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175,655 results

Showing: 3441 - 3480 of 175655 results

  1. MSCV-IRES-GFP

    Plasmid
    #20672
    Purpose
    Depositor
    Tannishtha Reya
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Retroviral
    Tags
    IRES EGFP
    Expression
    Mammalian
    Available Since
    Aug. 28, 2009
    Availability
    Academic Institutions and Nonprofits only
    20672-plasmid-map-sequence-id-203241

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  6. pSL1521 (pSPIN, pSC101* backbone)

    Plasmid
    #160729
    Purpose
    Single-plasmid V. cholerae CAST system. Encodes all proteins, crRNA, donor DNA; non-targeting crRNA has BsaI sites for cloning. Temperature-sensitive pSC101* backbone can be cured by 37 °C incubation.
    Depositor
    Samuel H. Sternberg
    Article
    Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103
    Insert
    VchCAST proteins, crRNA, and donor DNA
    Expression
    Bacterial
    Promoter
    J23119
    Available Since
    Jan. 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    160729-plasmid-map-sequence-id-322926

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  11. pLPC-JAG1-mVenus

    Plasmid
    #171170
    Purpose
    Retroviral vector for CMV promoter driven expression of JAG1 (Notch pathway) fused to mVenus fluorescent protein (to be used in conjunction with Phoenix packaging cells).
    Depositor
    Masashi Narita
    Article
    Hoare et al Nat Cell Biol. 2016 Sep;18(9):979-92. do
    Insert
    JAG1 (JAG1 Human)
    Use
    Retroviral
    Tags
    mVenus
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    April 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    171170-plasmid-map-sequence-id-339658

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  12. N-Terminal Split Cas9 with GyrA intein

    Plasmid
    #58693
    Purpose
    Expresses truncated N-terminal SpCas9 domain fused to a GyrA intein, flanked by ITRs for AAV packaging. Combine with C-Terminal Split Cas9 Gyra Intein for full length SpCas9 production
    Depositor
    Gang Bao
    Article
    Fine et al Sci Rep. 2015 Jul 1;5:10777. doi: 10.103
    Insert
    Humanized N-Terminal S. pyogenes Cas9 with GyrA Nsplit Intein
    Use
    AAV and CRISPR
    Tags
    3xFlag, GyrA Nsplit Intein, and NLS
    Expression
    Mammalian
    Promoter
    CBh
    Available Since
    July 6, 2015
    Availability
    Academic Institutions and Nonprofits only
    58693-plasmid-map-sequence-id-492918

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  13. pAAV-EF1a-double floxed-mCherry-WPRE-HGHpA (AAV9)

    Viral Prep
    #20299-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pAAV-EF1a-double floxed-mCherry-WPRE-HGHpA (#20299). In addition to the viral particles, you will also receive purified pAAV-EF1a-double floxed-mCherry-WPRE-HGHpA plasmid DNA. EF1a-driven, Cre-dependent mCherry expression. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth
    Tags
    mCherry (Cre-dependent)
    Available Since
    Sept. 22, 2025
    Availability
    Academic Institutions and Nonprofits only

  19. pJW2098

    Plasmid
    #163094
    Purpose
    CRISPR repair template: add insertion site specific homology arms by PCR before insertion
    Depositor
    Jordan Ward
    Article
    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045
    Insert
    30aa linker::mScarlet (dpi)::AID*::3xFLAG::10aa linker
    Use
    CRISPR
    Expression
    Worm
    Mutation
    Silent mutations to remove piRNA sites
    Available Since
    Jan. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    163094-plasmid-map-sequence-id-320582

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  25. pLKO5.U6.DR130(CasRX)_Empty.EFS.tRFP657

    Plasmid
    #212961
    Purpose
    Lentiviral plasmid for the cloning of mature gRNAs of the RfxCas13d (CasRx) CRISPR system. As a marker, the plasmid also encodes the tRFP657 fluorescence protein.
    Depositor
    Roland Rad
    Article
    Montero et al Nat Methods. 2024 Feb 26. doi: 10.1038/s
    Insert
    DR1_CasRx_backbone
    Use
    CRISPR and Lentiviral
    Tags
    TagRFP657
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    Feb. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    212961-plasmid-map-sequence-id-422482

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  27. pVITRO1-Trastuzumab-IgG1/κ

    Plasmid
    #61883
    Purpose
    Expresses HER2/neu receptor specific humanized IgG1/κ antibody isotype (Trastuzumab-IgG1/κ)
    Depositor
    Andrew Beavil
    Article
    Dodev et al Sci Rep. 2014 Jul 30;4:5885. doi: 10.103
    Inserts
    HER2/neu receptor specific humanized Gamma 1 heavy chain expression cassette
    HER2/neu receptor specific humanized kappa light chain expression cassette
    Expression
    Mammalian
    Promoter
    mEF1 Prom and rEF1 Prom
    Available Since
    March 17, 2015
    Availability
    Academic Institutions and Nonprofits only
    61883-plasmid-map-sequence-id-358014

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  28. AAV-CAG-hChR2-H134R-tdTomato (AAV Retrograde)

    Viral Prep
    #28017-AAVrg
    Purpose
    Ready-to-use AAV Retrograde particles produced from AAV-CAG-hChR2-H134R-tdTomato (#28017). In addition to the viral particles, you will also receive purified AAV-CAG-hChR2-H134R-tdTomato plasmid DNA. Humanized channelrhodopsin H134R mutant fused to tdTomato, under the control of the CAG promoter. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karel Svoboda
    Article
    Mao et al Neuron. 2011 Oct 6;72(1):111-23.
    Promoter
    CAG
    Tags
    tdTomato
    Available Since
    June 20, 2017
    Availability
    Academic Institutions and Nonprofits only

  31. MoClo Tool Kit

    Plasmid Kit
    #1000000044
    Purpose
    Collection of standardized genetic modules that can be used for hierarchical assembly of multi-gene expression constructs.
    Depositor
    Sylvestre Marillonnet
    Application
    Cloning and Synthetic Biology
    Vector Type
    Eukaryotic Expression
    Cloning Type
    Golden Gate (MoClo)
    Available Since
    April 8, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  32. CMV-mCherry

    Plasmid
    #241313
    Purpose
    Expression of mCherry protein in mammalian cells
    Depositor
    Stavros Lomvardas
    Insert
    mCherry fluorescent protein
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Oct. 31, 2025
    Availability
    Academic Institutions and Nonprofits only
    241313-plasmid-map-sequence-id-480862

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  34. TFORF1974

    Plasmid
    #143988
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    TBX3 (TBX3 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Feb. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    143988-plasmid-map-sequence-id-385902

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  35. pOPARA2

    Plasmid
    #200417
    Purpose
    Golden Gate assembly of expression constructs containing pBAD promoter (spectinomycin resistance)
    Depositor
    Kamil Witek
    Article
    Lesley et al bioRxiv
    Type
    Empty backbone
    Expression
    Bacterial
    Promoter
    pBAD
    Available Since
    May 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    200417-plasmid-map-sequence-id-394144

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Showing: 3441 - 3480 of 175655 results