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Plasmid#127458PurposeEntry plasmid for guide cloningDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJuly 23, 2019AvailabilityAcademic Institutions and Nonprofits only
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pcDNA5/FRT/TO_CalR_HaloTag9_KDEL
Plasmid#175540PurposeExpression of HaloTag9 in the endoplasmid reticulum of mammalian cellsDepositorInsertCalR-HaloTag9-KDEL
UseTagsExpressionMammalianMutationHaloTag9 = HaloTag7-Q165H-P174RPromoterAvailable sinceDec. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-S5E2-GCaMP6f (AAV9)
Viral Prep#135632-AAV9PurposeReady-to-use AAV9 particles produced from pAAV-S5E2-GCaMP6f (#135632). In addition to the viral particles, you will also receive purified pAAV-S5E2-GCaMP6f plasmid DNA. Expression of GCaMP6f under the control of the E2 regulatory element. These AAV preparations are suitable purity for injection into animals.DepositorPromoterTagsNoneAvailable sinceSept. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EF1a-EGFP-RAB7A-IRES-Puromycin
Plasmid#133027PurposeExpresses EGFP-tagged late endosome markerDepositorInsertRAB7A (RAB7A Human)
UseLentiviralTagsEGFPExpressionMammalianMutationPromoterHuman elongation factor 1 alphaAvailable sinceFeb. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET_mNG2(1-10)_32aalinker_mNG2(11)
Plasmid#82611PurposeBacterial expression vector for split mNeonGreen2 screeningDepositorInsertmNeonGreen2(1-10)_32aalinker_mNeonGreen2(11)
UseTagsExpressionBacterialMutationsplit before mutationsPromoterT7Available sinceMay 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Frankenbody-FLAG-mEGFP
Plasmid#175293PurposeTrack mature and nascent FLAG tagged proteins in live cellsDepositorInsertanti-FLAG frankenbody fused with mEGFP
UseTagsmEGFPExpressionMammalianMutationPromoterAvailable sinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
AID* Kit
Plasmid Kit#1000000120PurposeExpanded tool kit for the auxin-inducible degron (AID) system in yeast ( S. cerevisiae). Includes tags for easy detection by antibodies/microscopy and a set of selection markers.DepositorAvailable sinceJan. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-GINKO1
Plasmid#113112PurposeMammalian expression of genetically encoded green fluorescent potassium ion indicator GINKO1DepositorInsertGINKO1
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pX330-PITCh-H2BC11
Plasmid#207755PurposeExpresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of H2BC11 for knock-in.DepositorInsertsgRNA Targeting C-terminus of H2BC11 (H2BC11 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceDec. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
kiCAP-AAV-BI-hTFR1
Plasmid#218796PurposeRepCap for AAV productionDepositorInsertAAV Rep-Cap BI-hTFR1
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
Lentiviral-S-tdTomato
Plasmid#112579PurposeExpressing Shuttle-tdTomato, or NLS-NES-tagged tdTomato in a Lentiviral vectorDepositorInsertNLS-tdTomato-NES
UseLentiviralTagsnuclear export sequence and nuclear localization …ExpressionMammalianMutationPromoterAvailable sinceJuly 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBS-CMV-gagpol
Plasmid#35614DepositorInsertMLV gag and pol genes
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 12, 2012AvailabilityAcademic Institutions and Nonprofits only -
pACYCDuet-1_Ec_rpoZ
Plasmid#128837PurposeE. coli rpoz gene cloned between NcoI and HindIII sites of the first MCS of this Duet plasmid. Omega protein.DepositorInsertrpoZ (rpoZ E. coli)
UseTagsExpressionBacterialMutationPromoterT7Available sinceAug. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
