sep 10;13(3):499

Insert

ACTA2_gRNA1-SpCas9-T2A-GF (ACTA2 Human)

Use

CRISPR

Available Since

July 9, 2020

Availability

Academic Institutions and Nonprofits only

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pCI neo-hEST2

Plasmid

#1781

Depositor

Bob Weinberg

Article

Meyerson et al Cell 1997 Aug 22;90(4):785-95.

Insert

hTERT (TERT Human)

Expression

Mammalian

Available Since

June 20, 2005

Availability

Academic Institutions and Nonprofits only

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pAAV-U6/sgClta-sfGFP-Clta

Plasmid

#200261

Purpose

U6 promoter expressing sgClta. Clta homology arms, sfGFP knock-in.

Depositor

Justin Eyquem

Article

Nyberg et al Cell. 2023 Jan 19;186(2):446-460.e19. do

Insert

sfGFP flanked by homology arms targeting Clta, additional U6 promoter expressing sgClta

Use

AAV

Available Since

Dec. 13, 2023

Availability

Academic Institutions and Nonprofits only

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6xHsa.NFKB:d2EGFP

Plasmid

#239990

Purpose

Destabilized green fluorescent protein (d2EGFP) under transcriptional control of NF-kB activity

Depositor

Raquel Espin-Palazon

Article

Campbell et al Nat Commun. 2024 Sep 6;15(1):7787. doi:

Insert

Destabilized EGFP

Promoter

cfos minimal promoter

Available Since

June 27, 2025

Availability

Academic Institutions and Nonprofits only

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PB-tetO-NGN2

Plasmid

#197090

Purpose

PiggyBac plasmid with tet-inducible expression of transcription factors for cortical neuron differentiation

Depositor

Brian Wainger

Article

Held et al Cell Rep. 2023 Aug 30;42(9):113046. doi:

Insert

NGN2 (NEUROG2 Human)

Expression

Mammalian

Promoter

TRE3G

Available Since

Nov. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pLKO.TRC1.shmNsd1.1, puro

Plasmid

#114446

Purpose

Lentiviral vector for expression of shRNA sequence targeting mouse Nsd1

Depositor

Adrian Bracken

Article

Streubel et al Mol Cell. 2018 Apr 19;70(2):371-379.e5.

Insert

shNsd1.1 (Nsd1 Mouse)

Use

Lentiviral and RNAi

Available Since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pMJ114

Plasmid

#85995

Purpose

One-guide Perturb-seq vector backbone; modified bovine U6 promoter; original constant region

Depositor

Jonathan Weissman

Article

Adamson et al Cell. 2016 Dec 15;167(7):1867-1882.e21.

Inserts

sgGFP-NT2
puro-T2A-BFP

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

modified bovine U6-2

Available Since

Feb. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pLentiCMV Puro DEST p38KTRmCerulean3

Plasmid

#59155

Purpose

Lentiviral vector to express p38 KTR mCerulean3 under CMV promoter (With Puromycin Resistance)

Depositor

Markus Covert

Article

Regot et al Cell. 2014 Jun 19;157(7):1724-34. doi: 1

Insert

p38 Kinase Translocation Reporter (MAPK14 Human, Mouse)

Use

Lentiviral

Tags

mCerulean3

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 19, 2014

Availability

Academic Institutions and Nonprofits only

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pBabe JUND-HA neo

Plasmid

#58489

Purpose

mammalian expression of human JUND

Depositor

Kevin Janes

Article

Wang et al Nat Cell Biol. 2014 Apr;16(4):345-56. do

Insert

JUND (JUN Human)

Tags

HA

Expression

Mammalian

Available Since

May 5, 2016

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1 Utr230-EGFP-3XNLS

Plasmid

#58466

Purpose

Expresses a nuclear-targeted actin filament reporter, consisting of the first 230 amino acids of human utrophin, on a CMV promoter

Depositor

Dyche Mullins

Article

Belin et al Mol Biol Cell. 2013 Apr;24(7):982-94. do

Inserts

Utr230 with 3X glycine linker (UTRN Human)
3X NLS with 3X stop codons

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

April 24, 2015

Availability

Academic Institutions and Nonprofits only

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pAS_4xhybPylT A41AA C55A FLAG-G1 PylRS Y125A

Plasmid

#154773

Purpose

plasmid with 4xhybrid PylT cassette (Mx1201 G1 PylT hybrid mutant A41AA C55A) and G1 PylRS Y125A for amber suppression in transient or stable, piggybac-mediated, integration

Depositor

Simon Elsaesser

Article

Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi

Insert

G1 PylRS (MJ_RS07805 methanogenic archaeon mixed culture ISO4-G1)

Tags

FLAG

Expression

Mammalian

Mutation

Y125A in G1PylS, hybrid PylT with A41AA and C55A …

Promoter

EF1

Available Since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pMJ117

Plasmid

#85997

Purpose

One-guide Perturb-seq vector backbone; modified human U6 promoter; constant region 3

Depositor

Jonathan Weissman

Article

Adamson et al Cell. 2016 Dec 15;167(7):1867-1882.e21.

Inserts

EGFP-NT2_cr3
puro-T2A-BFP

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

modified human U6

Available Since

Feb. 2, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Venus-173-N-ORAI1

Plasmid

#87618

Purpose

N-terminal Venus (YFP) fragment fused to full-length ORAI1; for BiFC.

Depositor

Jin Zhang

Article

Hertel et al Cell Rep. 2016 Jan 12;14(2):390-400. doi

Insert

Venus173-N-ORAI1 (ORAI1 Human, Synthetic)

Tags

6xHis, T7 tag (gene 10 leader), and Xpress(TM) ta…

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pmCherry-MYRIP

Plasmid

#89586

Purpose

Expression plasmid for a positive control prey protein in NanoSPD 2.0 assays.

Depositor

Thomas Friedman

Article

Bird et al Mol Biol Cell. 2016 Dec 8. pii: mbc.E16-

Insert

MYRIP (Myrip Mouse)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

April 25, 2017

Availability

Academic Institutions and Nonprofits only

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pBABE-hygro p53 DD

Plasmid

#9058

Depositor

Bob Weinberg

Article

Hahn et al Mol Cell Biol 2002 Apr;22(7):2111-23.

Insert

p53 DD (Trp53 Mouse)

Use

Retroviral

Expression

Mammalian

Mutation

DD. Dominant negative mutant carries deletion of…

Available Since

Dec. 7, 2005

Availability

Academic Institutions and Nonprofits only

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pMJ179

Plasmid

#85996

Purpose

One-guide Perturb-seq vector backbone; modified mouse U6 promoter; constant region 2

Depositor

Jonathan Weissman

Article

Adamson et al Cell. 2016 Dec 15;167(7):1867-1882.e21.

Inserts

EGFP-NT2_cr2
puro-T2A-BFP

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

modified mouse U6

Available Since

Feb. 22, 2017

Availability

Academic Institutions and Nonprofits only

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