We narrowed to 21,826 results for: LEA

1. pmCer-TopBP1

   Plasmid

   #211412

   Purpose

   mammalian expression of human TopBP1 N-terminally fused to mCerulean

   Depositor

   Helmut Pospiech

   Insert

   TopBP1 (TOPBP1 Human)
   

   Tags

   mCerulean

   Expression

   Mammalian

   Available Since

   Jan. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pmCer-RecQL4

   Plasmid

   #211400

   Purpose

   mammalian expression of human RecQL4 N-terminally fused to mCerulean

   Depositor

   Helmut Pospiech

   Insert

   RecQL4 (RECQL4 Human)
   

   Tags

   mCerulean

   Expression

   Mammalian

   Available Since

   Jan. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLV-EGFP-Cre

   Plasmid

   #86805

   Purpose

   Lentiviral vector that expresses an EGFP-Cre fusion protein with a nuclear localization signal from the CMV promoter

   Depositor

   Amy Lasek

   Article

   Truitt et al eNeuro. 2016 Oct 31;3(5). pii: ENEURO.02

   Insert

   Cre recombinase
   

   Use

   Cre/Lox and Lentiviral

   Tags

   EGFP and nuclear localization signal: PKKKRKV

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLV sgRNA-Notch1 #1 GFP

   Plasmid

   #106950

   Purpose

   Lentivirus encoding sgRNA targeting murine Notch1. Includes GFP marker.

   Depositor

   Albert Edge

   Article

   Lenz et al Front Cell Dev Biol. 2019 Feb 19;7:14. d

   Insert

   gRNA targeting Notch1 (Notch1 Mouse)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Dec. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. AAV-CAGGS-EGFP

   Plasmid

   #22212

   Purpose

   AAV zinc finger donor vector for genomic targeting

   Depositor

   Rudolf Jaenisch

   Article

   Hockemeyer et al Nat Biotechnol. 2009 Sep;27(9):851-7. do

   Insert

   CAGGS-eGFP
   

   Use

   Zfn donor

   Available Since

   Oct. 14, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHLmMBP-8

   Plasmid

   #72347

   Purpose

   Mammalian expression of cytoplasmic mMBP TEV cleavage site-linked fusion proteins with C-terminal 6His-tag/Strep-tag II/HA-tag

   Depositor

   Luca Jovine

   Article

   Bokhove et al J Struct Biol. 2016 Feb 3. 194:1-7. pii

   Type

   Empty backbone

   Tags

   6His-tag, HA-tag, Strep-tag II, and mMBP with C-t…

   Expression

   Mammalian

   Promoter

   CMV enhancer + chicken beta-actin promoter

   Available Since

   Feb. 17, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHLmMBP-11

   Plasmid

   #72349

   Purpose

   Mammalian expression of secreted mMBP TEV cleavage site-linked fusion proteins with C-terminal 6His-tag/Strep-tag II/HA-tag

   Depositor

   Luca Jovine

   Article

   Bokhove et al J Struct Biol. 2016 Feb 3. 194:1-7. pii

   Type

   Empty backbone

   Tags

   6His-tag, HA-tag, Strep-tag II, and mMBP with C-t…

   Expression

   Mammalian

   Promoter

   CMV enhancer + chicken beta-actin promoter

   Available Since

   Feb. 17, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEIGW Grx1-roGFP2

   Plasmid

   #64990

   Purpose

   Mammalian expression of cytosolic Grx1-roGFP2 (lentiviral vector)

   Depositor

   Tobias Dick

   Article

   Gutscher et al Nat Methods. 2008 Jun;5(6):553-9. doi: 1

   Insert

   Glutaredoxin-1 (GLRX Human)
   

   Use

   Lentiviral

   Tags

   roGFP2

   Expression

   Mammalian

   Available Since

   July 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCMVTNT-T7-KPNA2

   Plasmid

   #26678

   Depositor

   Bryce Paschal

   Article

   Kelley et al BMC Cell Biol. 2010 Aug 11;11:63. doi: 1

   Insert

   Karyopherin Alpha 2 (KPNA2 Human)
   

   Tags

   T7 (MASMTGGQQMG)

   Expression

   Mammalian

   Available Since

   Nov. 10, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEJS578_DD-dSpyCas9-mCherry-APEX2

