We narrowed to 13,451 results for: Mpl;

1. pRN3P-NLS-EGFP-Btgh-3NLS

   Plasmid

   #194469

   Purpose

   Encodes for bacterial O-GlcNAcase BtGH84 fused to enhanced GFP and NLSs for effective nuclear localization, in backbone for T3 In Vitro Transcription.

   Depositor

   Mathieu Boulard

   Article

   Formichetti et al Dev Cell 10 Jan 2024

   Insert

   BtGH84
   

   Use

   For in vitro transcription from t3 promoter after…

   Tags

   SV40 Nuclear Localization Signal, SV40 Nuclear Lo…

   Expression

   Bacterial

   Available Since

   Jan. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. REEP5-EGFP

   Plasmid

   #210363

   Purpose

   Expresses REEP5 fused with EGFP in mammalian cells

   Depositor

   Michele Pagano

   Article

   Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

   Insert

   REEP5 (REEP5 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Available Since

   Dec. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pMD19-Cas9_handle-S.pyogenes_terminator-PBBa_J23117-Cmr

   Plasmid

   #190794

   Purpose

   Template vector to amplify pieces for creating multiplex sgRNA-arrays.

   Depositor

   Paul Hudson

   Article

   Cengic et al ACS Synth Biol. 2022 Aug 15. doi: 10.102

   Insert

   sgRNA-scaffold only
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   BBa_J23117

   Available Since

   Nov. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSLQ2814 pPB: CAG-Puro-WPRE PGK-VPR-tagBFP-SpdCas9

   Plasmid

   #84247

   Purpose

   Expresses direct fusion VPR-Sp dCas9

   Depositor

   Stanley Qi

   Article

   Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

   Inserts

   VPR-tagBFP-Sp dCas9
   Puro
   

   Use

   CRISPR and Synthetic Biology; Piggybac

   Tags

   2xNLS (SV40), HA tag, VPR, and tagBFP

   Expression

   Mammalian

   Promoter

   CAG and PGK

   Available Since

   Nov. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAIP Nup62Fv2GFP

   Plasmid

   #139684

   Purpose

   Nup62 dimerization construct fused to 2 copies of GFP under an SFFV promoter

   Depositor

   Edward Campbell

   Article

   Dharan et al Nat Microbiol. 2020 Jun 1. pii: 10.1038/

   Insert

   Nup62 (NUP62 Human)
   

   Use

   Lentiviral

   Tags

   2 copies of GFP and Dimerization domain FKBP

   Expression

   Mammalian

   Promoter

   SFFV

   Available Since

   Dec. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. NFAST(low)-mApple-mito

   Plasmid

   #214415

   Purpose

   Mitochondria localization of low-affinity NFAST fused to mApple-mito

   Depositor

   Chi-Lun Chang

   Article

   Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

   Insert

   RspA-N
   

   Tags

   mApple-mito

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pRN3P-HA-Suv4-20h2

   Plasmid

   #86691

   Purpose

   For transcription of of Suv4-20h2 mRNA preceded by an HA-tag in 5'

   Depositor

   Maria-Elena Torres-Padilla

   Article

   Eid et al Genes Dev. 2016 Nov 15;30(22):2513-2526.

   Insert

   histone-lysine N-methyltransferase KMT5C (Kmt5c Mouse)
   

   Tags

   HA

   Promoter

   T3

   Available Since

   March 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCDNA3-mitoT

   Plasmid

   #174519

   Purpose

   Engineered intermembrane bridge to link the inner and outer mitochondrial membranes in mammalian cells

   Depositor

   Oleh Khalimonchuk

   Article

   Viana et al iScience. 2021 Jan 29;24(2):102119. doi:

   Insert

   MitoT synthetic tether
   

   Tags

   6xHis and eGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. Lenti-mApple-6xHp

   Plasmid

   #217376

   Purpose

   Lipid droplet localization of 6xHp of Spastin fused to mApple

   Depositor

   Chi-Lun Chang

   Article

   Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

   Insert

   mApple-6x Hp NC
   

   Use

   Lentiviral

   Tags

   mApple

   Promoter

   CMV

   Available Since

   April 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pFastBac1 Flag BRG1

    Plasmid

    #1957

    Depositor

    Robert Kingston

    Article

    Phelan et al Mol Cell 1999 Feb;3(2):247-53.

