We narrowed to 15,064 results for: pET
-
Plasmid#194661PurposeProtein production of circular permuted HaloTag (cpHaloTag154/156) in bacteriaDepositorInsertcpHaloTag154/156
UseTagsHis-tagExpressionBacterialMutationPromoterT7Available SinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
Pet28a-His-tag-BS2
Plasmid#220347PurposeE. coli expression vector to purify BS2DepositorInsertBS2
UseTagsHis tagExpressionBacterialMutationPromoterAvailable SinceJune 18, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET 15b-UbcH5a/2417
Plasmid#11472DepositorAvailable SinceApril 5, 2006AvailabilityAcademic Institutions and Nonprofits only -
pET22b-mEB1-eGFP-6xHis
Plasmid#130981PurposeBacterial expression of mouse EB1-GFP-6xHis for protein purification.DepositorAvailable SinceOct. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28_mouse-Caspase-11_enzymatic-domain
Plasmid#183382PurposeOverexpression of catalytic domain of inflammatory caspase in bacteriaDepositorInsertCaspase-11 (AA100-373) (Casp4 Mouse)
UseTagsHis-TEVExpressionBacterialMutationPromoterT7Available SinceJuly 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET16b-Rnl2(1-249)K227Q
Plasmid#14072DepositorInsertRnl2(1-249)
UseTagsHis (10x)ExpressionBacterialMutationchanged Lys227 to Glutamine Gly112 is actually C…PromoterAvailable SinceFeb. 22, 2007AvailabilityAcademic Institutions and Nonprofits only -
[pSK104] pET28 SLC38A9N-His
Plasmid#136142Purposebacterial expression of SLC38A9 N term fragment (1-119)DepositorInsertSLC38A9 N term fragment (1-119) (SLC38A9 Human)
UseTagsHisExpressionBacterialMutationPromoterAvailable SinceApril 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28a(+)-SpdCas9-SBiT_Strep-Tag-II
Plasmid#198750PurposeExpresses S. Pyogenes dCas9 fused to SmallBiT fragment of split NanoLuc luciferaseDepositorInsertSpdCas9 - SmallBiT
UseTagsStrep-tag IIExpressionBacterialMutationD10A, H840A (nuclease inactivating mutations in &…PromoterT7Available SinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28a(+)-SpdCas9-LBiT_Strep-Tag-II
Plasmid#198751PurposeExpresses S. Pyogenes dCas9 fused to LargeBiT fragment of split NanoLuc luciferaseDepositorInsertSpdCas9 - LargeBiT
UseTagsStrep-tag IIExpressionBacterialMutationD10A, H840A (nuclease inactivating mutations in &…PromoterT7Available SinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pF709-pET28a-Hs-FL-ETFbeta-NHis wt
Plasmid#85110Purposeexpression of recombinant human full-length ETFbeta wt with N-terminal 6xHis-tagDepositorInserthuman full-length ETFbeta (ETFB Human)
UseTags6xHisExpressionBacterialMutationPromoterT7Available SinceNov. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET16b-Trx CC32/35SS
Plasmid#36946DepositorInsertThioredoxin 1 (TXN Human)
UseTagsHis tagExpressionBacterialMutationboth active site cysteines (32,35) mutated to ser…PromoterT7Available SinceMay 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET-A3A(N57Q)-BE3
Plasmid#158157PurposeT7 promoter bacterial expression plasmid for hAPOBEC3A(N57Q)-BE3 with N-terminal 6xHis-tag and C-terminal SV40 NLSDepositorInsert6xHis-hA3A(N57Q)-XTEN linker-hSpCas9n(D10A)-UGI-SV40NLS
UseTags6xHis tag on N-terminusExpressionBacterialMutationPromoterT7Available SinceNov. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET_SZ1-RGG-GFP-RGG
Plasmid#124937PurposeExpression of SZ1-RGG-GFP-RGGDepositorInsertSYNZIP1-RGG-GFP-RGG (using LAF-1 RGG domain) (laf-1 Nematode)
UseTagsHisExpressionBacterialMutationPromoterAvailable SinceMay 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET30b-5M SrtA with TEV site
Plasmid#86962PurposeExpresses calcium-dependent Sortase A with a TEV cleavage site proximal to the C-terminal histidine tagDepositorInsertS.aureus SrtA 5M
UseTags6x Histidine tag and TEV siteExpressionBacterialMutationP94R, D160N, D165A, K190E, K196TPromoterT7Available SinceMay 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET-Octo_VirD2_NLS-Cas9-6xHis
Plasmid#232296PurposeExpresses Cas9 protein fused with octopine-type VirD2-originating NLSDepositorInsertOcto_VirD2_NLS-Cas9-6xHis
UseCRISPRTags6xHis and Octopine-type VirD2-derived NLSExpressionBacterialMutationPromoterT7Available SinceMarch 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
3521 pET15b Bid p15
Plasmid#8782DepositorAvailable SinceJuly 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
pET28a(+) 6xHis-3xFlag-IFIT5
Plasmid#53561Purposebacterial expression of IFIT5DepositorInsertIFIT5 (IFIT5 Human)
