We narrowed to 17,604 results for: ef1
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Plasmid#193465PurposeDn29 Donor with promoter for mcherry and puro expressionDepositorInsertDn29_attP
ExpressionMammalianPromoterEf1aAvailable SinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJRH-1365 LV EF1a-CD28 IRES-EGFP
Plasmid#201921Purpose2nd generation lentiviral transfer plasmid for engineering cells to express human CD28DepositorAvailable SinceAug. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-DIO-APEX.NES-P2A-EGFP
Plasmid#182824PurposeCre-dependent expression of cytosolic APEXDepositorInsertAPEX.NES-P2A-EGFP
UseAAVTagsEGFP reporter (P2A)PromoterEF1aAvailable SinceMay 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-DO-hChR2(H134R)-mCherry-WPRE-pA
Plasmid#37082PurposeCre-dependent expression of hChR2(H134R) for optogenetic excitationDepositorInserthChr2(H134R)-mCherry
UseAAV and Cre/Lox; Cre-offTagsmCherryPromoterEF1aAvailable SinceJune 14, 2012AvailabilityAcademic Institutions and Nonprofits only -
pJRH-1363 LV EF1a-CD19 IRES-EGFP
Plasmid#201919Purpose2nd generation lentiviral transfer plasmid for engineering cells to express human CD19DepositorAvailable SinceAug. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pU6-sgRNA EF1Alpha-puro-T2A-BFP
Plasmid#60955PurposeParental vector for the CRISPRi/a libraries. Expresses an sgRNA from the U6 promoter and a puromycin resistance cassette and BFP from the EF1Alpha promoterDepositorInsertssgGFP-NT2
puro-T2A-BFP
UseCRISPR and LentiviralExpressionMammalianAvailable SinceJan. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
plenti-ef1a-dCasRx-FTO-HA-T2A-BSD
Plasmid#177120PurposedCasRx mediated RNA tethering of FTODepositorInsertdCasRx-FTO
UseLentiviralTagsHA and SV40 NLSMutationdCasRX contains R239A/H244A/R858A/H863A mutations…PromoterEf1aAvailable SinceNov. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
CAPTURE2_pLVX-EF1a-dCas9-CBio-IRES-zsGreen1
Plasmid#138418PurposeCAPTURE2.0 vector containing dCas9-CBio, IRES and zsGreen1DepositorInsertdCas9
UseLentiviralTagsBioTAP-tagPromoterEF1aAvailable SinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDH-EF1-TfR20-SNAP-IRES-Puro
Plasmid#171018PurposeLentiviral expression of a transferrin receptor-SNAP-tag fusion in mammalian cellsDepositorInsertTransferrin receptor (Human) - SNAP-tag (TFRC Human)
UseLentiviralTagsSNAPtag and TfRExpressionMammalianPromoterEF1Available SinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1α-F-FLEX- Kir2.1-T2A-tdTomato
Plasmid#60661PurposeFlp-dependent expression of Kir2.1-T2A-tdTomatoDepositorAvailable SinceNov. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLenti-EF1a-Luciferase-IRES-Blast-WPRE
Plasmid#108542PurposeLentivirus vector expressing Luciferase with blasticidin resistanceDepositorInsertsLuciferase
Neomycin
UseLentiviralPromoterEF1apha and PGKAvailable SinceApril 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-DIO eNpHR 3.0-EYFP
Plasmid#26966PurposeAAV expression of eNpHR 3.0 fused to EYFP driven by EF1a promoter for optogenetic inhibitionDepositorHas ServiceAAV Retrograde, AAV1, AAV5, and AAV9InserteNpHR 3.0
UseAAVTagsEYFPExpressionMammalianMutationER export at 3' end of gene. Trafficking sig…PromoterEF1alphaAvailable SinceJan. 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
pHR_EF1a-->myc-LaG17-tTA synNotch (LA1)
Plasmid#162230PurposeLentiviral vector for the expression of GFP nanobody LaG17 tTA SynNotch: pHR_EF1a-->myc-LaG17-tTA synNotch (LA1) in mammalian cellsDepositorInsertLaG17 GFP nanobody SynNotch tTA
UseLentiviralExpressionMammalianAvailable SinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-DIO ChRmine-eYFP-WPRE
