167,601 results

1. pcDNA3 APEX2-NES

   Plasmid

   #49386

   Purpose

   Expresses APEX2 in the cytosol of mammalian cells

   Depositor

   Alice Ting

   Article

   Lam et al Nat Methods. 2014 Nov 24. doi: 10.1038/n

   Insert

   Flag - APEX2 - NES
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   K14D, W41F, E112K, A134P on soybean APX; improved…

   Promoter

   CMV

   Available Since

   Nov. 27, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFA6a-link-yoEGFP-CaURA3

   Plasmid

   #44872

   Purpose

   Yeast tagging vector to create gene fusion with yeast optimized EGFP with CaUra3 selection

   Depositor

   Kurt Thorn, Wendell Lim

   Article

   Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

   Insert

   yoEGFP
   

   Expression

   Yeast

   Available Since

   May 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAGGS DRR mCherry Donor EF1a BFP

   Plasmid

   #98896

   Purpose

   Homologous recombination donor for pDRR. Expresses mCherry after repait by HR.

   Depositor

   Jan Karlseder

   Article

   Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

   Insert

   Neomycin-T2A-mCherry-GFP
   

   Use

   Synthetic Biology

   Expression

   Mammalian

   Available Since

   Nov. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. Arl13b-mcherry

   Plasmid

   #234845

   Purpose

   A mammalian expression plasmid encoding Arl13b with mcherry at C-terminus.

   Depositor

   Lei Lu

   Article

   Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

   Insert

   Arl13b (ARL13B Human)
   

   Expression

   Mammalian

   Available Since

   June 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV-Mm.cargo(Cre)

   Plasmid

   #174862

   Purpose

   Expresses mouse Peg10 cargoRNA encoding Cre recombinase

   Depositor

   Feng Zhang

   Article

   Segel et al Science. 2021 Aug 20;373(6557):882-889.

   Insert

   Cre flanked by MmPeg10 UTRs
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. ER-GCaMP6-210

   Plasmid

   #86919

   Purpose

   Fluorescent reporter for ER calcium signaling

   Depositor

   Timothy Ryan

   Article

   de Juan-Sanz et al Neuron. 2017 Jan 30. pii: S0896-6273(17)

   Insert

   ER-GCaMP6-210
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   K78H, T302L, R303P, A317E, D324G, D360G, D380Y, T…

   Promoter

   CAMKII

   Available Since

   March 15, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. RHO3

   Bacterial Strain

   #124700

   Purpose

   E. coli mobilizer strain that facilitates conjugation of mobilizable plasmids by using metabolic counter-selection against the donor strain.

   Depositor

   Herbert Schweizer

   Article

   Lopez et al Appl Environ Microbiol. 2009 Oct;75(20):

   Bacterial Resistance

   Dap (400 µg/ml)

   Available Since

   April 5, 2021

   Availability

   Academic Institutions and Nonprofits only

8. V5-GFP-TurboID-KDEL_pLX208

   Plasmid

   #202020

   Purpose

   expresses GFP-TurboID in the mammalian ER lumen, lentiviral vector

   Depositor

   Alice Ting

   Article

   Qin et al Cell. 2023 Jun 23:S0092-8674(23)00596-2.

   Insert

   V5-TurboID-KDEL
   

   Use

   Lentiviral

   Tags

   GFP and V5

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLS-mP

   Plasmid

   #81225

   Purpose

   Lentivirus enhancer assay plasmid that contains a minimal promoter (mP) and a EGFP reporter gene.

   Depositor

   Nadav Ahituv

   Article

   Inoue et al Genome Res. 2016 Nov 9. pii: gr.212092.1

   Type

   Empty backbone

   Use

   Lentiviral

   Promoter

   Derived from pGL4.23

   Available Since

   Jan. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. TurboID-EV

    Plasmid

    #215085

    Purpose

    Expresses TurboID in mammalian cells from a retroviral vector.

    Depositor

    Michele Pagano

    Article

    Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

    Insert

    TurboID (birA )
    

    Use

    Retroviral

    Expression

    Mammalian

    Promoter

    LTR

    Available Since

    April 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. gRNA NIA TLS2

    Plasmid

    #196981

    Purpose

    Expresses two gRNAs targeting Arabidopsis NIA1 gene for knockout. Each gRNA is fused to TLS2 mobility signal that confers graft mobility.

