167,910 results

1. pDEST exorh:mCherry

   Plasmid

   #195989

   Purpose

   Transgenesis backbone

   Depositor

   Nicolas Rohner, Christian Mosimann

   Article

   Kemmler et al Development. 2023 Mar 28:dev.201531. doi

   Type

   Empty backbone

   Use

   Other

   Available Since

   Feb. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. RMCE halo-flag DNA-PKcs

   Plasmid

   #233918

   Purpose

   Low copy number provides expression of human DNA-PKcs with an N-terminal halo-flag tag.

   Depositor

   Katheryn Meek

   Article

   Buehl et al Mol Cell. 2023 Mar 2;83(5):698-714.e4. d

   Insert

   human DNA-PKcs (PRKDC Human)
   

   Tags

   flag-halo

   Expression

   Mammalian

   Available Since

   May 12, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3-EGFP-Cdc42-Q61L

   Plasmid

   #12986

   Depositor

   Gary Bokoch

   Article

   Subauste et al J Biol Chem. 2000 Mar 31. 275(13):9725-3

   Insert

   Cdc42 constitutively active (CDC42 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Q61L Constitutively active

   Available Since

   Oct. 27, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1-3XHA-p53

   Plasmid

   #196267

   Purpose

   Mammalian expression of 3xHA tagged wild-type p53

   Depositor

   Laura Attardi

   Article

   Raj et al Mol Cell. 2022 Jul 7;82(13):2370-2384.e1

   Insert

   p53 (Trp53 Mouse)
   

   Tags

   3xHA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHR_Gal4UAS_pGK_mCherry

   Plasmid

   #79124

   Purpose

   Lentiviral vector for inducible expression of genes with Gal4DBDVP64 synNotch Receptors (no IRES)

   Depositor

   Wendell Lim

   Article

   Morsut et al Cell. 2016 Feb 11;164(4):780-91. doi: 10

   Type

   Empty backbone

   Use

   Lentiviral

   Available Since

   July 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV2-DEST(r)

   Plasmid

   #190238

   Purpose

   Gateway destination vector, empty AAV vector backbone

   Depositor

   Hiroyuki Hioki

   Article

   Sohn et al PLoS One. 2017 Jan 6;12(1):e0169611. doi

   Type

   Empty backbone

   Use

   AAV

   Expression

   Mammalian

   Available Since

   Nov. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pKAR2-Br512_Mut235

   Plasmid

   #179278

   Purpose

   Expresses Br512_Mut235 polymerase in E.coli

   Depositor

   Andrew Ellington

   Article

   Paik et al Biochemistry. 2021 Nov 11. doi: 10.1021/

   Insert

   Engineered Bst DNA Polymerase I Large Fragment
   

   Tags

   8x His tag; Villin Headpiece 47 amino acid (HP47)…

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. TRE3G-PE2-P2A-BFP

   Plasmid

   #231580

   Purpose

   Expresses the PE2 prime-editing machinery fused to BFP (PE2-P2A-BFP), under the control of a TRE3G promoter. This promoter is responsive to doxycycline bound to the rtTA protein

   Depositor

   Jesse Engreitz

   Article

   Martyn et al bioRxiv [Preprint]. 2023 Dec 21:2023.12.

   Insert

   PE2-P2A-BFP
   

   Use

   Lentiviral

   Tags

   P2A-BFP

   Expression

   Mammalian

   Promoter

   TRE3G

   Available Since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pREP-8xARE-GFP-SV40-BFP

   Plasmid

   #134910

   Purpose

   NRF2 reporter plasmid with GFP under control of 8 ARE elements and constitutively transcribed BFP

   Depositor

   Markus Landthaler

   Article

   Wyler et al Nat Commun. 2019 Oct 25;10(1):4878. doi:

   Inserts

   8xARE- minimal promoter - GFP
   TagBFP
   

   Expression

   Mammalian

   Promoter

   8xARE - minimal promoter and SV40

   Available Since

   Jan. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLV-CMV-LoxP-DsRed-LoxP-eGFP

    Plasmid

    #65726

    Purpose

    Functions as a genetic Cre reporter that switches from DsRed expression to eGFP expression upon presence of Cre recombinase.