UseTags3xFLAG, 6xHIS, and TEV siteExpressionBacterialMutationPromoterT7Available SinceMay 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET51b(+)_LAMA-F98 (for GFP)
Plasmid#130714PurposePlasmid encoding the bacterial expression of LAMA-F98 for GFPDepositorInsertLAMA-F98
UseTagsHisx10ExpressionBacterialMutationDeleted first Met, Ala and Last Ser from the Enha…PromoterAvailable SinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28-Nsp14-TEV-6His
Plasmid#154952PurposeExpresses Nsp14 from SARS-CoV-2 with a C-terminal TEV-protease cleavable 6His tag in E. coliDepositorInsertNsp14-TEV-6His (ORF1ab SARS-CoV-2)
UseTagsTEV-6HisExpressionBacterialMutationPromoterT7 PromoterAvailable SinceAug. 5, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET21b-Caspase-3(D3A,V266E)
Plasmid#90091PurposeExpresses caspase D3A and V266E mutant in bacterial cellsDepositorInsertCaspase 3 (CASP3 Human)
UseTagsHis tagExpressionBacterialMutationD175A D28A D9A V266EPromoterAvailable SinceMay 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pETM11-6xHis-TEV-hORF1p_K3AK4A
Plasmid#202585PurposeAllows for IPTG-inducible expression of the human L1RP ORF1 protein with the K3A and K4A mutations in bacteria with an N-terminal 6xHis tag and TEV cleavage site for purification with a minimal scarDepositorInsertORF1p
UseTags6x His and TEV protease cleavage siteExpressionBacterialMutationChanged ORF1p Lysine 3 to Alanine, changed ORF1p …PromoterAvailable SinceNov. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28-his6-HA3-SNAPf
Plasmid#188941PurposeT7 expression vector for making his6- and HA3-tagged SNAP proteinDepositorInserthis6-HA3-SNAPf
UseTagsExpressionBacterialMutationPromoterAvailable SinceSept. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SUMO-AsCas12f-2NLS
Plasmid#171613PurposeNLS-AsCas12f1-NLS heterologous expression plasmid for RNPDepositorInsertNLS-AsCas12f1-NLS
UseTagsExpressionBacterialMutationPromoterAvailable SinceSept. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28_6xHis-PARP1-CAT-L713F
Plasmid#173944PurposeBacterial expression of isolated PARP1 CAT domain containing L713F gain-of-function mutationDepositorInsertPARP1-CAT-L713F (PARP1 Human)
UseTags6xHisExpressionBacterialMutationL713F, V762APromoterAvailable SinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET23b-Casp2-Delta Card
Plasmid#11810DepositorInsertCaspase-2 Delta Card (CASP2 Human)
UseTagsHisExpressionBacterialMutationDelta CardPromoterAvailable SinceMay 12, 2006AvailabilityAcademic Institutions and Nonprofits only -
pet28a-ClbB-NRPS-NHis
Plasmid#49217PurposeNRPS module of ClbB containing CAT domains with N-terminal 6His tagDepositorInsertClbB-NRPS
UseTags6x His tag and T7 tagExpressionBacterialMutationcontains aa4-1080 of reference sequence NP_754362…PromoterT7Available SinceMarch 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET22-His-TEV-DOT1L
Plasmid#89610PurposeBacterial expression vector for an N-terminally His-tagged (TEV-cleavable) version of human Dot1L residues 1-416DepositorAvailable SinceAug. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET28a(+) 6xHis-TEV-IFIT1
Plasmid#53557Purposebacterial expression of IFIT1DepositorAvailable SinceMay 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET22b-mEB1-mCherry-6xHis
Plasmid#130984PurposeBacterial expression of mouse EB1-mCherry-6xHis for protein purification.DepositorInsertEB1-mCherry-6xHis (Mapre1 Mouse)
UseTagsmCherry-6xHisExpressionBacterialMutationPromoterT7Available SinceOct. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
VV020: WT Arch-3 - eGFP in pET28b
Plasmid#58488PurposeExpresses WT Archaerhodopsin-3 fused to eGFP (with 6x C-terminal His tag) in E. coliDepositorInsertArchaerhodopsin-3
UseTagseGFPExpressionBacterialMutationPromoterT7Available SinceAug. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET-45b-HP1a-mCherry
Plasmid#185012PurposeBacterial Expression of 6xHis CBX5 (HP1a) mCherry Fusion ProteinDepositorAvailable SinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SENP6 (catalytic domain)
Plasmid#16359DepositorInserthuman SENP6 catalytic domain (SENP6 Human)
UseTagsHisExpressionBacterialMutationAmino Acid 628 to 1112 catalytic domain of human …PromoterAvailable SinceDec. 19, 2007AvailabilityAcademic Institutions and Nonprofits only -
His-SUMO-flag-hDDI2_pET11a
Plasmid#169121Purposeexpression of proteinDepositorAvailable SinceMay 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28-MBP-TEV-AICD