    Insert

    BRG1 (SMARCA4 Human)
    

    Tags

    flag

    Expression

    Insect

    Available Since

    July 18, 2006

    Availability

    Academic Institutions and Nonprofits only

11. pscALPS- HIV2-ROD vpx

    Plasmid

    #115816

    Purpose

    Encodes codon optimized 3xFLAG-HIV2ROD Vpx (M15390) and puromycin resistance protein

    Depositor

    Jeremy Luban

    Article

    Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

    Insert

    HIV2ROD Vpx (M15390)
    

    Use

    Lentiviral

    Tags

    3x-FLAG

    Promoter

    SFFV

    Available Since

    Jan. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV-SlugABE-NNG-P2A-puro

    Plasmid

    #214354

    Purpose

    Expresses SlugABE-NNG and puromycin resistance genes

    Depositor

    Yongming Wang

    Article

    Qi et al Nat Chem Biol. 2023 Dec 5. doi: 10.1038/

    Insert

    SlugABE-NNG and puromycin resistance genes
    

    Expression

    Mammalian

    Mutation

    Q782R/S888R/L906R/N984S/E1012K/K1016I

    Available Since

    Feb. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLenti c-MYC-DDK-Puro GFP

    Plasmid

    #123299

    Purpose

    Mammalian expression of GFP C-MYC-FLAG

    Depositor

    Giovanni Tonon

    Article

    Gaviraghi et al EMBO J. 2018 Dec 3;37(23). pii: embj.201

    Insert

    GFP
    

    Use

    Lentiviral

    Tags

    MYC-FLAG

    Expression

    Mammalian

    Available Since

    March 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. FAB(ER-LD)

    Plasmid

    #214424

    Purpose

    Detection of ER-lipid droplet contact site

    Depositor

    Chi-Lun Chang

    Article

    Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

    Insert

    FRB-V5
    

    Tags

    CFAST-Halo and RspAN-FRB-V5

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. C3 luc (T1 del)

    Plasmid

    #11358

    Depositor

    Donald McDonnell

    Article

    Fan et al Mol Endocrinol. 1996 Dec . 10(12):1605-1

    Insert

    C3 promoter (C3 Human)
    

    Use

    Luciferase

    Expression

    Mammalian

    Available Since

    July 19, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. mCherry-C1-GOLGA4-GRIP

    Plasmid

    #220068

    Purpose

    overexpreeses the TGN protein GRIP with a mCherry fluorophore

    Depositor

    Gerry Hammond

    Article

    Doyle et al Contact (Thousand Oaks). 2024 Feb 22;7:2

    Insert

    GOLGA4-GRIP (GOLGA4 Human)
    

    Expression

    Mammalian

    Available Since

    May 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pFastBac-hDicer

    Plasmid

    #89144

    Purpose

    Expression of hDicer in Sf9 Cells

    Depositor

    Jennifer Doudna

    Article

    MacRae et al Proc Natl Acad Sci U S A. 2008 Jan 15;10

    Insert

    hDicer (DICER1 Human)
    

    Tags

    His6-tev

    Expression

    Insect

    Available Since

    April 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pT7-EGFP-C1-HsNot8

    Plasmid

    #37367

    Depositor

    Elisa Izaurralde

    Article

    Braun et al Mol Cell. 2011 Oct 7;44(1):120-33.

    Insert

    HsNot8 (CNOT8 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 13, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCMV-SlugCas9-NNG-P2A-puro

    Plasmid

    #214352

    Purpose

    Expresses SlugCas9-NNG and puromycin resistance genes

    Depositor

    Yongming Wang

    Article

    Qi et al Nat Chem Biol. 2023 Dec 5. doi: 10.1038/

    Insert

    SlugCas9-NNG and puromycin resistance genes
    

    Expression

    Mammalian

    Mutation

    Q782R/S888R/L906R/N984S/E1012K/K1016I

    Available Since

    Feb. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3-myc NES-mCE NLS

    Plasmid

    #82466

    Purpose

    Expresses cytoplasmic restricted form of myc tagged mRNA capping enzyme

    Depositor

    Daniel Schoenberg

    Article

    Otsuka et al Mol Cell Biol. 2009 Apr;29(8):2155-67. d

    Insert

    NES mRNA capping enzyme (Rngtt HIV, Mouse)
    

