165,839 results
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Plasmid#234070PurposeFor lentiviral expression of user-specified pegRNA in tandem with epegRNA (ATP1A1-T804N) for ouabain co-selectionDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available SinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits only
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AAV-shRNA-ctrl
Plasmid#85741PurposeshRNA ctrlDepositorInsertshRNA ctrl
UseAAVTagsEYFPExpressionMutationPromoterAvailable SinceApril 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pACAGW-ChR2-Venus-AAV (AAV9)
Viral Prep#20071-AAV9PurposeReady-to-use AAV9 particles produced from pACAGW-ChR2-Venus-AAV (#20071). In addition to the viral particles, you will also receive purified pACAGW-ChR2-Venus-AAV plasmid DNA. CAG-driven, channelrhodopsin fused to Venus for optogenetic activation. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCAGGTagsVenusAvailable SinceMarch 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
Cas9-GFP_sg_mAMPKa2
Plasmid#79005PurposeExpresses WT Cas9 and GFP, along with a sgRNA to mouse AMPK alpha 2.DepositorAvailable SinceJune 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCMV-eNme2-C-ABE8e
Plasmid#185667PurposeExpresses eNme2-C-ABE8e in mammalian cellsDepositorInsertecTadA(8e)-neNme2-C
UseCRISPRTagsExpressionMammalianMutationNme2Cas9 P6S/D16A/E33G/K104T/D152A/F260L/A263T/A3…PromoterAvailable SinceSept. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
EGFP-E-Syt2
Plasmid#66831PurposeMammalian Expression, Bacterial ExpressionDepositorAvailable SinceJune 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGL3-SEC61B.N-BSD.P2A.miniIAA7.3xFlag
Plasmid#216250PurposeHDR template to tag endogenous human SEC61B N-terminus with BSD.P2A.miniIAA7.3xFlagDepositorInsertHDR template for human SEC61B (SEC61B Human, HDR template, Synthetic)
UseCRISPR; Hdr templateTagsBSD.P2A.miniIAA7.3xFlagExpressionMammalianMutationPromoterAvailable SinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
AMPK(T>A)-SPARK
Plasmid#226256Purposephospho-null mutant of AMPK-SPARKDepositorInsertAMPK(T>A)-SPARK
UseTagsExpressionMammalianMutationsubstituted Threonine 20 with AlaninePromoterAvailable SinceOct. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCaggs-mGold2t
Plasmid#231764PurposeExpression of mGold2t in mammalian cellsDepositorInsertmGold2t
UseTagsExpressionMammalianMutationmGold2t is mGold with V1A;V22I;H77N;Q80R;K101E;D1…PromoterActin promoter with CMV enhancerAvailable SinceApril 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-mBATF
Plasmid#34575DepositorAvailable SinceDec. 16, 2011AvailabilityAcademic Institutions and Nonprofits only -
pKM496 (deletions) Zeo
Plasmid#109301PurposeORBIT integrating plasmid for deletion of target gene, using zeocin as a selection marker.DepositorInsertBxb1 attB site
UseOrbit integrating plasmidTagsExpressionMutationPromoterAvailable SinceMay 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3puro-EGFP-GAPDH
Plasmid#225475PurposeExpress mEGFP-fusion protein of GAPDHDepositorAvailable SinceOct. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAG1159 TOM20-ECFP-FIREmate
Plasmid#196693PurposeExpresses TOM20-ECFP-FIREmate in mammalian cellsDepositorInsertTOM20-ECFP-FIREmate
UseTagsFIREmateExpressionMammalianMutationPromoterAvailable SinceSept. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-FLEX-EGFPL10a (AAV5)
Viral Prep#98747-AAV5PurposeReady-to-use AAV5 particles produced from pAAV-FLEX-EGFPL10a (#98747). In addition to the viral particles, you will also receive purified pAAV-FLEX-EGFPL10a plasmid DNA. Cre-dependent EGFPL10a (EGFP-tagged ribosomal protein) expression for translating ribosome affinity purification (TRAP). These AAV preparations are suitable purity for injection into animals.DepositorPromoterEF1aTagsEGFP (Cre-dependent)Available SinceSept. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 RLUC POLIRES FLUC
