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We narrowed to 5,579 results for: PEP

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Showing: 4401 - 4450 of 5579 results

  3. MLNR-DuET

    Plasmid
    #213339
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    MLNR (MLNR Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213339-plasmid-map-sequence-id-430913

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  8. SSTR4-DuET

    Plasmid
    #213379
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    SSTR4 (SSTR4 Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213379-plasmid-map-sequence-id-430891

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  14. pYY1704

    Plasmid
    #198421
    Purpose
    pBs3:RedNTF:tNOS. A pGreen based plasmid expressing the INTACT reporter RedNTF after the promoter of the pepper Bs3 gene (pBs3). The plasmid has spectinomycin resistance.
    Depositor
    Yuan You
    Article
    You et al Nat Plants. 2023 Jan;9(1):128-141. doi:
    Insert
    RedNTF
    Expression
    Plant
    Promoter
    pBs3
    Available Since
    March 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    198421-plasmid-map-sequence-id-388243

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  16. MSP6-bio

    Plasmid
    #47732
    Purpose
    Expresses enzymatically monobiotinylated full-length MSP6 with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.
    Depositor
    Gavin Wright
    Article
    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.
    Insert
    Codon-optimised MSP6
    Tags
    enzymatic biotinylation sequence and rat Cd4 d3+4
    Expression
    Mammalian
    Mutation
    Exogenous signal peptide of mouse origin, changed…
    Promoter
    CMV
    Available Since
    Feb. 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    47732-plasmid-map-sequence-id-68561

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  17. MSP11-bio

    Plasmid
    #47734
    Purpose
    Expresses enzymatically monobiotinylated full-length MSP11 with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.
    Depositor
    Gavin Wright
    Article
    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.
    Insert
    Codon-optimised MSP11
    Tags
    enzymatic biotinylation sequence and rat Cd4 d3+4
    Expression
    Mammalian
    Mutation
    Exogenous signal peptide of mouse origin, changed…
    Promoter
    CMV
    Available Since
    Feb. 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    47734-plasmid-map-sequence-id-68746

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  18. AARP-bio

    Plasmid
    #47783
    Purpose
    Expresses enzymatically monobiotinylated full-length AARP ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.
    Depositor
    Gavin Wright
    Article
    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.
    Insert
    Codon-optimised AARP
    Tags
    enzymatic biotinylation sequence and rat Cd4 d3+4
    Expression
    Mammalian
    Mutation
    Exogenous signal peptide of mouse origin, changed…
    Promoter
    CMV
    Available Since
    Feb. 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    47783-plasmid-map-sequence-id-68815

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  19. MSP4-bio

    Plasmid
    #47717
    Purpose
    Expresses enzymatically monobiotinylated full-length MSP4 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.
    Depositor
    Gavin Wright
    Article
    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.
    Insert
    Codon-optimised MSP4
    Tags
    enzymatic biotinylation sequence and rat Cd4 d3+4
    Expression
    Mammalian
    Mutation
    Exogenous signal peptide of mouse origin, changed…
    Promoter
    CMV
    Available Since
    Feb. 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    47717-plasmid-map-sequence-id-68456

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  20. pExpreS2-1-C3-10H-SIII

    Plasmid
    #175444
    Purpose
    HiFi-compatible destination vector for secreted overexpression of 3C-cleavable, C-terminal 10His-TwinStrep tagged proteins with ExpreS2 Platform
    Depositor
    Wyatt Yue
    Article
    Coker et al Front Bioeng Biotechnol. 2022 May 5;10:8
    Type
    Empty backbone
    Tags
    3C-10His-TwinStrep and BiP Secretion Peptide
    Expression
    Insect
    Promoter
    Fused Actin-HSP70
    Available Since
    March 30, 2022
    Availability
    Academic Institutions and Nonprofits only
    175444-plasmid-map-sequence-id-358135

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  23. pET28-AviTag-DogTag-MBP

    Plasmid
    #171773
    Purpose
    Expresses AviTag-DogTag-Maltose binding protein in bacterial cytoplasm. The AviTag enables site-specific biotinylation by BirA.
    Depositor
    Mark Howarth
    Article
    Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-
    Insert
    AviTag-DogTag-MBP
    Tags
    His6 and Thrombin cleavage site
    Expression
    Bacterial
    Mutation
    Peptide tag added to N-terminus of MBP via a GSGE…
    Promoter
    T7
    Available Since
    July 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    171773-plasmid-map-sequence-id-338517

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  26. pcDNA3-Akt-STOPS

    Plasmid
    #175006
    Purpose
    Akt Substrate-based Tandem Occupancy Peptide Sponge. Genetically encoded for perturbing cellular Akt kinase activity.
    Depositor
    Jin Zhang
    Article
    Zhou et al Nat Commun. 2020 Nov 30;11(1):6088. doi:
    Insert
    mCherry-Akt-STOPS
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    175006-plasmid-map-sequence-id-344970

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  28. MRGPRD-DuET

    Plasmid
    #213340
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    MRGPRD (MRGPRD Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213340-plasmid-map-sequence-id-430868