    Plasmid

    #108570

    Purpose

    Expression of drug-inducible dSpyCas9-mCherry-APEX2 for biotin-labeling genomic element-associated protein factors

    Depositor

    Erik Sontheimer

    Article

    Gao et al Nat Methods. 2018 Jun;15(6):433-436. doi

    Insert

    dSpyCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    APEX2, Destabilization domains, Flag, and mCherry…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCS2+ EB3-mKate2

    Plasmid

    #105940

    Purpose

    in vitro transcription

    Depositor

    Caren Norden

    Article

    Strzyz et al Dev Cell. 2015 Jan 26;32(2):203-19. doi:

    Insert

    EB3 (MAPRE3 Human)
    

    Tags

    mKate2

    Expression

    Bacterial

    Available Since

    May 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pFLAG-Tetherin

    Plasmid

    #41070

    Depositor

    Paul Spearman

    Article

    Hammonds et al J Virol. 2012 Feb;86(4):2259-72. Epub 20

    Insert

    Tetherin (BST2 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 30, 2012

    Availability

    Academic Institutions and Nonprofits only

13. pQE-60 Grx1-roGFP2-His

    Plasmid

    #64799

    Purpose

    Bacterial expression of His-tagged Grx1-roGFP2

    Depositor

    Tobias Dick

    Article

    Gutscher et al Nat Methods. 2008 Jun;5(6):553-9. doi: 1

    Insert

    Glutaredoxin-1 (GLRX Human)
    

    Tags

    His and roGFP2

    Expression

    Bacterial

    Available Since

    Aug. 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. AAVS1 SA-2A-puro-pA donor

    Plasmid

    #22075

    Purpose

    AAV zinc finger donor vector for genomic targeting

    Depositor

    Rudolf Jaenisch

    Article

    Hockemeyer et al Nat Biotechnol. 2009 Sep;27(9):851-7. do

    Insert

    SA-Puro
    

    Use

    Zfn donor

    Available Since

    Oct. 16, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEGFP.90 beta

    Plasmid

    #108221

    Purpose

    Mammalian expression vector for EGFP fusion to human Hsp90 beta

    Depositor

    Didier Picard

    Article

    Picard et al Exp Cell Res. 2006 Nov 15. 312(19):3949-

    Insert

    EGFP-Hsp90b (HSP90AB1 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pZT-AAVS1-R1

    Plasmid

    #52638

    Purpose

    TALEN-mediated gene editing at human PPP1R12C/AAVS1 locus

    Depositor

    Mahendra Rao, Jizhong Zou

    Article

    Luo et al Stem Cells Transl Med. 2014 May 15. pii:

    Insert

    Right AAVS1 TALEN
    

    Use

    TALEN

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 12, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pZT-AAVS1-L1

    Plasmid

    #52637

    Purpose

    TALEN-mediated gene editing at human PPP1R12C/AAVS1 locus

    Depositor

    Mahendra Rao, Jizhong Zou

    Article

    Luo et al Stem Cells Transl Med. 2014 May 15. pii:

    Insert

    Left AAVS1 TALEN
    

    Use

    TALEN

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 28, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCMVTNT-T7-KPNA3

    Plasmid

    #26679

    Depositor

    Bryce Paschal

    Article

    Kelley et al BMC Cell Biol. 2010 Aug 11;11:63. doi: 1

    Insert

    Karyopherin Alpha 3 (KPNA3 Human)
    

    Tags

    T7 (MASMTGGQQMG)

    Expression

    Mammalian

    Available Since

    Jan. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pmTurquoise2-LAMP1

    Plasmid

    #110948

    Purpose

    Expresses LAMP1 fused to mTurquoise2

    Depositor

    Pablo Rivera-Fuentes

    Article

    Halabi et al Nat Commun. 2019 Mar 15;10(1):1232. doi:

    Insert

    Lysosomal-associated membrane protein 1 (LAMP1 Human)
    

    Tags

    mTurquoise2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLenti-DsRed_IRES_EGFP

    Plasmid

    #92194

    Purpose

    Lentiviral overexpression vector to make stable bicistronic cell line for control (EGFP) screen

    Depositor

    Huda Zoghbi

    Article

    Rousseaux et al Elife. 2016 Oct 25;5. pii: e19809. doi:

    Insert

    DsRed-IRES-EGFP
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    June 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCherry.90 beta