Plasmid#45642PurposeBicistronic reporter plasmid expressing Renilla luciferase and firefly luciferase in mammalian cells.DepositorInsertRLUC - IRES - FLUC
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable SinceJune 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
GFP-polyQ74-NLS
Plasmid#231892PurposeForms nuclear HTT partial exon 1 Q74-NLS aggregatesDepositorInsertHTT partial exon 1 Q74-NLS (HTT Human)
UseTagsEGFP and SV40 NLSExpressionMammalianMutationCAG repeat expansionPromoterAvailable SinceFeb. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSH-CAG-AtAFB2.F74A-mCherry-IRES-puro
Plasmid#216244PurposeExpress AtAFB2.F74A mutant with mCherry tag for AID2DepositorInsertAtAFB2.F74A-mCherry
UseCRISPR, TALEN, and Unspecified ; Human safe harbo…TagsmCherryExpressionMammalianMutationF74A mutationPromoterCAGAvailable SinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-TadCBEd-SpRY-P2A-EGFP (BKS428)
Plasmid#223125PurposepCMV and pT7 Human expression plasmid for TadCBEd-SpRY with C-term BPNLS-P2A-EGFPDepositorInserthuman codon optimized TadCBEd-SpRY-2xUGI-P2A-EGFP
UseCRISPRTags2xUGI-BPNLS-P2A-EGFP and BPNLS-TadA-CDdExpressionMammalianMutationTadA-CDd mutations; nSpRY=D10A/A61R/L1111R/D1135L…PromoterCMV and T7Available SinceApril 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHEN2-SG4 R105A
Plasmid#196072PurposeExpress mutant (R105A) SG4 in E.coliDepositorInsertSG4 R105A
UseTags3XFLAG and 6XHisExpressionBacterialMutationR105A mutation in SG4PromoterT7Available SinceMarch 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMK345 (Hygro-P2A-mAID-mClover)
Plasmid#121179PurposeN-terminal taggingDepositorInsertHygro-P2A-mAID-mClover
UseCRISPRTagsExpressionMutationPromoterAvailable SinceFeb. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28:GFP
Plasmid#60733PurposepET28 driving expression of GFP, biobrick part Bba_E0040DepositorInsertGFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable SinceNov. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FLAG-KD LKB1
Plasmid#8591DepositorInsertLKB1 (STK11 Human)
UseTagsFLAGExpressionMammalianMutationK78I: lysine 78 mutated to isoleucine; kinase deadPromoterAvailable SinceJuly 18, 2005AvailabilityAcademic Institutions and Nonprofits only -
AiP12918 - pAAV-AiE0459m_3xC2-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2918)
Plasmid#224016PurposeAiE0459m_3xC2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in cortical L5_ET neuronsInsertSYFP2
UseAAVTagsExpressionMutationNAPromoterminBGAvailable SinceOct. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pORANGE Cloning template vector
Plasmid#131471PurposeCloning template form ORANGE method based knock-in constructsDepositorTypeEmpty backboneUseTagsHAExpressionMammalianMutationPromoterU6 and CbhAvailable SinceSept. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
GluA2 (px330:2-guideCas9)
Plasmid#169420PurposeExpression of Cas9 and 2 guidesDepositorInsertspCas9
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable SinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pME-Cas9-T2A-GFP
Plasmid#63155PurposeMiddle Entry clone for Gateway containing a zebrafish codon-optimized Cas9 flanked by 2 NLS and followed by inframe GFP. Cas9 and GFP are separated by the sequence of a T2A self-cleaving peptide.DepositorInsertCas9-T2A-GFP
UseCRISPR; Tol2 middle entry vector for gatewayTagsExpressionMutationPromoterAvailable SinceApril 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSFFV_sfCherry3C(1-10)
Plasmid#117482PurposeLentiviral vector for constitutive expression of sfCherry3C(1-10)DepositorInsertsfCherry3C(1-10)
UseLentiviralTagsExpressionMammalianMutation5 substitutions (K45R, G52D, T106A, K182R, N194D)…PromoterSFFVAvailable SinceJuly 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
p177bp-LacOx50-Hyg
Plasmid#210213PurposeIntegrates 50xLacO repeats with a hygromycin drug selection marker into 177bp repeat region.DepositorInsert50xLacO repeats derived from: PMID: 12864855.