APEX2-csGBP
Plasmid#108874PurposeExpression of APEX2 fused to a conditionally stable nanobody against GFPDepositorInsertAPEX2-csGBP
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pENN.AAV.cTNT.PI.eGFP.WPRE.rBG (AAV9)
Viral Prep#105543-AAV9PurposeReady-to-use AAV9 particles produced from pENN.AAV.cTNT.PI.eGFP.WPRE.rBG (#105543). In addition to the viral particles, you will also receive purified pENN.AAV.cTNT.PI.eGFP.WPRE.rBG plasmid DNA. cTNT-driven eGFP. These AAV preparations are suitable purity for injection into animals.DepositorPromotercTNTTagsEGFPAvailable sinceJuly 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET StrepII TEV LIC cloning vector (1R)
Plasmid#29664DepositorTypeEmpty backboneUseTagsStrep II and TEV cleavage siteExpressionBacterialMutationPromoterT7-lacO (lactose/IPTG inducible)Available sinceJune 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
LAMP1-RpHLuorin2
Plasmid#171720PurposeLysosomal expression of ratiometric pHLuorin2DepositorInsertRatiometric pHLuorin2 fused to LAMP1 (LAMP1 Human)
UseTagsratiometric pHluorin2ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
FUW-OSKM
Plasmid#20328PurposeLentiviral plasmid expressing mouse Oct4, Sox2, Klf4 and cMyc for iPS cell generationDepositorUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 23, 2009AvailabilityAcademic Institutions and Nonprofits only -
TRE-UBC-hCD47-T2A-GFP
Plasmid#205458Purposelentiviral plasmid for expression of human CD47DepositorInserthCD47 (CD47 Human)
UseTagsExpressionMammalianMutationPromoterUBCAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
Integrin β3-GFP
Plasmid#205091PurposeExpress GFP-tagged human integrin β3 in mammalian cellsDepositorInsertIntegrin beta 3 (ITGB3 Human)
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceSept. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
AAV-hSyn-ASAP4e-Kv-WPRE
Plasmid#201031PurposeExpresses somatically enriched ASAP4e in neurons, can be used to package AAVDepositorInsertASAP4e-Kv
UseAAVTagsExpressionMutationPromoterAvailable sinceJuly 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-S5E2-dTom-nlsdTom (AAV9)
Viral Prep#135630-AAV9PurposeReady-to-use AAV9 particles produced from pAAV-S5E2-dTom-nlsdTom (#135630). In addition to the viral particles, you will also receive purified pAAV-S5E2-dTom-nlsdTom plasmid DNA. Bicistronic expression of dTomato and nuclear-targeted dTomato under the control of the E2 regulatory element. These AAV preparations are suitable purity for injection into animals.DepositorPromoterTagsdTomatoAvailable sinceNov. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
SpyCatcher003-sfGFP
Plasmid#133449PurposeExpresses SpyCatcher003-superfolder GFP in bacterial cytoplasmDepositorInsertSpyCatcher003-sfGFP
UseTagsHis6 and TEV siteExpressionBacterialMutationPromoterT5Available sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
PB_tre_dCas9_KRAB
Plasmid#126030PurposePiggyBac cargo vector expressing doxycycline inducible dCas9-KRAB fusionDepositorInsertsdCas9 fusion with KRAB
Hygromycin resistance
UseCRISPR; PiggybacTagsExpressionMammalianMutationD10A, H840A (catalytically inactive Cas9)PromoterEF1 alpha and TREAvailable sinceJune 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEM-3z/601
Plasmid#26656DepositorInsert
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 2, 2010AvailabilityAcademic Institutions and Nonprofits only -
VSV.G
Plasmid#14888DepositorInsertVSV-G Envelope
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
SrtA-PDGFR Tm
Plasmid#121168PurposepMP71 vector expressing Tomato - P2A - flag - SrtA - PDGFR TmDepositorInsertSrtA-PDGFR
