167,948 results

1. pCDNA3 Flag Jnk3a1

   Plasmid

   #13758

   Depositor

   Roger Davis

   Article

   Gupta et al EMBO J. 1996 Jun 3. 15(11):2760-70.

   Insert

   Jnk3a1 (MAPK10 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Available Since

   Feb. 22, 2007

   Availability

   Academic Institutions and Nonprofits only

2. pSIQ

   Plasmid

   #216238

   Purpose

   All-in One IQ transgene switch, empty vector

   Depositor

   Hyunju Ro

   Article

   Hong et al Mol Ther Methods Clin Dev. 2024 Feb 2;32

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Mammalian

   Available Since

   May 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. Arl13b-EGFP

   Plasmid

   #234844

   Purpose

   A mammalian expression plasmid encoding Arl13b with EGFP at C-terminus.

   Depositor

   Lei Lu

   Article

   Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

   Insert

   Arl13b (ARL13B Human)
   

   Expression

   Mammalian

   Available Since

   June 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAG-mGFP-Actin

   Plasmid

   #21948

   Depositor

   Ryohei Yasuda

   Article

   Murakoshi et al Brain Cell Biol. 2008 Aug . 36(1-4):31-4

   Insert

   betaActin (ACTB Human)
   

   Tags

   mEGFP

   Expression

   Mammalian

   Available Since

   Sept. 3, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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5. pfwB

   Plasmid

   #37329

   Depositor

   John Schiller

   Article

   Buck et al J Virol. 2005 Mar;79(5):2839-46.

   Insert

   GFP
   

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   July 20, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-DeAct-GS1

   Plasmid

   #89445

   Purpose

   Expresses EGFP-GS1 (Human Gelsolin 53-176) in mammalian cells

   Depositor

   Brad Zuchero

   Article

   Harterink et al Nat Methods. 2017 Apr 10. doi: 10.1038/n

   Insert

   DeAct-GS1 (GSN Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Truncation spanning cytoplasmic Gsn amino acids 5…

   Promoter

   CMV

   Available Since

   April 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. Factor IX donor

   Plasmid

   #182141

   Purpose

   Factor IX donor for twinPE and Bxb1 mediated insertion at ALB

   Depositor

   David Liu

   Article

   Anzalone et al Nat Biotechnol. 2022 May;40(5):731-740.

   Insert

   attP-Factor IX intron 1 and exon 2-8 CDS and exon 8 3'UTR
   

   Expression

   Mammalian

   Available Since

   April 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSRKBB-empty

   Plasmid

   #59449

   Purpose

   Contains BioBrick prefixed and suffixed insert with a modified lac promoter, Shine-Delgarno sequence, 5’ & 3’ MCS, 3’ 6xHis tag.

   Depositor

   Claudia Schmidt-dannert

   Type

   Empty backbone

   Expression

   Bacterial

   Promoter

   modified lac

   Available Since

   Sept. 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET296-YcpLac111 CYC1p-MCP-NLS-2xyeGFP

   Plasmid

   #104394

   Purpose

   Yeast MCP-GFP expression

   Depositor

   Evelina Tutucci, Robert Singer

   Article

   Tutucci et al Nat Methods. 2017 Nov 13. doi: 10.1038/n

   Insert

   MCP-NLS-2xyeGFP
   

   Expression

   Bacterial and Yeast

   Mutation

   MCP fused to NLS(SV40) and 2x yeast codon optimiz…

   Promoter

   CYC1

   Available Since

   Jan. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHAGE mNeonGreen-Core (HBc from HBV) IRES puro

    Plasmid

    #122202

    Purpose

    Plasmid coding for mNeonGreen-Core protein (from HBV AYW) and a puromycin resistance in a lentiviral backbone.