    Tags

    Myc

    Expression

    Mammalian

    Available Since

    Sept. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pET15b SARS-CoV PLpro

    Plasmid

    #61693

    Purpose

    inducible expression of SARS PLpro protease

    Depositor

    Michael Johnson

    Article

    Lee et al ChemMedChem. 2013 Aug;8(8):1361-72. doi:

    Insert

    SARS-CoV PLpro
    

    Tags

    His

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 10, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

22. pLYS5-MCU-Flag

    Plasmid

    #50054

    Purpose

    Expression of human MCU in mammalian cells

    Depositor

    Vamsi Mootha

    Article

    Sancak et al Science. 2013 Nov 14.

    Insert

    MCU-Flag (MCU Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    Jan. 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pAd-Track Flag-SIRT1

    Plasmid

    #8438

    Depositor

    Pere Puigserver

    Article

    Rodgers et al Nature 2005 Mar 3;434(7029):113-8.

    Insert

    SIRT1 (Sirt1 Mouse)
    

    Use

    Adenoviral

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    Dec. 4, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pENTR221-NR1H3

    Plasmid

    #79514

    Purpose

    MultiSite Gateway entry clones, second fragment (attL5, attL2) Human NR1H3 CDS

    Depositor

    Peter Jon Nelson

    Article

    Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

    Insert

    LXR Alpha (NR1H3 Human)
    

    Use

    Multisite gateway entry clone

    Available Since

    May 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCS2TAL3-DD

    Plasmid

    #37275

    Purpose

    TALEN backbone vector for use with the Golden Gate TALEN Kit in place of pTAL1-4. Shorter N- and C-terminal tal protein segments increases mutation induction; Fok I DD (improved version=pCS2TAL3-DDD)

    Depositor

    David Grunwald

    Article

    Dahlem et al PLoS Genet. 2012 Aug;8(8):e1002861. Epub

    Type

    Empty backbone

    Tags

    Flag, Fok I DD, Tal-C, and Tal-N

    Expression

    Bacterial and Mammalian

    Promoter

    CMV

    Available Since

    June 27, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pMXs-IP GFP-Atg14

    Plasmid

    #38264

    Depositor

    Noboru Mizushima

    Article

    Itakura et al Mol Biol Cell. 2008 Dec . 19(12):5360-72

    Insert

    autophagy related genes 14 (ATG14 Human)
    

    Use

    Retroviral

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    LTR

    Available Since

    Aug. 17, 2012

    Availability

    Academic Institutions and Nonprofits only

27. pYO1125

    Plasmid

    #235742

    Purpose

    Protein expression of GST-tagged PX domain (Cys at 2) in bacteria

    Depositor

    Roger Williams

    Article

    Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

    Insert

    p40 PX domain (NCF4 Human)
    

    Tags

    GST-TEV

    Expression

    Bacterial

    Mutation

    aa 2-149 with Cys at 2

    Available Since

    April 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pBMN iRFP670-OPTN(D474N)

    Plasmid

    #119693

    Purpose

    For mammalian expression of iRFP-tagged UBD mutant OPTN

    Depositor

    Michael Lazarou

    Article

    Padman et al Nat Commun. 2019 Jan 24;10(1):408. doi:

    Insert

    OPTN (OPTN Human)
    

    Use

    Retroviral

    Tags

    iRFP670

    Expression

    Mammalian

    Mutation

    D474N

    Available Since

    Jan. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pspCas9-Klenow-ST2-com-vector

    Plasmid

    #176235

    Purpose

    Vector plasmid for expressing spCas9-Klenow fusion protein and sgRNA. There are two copies of HBB 3’ UTR in the 3’UTR of spCas9-Klenow to enhance expression.