    Plasmid

    #108223

    Purpose

    Mammalian expression vector for mCherry fusion to human Hsp90 beta

    Depositor

    Didier Picard

    Article

    Picard et al Exp Cell Res. 2006 Nov 15. 312(19):3949-

    Insert

    mCherry-Hsp90b (HSP90AB1 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCMVTNT-T7-KPNA1

    Plasmid

    #26677

    Depositor

    Bryce Paschal

    Article

    Kelley et al BMC Cell Biol. 2010 Aug 11;11:63. doi: 1

    Insert

    Karyopherin Alpha 1 (KPNA1 Human)
    

    Tags

    T7

    Expression

    Mammalian

    Mutation

    KPNA1 sequence contains a reported SNP at S73N

    Available Since

    Nov. 10, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pCI-neo.ARRDC1-mCherry

    Plasmid

    #38319

    Depositor

    Quan Lu

    Article

    Nabhan et al Proc Natl Acad Sci U S A. 2012 Mar 13;10

    Insert

    ARRDC1 (ARRDC1 Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 15, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pcDNA3/Flag-METTL14

    Plasmid

    #53740

    Purpose

    For expression of METTL14 (methyltransferase-like 14) in human cells

    Depositor

    Chuan He

    Article

    Liu et al Nat Chem Biol. 2014 Feb;10(2):93-5. doi:

    Insert

    Methyltransferase-like 14 (METTL14 Human)
    

    Tags

    FLAG tag

    Expression

    Mammalian

    Promoter

    T7

    Available Since

    Oct. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pmCherry-NLS

    Plasmid

    #39319

    Depositor

    Martin Offterdinger

    Article

    Micutkova et al Int J Cancer. 2012 Apr 1;130(7):1544-57.

    Insert

    mCherry-3xNLS
    

    Tags

    3xSV40LT NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 26, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pLPCX mito Grx1-roGFP2

    Plasmid

    #64977

    Purpose

    Mammalian expression of mitochondrial Grx1-roGFP2 (retroviral vector)

    Depositor

    Tobias Dick

    Article

    Gutscher et al Nat Methods. 2008 Jun;5(6):553-9. doi: 1

    Insert

    Glutaredoxin-1 (GLRX Human)
    

    Use

    Retroviral

    Tags

    roGFP2

    Expression

    Mammalian

    Mutation

    mitochondrial targeting sequence

    Available Since

    June 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pICE-HA-MRE11-WT

    Plasmid

    #82033

    Purpose

    Plasmid for constitutive or doxycycline-inducible expression of wild-type human MRE11. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.

    Depositor

    Patrick Calsou, Sébastien Britton

    Article

    Chanut et al Nat Commun. 2016 Sep 19;7:12889. doi: 10

    Insert

    MRE11 (MRE11 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV-tet

    Available Since

    Sept. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pLPCX cyto Grx1-roGFP2

    Plasmid

    #64975

    Purpose

    Mammalian expression of cytosolic Grx1-roGFP2 (retroviral vector)

    Depositor

    Tobias Dick

    Article

    Gutscher et al Nat Methods. 2008 Jun;5(6):553-9. doi: 1

    Insert

    Glutaredoxin-1 (GLRX Human)
    

    Use

    Retroviral

    Tags

    roGFP2

    Expression

    Mammalian

    Available Since

    June 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. HBM-Luc

    Plasmid

    #35155

    Depositor

    Linda Penn

    Article

    Facchini et al Mol Cell Biol. 1997 Jan;17(1):100-14.

    Insert

    Myc promoter (MYC Human)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    Contains 2.5 kb of the c-myc minimal promoter

    Promoter

    c-myc

    Available Since

    Oct. 23, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pLenti-PGK-Hygro-amp-sgRNA-resistant PBRM1

    Plasmid

    #107407

    Purpose

    Lentiviral expression of gRNA resistant PBRM1

    Depositor

    William Kaelin

    Article

    Gao et al Proc Natl Acad Sci U S A. 2017 Jan 31;11

    Insert

    sg resistant PBRM1 (PBRM1 Human)
    

    Use

    Lentiviral

    Mutation

    sgRNA resistant

    Available Since

    May 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. AAVS1 hPGK-PuroR-pA donor