    Depositor

    Jacco van Rheenen

    Article

    Zomer et al Cell. 2015 May 21;161(5):1046-57. doi: 1

    Insert

    LoxP-DsRed-LoxP-eGFP
    

    Use

    Cre/Lox and Lentiviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHR_PGK_antiHer24D5-7_synNotch_Gal4VP64

    Plasmid

    #85424

    Purpose

    Lentiviral vector for expression of anti-Her2 scFv 24D5-7 Gal4VP64 synthetic Notch

    Depositor

    Wendell Lim

    Article

    Roybal et al Cell. 2016 Oct 6;167(2):419-432.e16. doi

    Insert

    anti-Her2_4D5-7_synNotch
    

    Use

    Lentiviral

    Promoter

    PGK

    Available Since

    Sept. 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBABE-puro-hTERT-HA

    Plasmid

    #1772

    Depositor

    Bob Weinberg

    Article

    Counter et al Proc Natl Acad Sci U S A 1998 Dec 8;95(2

    Insert

    hTERT (TERT Human)
    

    Use

    Retroviral

    Tags

    2XHA

    Expression

    Mammalian

    Available Since

    June 3, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSems LifeAct-StayGold

    Plasmid

    #222947

    Purpose

    Expression of LifeAct tagged with StayGold in mammalian cells

    Depositor

    Jacob Piehler

    Article

    Winkelmann et al Nat Commun. 2024 Jul 10;15(1):5813. doi:

    Insert

    LifeAct
    

    Tags

    StayGold

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. plentiCMV-sp-HPV-TM(ICAM)

    Plasmid

    #196510

    Purpose

    Express HPV antigen epitope with ICAM transmembrane domain

    Depositor

    Howard Chang

    Article

    Yu et al Cell. 2022 Dec 22;185(26):4904-4920.e22.

    Insert

    a HPV epitope with CD8a stalk linker and ICAM TM domain
    

    Use

    Lentiviral

    Tags

    3xflag tag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCAS9-mCherry-Frame +1

    Plasmid

    #66940

    Purpose

    CRISPaint frame selector +1

    Depositor

    Veit Hornung

    Article

    Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

    Insert

    gRNA frame +1
    

    Available Since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pP2A-mCherry-N1

    Plasmid

    #84329

    Purpose

    Encodes P2A-mCherry preceded by a multiple cloning sequence. Enables bicistronic expression of protein of interest and mCherry

    Depositor

    Dorus Gadella

    Article

    van Unen et al Mol Pharmacol. 2016 Sep;90(3):162-76. do

    Insert

    P2A-mCherry
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. MSCV-Luciferase-IRES-NrasG12D

    Plasmid

    #60834

    Purpose

    Mouse NrasG12D was amplified and mutated by PCR from IMAGE clone 6475312 (Invitrogen) and cloned downstream from the IRES; subsequently Luciferase was cloned upstream of the IRES

    Depositor

    Scott Lowe

    Article

    Zuber et al Genes Dev. 2009 Apr 1;23(7):877-89. doi:

    Insert

    NrasG12D (Nras Mouse)
    

    Use

    Retroviral

    Available Since

    Nov. 13, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEGFP-N2-COL1A1

    Plasmid

    #66602

    Purpose

    Expresses procollagen 1 alpha 1 as a GFP fusion

    Depositor

    David Stephens

    Article

    Stephens et al J Cell Sci. 2002 Mar 15;115(Pt 6):1149-6

    Insert

    COL1A1 (COL1A1 Human)
    

    Expression

    Mammalian

    Available Since

    July 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pUC18R6K-mini-Tn7T-Gm

    Plasmid

    #65022

    Purpose

    GmR mini-Tn7 vector, R6K replicon

    Depositor

    Herbert Schweizer

    Article

    Choi et al Nat Protoc. 2006;1(1):153-61.