    Depositor

    Friedrich Kragler

    Article

    Yang et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    AtNIA1 gRNAs fused to TLS2 tags
    

    Expression

    Plant

    Promoter

    pU6-26, pU6-29

    Available Since

    May 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJRH-1362 scFv entry plasmid

    Plasmid

    #201912

    Purpose

    Entry vector for cloning single chain variable fragments displayed on the human CD8a hinge and transmembrane domain (CAG promoter)

    Depositor

    Jennifer Doudna

    Article

    Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

    Insert

    Stuffer sequence (drop out for scFv cloning) + CD8 hinge and transmembrane domain
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Aug. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAXY0002

    Plasmid

    #179835

    Purpose

    binary plant vector expressing eYGFPuv and GUS, both driven by a 35S promoter

    Depositor

    Xiaohan Yang

    Article

    Yuan et al Hortic Res. 2021 Nov 1;8(1):234. doi: 10

    Type

    Empty backbone

    Use

    Binary vector for plant transformation

    Expression

    Plant

    Available Since

    Nov. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET-dCas9-VP64-6xHis

    Plasmid

    #62935

    Purpose

    Expression of dCas9-VP64-6xHis in bacterial cells

    Depositor

    David Liu

    Article

    Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

    Insert

    dCas9-VP64
    

    Tags

    3xFLAG, 6xHis, NLS, and VP64

    Expression

    Bacterial

    Mutation

    D10A and H840A amino acid changes render Cas9 nuc…

    Promoter

    T7

    Available Since

    May 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV G-CEPIA1er

    Plasmid

    #58215

    Purpose

    Green fluorescent indicator for calcium imaging in the endoplasmic reticulum

    Depositor

    Masamitsu Iino

    Article

    Suzuki et al Nat Commun. 2014 Jun 13;5:4153. doi: 10.

    Insert

    G-CEPIA1er
    

    Tags

    myc

    Expression

    Mammalian

    Mutation

    G-GECO1.1 E299D M304L D328N N345K F360W G369S E37…

    Promoter

    CMV

    Available Since

    July 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDSW1728

    Plasmid

    #120812

    Purpose

    E. coli-C. difficile shuttle vector: tetracycline-inducible promoter (Ptet) driving expression of red fluorescent protein (mCherryOpt)

    Depositor

    David S. Weiss, Craig Ellermeier

    Article

    Ransom et al Appl Environ Microbiol. 2015 Mar;81(5):1

    Insert

    red fluorescent protein (mCherry), codon-optimized for C. difficile
    

    Expression

    Bacterial

    Promoter

    Ptet

    Available Since

    Feb. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pmCherry-MS2coatProtein

    Plasmid

    #103832

    Purpose

    Live cell reader for MS2 loop RNA production

    Depositor

    Karsten Rippe

    Article

    Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

    Insert

    mCherry-MS2 coat protein (cp )
    

    Expression

    Mammalian

    Mutation

    MCP: nt 202-240 are missing (13 aa), C242G (A81G)…

    Promoter

    CMV

    Available Since

    Jan. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. LV-pCMV-FLPo-pGK-mCl-GP33/66

    Plasmid

    #216481

    Purpose

    Expresses FLPo and mClover3 fluorescent protein with LMCV GP33-43 and GP66-77 epitopes embedded in loop of mclover to initiate immunogenic tumor cells in KPFrt mice

    Depositor

    Nikhil Joshi

    Article

    Cheung et al Cancer Cell (2025), doi: 10.1016/j.ccell

    Inserts

    FLPo Recombinase
    mClover3-GP33/66
    

    Use

    Lentiviral

    Promoter

    pCMV and pGK

    Available Since

    Oct. 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCHA-VRC01-scFv

    Plasmid

    #44874

    Depositor

    Dane Wittrup

    Article

    Mata-Fink et al J Mol Biol. 2013 Jan 23;425(2):444-56. d

    Insert

    VRC01 scFv
    

    Tags

    HA and Myc

    Expression

    Yeast

    Promoter

    GAL1

    Available Since

    July 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. GFP shRNA tet pLKO puro

    Plasmid

    #163017

    Purpose

    Control tet-inducible shRNA targeting eGFP

    Depositor

    Mark Rubin

    Article

    Cotter et al Mol Cancer Res. 2021 Jun 4. pii: 1541-77

    Insert

    Enhanced Green Fluorescent Protein
    

    Use

    Lentiviral

    Available Since

    Feb. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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21. pPGK-T7/2-CD44v8-10 (fl)

