We narrowed to 5,933 results for: chia
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Plasmid#79050PurposeExpress wild type AlkB protein in E. coli BL21(DE3)DepositorInsertAlkB (alkB E.coli)
TagsHis tagExpressionBacterialMutationA truncated AlkB with deletion of the amino (N)-t…PromoterT7Available SinceAug. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET21-Streptavidin-K121R
Plasmid#89880PurposeStreptavidin that has unimpaired biotin binding after dye labelingDepositorInsertStreptavidin-K121R-Glutamate_Tag
Tags6 glutamate tag (C terminal on insert)ExpressionBacterialMutationK121RAvailable SinceAug. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBbA5c-MevT-MBIS
Plasmid#35150DepositorInsertAtoB(o)-HMGS(o)-HMGR(o)-MK(o)-PMK(o)-PMD-Idi-IspA
UseSynthetic BiologyExpressionBacterialPromoterLacUV5,Available SinceMarch 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
pIRE4-Bpa
Plasmid#155342PurposePlasmid encoding the translational pair that enables efficient incorporation of Benzoyl-phenylalanine in response to the amber codon into proteins in mammmalian cells.DepositorInserthumanized EBpaRS/BstYam
ExpressionMammalianPromoterPGK/U6Available SinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTP264
Plasmid#149334PurposeBacterial expression of Biotin ligase BirADepositorInsertBiotin ligase BirA
TagsHis14 and bdNEDD8ExpressionBacterialAvailable SinceJune 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-MCN-10His-mStayGold (E138D)
Plasmid#211361PurposeBacterial expression of the bright and photostable monomeric StayGold fluorescent protein (E138D). Codon optimised for expression in Escherichia coli. Contains a N-terminal 10xHis tag.DepositorInsertmStayGold
Tags10xHis-tagExpressionBacterialMutationE138DPromoterT7Available SinceDec. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 mycBioID
Plasmid#35700PurposeTo fuse your protein of interest to the C-terminus of BirA(R118G) and use in proximity-dependent biotin identification (BioID); myc tagDepositorHas ServiceCloning Grade DNAInsertBirA
TagsMycExpressionMammalianMutationR118G (highly promiscuous form)PromotercmvAvailable SinceApril 5, 2012AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 MCS-BirA(R118G)-HA
Plasmid#36047PurposeTo fuse your protein of interest to the N-terminus of BirA(R118G) and use in proximity-dependent biotin identification (BioID); HA tagDepositorHas ServiceCloning Grade DNAInsertBirA(R118G)-HA
TagsHAExpressionMammalianMutationR118G (highly promiscuous form)Available SinceApril 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCT5-bac2.0
Plasmid#119872PurposeCumate-inducible expression of sfGFP gene in Bacillus subtilis, B. megaterium, and Escherichia coliDepositorInsertsCymR
sfGFP
UseSynthetic BiologyExpressionBacterialPromoterPveg promoter with CuO sequence and PxylR promoterAvailable SinceJan. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSLS.400
Plasmid#187201PurposeExpresses retron Eco4 RTDepositorInsertRetron Eco4 Reverse Transcriptase
UseSynthetic BiologyExpressionBacterialPromotermphRAvailable SinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET15bpATnp
Plasmid#121137PurposeThis vector encode a His6-tagged proteinA-Tnp(hyperactive form) fusion protein.DepositorInsertProtein A-Tnp
TagshisExpressionBacterialPromoterT7Available SinceMarch 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEF1a-BirA-V5-neo
Plasmid#100548PurposeExpresses C-terminal V5-tagged BirA biotin ligaseDepositorInsertC-terminal V5-tagged BirA
TagsV5, HisExpressionMammalianPromoterHuman EF1aAvailable SinceSept. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pNP566
Plasmid#222724PurposepNP561 - IS621 WT Target with T7 Inducible IS621 RecombinaseDepositorInsertIS621 Recombinase
ExpressionBacterialAvailable SinceJuly 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMD1 anti-GFP nanobody-Tn5
Plasmid#215567PurposeExpresses anti-GFP nanobody-Tn5 fusion in bacteria with an N-terminal 3xFlag tag and a C-terminal intein-CBD-intein tagDepositorInsertanti-GFP nanobody-Tn5 fusion
Tags3xFlag and intein-CBDExpressionBacterialPromoterT7Available SinceNov. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCT5-bac1.8
Plasmid#119871PurposeConstitutive expression of sfGFP gene in Bacillus subtilis, B. megaterium, and Escherichia coliDepositorInsertsCymR'
sfGFP
UseSynthetic BiologyExpressionBacterialMutationSNP in 592 bpPromoterPserA promoter and Pveg promoter with CuO sequenceAvailable SinceJan. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSIM24-GUS
Plasmid#67882PurposePlant gene expression vector pSIM24 containing the GUS (Beta-glucuronidase) reporter geneDepositorInsertuidA
ExpressionPlantMutationT120APromoterM24Available SinceAug. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
Ecoli PancACe
Plasmid#215712PurposeExpresses acetyl-CoA biosensor in EcoliDepositorInsertPancACe
Tags6xHisExpressionBacterialPromoterT7Available SinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBES1-LacOx50-Hyg
Plasmid#210261PurposeIntegrates 50xLacO repeats upstream of the BES1 promoter with a hygromycin drug selection marker downstream of the promoter.DepositorInsert50xLacO repeats derived from: PMID: 12864855.
