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Showing: 2801 - 2840 of 160228 results

  2. pcDNA3.1-CRISPR-Puro

    Plasmid
    #208646
    Purpose
    pcDNA3.1 based empty vector for transient transfection of sgRNA-cas9. U6 promoter ~ puro Res cloned from lentiCRISPRv2 (Addgene#52961), lenti virus sequences not included.
    Depositor
    Quanfu Ma
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Clawed frog nucleoplasmin NLS, Flag, Puromycin re…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV and U6 in tandem
    Available since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208646-plasmid-map-sequence-id-418362

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  4. BK Dunlop

    Plasmid
    #25466
    Depositor
    Peter Howley
    Insert
    human papovavirus BK Dunlop
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Sept. 26, 2011
    Availability
    Academic Institutions and Nonprofits only

  10. Lenti-AsCpf1-Blast

    Plasmid
    #84750
    Purpose
    Expresses human codon-optimized AsCpf1 protein and blasticidin resistance from EFS promoter. Lentiviral backbone.
    Depositor
    Hyongbum Kim
    Article
    Kim et al Nat Methods. 2016 Dec 19. doi: 10.1038/n
    Insert
    AsCpf1
    Use
    CRISPR and Lentiviral
    Tags
    NLS-3xHA (C terminal on insert)
    Expression
    Mammalian
    Mutation
    Promoter
    EFS-NS
    Available since
    Dec. 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    84750-plasmid-map-sequence-id-164466

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  21. AAV phSyn1-FSF-FLEX-ChR2(H134R)-EYFP-WPRE-bGHpA

    Plasmid
    #65454
    Purpose
    Can be used to generate AAV virus that will express the ChR2(H134R)-EYFP channelrhodopsin fusion protein in neurons under intersectional control by Flp and Cre recombinases
    Depositor
    Hongkui Zeng
    Article
    Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10
    Insert
    ChR2(H134R)-EYFP
    Use
    AAV
    Tags
    EYFP
    Expression
    Mutation
    H134R variant
    Promoter
    hSyn1
    Available since
    July 7, 2015
    Availability
    Academic Institutions and Nonprofits only
    65454-plasmid-map-sequence-id-436383

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  22. pT3TS-zSpG

    Plasmid
    #179316
    Purpose
    In vitro transcription of SpG-Cas9 mRNA zebrafish codon-optimized from T3 promoter
    Depositor
    Miguel Angel Moreno-Mateos
    Article
    Vicencio et al Nat Commun. 2022 May 12;13(1):2601. doi:
    Insert
    Zebrafish codon-optimized Cas9 variant SpG
    Use
    CRISPR; In vitro transcription by t3 promoter
    Tags
    SV40-NLS
    Expression
    Mutation
    SpG=D1135L/S1136W/G1218K/E1219Q/ R1335Q/T1337R
    Promoter
    Available since
    May 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    179316-plasmid-map-sequence-id-354720

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  26. pExp-sfGFP

    Plasmid
    #112573
    Purpose
    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and sfGFP (superfolder green fluorescent protein) fusions with optional C-terminal StrepII-tag
    Depositor
    Marko Hyvönen
    Type
    Empty backbone
    Use
    Tags
    8xHis, Strep II, and sfGFP
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    112573-plasmid-map-sequence-id-217227

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  29. TFORF0724

    Plasmid
    #143070
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    NFKB2 (NFKB2 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    June 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    143070-plasmid-map-sequence-id-398863

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  36. TFORF0061

    Plasmid
    #141502
    Purpose
    Lentiviral vector for overexpressing the CTBP2 transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    CTBP2 (CTBP2 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    141502-plasmid-map-sequence-id-276642

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  40. pGEX-2T-Rac1-Q61L

    Plasmid
    #12979
    Depositor
    Gary Bokoch
    Insert
    Rac1 constitutively active (RAC1 Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    Q61L Constitutively active
    Promoter
    Available since
    Oct. 27, 2006
    Availability
    Academic Institutions and Nonprofits only
Showing: 2801 - 2840 of 160228 results