    Type

    Empty backbone

    Available Since

    Aug. 17, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. Flag-HA-GFP

    Plasmid

    #22612

    Depositor

    Wade Harper

    Article

    Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

    Insert

    GFP
    

    Use

    Retroviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    None

    Available Since

    Dec. 4, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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21. EGFP-GRAM-W

    Plasmid

    #211701

    Purpose

    Expression of EGFP-tagged GRAM domain of GRAMD1b carrying a G187W mutation (EGFP-GRAM1b G187W) in mammalian cells

    Depositor

    Yasunori Saheki

    Article

    Koh et al Nat Commun. 2023 Oct 25;14(1):6773. doi:

    Insert

    GRAM domain of GRAMD1b/Aster-B (GRAMD1B Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Changed Glycine 187 to Tryptophan

    Promoter

    CMV

    Available Since

    Jan. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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22. pAAV-Ef1a-mCherry (AAV Retrograde)

    Viral Prep

    #114470-AAVrg

    Purpose

    Ready-to-use AAV Retrograde particles produced from pAAV-Ef1a-mCherry (#114470). In addition to the viral particles, you will also receive purified pAAV-Ef1a-mCherry plasmid DNA. EF1a-driven mCherry expression. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Karl Deisseroth

    Promoter

    EF1a

    Tags

    mCherry

    Available Since

    Sept. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

23. pCMV mRuby3-Galectin-3

    Plasmid

    #215375

    Purpose

    Expression of the endolysosomal damage marker Galectin-3 with an N-terminal mRuby3 tag.

    Depositor

    Franz-Ulrich Hartl

    Article

    Sitron et al Mol Cell. 2025 Sep 18;85(18):3505-3523.e

    Insert

    LGALS3 (LGALS3 Human)
    

    Expression

    Mammalian

    Available Since

    Aug. 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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24. L4440_BioBrick-sgRNA

    Plasmid

    #177783

    Purpose

    Overexpression of sgRNAs in E. coli HT115, sgRNA overexpression scaffold; for ingestion by C. elegans

    Depositor

    Michael Ristow

    Article

    Fischer et al J Biol Chem. 2022 May 27:102085. doi: 10

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    June 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. 763 pME mApple

    Plasmid

    #195960

    Purpose

    Gateway pME fluorophore

    Depositor

    Kristen Kwan, Christian Mosimann

    Article

    Kemmler et al Development. 2023 Mar 28:dev.201531. doi

    Insert

    mApple
    

    Use

    Other

    Available Since

    Feb. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pAAV-Ef1a-DIO EYFP (AAV Retrograde)

    Viral Prep

    #27056-AAVrg

    Purpose

    Ready-to-use AAV Retrograde particles produced from pAAV-Ef1a-DIO EYFP (#27056). In addition to the viral particles, you will also receive purified pAAV-Ef1a-DIO EYFP plasmid DNA. EF1a-driven, Cre-dependent EYFP expression. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Karl Deisseroth

    Tags

    EYFP (Cre-dependent)

    Available Since

    Jan. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

27. pAAV-TREtight-mTagBFP2-B19G

    Plasmid

    #100799

    Purpose

    helper virus for monosynaptic tracing; to be coinjected with pAAV-syn-FLEX-splitTVA-EGFP-tTA

    Depositor

    Ian Wickersham

    Article

    Liu et al Nature. 2017 Aug 31;548(7669):582-587. d

    Has Service

    AAV1

    Insert

    mTagBFP2 and rabies virus SAD B19 strain glycoprotein genes (B19G)
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    TREtight

    Available Since

    Oct. 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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28. 24xMoonTag-kif18b-24xPP7 (MoonTag translation reporter)

    Plasmid

    #128604

    Purpose

    Expresses 24xgp41 peptide array (MoonTag peptide array), followed by kif18b gene and 24 repeats of PP7 hairpins in 3’ UTR.

    Depositor

    Marvin Tanenbaum

    Article

    Boersma et al Cell. 2019 Jun 5. pii: S0092-8674(19)304

    Inserts

    24xMoonTag
    kinesin family member 18b (KIF18B Human)
    

    Expression

    Mammalian

    Promoter

    Pcmv

    Available Since

    Aug. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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29. pT3-EF1a-YapS127A

    Plasmid

    #46049

    Depositor

    Xin Chen

    Article

    Tschaharganeh et al Gastroenterology. 2013 Jun;144(7):1530-1

    Insert

    Yes-kinase associated protein (YAP1 Human)
    

    Tags

    Flag tag

    Expression

    Mammalian

    Mutation

    S127A

    Promoter

    EF1a

    Available Since

    July 2, 2013

    Availability

    Academic Institutions and Nonprofits only

30. pLV[Exp]-EF1A>Tet3G:IRES:Neo

    Plasmid

    #198756

    Purpose

    Vector encodes a Tet-On Advanced transactivator under control of a constitutive EF1α promoter and confers resistance to the antibiotic G418

