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171,702 results

Showing: 4801 - 4850 of 171702 results

  3. P362-FBP_12

    Plasmid
    #139703
    Purpose
    T-DNA encoding Fungal Bioluminescent Pathway Components, complete pathway
    Depositor
    Daniel Voytas
    Article
    Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1
    Insert
    pSlH4:NnCPH:tSlH4, p35S_Short_TMV 5':AsNpga:tAtug7, pAtuMas:NhH3H:tAtuMas, p35S:NnHisps:t35S, p35S:NnLuz:AtuOcs
    Use
    Luciferase
    Expression
    Plant
    Promoter
    various
    Available Since
    May 29, 2020
    Availability
    Academic Institutions and Nonprofits only
    139703-plasmid-map-sequence-id-296521

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  4. lenti-EF1a-dCas9-VPR-eGFP

    Plasmid
    #241307
    Purpose
    3rd generation lenti vector encoding dCas9 (s. Pyogenes) fused with the VP64-p65-Rta(VPR) and a 2A GFP fluorescence marker
    Depositor
    Robby Bowles
    Insert
    eGFP
    Use
    Lentiviral
    Available Since
    Nov. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    241307-plasmid-map-sequence-id-483039

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  5. pWPXL

    Plasmid
    #12257
    Purpose
    2nd generation lentiviral transfer plasmid
    Depositor
    Didier Trono
    Type
    Empty backbone
    Use
    Cre/Lox and Lentiviral
    Tags
    EGFP
    Expression
    Mammalian
    Available Since
    Aug. 4, 2006
    Availability
    Academic Institutions and Nonprofits only
    12257-plasmid-map-sequence-id-237089

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  8. pET-pA-Hia5

    Plasmid
    #174372
    Purpose
    SEE DEPOSITOR COMMENTS BELOW. Plasmid for IPTG-inducible expression and purification of protein A-Hia5-6xHis (pA-Hia5) for antibody directed DNA (adenine) methylation (as in DiMeLo-seq)
    Depositor
    Aaron Straight
    Article
    Altemose et al Nat Methods. 2022 Jun;19(6):711-723. doi
    Insert
    protein A - hia5
    Tags
    6xHis and Protein A
    Expression
    Bacterial
    Available Since
    Sept. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    174372-plasmid-map-sequence-id-341860

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  12. pSBbi-pur H-2Kb

    Plasmid
    #111623
    Purpose
    Sleeping Beauty transposon vector encoding H-2Kb mouse MHC Class I allele
    Depositor
    Jon Yewdell
    Insert
    H2Kb
    Expression
    Mammalian
    Promoter
    EF1alpha
    Available Since
    June 18, 2018
    Availability
    Academic Institutions and Nonprofits only
    111623-plasmid-map-sequence-id-213220

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  19. pCRISPR-mcBEST

    Plasmid
    #209416
    Purpose
    Plasmid for multiplexed cytosine base editing in streptomycetes
    Depositor
    Tilmann Weber
    Article
    Whitford et al ACS Synth Biol. 2023 Aug 18;12(8):2353-2
    Inserts
    Codon optimized APOBEC1-nCas9-UGI fusion protein
    csy4 from Pseudomonas aeruginosa PAO1
    Use
    CRISPR and Synthetic Biology
    Promoter
    base editing cassette: PtipA ; sgRNAs: PkasO* an…
    Available Since
    Dec. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    209416-plasmid-map-sequence-id-416613

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  25. pLV[Expression]-mCherry:T2A:Hygro-EF1A>Luc2

    Plasmid
    #174665
    Purpose
    Expresses humanized firefly luciferase and mCherry and Hygromycin resistance linked by T2A, each controlled by a strong promoter
    Depositor
    Eli Bar
    Inserts
    LUC2
    mCherry:T2A:Hygro
    Use
    Lentiviral and Luciferase
    Tags
    mCherry and hygromycin dual reporter gene linked …
    Expression
    Mammalian
    Promoter
    Human cytomegalovirus immediate early enhancer/pr…
    Available Since
    Oct. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    174665-plasmid-map-sequence-id-344886

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  31. pOpen-K12polLF (exo-)

