169,450 results

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N1-mSin1.2-GFP

Plasmid

#72908

Purpose

GFP-tagged mSin1 (isoform 2)

Depositor

Ivan Yudushkin

Article

Ebner et al J Cell Biol. 2017 Jan 31. pii: jcb.20161

Insert

Target of rapamycin complex 2 subunit MAPKAP1, isoform 2 (MAPKAP1 Human)

Tags

eGFP

Expression

Mammalian

Available Since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

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pXPR_120

Plasmid

#96917

Purpose

for CRISPRa, lentiviral expression of dCas9-VPR 2A BlastR

Depositor

John Doench, David Root

Article

Najm et al Nat Biotechnol. 2018 Feb;36(2):179-189.

Insert

dCas9

Use

Lentiviral

Tags

VPR

Expression

Mammalian

Mutation

D10A, N863A

Promoter

EF1a

Available Since

June 28, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV.Syn.GCaMP6f.WPRE.SV40 (AAV9)

Viral Prep

#100837-AAV9

Purpose

Ready-to-use AAV9 particles produced from pAAV.Syn.GCaMP6f.WPRE.SV40 (#100837). In addition to the viral particles, you will also receive purified pAAV.Syn.GCaMP6f.WPRE.SV40 plasmid DNA. Syn-driven GCaMP6f calcium sensor. These AAV preparations are suitable purity for injection into animals.

Depositor

Douglas Kim, GENIE Project

Article

Chen et al Nature. 2013 Jul 18;499(7458):295-300. d

Promoter

Syn

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only
YSBE 610 : pHYG_6xHA

Plasmid

#92091

Purpose

Plasmid for tagging proteins in Cryptococcus neoformans; 6xHA tag, HYG selection

Depositor

Yong-Sun Bahn

Article

So et al J Microbiol Biotechnol. 2017 Feb 28;27(2

Insert

6xHA

Use

tagging proteins in cryptococcus neoforman

Available Since

July 10, 2017

Availability

Academic Institutions and Nonprofits only

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pSEED-Dendra2

Plasmid

#219645

Purpose

Permits GoldenGate assembly (BsmBI) of donor plasmids to knock-in Dendra2 using the SEED/Harvest method

Depositor

Markus Affolter

Article

Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

Insert

Dendra2 SEED Cassette

Available Since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pSBbi 41BBL-CD86 mIL-15

Plasmid

#183245

Purpose

Expression of human 41BBL, CD86, and membrane bound human IL-15 (IL-15-IL-15Ra) in a Sleeping Beauty transposon vector for use in artificial antigen presenting cells

Depositor

Alexander McLellan

Article

Dobson et al Immunol Cell Biol. 2023 Aug 16. doi: 10.

Insert

41BBL, CD86, mbIL-15 (IL-15-IL-15Ra)

Expression

Mammalian

Available Since

Aug. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pLVX-TRE3G-mitoLbNOX-Flag

Plasmid

#234981

Purpose

Expresses mitochondrial LbNOX in mouse cell lines

Depositor

Denis Titov

Article

Choe et al Cell Chem Biol. 2025 Apr 17;32(4):620-63

Insert

mito-LbNOX

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Available Since

April 22, 2025

Availability

Academic Institutions and Nonprofits only

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Tau (wt) -VC

Plasmid

#87369

Purpose

expresses hTau fused to the C-terminal part of Venus

Depositor

Tiago Outeiro

Article

Blum et al Hum Mol Genet. 2015 Jan 1;24(1):76-85. d

Insert

MAPT (MAPT Human)

Tags

c-terminal part of Venus

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 20, 2017

Availability

Academic Institutions and Nonprofits only

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pAS3-rab-3P::AI::ChR2(H134R)

Plasmid

#232601

Purpose

Pan-neuronal expression of opsin 'ChR2(H134R)', regulated by rab-3 promoter and unc-54 3' UTR

