We narrowed to 988 results for: Streptococcus

1. pBXMCS-2 Pconstitutive-sgRNA(Sth3)_cpaA

   Plasmid

   #133344

   Purpose

   for constitutive expression of a single guide RNA from Streptococcus thermophilus #3 with a seed region that targets cpaA gene's promoter from Caulobacter crescentus

   Depositor

   Michael Laub

   Article

   Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:

   Insert

   sgRNA_cpaA
   

   Use

   CRISPR

   Promoter

   constitutive

   Available Since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBXMCS-2 Pconstitutive-sgRNA(Spa)_gcrA3

   Plasmid

   #133343

   Purpose

   for constitutive expression of a single guide RNA from Streptococcus pasteurianus with a seed region that targets gcrA gene's promoter from Caulobacter crescentus

   Depositor

   Michael Laub

   Article

   Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:

   Insert

   sgRNA_gcrA
   

   Use

   CRISPR

   Promoter

   constitutive

   Available Since

   Jan. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBXMCS-2 Pconstitutive-sgRNA(Sth3)_blaA

   Plasmid

   #133345

   Purpose

   for constitutive expression of a single guide RNA from Streptococcus thermophilus #3 with a seed region that targets blaA gene's promoter from Caulobacter crescentus

   Depositor

   Michael Laub

   Article

   Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:

   Insert

   sgRNA_blaA
   

   Use

   CRISPR

   Promoter

   constitutive

   Available Since

   Jan. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBXMCS-2 Pconstitutive-sgRNA(Sth3)_gcrA2

   Plasmid

   #133341

   Purpose

   for constitutive expression of a single guide RNA from Streptococcus thermophilus #3 with a seed region that targets gcrA gene's promoter on the template strand in Caulobacter crescentus

   Depositor

   Michael Laub

   Article

   Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:

   Insert

   sgRNA_gcrA
   

   Use

   CRISPR

   Promoter

   constitutive

   Availability

   Academic Institutions and Nonprofits only

   

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5. CMV-dCas9_puroR

   Plasmid

   #219825

   Purpose

   CMV-driven expression of dCas9 with puromycin resistance (Adapted from plasmid #68416)

   Depositor

   Philipp Maass

   Insert

   dCas9
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   D10A/H841A

   Promoter

   CMV

   Available Since

   July 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. SP-Cas9

   Plasmid

   #62731

   Purpose

   Expresses SP-Cas9 protein in bacterial cells

   Depositor

   Niels Geijsen

   Article

   D'Astolfo et al Cell. 2015 Apr 23;161(3):674-690. doi: 1

   Insert

   SP-CAS9
   

   Use

   CRISPR

   Tags

   His tag and NLS

   Expression

   Bacterial

   Mutation

   Codon optimazed for e.coli

   Promoter

   T7

   Available Since

   April 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEasyCas9

   Plasmid

   #184909

   Purpose

   Expresses the Streptococcus pyogenes Cas9 protein

   Depositor

   Jeferson Gross

   Article

   Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

   Insert

   Cas9
   

   Use

   CRISPR

   Expression

   Bacterial and Yeast

   Available Since

   Jan. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSHS207 - Bacterial expression plasmid for WT SpCas9

   Plasmid

   #101199

   Purpose

   Bacterial expression plasmid for WT SpCas9

   Depositor

   Jennifer Doudna, Keith Joung

   Article

   Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

   Insert

   SpCas9
   

   Tags

   10x His, MBP, and TEV site

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pPEPY-PF6-lacI

   Plasmid

   #85589

   Purpose

   Work as template for amplification of Streptococcus pneumoniae adapted lacI gene, which is flanked by up- and downstream sequence for integration in prsA locus in S. pneumoniae

   Depositor

   Jan-Willem Veening

   Article

   Liu et al Mol Syst Biol. 2017 May 10;13(5):931. do

   Insert

   lacI
   

   Expression

   Bacterial

   Promoter

   PF6

   Available Since

   Feb. 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEC3079 (p7INT-Pveg_lacI)

    Plasmid

    #218505

    Purpose

    integrative plasmid enabling constitutive expression of lacI in Streptococcus, targets the attP site of phage T12 in several S. pyogenes strains via an integrase

    Depositor

    Emmanuelle Charpentier

    Article

    Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

    Insert

    lacI (lacI Escherichia coli)
    

    Expression

    Bacterial

    Promoter

    Pveg

    Available Since

    July 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. CROPseq-EFS-HypaSpCas9-P2A-EGFP