Plasmid#130996PurposeDouble floxed ChRmine-eYFP under the control of Ef1a promoterDepositorInsertChRmine
UseAAVTagseYFPPromoterEf1aAvailable SinceSept. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti-hSFTPCpromoter(2kb)-EGFP-EF1a-TagRFP
Plasmid#201681PurposeLentiviral vector for human SFTPC promoter (2kb)-driven expression of EGFP and EF1a-driven TagRFP expressionDepositorInsertsSFTPC promoter region (2kb upstream)
TagRFP
UseLentiviralExpressionMammalianAvailable SinceNov. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAV-EF1A-DIO-Gephyrin.FingR-eGFP-CCR5TC
Plasmid#126217PurposeLabeling of inhibitory synapses (green fluorescence) in cre-expressing neuronsDepositorInsertGephyrin.FingR-eGFP-CCR5TC
UseAAV and Cre/LoxExpressionMammalianPromoterEF1AAvailable SinceJuly 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EF1a-GFP-LaminA-IRES-Hygromycin
Plasmid#134867PurposeExpresses EGFP-tagged nuclear envelope markerDepositorAvailable SinceMarch 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pICOZ-MAG[EF1α-CD19 4-1BB-eGFP]
Plasmid#218344PurposeDonor plasmid of MAG Transposon with CD19 CAR mammalian expression cassetteDepositorInsertCD19 CAR mammalian expression cassette
UseLentiviralExpressionMammalianAvailable SinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHR-UCOE-EF1a-Zim3-dCas9-BFP
Plasmid#188775PurposedCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLSDepositorInsertZim3-dCas9-mTagBFP2
UseLentiviralTagsHA-2xNLS-mTagBFP2 and Zim3 KRAB-NLS fusionPromoterEF1aAvailable SinceOct. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti-tetON-NOTUM-EF1a-TagRFP-2A-tet3G
Plasmid#204188PurposeDoxycycline inducible lentiviral vector for human NOTUM overexpressionDepositorAvailable SinceAug. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-fDIO hChR2(H134R)-EYFP
Plasmid#55639PurposeFlp-Dependent ChR2-EYFPDepositorHas ServiceAAV RetrogradeInserthChR2(H134R)-EYFP
UseAAVTagsEYFPExpressionMammalianPromoterEf1aAvailable SinceAug. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EF1a-EGFP-RAB7A-IRES-Puromycin
Plasmid#133027PurposeExpresses EGFP-tagged late endosome markerDepositorInsertRAB7A (RAB7A Human)
UseLentiviralTagsEGFPExpressionMammalianPromoterHuman elongation factor 1 alphaAvailable SinceFeb. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS DRR mCherry Donor EF1a BFP
Plasmid#98896PurposeHomologous recombination donor for pDRR. Expresses mCherry after repait by HR.DepositorInsertNeomycin-T2A-mCherry-GFP
UseSynthetic BiologyExpressionMammalianAvailable SinceNov. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EF1a-EGFP-RAB5A-IRES-Puromycin
Plasmid#134858PurposeExpresses EGFP-tagged early endosome markerDepositorInsertRas-related protein 5A
UseLentiviralTagsEGFPExpressionMammalianPromoterEF1aAvailable SinceMarch 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPV-Dual_promoter-EF1α-2xNLS-Cascade+Cas3-P (RD)
Plasmid#204619PurposeExpression vector of Cascade and Cas3. Puromycin selectable.DepositorInsertCas7, Cas5, Cas8, Cas11, Cas6, and Cas3
UseCRISPRTagsEach protein has C- and N-terminal SV40 NLSPromoterEF1aAvailable SinceAug. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
Ef1a-pspCas13b-NES-3xFLAG-T2A-BFP
Plasmid#173029PurposeFor expression of cytoplasmic pspCas13b protein tagged with 3xHA-T2A-BFP for gene silencing in mamallian cells.DepositorInsertpspCas13b
UseCRISPR and LentiviralTags3xFLAG, BFP, HIV NES, and T2AExpressionMammalianPromoterEf1aAvailable SinceSept. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
Lenti-(BB)-EF1a-KRAB-dCas9-P2A-BlastR
Plasmid#118154PurposeCatalytically inactive Cas9 from S. pyogenes with P2A-BlastR under the EF1a core promoter, and cloning backbone for sgRNA. Contains BsmBI sites for insertion of spacer sequences.DepositorInsertKRAB-dCas9-P2A-BlastR