    Depositor

    Nael Nadif Kasri

    Article

    Mossink et al Mol Psychiatry. 2022 Jan;27(1):1-18. doi

    Insert

    Tet3G, IRES, Neo
    

    Use

    Lentiviral

    Promoter

    EF1A

    Available Since

    July 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pCGN-HCF-1 fl

    Plasmid

    #53309

    Purpose

    plasmid contains human HCFC1 full length gene with HA and c-myc tags

    Depositor

    Winship Herr

    Article

    Wilson et al Cell. 1993 Jul 16;74(1):115-25.

    Insert

    HCFC1 (HCFC1 Human)
    

    Tags

    HA and c-myc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 28, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pDONR223-ERBB2

    Plasmid

    #23888

    Depositor

    David Root, William Hahn

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    ERBB2 (ERBB2 Human)
    

    Use

    Gateway donor vector

    Available Since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

33. HR180-LGR5-GFP

    Plasmid

    #129092

    Purpose

    KI EGFP with a puromycin/mRuby selection cassette into human LGR5 locus

    Depositor

    Toshiro Sato

    Article

    Shimokawa et al Nature. 2017 May 11;545(7653):187-192. d

    Insert

    EGFP and LGR5 homology arms (LGR5 Human)
    

    Use

    Homologous recombination donor plasmid

    Available Since

    Sept. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. RMCE K>R DNA-PKcs

    Plasmid

    #233917

    Purpose

    Low copy number provides expression of human DNA-PKcs with a single K>R substitution that ablates kinase activity.

    Depositor

    Katheryn Meek

    Article

    Buehl et al Mol Cell. 2023 Mar 2;83(5):698-714.e4. d

    Insert

    human DNA-PKcs kinase inactive mutant K3753>R (PRKDC Human)
    

    Expression

    Mammalian

    Mutation

    K3753R

    Available Since

    May 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pAcGP67A-mMD2-proA

    Plasmid

    #187878

    Purpose

    baculovirus protein expression of mouse MD-2 (residues 19–160) fused to a protein A tag

    Depositor

    Bruce Beutler

    Article

    Wang et al Proc Natl Acad Sci U S A. 2016 Feb 16;11

    Insert

    MD2 (Ly96 Mouse)
    

    Expression

    Insect

    Mutation

    includes residues 19–160 of mouse MD-2

    Available Since

    July 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pDisplay-pHuji

    Plasmid

    #61556

    Purpose

    Red fluorescent protein based pH indicator in pDisplay vector

    Depositor

    Robert Campbell

    Article

    Shen et al J Cell Biol. 2014 Nov 10;207(3):419-32.

    Insert

    pHuji
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 19, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pCMV-hERalpha

    Plasmid

    #101141

    Purpose

    Mammalian expression of full-length human estrogen receptor-alpha

    Depositor

    Elizabeth Wilson

    Article

    Mao et al J Biol Chem. 2008 May 9;283(19):12819-30

    Insert

    estrogen receptor-alpha (ESR1 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. gag/pol

    Plasmid

    #14887

    Depositor

    Tannishtha Reya

    Article

    Reya et al Nature. 2003 May 22. 423(6938):409-14.

    Insert

    gag / pol
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    Aug. 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pMSCV-dCasRx-ALKBH5-PURO

    Plasmid

    #175582

    Purpose

    Expresses dCasRx-ALKBH5 in mammalian cell

    Depositor

    Qi Xie

    Article

    Xia et al Nucleic Acids Res. 2021 Jul 21;49(13):73

    Insert

    dCasRx-ALKBH5
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG-HA

    Expression

    Mammalian

    Mutation

    RfxCas13d R239A/H244A/R858A/H863A

    Promoter

    pMSCV

    Available Since

    Nov. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. Yarrowia lipolytica Golden Gate tool kit

    Plasmid Kit

    #1000000167

    Purpose

    Yarrowia lipolytica Golden Gate tool kit for integrative vector assembly of up to three transcription units in the oleaginous yeast Y. lipolytica.

    Depositor

    Tristan Rossignol

    Available Since

    Jan. 20, 2021

    Availability

    Academic Institutions and Nonprofits only