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  29. pExpreS2-1-C3-PA-10H-SIII

    Plasmid
    #175443
    Purpose
    HiFi-compatible destination vector for secreted overexpression of 3C-cleavable, C-terminal Protein A-10His-TwinStrep tagged proteins with ExpreS2 Platform
    Depositor
    Wyatt Yue
    Article
    Coker et al Front Bioeng Biotechnol. 2022 May 5;10:8
    Type
    Empty backbone
    Tags
    3C-Protein A-10His-TwinStrep and BiP Secretion Pe…
    Expression
    Insect
    Promoter
    Fused Actin-HSP70
    Available Since
    April 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    175443-plasmid-map-sequence-id-360135

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  31. pQCXIP-mCherry-CDMPR

    Plasmid
    #182643
    Purpose
    Mammalian expression vector encoding a protein fusion composed of an N-terminal ER targeting sequence of hemagglutinin (HA), followed by mCherry and murine CDMPR (without its signal peptide)
    Depositor
    Martin Spiess
    Article
    Buser et al Life Sci Alliance. 2022 Mar 21;5(7). pii
    Insert
    CDMPR
    Use
    Retroviral
    Tags
    N-terminal ER targeting sequence of hemagglutinin…
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    April 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    182643-plasmid-map-sequence-id-358629

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  32. pTet-On-TAP

    Plasmid
    #59017
    Purpose
    Construct allows tetracycline inducible expression of a protein of interest fused to a tandem affinity purification tag (TAP) in toxoplasma gondii.
    Depositor
    David Sibley
    Article
    Etheridge et al Cell Host Microbe. 2014 May 14;15(5):537
    Type
    Empty backbone
    Use
    Toxoplasma expression
    Tags
    Calmodulin Binding Peptide fused with a TEV Cleav…
    Promoter
    RPS13 with Tet Operators Inserted
    Available Since
    Sept. 29, 2014
    Availability
    Academic Institutions and Nonprofits only
    59017-plasmid-map-sequence-id-423220

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  33. pME-H2B-mCerulean3-P2A-mKOFP2-CAAX

    Plasmid
    #75154
    Purpose
    Nuclear mCerulean2 (blue) fluorophore and a membrane targeted KOFP2 (orange) fluorophore separated by a self-cleaving 2A peptide for use with 5’ promoters.
    Depositor
    Nicholas Cole
    Article
    Don et al Zebrafish. 2016 Sep 15.
    Insert
    H2B-mCerulean3-P2A-mKOFP2-CAAX
    Use
    Middle entry vector for multisite gateway three f…
    Promoter
    n/a
    Available Since
    Oct. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    75154-plasmid-map-sequence-id-142108

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  34. SSTR3-DuET

    Plasmid
    #213378
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    SSTR3 (SSTR3 Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213378-plasmid-map-sequence-id-430734

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  36. pCEP4-DU422gp140-TM

    Plasmid
    #123251
    Purpose
    Mammalian expression plasmid for soluble gp140 from the DU422 HIV-1 isolate with a transmembrane tether
    Depositor
    Erik Procko
    Article
    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.
    Insert
    HIV-1 (DU422) Env
    Tags
    CD5 leader peptide and Linker (with 6his tag) and…
    Expression
    Mammalian
    Mutation
    Codon-optimized synthetic gene
    Promoter
    CMV
    Available Since
    April 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    123251-plasmid-map-sequence-id-235207

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  40. OPN3-DuET

    Plasmid
    #213357
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    OPN3 (OPN3 Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213357-plasmid-map-sequence-id-430879

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  43. pGEM-NLS-Cre-2A-mCherry-FKF

    Plasmid
    #119868
    Purpose
    Donor cassette containing NLS-tagged Cre, a Thosea asigna virus peptide (2A), mCherry and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection gene
    Depositor
    Tatjana Sauka-Spengler
    Article
    Weinberger et al Dev Cell. 2020 Mar 9;52(5):574-590.e6. d
    Insert
    NLS-Cre, 2A, mCherry and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene
    Tags
    mCherry
    Expression
    Bacterial
    Available Since
    April 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    119868-plasmid-map-sequence-id-240979

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  47. pQCXIP-EGFP-CDMPR

    Plasmid
    #182642
    Purpose
    Mammalian expression vector encoding a protein fusion composed of an N-terminal ER targeting sequence of hemagglutinin (HA), followed by EGFP and murine CDMPR (without its signal peptide)
    Depositor
    Martin Spiess
    Article
    Buser et al Life Sci Alliance. 2022 Mar 21;5(7). pii
    Insert
    CDMPR
    Use
    Retroviral
    Tags
    EGFP and N-terminal ER targeting sequence of hema…
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    April 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    182642-plasmid-map-sequence-id-358624

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  48. OPRD1-DuET

    Plasmid
    #213358
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    OPRD1 (OPRD1 Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213358-plasmid-map-sequence-id-430880

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  50. UTS2R-DuET

    Plasmid
    #213386
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    UTS2R (UTS2R Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213386-plasmid-map-sequence-id-430893

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Showing: 4401 - 4450 of 5579 results