    Depositor

    Baisong Lu

    Article

    Yoo et al Nucleic Acids Res. 2022 Mar 22. pii: 655

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    March 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pMSCV-puro-NKX2-8

    Plasmid

    #75080

    Purpose

    retroviral vector holding human NKX2-8 cDNA

    Depositor

    David Mu

    Article

    Kendall et al Proc Natl Acad Sci U S A. 2007 Oct 16;10

    Insert

    NKX2-8 (NKX2-8 Human)
    

    Use

    Retroviral

    Tags

    none

    Expression

    Mammalian

    Mutation

    wild-type, full-length, without native 3' UTR

    Available Since

    May 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. NSP3-FR-2Strep

    Plasmid

    #210315

    Purpose

    Expresses SARS-CoV-2 NSP3 in mammalian cells

    Depositor

    Michele Pagano

    Article

    Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

    Insert

    NSP3 (ORF1ab SARS-CoV-2)
    

    Tags

    2Strep

    Expression

    Mammalian

    Available Since

    Dec. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pFast-Flag-hHus1

    Plasmid

    #222470

    Purpose

    Expresses hHus1 in insect cells

    Depositor

    Aziz Sancar

    Article

    Lindsey-Boltz et al Proc Natl Acad Sci U S A. 2001 Sep 25;98

    Insert

    hHus1 (HUS1 Human)
    

    Tags

    Flag epitope

    Expression

    Insect

    Available Since

    Dec. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. ER-ABKAR

    Plasmid

    #61508

    Purpose

    Expression of AMPK biosensor targeted to the endoplasmic reticulum

    Depositor

    Takanari Inoue, Jin Zhang

    Article

    Miyamoto et al Cell Rep. 2015 Apr 28;11(4):657-70. doi:

    Insert

    AMPK Biosensor
    

    Tags

    ER targeting sequence from CYP450 2C1 (see commen…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 17, 2015

    Availability

    Academic Institutions and Nonprofits only

34. pTS110 pHAGE2 CMVtetO2 MCS-22xGS-Alfa-P2A-TagBFP

    Plasmid

    #199363

    Purpose

    Lentiviral transfer plasmid for doxycycline inducible expression of a C-terminally ALFA-P2A-BFP-tagged protein in mammalian cells.

    Depositor

    Rebecca Voorhees

    Article

    Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    ALFA-P2A-TagBFP

    Expression

    Mammalian

    Mutation

    WT

    Available Since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. px458_2A_GFP_sgRNA_RACK1

    Plasmid

    #127123

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

    Insert

    gRNA RACK1 (RACK1 Human)
    

    Use

    CRISPR

    Available Since

    July 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. 3xFLAG-SENP6

    Plasmid

    #42887

    Depositor

    Edward Yeh

    Article

    Dou et al Mol Cell. 2010 Aug 13. 39(3):333-45.

    Insert

    SENP6 (SENP6 Human)
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Please see depositor comments

    Available Since

    April 23, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pLX302 OAS2-V5 puro

    Plasmid

    #158642

    Purpose

    Lentiviral expression vector for constitutive expression of V5-tagged OAS2.

    Depositor

    Kevin Janes

    Article

    Lopacinski et al Cell Syst. 2021 Apr 21;12(4):304-323.e13

    Insert

    OAS2 (OAS2 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Available Since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pJRH623

    Plasmid

    #171633

    Purpose

    2nd generation lentiviral transfer plasmid encoding a U6 promoter expressing a non-targeting sgRNA and an EF1-a promoter expressing mNeonGreen

    Depositor

    Jennifer Doudna

    Article

    Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

    Inserts

    spyCas9 sgRNA-non-targeting
    mNeon
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    EF1-a and U6

    Available Since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pcDNA3 Flag TSC2 (S939A)

    Plasmid

    #14132

    Depositor

    Brendan Manning

    Article

    Manning et al Mol Cell. 2002 Jul . 10(1):151-62.

    Insert

    TSC2 S939A (TSC2 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    S939A.

    Available Since

    March 2, 2007

    Availability

    Academic Institutions and Nonprofits only

40. pBITE-EGFRvIII-F271-OKT3

    Plasmid

    #190682

    Purpose

    Encodes an EGFRvIII-CD3 BITE protein (F271 clone)