    Plasmid

    #22072

    Depositor

    Rudolf Jaenisch

    Article

    Hockemeyer et al Nat Biotechnol. 2009 Sep;27(9):851-7. do

    Insert

    PGK-Puro (PGK1 Human)
    

    Use

    Zfn donor

    Available Since

    Oct. 5, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. shPBRM1#32-Tet-pLKO-puro

    Plasmid

    #107399

    Purpose

    Lentiviral expression of shRNA targeting PBRM

    Depositor

    William Kaelin

    Article

    Gao et al Proc Natl Acad Sci U S A. 2017 Jan 31;11

    Insert

    shRNA targeting PBRM1 (PBRM1 Human)
    

    Use

    Lentiviral

    Available Since

    June 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. shPBRM1#131-Tet-pLKO-puro

    Plasmid

    #107400

    Purpose

    Lentiviral expression of shRNA targeting PBRM

    Depositor

    William Kaelin

    Article

    Gao et al Proc Natl Acad Sci U S A. 2017 Jan 31;11

    Insert

    shRNA targeting PBRM1 (PBRM1 Human)
    

    Use

    Lentiviral

    Available Since

    May 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. HMBP-3C-STAR.66-285

    Plasmid

    #100094

    Purpose

    expresses MBP-tagged STARD1 protein domain (residues 66-285)

    Depositor

    Nikolai Sluchanko

    Article

    Sluchanko et al Protein Expr Purif. 2016 Mar;119:27-35.

    Insert

    STAR protein (STAR Human)
    

    Tags

    His-tagged MBP

    Expression

    Bacterial

    Promoter

    Ptac

    Available Since

    Sept. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. GFP-p38alpha

    Plasmid

    #86832

    Purpose

    mammalian expression of GFP-p38alpha

    Depositor

    Rony Seger

    Article

    Zehorai et al Mol Cell Biol. 2014 Jan;34(2):259-70. do

    Insert

    p38alpha (MAPK14 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pCW57-GFP-P2A-MCS (Neo)

    Plasmid

    #89181

    Purpose

    All-in-one doxycycline inducible lentiviral vector for expression of one gene in combination with turbo GFP using the P2A self-cleaving peptide.

    Depositor

    Adam Karpf

    Article

    Barger et al Elife. 2021 Apr 23;10. pii: 55070. doi:

    Type

    Empty backbone

    Use

    Lentiviral; Doxycycline inducible; p2a self cleav…

    Expression

    Mammalian

    Promoter

    TRE

    Available Since

    April 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pBJ FLAG-hPar1

    Plasmid

    #53226

    Purpose

    mammalian expression of human Par1 with N-terminal FLAG tag

    Depositor

    Shaun Coughlin

    Article

    Ishii et al J Biol Chem. 1993 May 5. 268(13):9780-6.

    Insert

    Par1 (F2R Human)
    

    Tags

    FLAG and bovine prolactin signal sequence (MDSKGS…

    Expression

    Mammalian

    Mutation

    Deletion of aa1-39 (native signal peptide)

    Promoter

    SV40

    Available Since

    Aug. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pY001 (pFnCpf1_full)

    Plasmid

    #69973

    Purpose

    Expresses FnCpf1 locus including Cas genes and spacers 1-4 of CRISPR array.

    Depositor

    Feng Zhang

    Article

    Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

    Insert

    FnCpf1 locus
    

    Use

    CRISPR

    Expression

    Bacterial

    Available Since

    Oct. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pcDNA-Flag-H3.3-polyA

    Plasmid

    #59780

    Purpose

    Express Flag-tagged H3.3 (H3F3B) in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

    Depositor

    Yi Zhang

    Article

    Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

    Insert

    H3F3B (H3-3B Human)
    

    Expression

    Mammalian

    Promoter

    CMV and T7

    Available Since

    Sept. 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pAdSh.PGK.Cas9

    Plasmid

    #58253

    Purpose

    Expresses human codon-optimized S. pyogenes Cas9 from the human PGK-1 gene promoter

    Depositor

    Manuel Goncalves

    Article

    Maggio et al Sci Rep. 2014 May 29;4:5105. doi: 10.103

    Insert

    Human codon-optimized S. pyogenes Cas9
    

    Use

    Adenoviral and CRISPR

    Expression

    Mammalian

    Available Since

    Sept. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. Human_TFAM_NoMTS_pET28

    Plasmid

    #34705

    Depositor

    David Chan

    Article

    Ngo et al Nat Struct Mol Biol. 2011 Oct 30;18(11):

    Insert

    TFAM (TFAM Human)
    

    Tags

    6XHis

    Expression

    Bacterial

    Mutation

    Encodes residues 43–246, corresponding to full-le…

    Promoter

    T7

    Available Since

    Feb. 1, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pcDNA1 Flag ATXN1[85Q]

    Plasmid

    #33237

    Depositor

    Huda Zoghbi

    Article

    Skinner et al Nature. 1997 Oct 30;389(6654):971-4.

