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169,450 results

Showing: 3441 - 3480 of 169450 results

  1. pET-pA-Hia5

    Plasmid
    #174372
    Purpose
    SEE DEPOSITOR COMMENTS BELOW. Plasmid for IPTG-inducible expression and purification of protein A-Hia5-6xHis (pA-Hia5) for antibody directed DNA (adenine) methylation (as in DiMeLo-seq)
    Depositor
    Aaron Straight
    Article
    Altemose et al Nat Methods. 2022 Jun;19(6):711-723. doi
    Insert
    protein A - hia5
    Tags
    6xHis and Protein A
    Expression
    Bacterial
    Available Since
    Sept. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    174372-plasmid-map-sequence-id-341860

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  3. MCP-MSN

    Plasmid
    #210704
    Purpose
    This plasmid express EF1a promoter driven MCP-MSN transactivation module
    Depositor
    Isaac Hilton
    Article
    Mahata et al Nat Methods. 2023 Oct 9. doi: 10.1038/s4
    Insert
    MCP fused to transactivation domain from Human transcription factor MRTF-A, STAT-1 and NRF2
    Use
    Lentiviral
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    N55K in MCP
    Promoter
    EF1a
    Available Since
    Nov. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    210704-plasmid-map-sequence-id-416247

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  4. eSpCas9(1.1)

    Plasmid
    #71814
    Purpose
    Expresses high specificity SpCas9. Px330-like plasmid.
    Depositor
    Feng Zhang
    Article
    Slaymaker et al Science. 2015 Dec 1. pii: aad5227.
    Has Service
    Cloning Grade DNA
    Insert
    enhanced specificity Cas9 (1.1)
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    K848A, K1003A, & R1060A
    Promoter
    CBh
    Available Since
    Dec. 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    71814-plasmid-map-sequence-id-177023

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  10. lenti-sgRNA hygro

    Plasmid
    #104991
    Purpose
    This 3rd generation lentiviral plasmid expresses a S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from a U6 promoter and hygromycin resistance from an EF-1a promoter.
    Depositor
    Brett Stringer
    Article
    Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Available Since
    Aug. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    104991-plasmid-map-sequence-id-335144

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  12. pAAV.CMV.HI.eGFP-Cre.WPRE.SV40 (AAV8)

    Viral Prep
    #105545-AAV8
    Purpose
    Ready-to-use AAV8 particles produced from pAAV.CMV.HI.eGFP-Cre.WPRE.SV40 (#105545). In addition to the viral particles, you will also receive purified pAAV.CMV.HI.eGFP-Cre.WPRE.SV40 plasmid DNA. CMV-driven EGFP-Cre expression. These AAV preparations are suitable purity for injection into animals.
    Depositor
    James M. Wilson
    Promoter
    HI
    Tags
    EGFP
    Available Since
    June 29, 2018
    Availability
    Academic Institutions and Nonprofits only

  20. pAAV-S5E2-ChR2-mCherry (AAV PHP.eB)

    Viral Prep
    #135634-PHPeB
    Purpose
    Ready-to-use AAV PHP.eB particles produced from pAAV-S5E2-ChR2-mCherry (#135634). In addition to the viral particles, you will also receive purified pAAV-S5E2-ChR2-mCherry plasmid DNA. Expression of ChR2-mCherry under the control of the E2 regulatory element. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Jordane Dimidschstein
    Article
    Vormstein-Schneider et al Nat Neurosci. 2020 Dec;23(12):1629-1636.
    Tags
    mCherry
    Available Since
    Oct. 28, 2021
    Availability
    Academic Institutions and Nonprofits only

  25. pC178: pAAV.U6.SapI.CMV.Cas13bt3

    Plasmid
    #204215
    Purpose
    Plasmid AAV vector expressing Cas13bt3 and hU6-driven expression of guide RNAs. Contains SapI sites for guide cloning flanked by 5' and 3' full-length DRs.
    Depositor
    Guei-Sheung Liu
    Article
    Kumar et al Proc Natl Acad Sci U S A. 2024 Nov 5;121
    Insert
    Cas13bt3 and U6.SapI sgRNA cloning site
    Use
    AAV and CRISPR
    Tags
    HA and NLS
    Expression
    Mammalian
    Mutation
    Codon optimisation by GenScript tool
    Promoter
    CMV/U6
    Available Since
    Dec. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    204215-plasmid-map-sequence-id-455913