Available SinceNov. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pOxyR_gfp
Plasmid#194154PurposeGreen Fluorescent Reporter of intracellular hydrogen peroxideDepositorInserttranscriptional regulator, OxyR, cognate promoter PoxyS, fluorescent protein sfGFP
ExpressionBacterialPromoterPoxyR, PoxySAvailable SinceFeb. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
ABE8r-R153del
Plasmid#208296PurposeExpresses ABE8r in mammalian cellsDepositorInsertTadA8r-R153del-SpCas9 D10A
ExpressionMammalianMutationW23R, H36L, R47K, P48A, R51L, I76Y, V82S, A106V, …Available SinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNP1954
Plasmid#222730PurposepNP1723 - IS621 WT Donor and bridgeRNA in trans (TBL-T5, DBL-WT)DepositorInsertIS621 bridge RNA
TagssfGFPExpressionBacterialAvailable SinceAug. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
p201N Cas9
Plasmid#59175PurposeCas9 driven by double 35S, nptII for plant selection, I-PpoI site to accept gRNA from pUC gRNA ShuttleDepositorInsertsCas9
nptII
UseCRISPRPromoter2x35S and StUbi-3PAvailable SinceSept. 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
pXD70LacZ1-promoterless-LacZ
Plasmid#191617PurposeE. coli - Eggerthella lenta shuttle plasmid (KanR), promoterless-lacZDepositorInsertlacZ
ExpressionBacterialAvailable SinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28a_T5-ARG1
Plasmid#106476PurposeExpresses acoustic reporter genes or gas vesicles (ARG1) in Escherichia coli Nissle 1917DepositorInsertARG1
UseSynthetic BiologyExpressionBacterialPromoterT5Available SinceMarch 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
SpyTag-β-Lactamase-SpyCatcher
Plasmid#52656PurposeExpresses SpyTag-SpyCatcher mediated cyclized version of TEM-1 β-lactamase (BLA). Cyclization of BLA led to an increase in thermal resilience of >60 degrees celsius.DepositorInsertTEM-1 β-Lactamase
TagsSpyCatcher and SpyTagExpressionBacterialPromoterT7 promoterAvailable SinceMay 1, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBES1-LacOx50-mCh-bla
Plasmid#210260PurposeIntegrates 50xLacO repeats and an mCherry gene upstream of the BES1 promoter with a hygromycin drug selection marker downstream of the promoter.DepositorInserts50xLacO repeats derived from: PMID: 12864855.
mCherry
Available SinceNov. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pNP568
Plasmid#222725PurposepNP555 - IS621 RE-LE TBL-WT, DBL-WT bRNA in cisDepositorInsertIS621 bridge RNA
TagssfGFPExpressionBacterialAvailable SinceJuly 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
ABE8r
Plasmid#208294PurposeExpresses ABE8r in mammalian cellsDepositorInsertTadA8r-SpCas9 D10A
ExpressionMammalianMutationW23R, H36L, R47K, P48A, R51L, I76Y, V82S, A106V, …Available SinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBMN YFP-DHFR(DD)
Plasmid#29326DepositorInsertYFP-DHFR(DD)
UseRetroviralTagsHA and YFPMutationChange Arg 12 to His (R12H),Asn 18 Thr (N18T), Va…Available SinceApril 1, 2011AvailabilityAcademic Institutions and Nonprofits only -
Dead-Laconic/pcDNA3.1(-)
Plasmid#118627PurposeTo express in mammalian cells a lactate FRET sensor negative control.DepositorInsertDead-Laconic
ExpressionMammalianMutationChange Histidine 151 to Aspartic Acid of Escheric…PromoterCMV PromoterAvailable SinceDec. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
ABE8r-V106W
Plasmid#208295PurposeExpresses ABE8r in mammalian cellsDepositorInsertTadA8r-V106W-SpCas9 D10A
ExpressionMammalianMutationW23R, H36L, R47K, P48A, R51L, I76Y, V82S, A106W, …Available SinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAR357_SFP_pCDF-1b
Plasmid#122851PurposeCo-expresses the phosphopantheteine transferase Sfp of Bacillus subtilis to transfer a PPant arm to acyl carrier proteins in Escherichia coli. Untagged; in pCDF-1b vector.DepositorInsertSfp
ExpressionBacterialPromoterT7 promoterAvailable SinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRT801
Plasmid#68112PurposeIntegration vector derived from Streptomyces phage phiBT1. Apramycin resistance gene, oriT, ColE1 origin of replication.DepositorInsertsaac3(IV)
oriT
attP
int
UseStreptomyces integrating vectorAvailable SinceJuly 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJUMP24_T24_pRham-ilvA-relE
Plasmid#201531PurposeExpressing synthetic overlapping gene, ilvA-relE. Origin pRO1600/ColE1 (E.coli - Pseudomonas shuttle)DepositorInsertilvA-relE