    Depositor

    Scott McComb

    Article

    Shepherd et al PLoS One. 2023 Jun 22;18(6):e0273884. do

    Insert

    Anti-EGFRvIII-scFv-F271-modularized-OKT3-HisTag
    

    Use

    Lentiviral

    Tags

    mNeonGreen

    Expression

    Mammalian

    Promoter

    EFS

    Available Since

    Feb. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pWzl_CRKL-Y207F+W275L

    Plasmid

    #196022

    Purpose

    Expresses CRKL Y207F + W275L

    Depositor

    William Hahn

    Article

    Cheung et al Cancer Discov. 2011 Dec;1(7):608-25. doi

    Insert

    CRKL
    

    Use

    Retroviral

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pWzl_CRKL-W275L

    Plasmid

    #196019

    Purpose

    Expresses CRKL W275L

    Depositor

    William Hahn

    Article

    Cheung et al Cancer Discov. 2011 Dec;1(7):608-25. doi

    Insert

    CRKL
    

    Use

    Retroviral

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pCMV-Neo-Bam E2F6

    Plasmid

    #37965

    Depositor

    Jacqueline Lees

    Article

    Trimarchi et al Proc Natl Acad Sci U S A. 2001 Feb 13;98

    Insert

    E2F6 (E2F6 Human)
    

    Expression

    Mammalian

    Available Since

    July 18, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pcDNA/Myc DeltaN TCF4

    Plasmid

    #16513

    Depositor

    Bert Vogelstein

    Article

    Korinek et al Science. 1997 Mar 21. 275(5307):1784-7.

    Insert

    TCF4 (TCF7L2 Human)
    

    Expression

    Mammalian

    Mutation

    Delta N: lacks aa 1-32

    Available Since

    May 16, 2008

    Availability

    Academic Institutions and Nonprofits only

45. pUCmEos3.2:cat

    Plasmid

    #78100

    Purpose

    Plasmid template that encodes the photoconvertible fluorescent protein mEos3.2 next to chloramphenicol resistance gene (cat). This template was used to copy out mEos3.2-cat for genetic tagging.

    Depositor

    Peng Chen

    Article

    Chen et al Nat Commun. 2015 Jul 6;6:7445. doi: 10.1

    Insert

    mEos3.2-cat
    

    Expression

    Bacterial

    Available Since

    June 7, 2016

    Availability

    Academic Institutions and Nonprofits only

46. pMT-Hrp48-ADARcd-E488Q-V5

    Plasmid

    #139685

    Purpose

    Expresses the hyperactive form of the catalytic domain of the ADAR protein fused to HRP-48 for use in "TRIBE" assays

    Depositor

    Michael Rosbash

    Article

    Xu et al RNA. 2018 Feb;24(2):173-182. doi: 10.126

    Insert

    Hrp48, ADAR (Adar Fly)
    

    Tags

    V5

    Expression

    Bacterial and Insect

    Available Since

    March 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. STAgR_Neo

    Plasmid

    #102992

    Purpose

    Can be used as backbone for a STAgR reaction

    Depositor

    Stefan Stricker

    Article

    Breunig et al PLoS One. 2018 Apr 27;13(4):e0196015. do

    Type

    Empty backbone

    Use

    CRISPR; Pcr template for stagr vectors

    Promoter

    U6

    Available Since

    Feb. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. PM-ABKAR

    Plasmid

    #61506

    Purpose

    Expression of AMPK biosensor targeted to the plasma membrane via Lyn kinase targeting sequence

    Depositor

    Takanari Inoue, Jin Zhang

    Article

    Miyamoto et al Cell Rep. 2015 Apr 28;11(4):657-70. doi:

    Insert

    AMPK Biosensor
    

    Tags

    Lyn kinase sequence (see comments)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 17, 2015

    Availability

    Academic Institutions and Nonprofits only

49. pTS98 pHAGE2 CMVtetO2 TagBFP-P2A-ALFA-22xGS-MCS

    Plasmid

    #199351

    Purpose

    Lentiviral transfer plasmid for doxycycline inducible expression of a N-terminally BFP-P2A-ALFA-tagged protein in mammalian cells.

    Depositor

    Rebecca Voorhees

    Article

    Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    TagBFP-P2A-ALFA

    Expression

    Mammalian

    Mutation

    WT

    Available Since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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50. pEGFP-C1-Nup35-mut

    Plasmid

    #87341

    Purpose

    To express human Nup35 in mammalian cells. It contains synonymous mutations that are not targeted by shRNA

    Depositor

    Lei Lu

    Article

    Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

    Insert

    NUP35 (NUP35 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    shRNA resistant changes

    Promoter

    CMV

    Available Since

    May 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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