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  26. pLKO.1 neo

    Plasmid
    #13425
    Purpose
    3rd gen lentiviral backbone for cloning and expression of new shRNA sequences. Uses neomycin for selection.
    Depositor
    Sheila Stewart
    Insert
    non-hairpin 18bp
    Use
    Lentiviral and RNAi
    Expression
    Mammalian
    Promoter
    U6 promoter
    Available Since
    Dec. 22, 2006
    Availability
    Academic Institutions and Nonprofits only
    13425-plasmid-map-sequence-id-360003

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  27. pLenti CMVtight eGFP Hygro (w783-1)

    Plasmid
    #26585
    Purpose
    2nd generation lentiviral vector
    Depositor
    Eric Campeau
    Insert
    Enhanced Green Fluorescent Protein
    Use
    Lentiviral; Tet-on advanced vector
    Expression
    Mammalian
    Available Since
    Jan. 6, 2011
    Availability
    Academic Institutions and Nonprofits only
    26585-plasmid-map-sequence-id-324283

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  28. mCherry-CD9-10

    Plasmid
    #55013
    Purpose
    Localization: Tetraspanin/Membrane, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Insert
    CD9 (CD9 Human)
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Aug. 29, 2014
    Availability
    Academic Institutions and Nonprofits only
    55013-plasmid-map-sequence-id-234553

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  29. pAAV-hSyn1-Flex-mRuby2-GSG-P2A-GCaMP6s-WPRE-pA (AAV1)

    Viral Prep
    #68720-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV-hSyn1-Flex-mRuby2-GSG-P2A-GCaMP6s-WPRE-pA (#68720). In addition to the viral particles, you will also receive purified pAAV-hSyn1-Flex-mRuby2-GSG-P2A-GCaMP6s-WPRE-pA plasmid DNA. Cre-dependent GCaMP6s calcium sensor and bicistronic, physically separate mRuby2 expression under a human synapsin1 promoter. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Tobias Bonhoeffer, Mark Huebener, Tobias Rose
    Article
    Rose et al Science. 2016 Jun 10;352(6291):1319-22.
    Promoter
    hSyn1-FLEX
    Tags
    mRuby2 (Cre-dependent)
    Available Since
    March 7, 2018
    Availability
    Academic Institutions and Nonprofits only

  30. MSCV IRES Luciferase

    Plasmid
    #18760
    Depositor
    Scott Lowe
    Type
    Empty backbone
    Use
    Luciferase and Retroviral
    Expression
    Mammalian
    Available Since
    July 3, 2008
    Availability
    Academic Institutions and Nonprofits only
    18760-plasmid-map-sequence-id-169127

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  31. pAAV-syn-iGluSnFR4f-NGR-WPRE

    Plasmid
    #234438
    Purpose
    AAV-mediated expression of glutamate sensor with improved sensitivity and fast deactivation kinetics (4f = fast); NGR vector matches or outperforms PDGFR in all conditions tested.
    Depositor
    GENIE Project
    Article
    Aggarwal et al bioRxiv 2025.03.20.643984
    Has Service
    AAV1
    Insert
    iGluSnFR4f-NGR
    Use
    AAV
    Tags
    IgK chain and Myc epi tag-NGR GPI anchor
    Expression
    Mammalian
    Promoter
    Synapsin
    Available Since
    March 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    234438-plasmid-map-sequence-id-465745

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  36. EGFP-pBAD

    Plasmid
    #54762
    Purpose
    Localization: Protein Expression Vector , Excitation: 488, Emission: 507
    Depositor
    Michael Davidson
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Tags
    6xHis and EGFP
    Expression
    Bacterial
    Available Since
    July 25, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    54762-plasmid-map-sequence-id-212808

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Showing: 3441 - 3480 of 169450 results