UseSynthetic BiologyPromoterPrhaBAD (rhamnose)Available SinceJune 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
G555
Plasmid#216483PurposeExpression of PlsB, PlsC, CdsA and PssA under the control of T7 promoters. The 4-gene insert is flanked by Phi29 replication origins.DepositorInsertplsB, plsC, cdsA, pssA
UseCell-free expressionPromoterT7 for each geneAvailable SinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pOZITX-S1
Plasmid#184925PurposeExpresses the catalytic S1 subunit of OZITX (EcPltAB)DepositorInsertcodon optimized S1 subunit of OZITX
ExpressionMammalianAvailable SinceMay 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSR83
Plasmid#90211PurposeExpresses the response regulator OmpR, and expresses mCherry from the Cph8-OmpR regulated PompF-146 promoterDepositorAvailable SinceMay 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
pOxyR_rfp
Plasmid#194155PurposeRed Fluorescent Reporter of intracellular hydrogen peroxideDepositorInserttranscriptional regulator, OxyR, cognate promoter PoxyS, fluorescent protein mCherry
ExpressionBacterialPromoterPoxyR, PoxySAvailable SinceFeb. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pNP1830
Plasmid#222728PurposepNP561 - T5 Target with T7 Inducible IS621 RecombinaseDepositorInsertIS621 Recombinase
ExpressionBacterialAvailable SinceJuly 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMA2640
Plasmid#25434PurposeRetroviral Tet-On vector for CMV-driven rtTA with EGFP and Blasticidin selectionDepositorInsertreverse tetracycline-regulated transactivator Advanced
UseRetroviralExpressionMammalianPromoterCMVAvailable SinceJune 21, 2010AvailabilityAcademic Institutions and Nonprofits only -
pSLS.492
Plasmid#184957PurposeTest effect of a1/a2 length on editing rpoB using retron recombineeringDepositorInsertEco1 RT and recombineering ncRNA, rpoB T1534C, a1/a2 length: 22
ExpressionBacterialMutationrpoB donor T1534C, a1/a2 length extended to 22 bpPromoterT7/lacAvailable SinceNov. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-MCN-10His-StayGold
Plasmid#211360PurposeBacterial expression of the bright and photostable StayGold fluorescent protein. Codon optimised for expression in Escherichia coli. Contains a N-terminal 10xHis tag.DepositorInsertStayGold
Tags10xHis-tagExpressionBacterialPromoterT7Available SinceDec. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCx55.5-TrpR
Plasmid#86373Purposefor zebrafish transgenesis, expression of TrpR from Cx55.5 promoter, also expresses mCherryDepositorInsertTrpR
UseZebrafishPromotercx55.5Available SinceAug. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
p201H Cas9
Plasmid#59176PurposeCas9 driven by double 35S, hygromycin resistance for plant selection, I-PpoI site to accept gRNA from pUC gRNA ShuttleDepositorInsertsCas9
hph
UseCRISPRPromoter2x35S and StUbi-3PAvailable SinceAug. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pNP2157
Plasmid#222732PurposepNP1950 - J23119-IS621 bRNA TBL-WT, DBL-WT, HDV; T7-IS621 RecombinaseDepositorInsertIS621 Helper (WT)
ExpressionBacterialAvailable SinceJuly 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a_T7-ARG2
Plasmid#106474PurposeExpresses acoustic reporter genes or gas vesicles (ARG2) in Escherichia coliDepositorInsertARG2
UseSynthetic BiologyExpressionBacterialPromoterT7Available SinceMarch 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSMART-SOS-GFPuv
Plasmid#102283PurposeFluorescent sensor of E. coli SOS responseDepositorInsertcda-GFPuv
ExpressionBacterialPromotercda SOS promoterAvailable SinceApril 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMS82
Plasmid#68110PurposeIntegration vector derived from Streptomyces phage phiBT1. Hygromycin resistance gene, oriT, ColEi origin of replicationDepositorInsertsaac3(IV)
Hyg
oriT
attP
int
UseStreptomyces integrating vectorMutationFragmentAvailable SinceJuly 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pVS2
Plasmid#33144DepositorInsertsLacO (256 copies)
GAL-GFP-kd200-GALpA reporter
ExpressionYeastMutationGFP is mutated such that amino acids 64-73 (FSYGV…Promoterbidirectional GAL1-GAL10 promoterAvailable SinceDec. 20, 2011AvailabilityAcademic Institutions and Nonprofits only -
pBEL1092
Plasmid#195635PurposeExpression/purification of 6xHis-TEV-sfGFPDepositorInsertsfGFP
ExpressionBacterialAvailable SinceMay 2, 2023AvailabilityAcademic Institutions and Nonprofits only