    Plasmid

    #137823

    Purpose

    expression of CD44 proteins in mammalian cells

    Depositor

    Ursula Gunthert

    Article

    Mielgo et al Cell Death Differ. 2006 Mar;13(3):465-77

    Insert

    CD44v8-10 (fl) (CD44 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    full length

    Promoter

    PGK

    Available Since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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22. MCS-TurboID MSCV-IRES-GFP

    Plasmid

    #207957

    Purpose

    Retroviral vector for cloning a gene of interest with a glycine-serine linker and TurboID on the C-terminus

    Depositor

    Timothy Ley

    Article

    Day et al J Clin Invest. 2023 Dec 7:e176311. doi:

    Insert

    TurboID
    

    Use

    Retroviral; Turboid biotin proximity ligase

    Tags

    TurboID

    Expression

    Mammalian

    Available Since

    Dec. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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23. pHIV-EF1a-2A-Ovalbumin(dN)-ZsGreen

    Plasmid

    #231986

    Purpose

    Empty backbone for expression of Ovalbumin with Bi-cistronic expression ZsGreen

    Depositor

    Brian Ruffell

    Article

    Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

    Insert

    OVA-2A-ZsGreen
    

    Use

    Lentiviral

    Available Since

    Feb. 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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24. UAS:mCherry

    Plasmid

    #170994

    Purpose

    UAS:GAL4 repeat-containing promoter drives simple mCherry reporter gene. Blasticidin selectable.

    Depositor

    Alice Ting

    Article

    Coukos et al eLife. 2021 Aug 20;10. pii: 69142. doi:

    Insert

    UAS:mCherry
    

    Use

    Lentiviral and Synthetic Biology

    Expression

    Mammalian

    Promoter

    Synthetic 14x UAS:Gal4

    Available Since

    Aug. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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25. pCLBW cox8 EGFP mCherry

    Plasmid

    #78520

    Purpose

    Fluorescent mitophagy reporter

    Depositor

    David Chan

    Article

    Rojansky et al Elife. 2016 Nov 17;5. pii: e17896. doi:

    Insert

    none
    

    Use

    Retroviral

    Tags

    cox8 EGFP mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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26. pAAV.hSyn.iGluSnFR3.v857.GPI (AAV1)

    Viral Prep

    #178331-AAV1

    Purpose

    Ready-to-use AAV1 particles produced from pAAV.hSyn.iGluSnFR3.v857.GPI (#178331). In addition to the viral particles, you will also receive purified pAAV.hSyn.iGluSnFR3.v857.GPI plasmid DNA. Synapsin-driven, third generation glutamate sensor iGluSnFR3, variant 857. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Kaspar Podgorski

    Article

    Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4

    Available Since

    May 16, 2023

    Availability

    Academic Institutions and Nonprofits only

27. pAAV-hSyn-DIO-rM3D(Gs)-mCherry (AAV8)

    Viral Prep

    #50458-AAV8

    Purpose

    Ready-to-use AAV8 particles produced from pAAV-hSyn-DIO-rM3D(Gs)-mCherry (#50458). In addition to the viral particles, you will also receive purified pAAV-hSyn-DIO-rM3D(Gs)-mCherry plasmid DNA. hSyn-driven, Cre-dependent rM3D(Gs) receptor with an mCherry reporter for chemogenetic excitation. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Bryan Roth

    Promoter

    Syn

    Tags

    mCherry (Cre-dependent)

    Available Since

    Jan. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

28. pEGFP-N1-PARP1-E988K

    Plasmid

    #211580

    Purpose

    CMV driven PARP1-E988K -eGFP expressing construct with IRES-Neomycin selection

    Depositor

    Chris Lord

    Article

    Krastev et al Nat Cell Biol. 2022 Jan;24(1):62-73. doi

    Insert

    PARP1 (PARP1 Human)
    

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Contains E988K mutation

    Available Since

    Feb. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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29. lenti-SpCas9 hygro

    Plasmid

    #104995

    Purpose

    This lentiviral construct delivers hSpCas9 and hygromycin resistance.

    Depositor

    Brett Stringer

    Article

    Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

    Insert

    KpnI-XhoI-BsrGI-NheI
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Aug. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. EGFP-Cyclin E1

    Plasmid

    #215053

    Purpose

    Expresses EGFP-Cyclin E1 in mammalian cells from a retroviral vector.