UseTagsExpressionMutationPromoterAvailable SinceNov. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBXNH3CA_MBP
Plasmid#132700Purposeexpresses Avi-tagged MBP in E.coli, to use as positive control in ELISADepositorInsertMaltose binding Protein
UseTagsAvi and HisExpressionBacterialMutationPromoterpBADAvailable SinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRK5-myc-Cdc42-Q61L
Plasmid#12974DepositorInsertCdc42 constitutively active (CDC42 Human)
UseTagsMycExpressionMammalianMutationQ61L Constitutively activePromoterAvailable SinceMarch 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
ANO6-Plvx-mCherry-c1
Plasmid#62554PurposeFull length mouse ANO6 tagged with mCherry on the C-terminusDepositorAvailable SinceMarch 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
mCherry-Rab5DN(S34N)
Plasmid#35139DepositorInsertRab5a (RAB5A Human)
UseTagsmCherryExpressionMammalianMutationDominant Negative- S34NPromoterCMVAvailable SinceMarch 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
pLIB
Plasmid#80610PurposepLIB - Library vector (biGBac system for expression of protein complexes in insect cells)DepositorTypeEmpty backboneUseTagsExpressionInsectMutationPromoterpolyhedrinAvailable SinceOct. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
iNos-eGFP
Plasmid#207251PurposeReporter for expression of eGFP under control of iNos promoterDepositorAvailable SinceJuly 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEG302_22aa_SunTag_MQ1(Q147L)_g4+g10+g18
Plasmid#172318PurposeCRISPR dCas9 SunTag system to target a variant of bacterial DNA methyltransferase MQ1 to install CG specific methylation to the FWA locus with three guide RNAsDepositorInsertg18_U6_g10_U6_g4_U6_NOS_MQ1(Q147L)_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_3xNLS_linker_10xGCN4_OCS
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable SinceSept. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
MCS-13X Linker-BioID2-HA
Plasmid#80899PurposeTo fuse your protein of interest to the N-terminus of BioID2 and use in proximity-dependent biotin identification (BioID); MCS and a flexible 25nm GS linker are present upstream of BioID2 and HA tags.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseTagsHA and humanized A. aeolicus BioID2 with R40GExpressionMammalianMutationPromoterCMVAvailable SinceOct. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pOSIP-KO (KanR, 186)
Plasmid#45985DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable SinceJuly 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
gag-P3-pol
Plasmid#211374PurposePackaging plasmid for v3b PE-eVLP productionDepositorInsertMMLVgag-P3-pol
UseTagsExpressionMammalianMutationPromoterAvailable SinceJan. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCdrA-gfp(ASV)C
Plasmid#111615PurposeFluorescence-based gauging of the level of the nucleotide secondary messenger cyclic di-GMP in Pseudomonas aeruginosa and related speciesDepositorInsertPcdrA-RBSII-gfp(ASV)-T0-T1
UseTagsExpressionBacterialMutationPromoterAvailable SinceJune 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-N-Flag-Caspase-1
Plasmid#75128PurposeExpresses N-terminal Flag-tagged Caspase-1 in mammalian cellsDepositorInsertcaspase 1 (Casp1 Mouse)
UseTagsFlagExpressionMammalianMutationP341S in Caspase-1 (please see depositor comments…PromoterCMVAvailable SinceMay 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMK349 (BSD-P2A-mAID-mCherry)
Plasmid#121183PurposeN-terminal taggingDepositorInsertBSD-P2A-mAID-mCherry
UseCRISPRTagsExpressionMutationPromoterAvailable SinceFeb. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-GFP (AAV5)
Viral Prep#37825-AAV5PurposeReady-to-use AAV5 particles produced from pAAV-CAG-GFP (#37825). In addition to the viral particles, you will also receive purified pAAV-CAG-GFP plasmid DNA. CAG-driven GFP expression. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCAGTagsGFPAvailable SinceOct. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
AAV-GfaABC1D-Cre-4x6T
Plasmid#196410PurposeAstrocytic expression of Cre recombinase in AAV; astrocyte specificity enhanced with 4x6T miRNA targeting cassetteDepositorHas ServiceAAV5InsertCre
UseAAV; Astrocyte-selectiveTagsExpressionMammalianMutationPromoterCAG and GfaABC1DAvailable SinceFeb. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-dCry-V5His
Plasmid#136241PurposeProtein expression of D. melanogaster (Dm) cytochrome with V5 and His tagsDepositorInsertDmCry
UseTagsHis and V5ExpressionMammalianMutationPromoterAvailable SinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
PrimeRoot.v2C-Cre
Plasmid#201739PurposeFor DNA insertion test in protoplastDepositorInsertePPE,Cre
UseCRISPRTagsExpressionPlantMutationPromoterAvailable SinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCI-neo-sOVA
Plasmid#25098DepositorInsertsoluble ovalbumin
UseTagsExpressionBacterial and MammalianMutationPromoterAvailable SinceJuly 1, 2010AvailabilityAcademic Institutions and Nonprofits only -
Mucolipin1-pHcRed C1
Plasmid#62959Purposefull length human Mucolipin-1 cloned into pHcRed1 C1DepositorInsertMucolipin-1 (MCOLN1 Human)
UseTagsHcRed1ExpressionMammalianMutationMucolipin-1 has 1 silent mutation compared to the…PromoterCMVAvailable SinceMay 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
PSD95-TS:YFP
Plasmid#42225DepositorInsertPSD95
UseTagsTS:YFPExpressionMammalianMutationPromoterAvailable SinceFeb. 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pmCherry-N1-cofilin
Plasmid#186750PurposeExpresses wildtype mCherry-tagged human cofilin in mammalian cellsDepositorAvailable SinceMarch 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
Mucolipin1 D471-472K-pHcRed C1
Plasmid#62961Purposefull length human Mucolipin-1 D471/472K cloned into pHcRed1 C1DepositorInsertMucolipin-1 (MCOLN1 Human)
UseTagsHcRedExpressionMammalianMutationD471/472K (and two silent mutations T77 ACA inst…PromoterCMVAvailable SinceMay 28, 2015AvailabilityAcademic Institutions and Nonprofits only