    Depositor

    Raphael Gaudin

    Article

    Hallez et al Nat Microbiol. 2019 Apr 1. pii: 10.1038/

    Inserts

    HBc from HBV AYW
    mNeonGreen
    

    Tags

    GGSGGSGGS linker

    Expression

    Mammalian

    Available Since

    April 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET21a-MTF-His

    Plasmid

    #124133

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Sebastian Maerkl, Takuya Ueda

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Methionyl-tRNA formyltransferase (fmt Escherichia coli)
    

    Tags

    6x-His

    Expression

    Bacterial

    Available Since

    April 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1/hChR2(H134R)-EYFP

    Plasmid

    #20940

    Purpose

    Mammalian expression of humanized ChR2 with H134R mutation fused to EYFP for optogenetic activation

    Depositor

    Karl Deisseroth

    Article

    Zhang et al Nature. 2007 Apr 5. 446(7136):633-9.

    Insert

    channelrhodopsin-2
    

    Expression

    Mammalian

    Mutation

    H134R mutation in ChR2

    Promoter

    CMV

    Available Since

    May 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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13. EF1a-MVPH

    Plasmid

    #235594

    Purpose

    Expresses the MS2-VP64-P65-HSF1 under the control of human EF1a promoter

    Depositor

    Ting Zhou

    Article

    Wu et al Stem Cell Reports. 2025 Apr 25:102499. d

    Insert

    MS2-VP64-P65-HSF1
    

    Expression

    Mammalian

    Mutation

    N55K in MS2

    Promoter

    EF1a

    Available Since

    May 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. tet-AID-PcrA M6-UGI-T2A-mTagBFP2-PGK-Puro-T2A-rtTA

    Plasmid

    #221234

    Purpose

    Tet-inducible lentiviral HACE Editor as a fusion of AID cytidine deaminase and PcrA helicase.

    Depositor

    Fei Chen

    Article

    Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

    Insert

    AID-PcrA M6-UGI-T2A-mTagBFP2
    

    Use

    Lentiviral

    Available Since

    Feb. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLV-Hygro-TRE>{MCS}:T2A:EGFP

    Plasmid

    #236250

    Purpose

    Hygromycin selected lentiviral vector with MCS for tetracycline-inducible gene, for use with dual-inducible CymR-CuO system

    Depositor

    Genevieve Kendall

    Article

    Kent et al Sci Rep. 2024 Sep 5;14(1):20773. doi: 10

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    TRE

    Available Since

    June 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-Ef1a-DIO-Synaptophysin-GCaMP6s (AAV5)

    Viral Prep

    #105715-AAV5

    Purpose

    Ready-to-use AAV5 particles produced from pAAV-Ef1a-DIO-Synaptophysin-GCaMP6s (#105715). In addition to the viral particles, you will also receive purified pAAV-Ef1a-DIO-Synaptophysin-GCaMP6s plasmid DNA. EF1a-driven, Cre-dependent GCaMP6s fused to the presynaptic protein synaptophysin. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Rylan Larsen

    Promoter

    Ef1a, Human elongation factor-1 alpha

    Available Since

    Oct. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

17. pLPCX2-MT1-MMP-eGFP

    Plasmid

    #89819

    Purpose

    Express in MT1-MMP mammalian cells

    Depositor

    Shengyu Yang

    Article

    Sun et al J Cell Biol. 2014 Nov 24;207(4):535-48.

    Insert

    MT1-MMP (MMP14 Human)
    

    Use

    Retroviral

    Tags

    eGFP

    Available Since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHAGE-TO-dCas9

    Plasmid

    #75381

    Purpose

    dCas9

    Depositor

    Thoru Pederson

    Article

    Ma et al Nat Biotechnol. 2016 Apr 18. doi: 10.103

    Insert

    Sp dCas9
    

    Use

    Lentiviral

    Tags

    no

    Expression

    Mammalian

    Promoter

    CMV-TO

    Available Since

    May 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pKLV-EF1a-mCherryLuciferase-W

    Plasmid

    #200101

    Purpose

    Lentiviral vector expressing mCherry fused with Luciferase at the C-terminus

    Depositor

    Kosuke Yusa

    Article

    Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

    Insert

    Luciferase
    

    Use

    CRISPR and Lentiviral

    Available Since

    Jan. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBS-ldhGFP

    Plasmid

    #27170

    Depositor

    Michela Lizier

    Article

    Lizier et al FEMS Microbiol Lett. 2010 Jul 1. 308(1):