UseRetroviralTagsTomato and flagExpressionMammalianMutationPromoterAvailable sinceFeb. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pNS35 (multiAsCas12a piggyBac)
Plasmid#217336PurposepiggyBac expression of multiAsCas12aDepositorInsertmultiAsCas12a
UseCRISPRTags6xMycNLS and HA-SV40NLS-P2A-TagBFP2ExpressionMammalianMutationR1226A/E174R/S542R/K548RPromoterCAGAvailable sinceMarch 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
PB-TO-ASCL1-DLX2
Plasmid#182307PurposePiggybac Tet-ON plasmid for differentiating hiPSCs into GABAergic neuron via ASCL1 and DLX2 expressionUseTagsNone and T2A-mycNLS-mTagBFP2ExpressionMammalianMutationPromoterEF1a and pTRE3G-bidirectionalAvailable sinceApril 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
MP1
Plasmid#69627PurposeUntargetted Bacterial MutagenesisDepositorInsertdnaQ926 umuD' umuC recA730
UseTagsExpressionBacterialMutationPromoterE. coli PBADAvailable sinceSept. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 236HSC WT (BRCA2)
Plasmid#16246DepositorInsertBRCA2 (BRCA2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 30, 2007AvailabilityAcademic Institutions and Nonprofits only -
p8.91
Plasmid#187441PurposeLentiviral packaging plasmid expressing gag and pol.DepositorInsertsUseLentiviralTagsExpressionMutationPromoterAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pET/ELP-I-CAT
Plasmid#71461PurposeELP-intein purification systemDepositorTypeEmpty backboneUseTagsELP ((VPGXG)110) and inteinExpressionBacterialMutationPromoterAvailable sinceApril 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-FGFR3-S249C
Plasmid#116386PurposeLentiviral expression of FGFR3 S249CDepositorInsertFGFR3 (FGFR3 Human)
UseLentiviralTagsExpressionMutationS249CPromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-DIO-hM3D(Gq)-mCherry (AAV8)
Viral Prep#44361-AAV8PurposeReady-to-use AAV8 particles produced from pAAV-hSyn-DIO-hM3D(Gq)-mCherry (#44361). In addition to the viral particles, you will also receive purified pAAV-hSyn-DIO-hM3D(Gq)-mCherry plasmid DNA. Syn-driven, Cre-dependent, hM3D(Gq) receptor with an mCherry reporter for CNO-induced neuronal activation. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsmCherry (Cre-dependent)Available sinceSept. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCH49 (crRNA expression vector)
Plasmid#217338PurposeCROP-seq crRNA expression vector with GFP and neo markerDepositorInsertAsDR-filler-AsDR8, GFP-neo
UseCRISPR and LentiviralTagsExpressionMutationPromoterhU6Available sinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
gCH128 (non-targeting crRNA)
Plasmid#217339PurposeNon-targeting crRNADepositorInsertcrNT-3
UseCRISPR and LentiviralTagsExpressionMutationPromoterhU6Available sinceMarch 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
5xUAS:GAP-mCherry-P2A-NfsB_Vv F70A/F108Y,he:tagBFP2
Plasmid#158653Purposemaking transgenics expressing a UAS driven mCherry and NTR 2.0DepositorInsert5xUAS:GAP-mCherry-P2A-NfsB_Vv F70A/F108Y;he:BFP
UseTol2-based transgenic expression in zebrafish/oth…TagsExpressionMutationF70A/F108YPromoterAvailable sinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-fDIO-ChrimsonR-tdTomato (AAV9)
Viral Prep#171027-AAV9PurposeReady-to-use AAV9 particles produced from pAAV-Ef1a-fDIO-ChrimsonR-tdTomato (#171027). In addition to the viral particles, you will also receive purified pAAV-Ef1a-fDIO-ChrimsonR-tdTomato plasmid DNA. Flp-dependent, Ef1a-driven expression of ChrimsonR-tdTomato fusion for optogenetic activation. These AAV preparations are suitable purity for injection into animals.DepositorPromoterEF1aTagstdTomatoAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only