Depositor

Andrew Leifer

Article

Sharma et al Genetics. 2024 Jul 8;227(3):iyae077. doi

Insert

Channelrhodopsin2

Expression

Worm

Mutation

H134R

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pMTU-DO-URA-C4

Plasmid

#160339

Purpose

High-copy expression vector for K. marxianus, uracil selection

Depositor

John Morrissey

Article

Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

Type

Empty backbone

Use

Kluyveromyces marxianus

Expression

Yeast

Promoter

n/a

Available Since

Nov. 23, 2020

Availability

Academic Institutions and Nonprofits only

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TFORF1482

Plasmid

#141828

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

TP63 (TP63 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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Golgi-targeted optoPLD

Plasmid

#140058

Purpose

Encodes CRY2-mCherry-PLD-P2A-CIBN-Sialyltransferase(TM) for mammalian expression

Depositor

Jeremy Baskin

Article

Tei et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

Phospholipase D

Tags

CIBN, CRY2, P2A, Sialyltransferase(TM), and mCher…

Expression

Mammalian

Available Since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

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TFORF1478

Plasmid

#143122

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

TP63 (TP63 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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GPI_2xmCherry

Plasmid

#127812

Purpose

Homo-dimer mCherry-mCherry expression vector with plasma membrane localization signal

Depositor

Salvatore Chiantia

Article

Dunsing et al Sci Rep. 2018 Jul 13;8(1):10634. doi: 10

Insert

glycosylphosphatidylinositol-mCherry-mCherry

Expression

Mammalian

Available Since

July 8, 2019

Availability

Academic Institutions and Nonprofits only

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3xmNeonGreen-3xrGBD

Plasmid

#176101

Purpose

Visualization of active Rho

Depositor

Dorus Gadella

Article

Mahlandt et al J Cell Sci. 2021 Aug 6. pii: 271216. doi

Insert

rhotekin (Rtkn Mouse)

Expression

Mammalian

Promoter

CMVdel

Available Since

Oct. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pCAGGS SARS-CoV-2 Alpha Spike

Plasmid

#185691

Purpose

Expresses codon-optimized full length SARS-CoV-2 Alpha Spike

Depositor

Marceline Côté

Article

Benlarbi et al iScience. 2022 Nov 18;25(11):105316. doi

Insert

SARS-CoV-2 Alpha Spike (S )

Expression

Mammalian

Mutation

Contains the following mutations: Δ69-70, Δ145, N…

Promoter

chicken β-actin promoter, CMV enhancer

Available Since

June 8, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pHR-FKBP:mCherry-BclXLc33

Plasmid

#72905

Purpose

Outer mitochondrial membrane-targeted mCherry-tagged recruiter construct

Depositor

Ivan Yudushkin

Article

Ebner et al J Cell Biol. 2017 Jan 31. pii: jcb.20161

Insert

Bl2-like protein 1, isoform Bcl-X(L) (fragment)

Use

Lentiviral

Tags

FKBP, mCherry

Expression

Mammalian

Mutation

33 C-terminal a.a.

Available Since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

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B1-INTEGRIN VN

Plasmid

#195216

Purpose

Mammalian expression of mCherry tagged B1 Integrin. Autoclustering mutant.

Depositor

David Bryant, Valerie Weaver

Article

Paszek et al Cancer Cell. 2005 Sep;8(3):241-54. doi:

Insert

ITGB1 (ITGB1 Human)

Expression

Mammalian

Mutation

V737N

Available Since

March 13, 2023

Availability

Academic Institutions and Nonprofits only

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pPICZc-HsACTG1*-thymosinB-8His

Plasmid

#111147

Purpose

Expresses human gamma-actin (codon is optimised for expression in Pichia pastoris) fused with thymosin beta and a His tag.