    Insert

    lactate dehydrogenase promoter region fused with modified green fluorescent protein 5
    

    Expression

    Bacterial

    Available Since

    Jan. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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21. pET21b(+)-Is-PETase

    Plasmid

    #112202

    Purpose

    pET-21b(+) based plasmid for expression of PETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1), codon optimized for expression in E. coli K12

    Depositor

    Gregg Beckham, Christopher Johnson

    Article

    Austin et al Proc Natl Acad Sci U S A. 2018 May 8;115

    Insert

    PETase gene from Ideonella sakaiensis 201-F6, codon optimized for expression in E. coli K12
    

    Tags

    6XHIS

    Expression

    Bacterial

    Promoter

    N/A

    Available Since

    July 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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22. pET3a-hUb

    Plasmid

    #61937

    Purpose

    Expression of hUb in E.coli

    Depositor

    Chris Hill

    Article

    VanderLinden et al Mol Cell. 2015 Feb 17. pii: S1097-2765(1

    Insert

    human ubiquitin (UBC Human)
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

23. pDV10_pCMV-ADAR1-p150

    Plasmid

    #200405

    Purpose

    Expresses ADAR1 (p150 isoform) constitutively in mammalian cells

    Depositor

    James Collins

    Article

    Gayet et al Nat Commun. 2023 Mar 11;14(1):1339. doi:

    Insert

    ADAR-p150
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Available Since

    June 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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24. LsgRNA-MS2

    Plasmid

    #235597

    Purpose

    sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2. The cloning site is BsmbI.

    Depositor

    Ting Zhou

    Article

    Wu et al Stem Cell Reports. 2025 Apr 25:102499. d

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    May 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pDV09_pCMV-ADAR1-p110

    Plasmid

    #200404

    Purpose

    Expresses ADAR1 (p110 isoform) constitutively in mammalian cells

    Depositor

    James Collins

    Article

    Gayet et al Nat Commun. 2023 Mar 11;14(1):1339. doi:

    Insert

    ADAR-p110
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Available Since

    June 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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26. pLV_3xFLAG-ATP6V0A3

    Plasmid

    #228866

    Purpose

    Stable expression of V-ATPase subunit ATP6V0A3 with N-terminal 3xFLAG

    Depositor

    Wilhelm Palm

    Article

    Ratto et al Nat Commun. 2022 Aug 17;13(1):4848. doi:

    Insert

    ATP6V0A3 (TCIRG1 Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Available Since

    Nov. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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27. pLiLucB

    Plasmid

    #31596

    Depositor

    Michele Calos

    Article

    Woodard et al PLoS One. 2010 . 5(6):e11367.

    Insert

    alpha-1 antitrypsin promoter with an apolipoprotein enhancer (SERPINA1 Human)
    

    Use

    Luciferase

    Expression

    Mammalian

    Available Since

    Aug. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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28. pLVX-dL5**-mCER-TRF1

    Plasmid

    #168176

    Purpose

    Expresses FAP (dL5**) fused to mCerulean and the telomere binding protein TRF1

    Depositor

    Patricia Opresko

    Article

    Fouquerel et al Mol Cell. 2019 Jul 11;75(1):117-130.e6.

    Insert

    TRF1 (TERF1 Human)
    

    Use

    Lentiviral

    Tags

    FAP (dL5**) and mCerulean

    Expression

    Mammalian

    Available Since

    April 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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29. pCMV-THRA-RFP-EGFP

    Plasmid

    #228537

    Purpose

    Bichromatic fluorescent reporter of THRA isoform 1 and isoform 2 splicing in mammalian cells

    Depositor

    Markus Schuelke

    Article

    Graceffo et al Int J Mol Sci. 2024 Dec 17;25(24):13512.