UseCRISPR and LentiviralTagsHAExpressionMammalianMutationSee depositor comments belowPromoterEF1a core and U6Available SinceMarch 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti-tetON-NKX2-1-EF1a-TagRFP-2A-tet3G
Plasmid#167942PurposeInducible NKX2-1 overexpressionDepositorAvailable SinceJune 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJRH-1364 LV EF1a-CD4 IRES-EGFP
Plasmid#201920Purpose2nd generation lentiviral transfer plasmid for engineering cells to express human CD4DepositorAvailable SinceAug. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pU6-sgRNA Ef1alpha Puro-T2A-GFP
Plasmid#111596PurposesgRNA Ef1alpha Puro-T2A-GFPDepositorInsertmU6-sgRNA
UseCRISPR and LentiviralExpressionMammalianAvailable SinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-Con/Foff 2.0-EYFP
Plasmid#137162PurposeIntersectional viral expression of EYFP in cells expressing Cre AND NOT FlpDepositorHas ServiceAAV8InsertCon/Foff 2.0-EYFP
UseAAV, Cre/Lox, and Synthetic Biology ; Flp/frtMutationn/aPromoterEF1aAvailable SinceJune 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EF1a-LAMP-1-mGFP-IRES-Puromycin
Plasmid#134868PurposeExpresses EGFP-tagged lysosomal markerDepositorInsertLysosomal-associated membrane protein 1 (LAMP1 Human)
UseLentiviralTagsGFPExpressionMammalianPromoterEF1aAvailable SinceMarch 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAV-EF1A-PSD95.FingR-eGFP-CCR5TC
Plasmid#125691PurposeGlobal labeling of excitatory synapses (green fluorescence)DepositorInsertPSD95.FingR-eGFP-CCR5TC
UseAAVExpressionMammalianPromoterEF1AAvailable SinceJuly 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-Con/Foff 2.0-GCaMP6F
Plasmid#137123PurposeIntersectional viral expression of GCaMP6F in cells expressing Cre AND NOT FlpDepositorHas ServiceAAV8InsertCon/Foff 2.0-GCaMP6F
UseAAV, Cre/Lox, and Synthetic Biology ; Flp/frtMutationRemoved RSET tagPromoterEF1aAvailable SinceJune 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBS-EF1afull-H2B-mCherry-P2A-Fluc
Plasmid#207253PurposeLentiviral transfer genome encoding H2B-mCherry-P2A-Fluc driven by a full-length EF1a promoterDepositorInsertH2B-mCherry-P2A-Fluc
UseLentiviralExpressionMammalianPromoterEF1alpha_fullAvailable SinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-fDIO-eGFP-2A-Cre-WPRE
Plasmid#203842PurposeFlp-dependent Cre and EGFP expressionDepositorHas ServiceAAV8InsertCre
UseAAVTagsEGFP-2AAvailable SinceAug. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
mCherry-Cb5-IRES-puromycin-pLVx-EF1a
Plasmid#177434PurposeTo express mCherry fused to Cb5 endoplasmic reticulum (ER) targeting sequence. Lentiviral vector used to make cell lines expressing this ER landmark.DepositorAvailable SinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EF1alpha-eGFP-2xStrep-IRES-Puro
Plasmid#141395PurposeLentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirusDepositorInserteGFP
UseLentiviralTags2xStrepExpressionMammalianMutationhuman codon optimizedPromoterEF1alphaAvailable SinceApril 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-DIO-ChRmine-mScarlet-WPRE
Plasmid#130998PurposeDouble floxed ChRmine-mScarlet under the control of Ef1a promoterDepositorHas ServiceAAV1 and AAV5InsertChRmine
UseAAVTagsmScarletPromoterEf1aAvailable SinceOct. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSLQ5428_pHR_EF1a-mCherry-P2A-Rfx_Cas13d-2xNLS-3xFLAG
Plasmid#155305PurposeLentiviral vector encoding Rfx Cas13d fused with 2xNLS, 3xFLAG, and 2A-tagged mCherry.DepositorInsertmCherry 2A-tagged to Ruminococcus flavefaciens XPD3002 Cas13d
UseLentiviralTags2xNLS-3xFLAGExpressionMammalianPromoterEF1aAvailable SinceJuly 21, 2020AvailabilityAcademic Institutions and Nonprofits only