Depositor

Mohan Balasubramanian

Article

Hatano et al J Cell Sci. 2018 Mar 13. pii: jcs.213827

Insert

actin gamma 1 (ACTG1 Human)

Use

Pichia pastoris integration vector

Tags

Thymosin beta and a His-tag

Expression

Yeast

Mutation

codon-optimised for expression in Pichia pastoris

Promoter

AOX1

Available Since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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pLV-EF1a-DHB-mTurq2

Plasmid

#212667

Purpose

Express tagged DHB

Depositor

Yumi Konagaya, Tobias Meyer

Article

Konagaya et al Nature. 2024 Jul;631(8020):424-431. doi:

Insert

DHB (HELB Human)

Use

Lentiviral

Tags

mTurq2

Promoter

EF-1a

Available Since

Aug. 5, 2024

Availability

Academic Institutions and Nonprofits only

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TFORF1483

Plasmid

#143123

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

TP63 (TP63 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
TFORF1484

Plasmid

#143124

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

TP63 (TP63 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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TFORF1480

Plasmid

#142802

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

TP63 (TP63 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

June 8, 2023

Availability

Academic Institutions and Nonprofits only

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CL20_mEGFP_ASCC3_GRIK2

Plasmid

#205774

Purpose

Express mEGFP-tagged fusion protein, ASCC3_GRIK2 from patient-derived sequence

Depositor

Richard Kriwacki

Article

Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

Insert

ASCC3_GRIK2 (ASCC3, GRIK2 Human)

Tags

mEGFP

Expression

Mammalian

Available Since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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CuGFP-ATG8(416)

Plasmid

#49423

Purpose

Expresses ATG8 fused at the N terminus to GFP under the control of the CUP1 promoter.

Depositor

Daniel Klionsky

Article

Wang et al J Biol Chem. 2012 Nov 2;287(45):37964-72

Insert

autophagy-related 8 (ATG8 Budding Yeast)

Tags

GFP

Expression

Bacterial and Yeast

Promoter

CUP1

Available Since

Jan. 2, 2014

Availability

Academic Institutions and Nonprofits only
YCe3736_HC_Amp_ccdB_receiver vector

Plasmid

#100637

Purpose

EMMA toolkit receiver vector

Depositor

Yizhi Cai

Article

Martella et al ACS Synth Biol. 2017 Apr 24. doi: 10.102

Type

Empty backbone

Use

Synthetic Biology

Available Since

Nov. 17, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV5 Flag TGIF

Plasmid

#14047

Depositor

Joan Massague

Article

Wotton et al J Biol Chem. 1999 Dec 24. 274(52):37105-

Insert

TGIF (TGIF1 Human)

Tags

Flag

Expression

Mammalian

Available Since

May 10, 2007

Availability

Academic Institutions and Nonprofits only
mcherry-DN KASH

Plasmid

#125553

Purpose

mcherry labeled DN-KASH

Depositor

Daniel Conway

Article

Mayer et al Cell Mol Bioeng. 2019 Jul 11;12(4):289-3

Insert

SYNE1 (SYNE1 Human)

Tags

mcherry

Expression

Mammalian

Mutation

Truncated form: only the KASH domain of nesprin-…

Promoter

CMV

Available Since

July 25, 2019

Availability

Academic Institutions and Nonprofits only

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pHR-FRB:Akt2

Plasmid

#72899

Purpose

Recruitable Akt2 (LocaTOR2)

Depositor

Ivan Yudushkin

Article

Ebner et al J Cell Biol. 2017 Jan 31. pii: jcb.20161

Insert

Serine/threonine protein kinase Bβ (AKT2 Human)

Use

Lentiviral

Tags

FRB(T2098L)

Expression

Mammalian

Available Since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pCHNFI-X2

Plasmid

#31402

Depositor

Richard Gronostajski

Article

Chaudhry et al J Biol Chem. 1998 Jul 17. 273(29):18538-

Insert

Nuclear Factor IX isoform 2 (Nfix Mouse)

Tags

NA

Expression

Mammalian

Available Since

Sept. 21, 2011

Availability

Academic Institutions and Nonprofits only

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WT (DVD)

Plasmid

#184316

Purpose

To study dynamics of G-actin using NMR spectroscopy.