    Plasmid

    #106964

    Purpose

    single-vector CROPseq system with high fidelity SpCas9 (HypaSpCas9) for use in the CROPseq CRISPR knockout screen

    Depositor

    Alex Hewitt

    Insert

    HypaSpCas9
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    EFS

    Available Since

    May 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. dCas9-miniTurbo

    Plasmid

    #219822

    Purpose

    EF1α-driven expression of dCas9 fused to miniTurbo with GFP (Adapted from plasmid #153209)

    Depositor

    Philipp Maass

    Insert

    dCas9
    

    Use

    CRISPR

    Tags

    miniTurbo

    Expression

    Mammalian

    Mutation

    D10A/H841A

    Promoter

    EF1a

    Available Since

    July 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. CROPseq-EFS-HypaSpCas9-P2A-puro

    Plasmid

    #106965

    Purpose

    single-vector CROPseq system with high fidelity SpCas9 (HypaSpCas9) for use in the CROPseq CRISPR knockout screen

    Depositor

    Alex Hewitt

    Insert

    HypaSpCas9
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    EFS

    Available Since

    April 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. CAG-ABEmax-P2A-EGFP-ires-puro

    Plasmid

    #121170

    Purpose

    CAG promoter driven expression of ABEmax base editor, EGFP and Puromycin resistance.

    Depositor

    Timo Otonkoski

    Insert

    ABEmax-P2A-EGFP-ires-puro
    

    Use

    CRISPR

    Tags

    P2A-EGFP-ires-puro

    Expression

    Mammalian

    Available Since

    July 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. lentiCRISPRv2_PROX1_1+3

    Plasmid

    #121176

    Purpose

    Lentiviral construct for the expression of Cas9 protein and puromycin resistance from EFS promoter and two guide RNAs targeting for deletion PROX1 start codon sequence.

    Depositor

    Timo Otonkoski

    Insert

    Cas9-P2A-puro and U6 promoters driven expression of gRNAs
    

    Use

    CRISPR and Lentiviral

    Tags

    P2A-puro

    Expression

    Mammalian

    Available Since

    July 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. CAG-FNLS-T2A-EGFP-ires-puro

    Plasmid

    #121169

    Purpose

    CAG promoter driven expression of FNLS BE3 base editor, EGFP and Puromycin resistance.

    Depositor

    Timo Otonkoski

    Insert

    FNLS-T2A-EGFP-ires-puro
    

    Use

    CRISPR

    Tags

    T2A-EGFP-ires-puro

    Expression

    Mammalian

    Available Since

    July 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. CAG-xFNLS-T2A-EGFP-ires-puro

    Plasmid

    #121171

    Purpose

    CAG promoter driven expression of xFNLS BE3 base editor, EGFP and Puromycin resistance.

    Depositor

    Timo Otonkoski

    Insert

    xFNLS-T2A-EGFP-ires-puro
    

    Use

    CRISPR

    Tags

    T2A-EGFP-ires-puro

    Expression

    Mammalian

    Available Since

    July 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pX458-tRNA-SpCas9

    Plasmid

    #195183

    Purpose

    Vector for transient Cas9 and EGFP expression. Small tRNA promoter for sgRNA cloning by GoldenGate.

    Depositor

    Stephanie Roessler

    Insert

    Cas9
    

    Use

    CRISPR

    Promoter

    tRNA-Gln

    Available Since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. lentiCRISPRv2_PROX1_1+4

    Plasmid

    #121177

    Purpose

    Lentiviral construct for the expression of Cas9 protein and puromycin resistance from EFS promoter and two guide RNAs targeting for deletion PROX1 start codon sequence.

    Depositor

    Timo Otonkoski

    Insert

    Cas9-P2A-puro and U6 promoters driven expression of gRNAs
    

    Use

    CRISPR and Lentiviral

    Tags

    P2A-puro

    Expression

    Mammalian

    Available Since

    March 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAGM4723:Ble:Cas9

    Plasmid

    #112208

    Purpose

    Golden Gate Level 2 construct encoding Cas9-YFP, Zeocin resistance.

    Depositor

    Thomas Mock

    Inserts

    Cas9
    SheBle
    

    Use

    CRISPR; Diatom, phaeodactylum

    Tags

    Phaeodactylum fcp promoter and terminator and YFP

    Mutation

    Phaeodactylum Fcp promoter

    Promoter

    Phaeodactylum Fcp

    Available Since

    July 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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