    Plasmid
    #165522
    Purpose
    DNA pol fragment used in flourescent labelling for microarray, dA and dT tailing, and ligating DNA adapters to DNA fragments.
    Depositor
    Drew Endy, Jennifer Molloy, FreeGenes Project
    Insert
    DNA Polymerase I, Large (Klenow) Fragment (3'-5' exo-)
    Use
    Synthetic Biology
    Available Since
    Dec. 21, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165522-plasmid-map-sequence-id-350938

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  36. GLP1R-DuET

    Plasmid
    #213253
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    GLP1R (GLP1R Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213253-plasmid-map-sequence-id-430803

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  38. pcDNA4TO-SCN8A-Variant-1-IRES-mScarlet

    Plasmid
    #162280
    Purpose
    Eukaryotic expression of human SCN8A variant 1 isoform. This channel has been modified to be stably maintained in standard bacterial strains.
    Depositor
    Al George
    Article
    DeKeyser et al J Biol Chem. 2021 Jan-Jun;296:100298. do
    Insert
    SCN8A Variant 1, stabilized (SCN8A Human)
    Tags
    IRES-mScarlet
    Expression
    Mammalian
    Mutation
    Modified human HSPA5 intron 4 inserted after bp23…
    Promoter
    CMV
    Available Since
    June 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    162280-plasmid-map-sequence-id-334621

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  39. HA-CHMP4B

    Plasmid
    #139013
    Purpose
    Expresses HA-tagged human CHMP4B in mammalian cells
    Depositor
    Ken-Ichi Takemaru
    Insert
    human CHMP4B
    Tags
    HA
    Expression
    Mammalian
    Available Since
    March 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    139013-plasmid-map-sequence-id-274403

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  44. pAAV-hsyn-GRAB_DA2h (AAV9)

    Viral Prep
    #140554-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pAAV-hsyn-GRAB_DA2h (#140554). In addition to the viral particles, you will also receive purified pAAV-hsyn-GRAB_DA2h plasmid DNA. Synapsin-driven expression of high-affinity GRAB_DA2h dopamine sensor in neurons. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Yulong Li
    Article
    Sun et al Nat Methods. 2020 Nov;17(11):1156-1166.
    Promoter
    Syn
    Available Since
    Aug. 9, 2021
    Availability
    Academic Institutions and Nonprofits only

  45. pAAV-hsyn-GRAB_rDA1m (AAV9)

    Viral Prep
    #140556-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pAAV-hsyn-GRAB_rDA1m (#140556). In addition to the viral particles, you will also receive purified pAAV-hsyn-GRAB_rDA1m plasmid DNA. Synapsin-driven expression of red fluorescent medium affinity dopamine sensor GRAB_rDA1m. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Yulong Li
    Article
    Sun et al Nat Methods. 2020 Nov;17(11):1156-1166.
    Promoter
    Syn
    Available Since
    Aug. 31, 2021
    Availability
    Academic Institutions and Nonprofits only

  46. pLL3.7

    Plasmid
    #11795
    Purpose
    3rd generation lentiviral vector that expresses shRNA under the mouse U6 promoter. A CMV-EGFP reporter cassette is included in the vector to monitor expression.
    Depositor
    Luk Parijs
    Article
    Rubinson et al Nat Genet. 2003 Mar . 33(3):401-6.
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Cre/Lox, Lentiviral, and RNAi
    Expression
    Mammalian
    Promoter
    mouse U6
    Available Since
    July 19, 2006
    Availability
    Academic Institutions and Nonprofits only
    11795-plasmid-map-sequence-id-237575

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  47. pJR100

    Plasmid
    #187240
    Purpose
    Lentiviral sgRNA vector for Perturb-seq with mU6 sgRNA promoter, CR1 constant region with CS1 capture sequence in stem loop, and UCOE EF1alpha driving PURO-BFP marker expression
    Depositor
    Marco Jost, Jonathan Weissman
    Article
    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75
    Insert
    Lentiviral sgRNA vector for Perturb-seq with mU6 promoter
    Use
    Lentiviral
    Available Since
    Oct. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    187240-plasmid-map-sequence-id-374768

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Showing: 4801 - 4850 of 171702 results