    Insert

    Ataxin-1 (ATXN1 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    insert contains a stretch of 85 Glutamines and no…

    Promoter

    CMV

    Available Since

    July 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. VP16-ER alpha

    Plasmid

    #11351

    Depositor

    Donald McDonnell

    Article

    Chang et al Mol Cell Biol. 1999 Dec . 19(12):8226-39

    Insert

    ER alpha (ESR1 Human)
    

    Expression

    Mammalian

    Available Since

    May 25, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pcDNA3-Delta(Pro-MP)ADAM17-HA

    Plasmid

    #65221

    Purpose

    Expression of HA tagged ADAM17 lacking prodomain and metalloprotease domain in mammalian cells

    Depositor

    Axel Ullrich

    Article

    Gschwind et al EMBO J. 2003 May 15;22(10):2411-21.

    Insert

    Delta(Pro-MP)ADAM17 (ADAM17 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    deleted AA 18-473

    Promoter

    CMV

    Available Since

    Nov. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. sgPBRM1-1- LentiCRISPRv2

    Plasmid

    #107403

    Purpose

    Lentiviral expression of Cas9 and gRNA targeting PBRM1

    Depositor

    William Kaelin

    Article

    Gao et al Proc Natl Acad Sci U S A. 2017 Jan 31;11

    Insert

    gRNA targeting PBRM1 (PBRM1 Human)
    

    Use

    Lentiviral

    Available Since

    June 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. sgPBRM1-2- LentiCRISPRv2

    Plasmid

    #107404

    Purpose

    Lentiviral expression of Cas9 and gRNA targeting PBRM1

    Depositor

    William Kaelin

    Article

    Gao et al Proc Natl Acad Sci U S A. 2017 Jan 31;11

    Insert

    gRNA targeting PBRM1 (PBRM1 Human)
    

    Use

    Lentiviral

    Available Since

    June 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pCW57-RFP-P2A-MCS (Neo)

    Plasmid

    #89182

    Purpose

    All-in-one doxycycline inducible lentiviral vector for expression of one gene in combination with turbo RFP using the P2A self-cleaving peptide.

    Depositor

    Adam Karpf

    Article

    Barger et al Elife. 2021 Apr 23;10. pii: 55070. doi:

    Type

    Empty backbone

    Use

    Lentiviral; Doxycycline inducible; p2a self cleav…

    Expression

    Mammalian

    Promoter

    TRE

    Available Since

    April 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. EF12J

    Plasmid

    #42943

    Depositor

    Eline Luning Prak

    Article

    Farkash et al Nucleic Acids Res. 2006 Feb 28;34(4):119

    Insert

    EN+ Human L1 element (L1RE1 Human)
    

    Tags

    EGFP (opposite strand)

    Expression

    Mammalian

    Mutation

    AgeI-BclI fragment swapped with nt 1927-3708 from…

    Promoter

    CMV MIE, L1 5'UTR

    Available Since

    April 25, 2013

    Availability

    Academic Institutions and Nonprofits only

49. pcDNA1 Flag ATXN1[30Q]

    Plasmid

    #33236

    Depositor

    Huda Zoghbi

    Article

    Skinner et al Nature. 1997 Oct 30;389(6654):971-4.

    Insert

    Ataxin-1 (ATXN1 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    insert contains a stretch of 29 amino acids (27 Q…

    Promoter

    CMV

    Available Since

    July 18, 2014

    Availability

    Academic Institutions and Nonprofits only

50. GFP-JNK1

    Plasmid

    #86830

    Purpose

    mammalian expression of GFP-JNK1

    Depositor

    Rony Seger

    Article

    Zehorai et al Mol Cell Biol. 2014 Jan;34(2):259-70. do

    Insert

    JNK1 (MAPK8 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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