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MSCV-miRE_shBRD9_561-SV40-GFP
Plasmid#75139PurposeRetroviral expression plasmid encoding shBRD9_561 (targeting human BRD)DepositorInsertshBRD9_561
UseRetroviralTagsExpressionMutationPromoterAvailable sinceMay 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
TFORF2556
Plasmid#144042PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertADNP2 (ADNP2 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJuly 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
Tet-inducible- FLAG-Core R (pMO30)
Plasmid#24479DepositorInsertCore R
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceApril 23, 2010AvailabilityAcademic Institutions and Nonprofits only -
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pDONR223_SPOP_p.E47A
Plasmid#81645PurposeGateway Donor vector containing SPOP, part of the Target Accelerator Plasmid Collection.DepositorInsertSPOP (SPOP Human)
UseGateway entry vectorTagsExpressionMutationE47APromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
ABCG1_pCSdest
Plasmid#53952DepositorInsertABCG1 (ABCG1 Human)
UseTagsExpressionMammalianMutationPromoterSP6 and CMVAvailable sinceMarch 27, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCfB2798
Plasmid#63659PurposepCfB2798 is a vector for multiple integrations at sites sharing homology with Ty4Cons. The selective marker is Kl.LEU2. Additionally it contains a PTEF1-GFP as reporter.DepositorInsertGFP
UseCre/LoxTagsExpressionYeastMutationPromoterTEF1 promoterAvailable sinceAug. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_SPOP_p.K134N
Plasmid#81647PurposeGateway Donor vector containing SPOP, part of the Target Accelerator Plasmid Collection.DepositorInsertSPOP (SPOP Human)
UseGateway entry vectorTagsExpressionMutationK134NPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEYFP-hDF C2B
Plasmid#72583Purposehuman dysferlin C2B domain tagged with YFPDepositorInsertdysferlin C2B domain (DYSF Human)
UseTagsEYFPExpressionMammalianMutationC2B domainPromoterCMVAvailable sinceApril 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-mAV
Plasmid#129472PurposeMammalian expression of full-length mouse archvillin (Supervillin, isoform 2)DepositorInsertSupervillin, isoform 2 (Svil Mouse)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceAug. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-h-mmPtch1-B-HA-TagBFP
Plasmid#120910PurposeExpresses BFP tagged Ptch1-BDepositorInsertPtch1 (Ptch1 Mouse)
UseTagsHA and TagBFPExpressionMammalianMutationdeleted amino acids 620-643, truncated at 1291PromoterCMVAvailable sinceJan. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRK5_mEGFP-NUP98-DDX10
Plasmid#237640PurposeFor overexpression of mEGFP-NUP98-DDX10DepositorUseTagsmEGFPExpressionMammalianMutationPromoterAvailable sinceMay 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRK5_mEGFP-NUP98-HOXA9
Plasmid#237639PurposeFor overexpression of mEGFP-NUP98-HOXA9DepositorUseTagsmEGFPExpressionMammalianMutationPromoterAvailable sinceMay 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28b_H6-Smt3-AesC-GFP
Plasmid#234486PurposeSuperfolder GFP C-terminally fused to His-Smt3-tagged AesC split inteinDepositorInsertC-terminal Fragment of the Aes123 PolB1 Split Intein
UseTagsHexahistidine Tag, Superfolder Green Fluorescent …ExpressionBacterialMutationPromoterAvailable sinceMay 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMAL-c2X_MBP-CLmN-H6
Plasmid#234487PurposeMaltose binding protein N-terminally fused to His-tagged CLmN split inteinDepositorInsertN-terminal Fragment of the CLm Split Intein
UseTagsHexahistidine Tag and Maltose Binding Protein (MB…ExpressionBacterialMutationT69K, F75H, M118N within the N-terminal fragment …PromoterAvailable sinceMay 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLV_EF1aUBA3_IRES_Puro
Plasmid#235660Purposehuman UBA3 cloned into pLV-EF1a-IRES-Puro vector (Addgene: 85132)DepositorInsertUBA3 (UBA3 Human)
UseLentiviralTagsExpressionMutationPromoterEF1aAvailable sinceApril 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLV_EF1a-NAE1_IRES_Puro
Plasmid#235659Purposehuman NAE1 cloned into into lentiviral backbone (pLV-EF1a-IRES-Puro)DepositorInsertNAE1 (NAE1 Human)
UseLentiviralTagsExpressionMutationPromoterEF1aAvailable sinceApril 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SET
Plasmid#234708Purposeexpresses His-tagged human SET in E coliDepositorInsertSET (SET Human)
UseTags(His)6ExpressionBacterialMutationPromoterlacIAvailable sinceApril 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
dCas9-NUP98a_IDR-EGFP
Plasmid#232766PurposeExpresses dCas9-NUP98a_IDR-EGFPDepositorInsertdCas9-NUP98a_IDR-EGFP (NUP98 Human)
UseLentiviralTagsExpressionMutationPromoterEF1aAvailable sinceApril 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSM.wrmScarlet1-10::SL2::GFP1-10
Plasmid#234318PurposeFor the expression of wrmScarlet1-10 and GFP1-10 in C. elegansDepositorInsertwrmScarlet1-10::SL2::GFP1-10
UseTagsExpressionWormMutationPromoterAvailable sinceMarch 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEN017-SpCas9-hiNLS-t-M4
Plasmid#234455PurposeExpresses hiNLS variant t-M4 inside 0C triNLS-SpCas9-NGGDepositorInsertSpCas9 hiNLS t-M4