Depositor

Michael Rosen

Article

Ali et al Nat Struct Mol Biol. 2022 Apr;29(4):320-

Insert

Act5C (Act5C Fly)

Tags

Hexahistidine followed by B-thymosin

Expression

Yeast

Promoter

AOX1

Available Since

May 19, 2022

Availability

Academic Institutions and Nonprofits only

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pCK004 ubi:mCerulean_SV40 (#302)

Plasmid

#195978

Purpose

Expression construct

Depositor

Alexa Burger, Christian Mosimann

Article

Kemmler et al Development. 2023 Mar 28:dev.201531. doi

Insert

ubi:mCerulean

Use

Other

Available Since

Feb. 10, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-CaMKIIa-ChRmine-oScarlet-WPRE

Plasmid

#183518

Purpose

Optogenetics

Depositor

Karl Deisseroth

Article

Kishi et al Cell. 2022 Feb 17;185(4):672-689.e23. do

Insert

ChRmine-oScarlet

Use

AAV

Promoter

CaMKIIa

Available Since

May 13, 2022

Availability

Academic Institutions and Nonprofits only

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N1-mSin1.5-GFP

Plasmid

#72909

Purpose

GFP-tagged mSin1 (isoform 5)

Depositor

Ivan Yudushkin

Article

Ebner et al J Cell Biol. 2017 Jan 31. pii: jcb.20161

Insert

Target of rapamycin complex 2 subunit MAPKAP1, isoform 5 (MAPKAP1 Human)

Tags

eGFP

Expression

Mammalian

Available Since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pHR-TcRb:mCherry-FKBP

Plasmid

#72904

Purpose

ER-targeted mCherry-tagged recruiter construct

Depositor

Ivan Yudushkin

Article

Ebner et al J Cell Biol. 2017 Jan 31. pii: jcb.20161

Insert

T cell receptor β chain

Use

Lentiviral

Tags

biotinylation acceptor peptide (AP) and mCherry, …

Expression

Mammalian

Available Since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pMSCV LDTS-LIRmut

Plasmid

#189001

Purpose

lipophagy-inducing adaptor protein, negative control

Depositor

Atsushi Hoshino

Article

Minami et al Nat Commun. 2023 Jul 13;14(1):4084. doi:

Insert

LDTS-LIRmut

Use

Retroviral

Tags

HA

Available Since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
IF189

Bacterial Strain

#134836

Purpose

Rapid and efficient recombineering and purificiation of modified lambda phage DNA

Depositor

IIya Finkelstein

Article

Kim et al Sci Rep. 2017 May 18;7(1):2071. doi: 10.

Bacterial Resistance

None

Available Since

Jan. 21, 2020

Availability

Industry, Academic Institutions, and Nonprofits
YSBE 557 : pNAT_GFP

Plasmid

#92085

Purpose

Plasmid for tagging proteins in Cryptococcus neoformans; GFP tag, NAT selection

Depositor

Yong-Sun Bahn

Article

So et al J Microbiol Biotechnol. 2017 Feb 28;27(2

Insert

GFP

Use

tagging proteins in cryptococcus neoforman

Available Since

July 10, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pLKO.puro_shGLS

Plasmid

#110335

Purpose

shGLS (Target CAACTGGCCAAATTCAGTC), silence human GLS gene, puromycin selection

Depositor

Sandra Martha Gomes Dias

Article

Dias et al Oncogene. 2020 Jan;39(3):690-702. doi: 1

Insert

GLS glutaminase (GLS Human, Synthetic)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

U6 (RNA PolIII)

Available Since

Feb. 23, 2021

Availability

Academic Institutions and Nonprofits only

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cmv-sfGFP-GB1-scAB-ubc-nls-pcp-5xSunTag(SRv1-2P)

Plasmid

#185800

Purpose

one plasmid encoding scFv GFP and pcp-5xSunTag components for SunRISER SRv1

Depositor

Robin Lee

Article

Guo et al Cell Rep Methods. 2022 May 25;2(6):10022

Insert

cmv-sfGFP-GB1-scAB--ubc-nls-pcp-5xSunTag

Expression

Mammalian

Available Since

July 18, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge

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