    Insert

    Thyroid Hormone Receptor Alpha (THRA Human)
    

    Tags

    eGFP and tagRFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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30. pCAGGS-ChR2-Venus

    Plasmid

    #15753

    Purpose

    Expresses ChR2-Venus for neuronal excitation.

    Depositor

    Karel Svoboda

    Article

    Petreanu et al Nat Neurosci. 2007 May . 10(5):663-8.

    Insert

    Channelrhodopsin2-Venus
    

    Tags

    Venus

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Oct. 31, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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31. Multiplex CRISPR dCas9/FokI-dCas9 Accessory Pack

    Plasmid Kit

    #1000000062

    Purpose

    Accessory pack for construction of all-in-one CRISPR/Cas9 vectors expressing multiple gRNAs with either dCas9 or dCas9-FokI fusion; Kit #1000000055 is required to use this kit

    Depositor

    Takashi Yamamoto

    Available Since

    June 1, 2015

    Availability

    Academic Institutions and Nonprofits only

32. CXCR7-Tango

    Plasmid

    #66265

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    CXCR7 (ACKR3 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

33. ML1Nwt_in_pEGFP-C1

    Plasmid

    #67797

    Purpose

    Mammalian expression vector driving Mouse ML1Nwt (2xML1N domain wildtype version) fused to EGFP

    Depositor

    Rob Parton

    Article

    Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

    Insert

    ML1N*2-EGFP
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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34. BPK3079

    Plasmid

    #78741

    Purpose

    Human expression plasmid for AsCpf1 guide RNA (need to clone in spacer into BsmBI sites)

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2016 Jun 27. doi: 10.103

    Insert

    AsCpf1 crRNA backbone, without spacer sequence
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    June 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. CXCR7-DuET

    Plasmid

    #213224

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    CXCR7 (ACKR3 Human)
    

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Promoter

    CMV, T7

    Available Since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pSAD-F3-NPEST-iCRE-2A-mCherryPEST

    Plasmid

    #99608

    Purpose

    Self-inactivating DG-Rabies (SiR) expressing iCRE and destabilised mCherry

    Depositor

    Marco Tripodi

    Article

    Ciabatti et al Cell. 2017 Jul 1. pii: S0092-8674(17)306

    Inserts

    iCRE
    mCherry
    

    Use

    Unspecified

    Tags

    PEST

    Available Since

    Oct. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pCMV CEPIA4mt

    Plasmid

    #58220

    Purpose

    Green fluorescent indicator for calcium imaging in the mitochondria (low affinity variant)

    Depositor

    Masamitsu Iino

    Article

    Suzuki et al Nat Commun. 2014 Jun 13;5:4153. doi: 10.

    Insert

    CEPIA4mt
    

    Tags

    myc

    Expression

    Mammalian

    Mutation

    GCaMP2 N263Y E282V E334D M339L F395W D436E

    Promoter

    CMV

    Available Since

    July 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pcDNA3.1_nV5-AMOTL2

    Plasmid

    #172999

    Purpose

    constitutive expression of V5-tagged AMOTL2

    Depositor

    Michael Wehr

    Article

    Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

    Insert

    V5-AMOTL2 (AMOTL2 Human)
    

    Tags

    V5

    Expression

    Mammalian

    Available Since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pX601-mCherry

    Plasmid

    #84039

    Purpose

    Staphylococcus aureus (SaCas9) conjugated with mCherry

    Depositor

    Yuet Wai Kan

    Article

    Ye et al Proc Natl Acad Sci U S A. 2016 Sep 20;11

    Insert

    SaCas9
    

    Use

    AAV and CRISPR

    Tags

    NLS and T2A-mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. p6351 MSCV-CMV-CMV-Flag-HA-NSD3

    Plasmid

    #31357

    Depositor

    Peter Howley

    Article

    Rahman et al Mol Cell Biol. 2011 Jul . 31(13):2641-52

    Insert

    NSD3 (NSD3 Human)
    

    Tags

    FLAG and HA

    Expression

    Mammalian

    Available Since

    Oct. 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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