UseCRISPRTagsHis6, Nucleoplasmin, SV40, and c-MycExpressionBacterialMutationCys at positions 80 and 574 have been mutated to …PromoterAvailable sinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEN019-SpCas9-hiNLS-t-M1M4
Plasmid#234456PurposeExpresses hiNLS variant t-M1M4 inside 0C triNLS-SpCas9-NGGDepositorInsertSpCas9 hiNLS t-M1M4
UseCRISPRTagsHis6, Nucleoplasmin, SV40, and c-MycExpressionBacterialMutationCys at positions 80 and 574 have been mutated to …PromoterAvailable sinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
Orai1-jGCaMP8f
Plasmid#231630PurposeGreen fluorescent reporter of Orai1-associated calcium influxDepositorInsertOrai1-jGCaMP8f (ORAI1 Synthetic, Human)
UseTagsjGCaMP8fExpressionMammalianMutationPromoterCMV Immediate EarlyAvailable sinceFeb. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
10279-M28-663
Plasmid#225662PurposeLentivirus protein expression. Matched control construct for 8522-M01-663 (Addgene # 225660) gives basal expression from mCMVp for gating/thresholdingDepositorInsertdsCopGFP
UseLentiviralTagsExpressionMutationPromotermCMVpAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
18065-M01-412
Plasmid#225664PurposeLentiviral expression of FLAG-tagged fluorescent proteins for immunohistochemical detection in FFPE tissueDepositorInsertsUseLentiviralTags3xFLAGExpressionMutationC-terminally truncated (aa 1-333 only)PromoterSORE6-mCMVp and WPRE-SV40pAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
TFORF0059
Plasmid#141500PurposeLentiviral vector for overexpressing the CTBP1 transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertCTBP1 (CTBP1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceDec. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
Human RNF149-3X FLAG
Plasmid#225332PurposeLentiviral expression of human RNF149 with 3x FLAG tag in mammalian cellsDepositorInsertRNF149 (RNF149 Human)
UseLentiviralTags3xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceSept. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pENN456_TALEcharm_Kv2_MmT8x
Plasmid#220835PurposeTALEcharm expression in mammalian cellsDepositorInsertTALEcharm Kv2
UseLentiviral and TALENTagsP2A-TagBFPExpressionMammalianMutationN/APromoterEF1aAvailable sinceJuly 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGGG-Sr43
Plasmid#186974PurposeSr43 is a wheat stem rust resistance gene transferred from tall wheat grass (Thinopyrum ponticum) into bread wheat (Triticum aestivum).DepositorInsertSr43
UseTagsNoneExpressionBacterial and PlantMutationNonePromoterNaticeAvailable sinceApril 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/FRT/TO_COX8-Halo-PanK
Plasmid#197949PurposeExpression of FRET_control in mammalian cellsDepositorInsertHalo-ecPanK
UseTagsExpressionMammalianMutationPanK=PanK-D127A,L277W,F252YPromoterAvailable sinceMarch 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-CLEC7A-COMP5AP-AviTag-9xHis
Plasmid#157150PurposeMammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.DepositorInsertCLEC7A (CLEC7A Human)
UseTagsCOMP5AP-AviTag-9xHisExpressionMammalianMutationPromoterCMV/SP6Available sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-CLEC7A-Fc(DAPA)-AviTag-6xHis
Plasmid#156586PurposeMammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.DepositorInsertCLEC7A (CLEC7A Human)
UseTagsFc(DAPA)-AviTag-6xHisExpressionMammalianMutationFc region of human IgG contains D265A and P329A m…PromoterCMV/SP6Available sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pVITRO1-TagRFP-NLS
Plasmid#184446PurposeNucleus-targeted TagRFP expression in mammalian cellsDepositorInsertTagRFP-3xNLS
UseTagsExpressionMammalianMutationWTPromoterRat EF1αAvailable sinceJuly 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pVITRO1-mCardinal-NES
Plasmid#184453PurposeCytosolic mCardinal expression in mammalian cellsDepositorInsertmCardinal-NES
UseTagsExpressionMammalianMutationWTPromoterRat EF1αAvailable sinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pLenti-VQR-P2A-GFP-PGK-Puro
Plasmid#110861PurposeLentiviral vector for constitutive expression of Cas9-VQR-P2A-GFP (not codon optimized)DepositorInsertCas9-VQR
UseLentiviralTags3X FLAGExpressionMutationD1135V, R1335Q, T1337RPromoterEF1sAvailable sinceJune 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLentiRNAGuide_001 - hU6-RfxCas13d-DR1-BsmBI-EFS-Puro-WPRE
Plasmid#138150PurposeFor cloning of guide RNAs libraries compatible with RfxCas13d. Contains a 5' modified direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaaDepositorInserthU6_Rfxcas13d-DR1_BsmBI
UseLentiviralTagsExpressionMutationDR1 A>TPromoterAvailable sinceFeb. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSecUAG-Evol2
Plasmid#163148PurposeExpresses machinery necessary for selenocysteine incorporation at UAG codonsDepositorInsertsallo-tRNA UTu2D
thioredoxin
Selenophosphate synthase
Selenocysteine synthase
Cysteine desulfurase
UseTagsExpressionBacterialMutationCysteine 32 switched to Selenocysteine (UAG), Cys…PromoterEM7, araBAD, native As SelD promoter, and native …Available sinceApril 28, 2021AvailabilityAcademic Institutions and Nonprofits only