    Depositor

    Michele Pagano

    Article

    Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

    Insert

    CCNE1 (CCNE1 Human)
    

    Use

    Retroviral

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    LTR

    Available Since

    April 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pCMV-Hs.cargo(Cre)

    Plasmid

    #174863

    Purpose

    Expresses human Peg10 cargoRNA encoding Cre recombinase

    Depositor

    Feng Zhang

    Article

    Segel et al Science. 2021 Aug 20;373(6557):882-889.

    Insert

    Cre flanked by HsPeg10 UTRs
    

    Tags

    SV40 NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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32. 7M8

    Plasmid

    #64839

    Purpose

    This plasmid can be used in the triple transfection method of AAV vector production. It supplies the replication proteins from AAV2 and the 7M8 capsid protein.

    Depositor

    John Flannery, David Schaffer

    Article

    Dalkara et al Sci Transl Med. 2013 Jun 12;5(189):189ra

    Insert

    7M8 cap
    

    Use

    AAV

    Mutation

    7mer insertion in the AAV2 cap

    Available Since

    Aug. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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33. dCas13d-dsRBD

    Plasmid

    #154938

    Purpose

    BPNLS-dRfxCas13d-dsRBD-BPNLS-HA

    Depositor

    Alice Ting

    Article

    Han et al Proc Natl Acad Sci U S A. 2020 Aug 24. p

    Insert

    BPNLS-dRfxCas13d-dsRBD-BPNLS-HA
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pLminP_Luc2P_RE27

    Plasmid

    #90369

    Purpose

    Type II Interferon - STAT1/STAT1 gene reporter

    Depositor

    Ramnik Xavier

    Article

    O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

    Insert

    Firefly luciferase
    

    Use

    Lentiviral

    Promoter

    STAT1

    Available Since

    April 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. GFP-EEA1 wt

    Plasmid

    #42307

    Depositor

    Silvia Corvera

    Article

    Lawe et al J Biol Chem. 2000 Feb 4;275(5):3699-705.

    Insert

    EEA1 (EEA1 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available Since

    Jan. 31, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pAAV.Syn.Flex.NES-jRCaMP1b.WPRE.SV40 (AAV1)

    Viral Prep

    #100850-AAV1

    Purpose

    Ready-to-use AAV1 particles produced from pAAV.Syn.Flex.NES-jRCaMP1b.WPRE.SV40 (#100850). In addition to the viral particles, you will also receive purified pAAV.Syn.Flex.NES-jRCaMP1b.WPRE.SV40 plasmid DNA. Syn-driven, Cre-dependent NES-jRCaMP1b calcium sensor. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Douglas Kim, GENIE Project

    Article

    Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

    Promoter

    Syn

    Available Since

    May 11, 2018

    Availability

    Academic Institutions and Nonprofits only

37. pCBL101-RUBY

    Plasmid

    #199723

    Purpose

    Expresses betalain biosynthesis genes under CaMV 35S promoter

    Depositor

    Kan Wang

    Article

    Lee et al Plant Physiol. 2023 Apr 18:kiad231. doi:

    Insert

    35S-RUBY
    

    Expression

    Plant

    Available Since

    April 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. Nsp4-mCherry

    Plasmid

    #165132

    Purpose

    mammalian expression and localization

    Depositor

    Bruno Antonny

    Article

    Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

    Insert

    Nsp4 (ORF1ab codon optimized SARS-COV-2)
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pHR_Gal4UAS_dGFP_PGK_iRFP (pLZA042)

    Plasmid

    #203908

    Purpose

    Encodes dGFP downstream UAS promoter that's responsive to Gal4-VP64 activity in mammalian cells

    Depositor

    Jared Toettcher

    Article

    Zhu et al Nat Commun. 2023 Jun 2;14(1):3185. doi:

    Insert

    Gal4UAS_dGFP_PGK_iRFP
    

    Expression

    Mammalian

    Promoter

    5xUAS and PGK

    Available Since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pBABE-hygro-hTERT

    Plasmid

    #1773

    Purpose

    Retroviral expression of hTERT. Used to create immortalized cell lines.

    Depositor

    Bob Weinberg

    Article

    Counter et al Proc Natl Acad Sci U S A 1998 Dec 8;95(2

    Insert

    hTERT (TERT Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    July 18, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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