Plasmid#69927Purposebacterial expression of MBP-TEV-AICDDepositorAvailable SinceOct. 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHS0663 pET45b(+) KraIscB-1 TwinStrep
Plasmid#176536PurposeBacterial expression of KraIscB-1-TwinStrepDepositorInsertKraIscB-1
UseTagsTwinStrepExpressionBacterialMutationPromoterAvailable SinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET24a HAB1 (172-511)
Plasmid#177844PurposeBacterial Expression of HAB1DepositorInsertProtein phosphatase 2C 16
UseTags6xHis-GSTExpressionBacterialMutationPromoterAvailable SinceDec. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
PET-B-eSpCas9-NLS-6xHis
Plasmid#207383PurposeExpression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cellsDepositorInsertB-eSpCas9
UseCRISPRTags6xHisExpressionBacterialMutationK848A, K1003A, R1060A, amino acids 1005-1013 repl…PromoterT7Available SinceOct. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SENP8 (full length))
Plasmid#16361DepositorInserthuman SENP8 full length (SENP8 Human)
UseTagsHisExpressionBacterialMutationhuman SENP8 full length, see Depositor CommentsPromoterAvailable SinceDec. 19, 2007AvailabilityAcademic Institutions and Nonprofits only -
pET27b-ecDHFR-N23PP/L28F
Plasmid#118584PurposeBacterial expression of E.coli DHFR N23PP and L28F mutantDepositorInsertDHFR
UseTagsExpressionBacterialMutationL28 mutated to F28, N23 mutated to P23, a proline…PromoterT7Available SinceNov. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SdrG_N2N3-HIS-ybbr
Plasmid#101238PurposeSdrG N2 and N3 domains with C-terminal ybbr tag for covalent immobilizationDepositorInsertSdrG_N2N3
UseTagsH3V 3C, HIS, and ybbrExpressionBacterialMutationPromoterT7Available SinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28-His6-Ubc9-K14R
Plasmid#185759PurposeBacterial expression of His6-Ubc9-K14RDepositorInsertSUMO-conjugating enzyme UBC9 (UBE2I Human)
UseTagsThrombin-cleavable His6-tagExpressionBacterialMutationK14RPromoterT7Available SinceJuly 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pETDuet RagA(D130N)-RagC
Plasmid#99642PurposeBacterial expression of RagA(D130N)-RagCDepositorUseTagsHis tagExpressionBacterialMutationD130NPromoterAvailable SinceNov. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pETDuet RagA(T21N)-RagC
Plasmid#99644PurposeBacterial expression of RagA(T21N)-RagCDepositorUseTagsHis tagExpressionBacterialMutationT21NPromoterAvailable SinceNov. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET28a-TGFα-GSY-SPM
Plasmid#163615PurposeExpresses mature human Transforming Growth Factor alpha fused to SPM in bacterial cells for calcium-induced covalent coupling to Epidermal Growth Factor Receptor.DepositorInsertTGFa-GSY-SPM (TGFA Synthetic, Human)
UseTagsC-tag and His6ExpressionBacterialMutationPromoterT7Available SinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
PET28a-cpLumiLuc-Rapamycin sensor
Plasmid#186292PurposeExpression Rapamycin sensor in e coliDepositorInsertcpLumiLuc-Rapamycin sensor
UseTagsHisTagExpressionBacterialMutationPromoterAvailable SinceAug. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-KIX (586-672)
Plasmid#99336PurposeBacterial expression of mouse CBP KIX domainDepositorAvailable SinceSept. 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TRE-YPet-WPRE
Plasmid#193342PurposeAAV-mediated expression of YPet under the TRE promoter in mammalian cellsDepositorInsertYPet
UseAAVTagsExpressionMammalianMutationPromoterTREAvailable SinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET-FLAG-SpCas9-HF1
Plasmid#126770PurposeExpression of increased fidelity SpCas9-HF1 in bacterial cellsDepositorInsertSpCas9-HF1
UseCRISPRTags3xFLAG, 6xHis, MBP, and NLSExpressionBacterialMutationN497A, R661A, Q695A, Q926APromoterT7Available SinceMarch 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET23-PBD(65-150)-N-Cys
Plasmid#13722DepositorInsertPBD(65-150)-N-Cys (PAK1 Human)
UseTags6xHisExpressionBacterialMutationMet-Cys-Gly-PBD(65-150)-PromoterAvailable SinceMarch 28, 2007AvailabilityAcademic Institutions and Nonprofits only -
pET28-6His-SUMO-Nsp14
Plasmid#154956PurposeExpresses Nsp14 from SARS-CoV-2 with a N-terminal SUMO-protease cleavable 6His-SUMO tag in E. coliDepositorInsert6His-SUMO-Nsp14 (ORF1ab SARS-CoV-2)
UseTags6His-Thrombin-SUMOExpressionBacterialMutationPromoterT7 PromoterAvailable SinceAug. 5, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits