159,557 results

1. pEvolvR-enCas9-PolI3M-TBD

   Plasmid

   #113077

   Purpose

   Expresses enCas9 fused to PolI3M-TBD in E. coli. contains a BsmbI-flanked GFP cassette for sgRNA cloning.

   Depositor

   David Schaffer, John Dueber

   Article

   Halperin et al Nature. 2018 Aug 1. pii: 10.1038/s41586-

   Insert

   enCas9-PolI3M-TBD
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAG-HIF1 alpha

   Plasmid

   #21101

   Depositor

   Connie Cepko

   Article

   Chen et al Science. 2009 Jan 9. 323(5911):256-9.

   Insert

   HYPOXIA INDUCIBLE FACTOR 1ALPHA (HIF1A Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 5, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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3. TFORF1207

   Plasmid

   #144089

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   CASZ1 (CASZ1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   June 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pUltra-chPheRS

   Plasmid

   #182257

   Purpose

   Genetic Incorporation of DOPA

   Depositor

   Han Xiao

   Article

   Chen et al J Mol Biol. 2021 Dec 20:167412. doi: 10.

   Inserts

   chPheRS2 for DOPA
   chPheT for DOPA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. NpY-mNeon

   Plasmid

   #186618

   Purpose

   Encodes Neuropeptide Y fused to mNeonGreen

   Depositor

   Gia Voeltz

   Article

   Zamponi et al Dev Cell. 2022 Jun 6;57(11):1369-1382.e6

   Insert

   Neuropeptide Y (NPY Human)
   

   Use

   Tags

   mNeonGreen

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. mNeonGreen-mTurquoise2

   Plasmid

   #98886

   Purpose

   Produces a fusion between mTurquoise2 and mNeonGreen. It can be used as a positive control for FRET

   Depositor

   Dorus Gadella

   Article

   Mastop et al Sci Rep. 2017 Sep 20;7(1):11999. doi: 10

   Insert

   mNeonGreen-mTurquoise2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pANAP

   Plasmid

   #48696

   Purpose

   Allows co-translational incorporation of the fluorescent ncAA Anap directly into the target protein; expresses amber suppressor tRNA and the synthetase AnapRS

   Depositor

   Peter Schultz

   Article

   Chatterjee et al J Am Chem Soc. 2013 Aug 28;135(34):12540

   Inserts

   8 copies of the amber suppressor tRNACUA-Leu
   AnapRS
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   mutant E.coli leucyl synthetase specific for Anap…

   Promoter

   CMV and human H1 promoter

   Available since

   Nov. 25, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA5-TOM20_Halo_T2A_EGFP

   Plasmid

   #135443

   Purpose

   Mamalian cell expression of mictochondrial HaloTag7 and cytosolic EGFP

   Depositor

   Kai Johnsson

   Article

   Frei et al Nat Commun. 2019 Oct 8;10(1):4580. doi:

   Insert

   TOM20-HaloTag7 and EGFP
   

   Use

   Tags

   TOM20

   Expression

   Mammalian

   Mutation

   Promoter

   CMV/TetO

   Available since

   Jan. 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. Flag-Gsdmd-NT 4A

   Plasmid

   #80952

   Purpose

   Expresses Gsdmd-NT 4A mutant in mammalian cells

   Depositor

   Judy Lieberman

   Article

   Liu et al Nature. 2016 Jul 6;535(7610):153-8. doi:

   Insert

   Gsdmd-NT 4A (Gsdmd Mouse)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. mCherry-pCAG-donor_opt(R2Tg)-GFP(intron)

    Plasmid

    #223249

    Purpose

    Mammalian expression of the optimized R2Tg RNA donor

    Depositor

    Wei Li

    Article

    Chen et al Cell. 2024 Jul 1:S0092-8674(24)00694-9.

    Insert

    R2Tg donor_opt
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. EC67907

    Plasmid

    #211794

    Purpose

    Over expresses Cas9 and GR-GRF-GIF. Must receive guide cassettes

    Depositor

    Cristobal Uauy, Wendy Harwood

    Article

    Lawrenson et al Research Square, 12 March 2024, PREPRINT

    Insert

    HptII, Cas9, GR-GRF-GIF
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Jan. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. VP12

    Plasmid

    #72247

    Purpose

    Human expression plasmid for SpCas9-HF1 variant: CMV-T7-humanSpCas9-HF1(N497A, R661A, Q695A, Q926A)-NLS-3xFLAG

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1

    Insert

    mammalian codon-optimized Streptococcus pyogenes Cas9 HF1(N497A/R661A/Q695A/Q926A)-NLS-3xFlag
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Mammalian

    Mutation

    N497A, R661A, Q695A and Q926A

    Promoter

    CMV

    Available since

    Jan. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. EC70364

    Plasmid

    #209515

    Purpose

    Cloning in complete guide expression cassettes, with Cas9

    Depositor

    Cristobal Uauy, Wendy Harwood

    Article

    Lawrenson et al Research Square, 12 March 2024, PREPRINT

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. Ddo_porcupine-1

    Plasmid

    #107631

    Purpose

    In situ hybridization and RNAi studies in planarians

    Depositor

    Alejandro Sánchez

    Article

    Accorsi et al The American Biology Teacher 79(3):208-2

    Insert

    porcupine-1
    

    Use

    RNAi; In situ hybridization

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-EFS-SpCas9

    Plasmid

    #104588

    Purpose

    AAV vector expressing Myc-tagged SpCas9 under the EFS promoter

    Depositor

    Ryohei Yasuda

    Article

    Nishiyama et al Neuron. 2017 Oct 18. pii: S0896-6273(17)

    Insert

    Streptococcus pyogenes Cas9
    

    Use

    AAV and CRISPR

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available since

    Dec. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pZac2.1 gfaABC1D-cyto-GCaMP8s

    Plasmid

    #186052

    Purpose

    AAV-mediated expression of GCaMP8s under the control of GfaABC1D promoter

    Depositor

    Loren Looger

    Insert

    jGCaMP8s
    

    Use

    AAV

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    GfaABC1D

    Available since

    Aug. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBFC0984

    Plasmid

    #231992

    Purpose

    CRISPRi-ART plasmid encoding aTc-inducible dRfxCas13d and constitutive crRNA with 2xBsaI spacer cloning site

    Depositor

    Brady Cress

    Article

    Adler et al Nat Microbiol. 2025 Mar;10(3):694-709. d

    Inserts

    dRfxCas13d
    crRNA with 2xBsaI spacer
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23119 and pTet

    Available since

    April 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCasPA

    Plasmid

    #113347

    Purpose

    Bacterial expression of Cas9 nuclease and λ-Red system in Pseudomonas aeruginosa

    Depositor

    Quanjiang Ji

    Article

    Chen et al iScience. 2018 Aug 31;6:222-231. doi: 10

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. alpha7nACHR_Nacho_GCAMP7s

    Plasmid

    #182940

    Purpose

    alpha7 nACHR expression in mammalian cell lines with calcium reporter

    Depositor

    Robert Brenner

    Article

    Belanger-Coast et al Eur J Pharmacol. 2022 Jun 15;925:175000.

    Insert

    NACH7 (CHRNA7 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    May 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. NG-ABEmax

    Plasmid

    #124163

    Purpose

    A-to-G base editor

    Depositor

    David Liu

    Article

    Huang et al Nat Biotechnol. 2019 May 20. pii: 10.103

    Insert

    TadA-TadA(evo)-Cas9-NG
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. Nsp7-mCherry

    Plasmid

    #165134

    Purpose

    mammalian expression and localization

    Depositor

    Bruno Antonny

    Article

    Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

    Insert

    Nsp7 (ORF1ab codon optimized SARS-COV-2)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pRluc-LC3G120A

    Plasmid

    #105003

    Purpose

    Mammalian expression of Rluc-LC3G120A. Can be used for measuring autophagic flux.

    Depositor

    Marja Jããttelã

    Article

    Farkas et al Methods Enzymol. 2017;588:1-13. doi: 10.

    Insert

    LC3B (Map1lc3b Rat)
    

    Use

    Luciferase

    Tags

    Rluc with C124A substitution

    Expression

    Mammalian

    Mutation

    Glycine 120 mutated to alanine, prevents lipidati…

    Promoter

    Available since

    Jan. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. MiniCoopR mitfa:EGFP

    Plasmid

    #118850

    Purpose

    Expresses EGFP and zebrafish mitfa specifically in zebrafish melanocytes

    Depositor

    Leonard Zon

    Article

    Ablain et al Science. 2018 Nov 1. pii: science.aau650

    Insert

    mitfa: EGFP (mitfa Zebrafish, Synthetic)
    

    Use

    Zebrafish expression, tol6

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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24. 6xHis-SNAP-PI(3,4,5)P3 Probe

    Plasmid

    #211514

    Purpose

    For expression of recombinant biosensor for detection of Phosphatidylinositol-3,4,5-Trisphosphate (PI(3,4,5)P3) from bacteria

    Depositor

    David Murray, Hannes Maib

    Article

    Maib et al J Cell Biol. 2024 Jun 3;223(6):e20231009

    Insert

    BTK1-PH
    

    Use

    Tags

    6xhis-SNAP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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25. MAC-FURIN

    Plasmid

    #158386

    Purpose

    MAC-tagged gene expression

    Depositor

    Markku Varjosalo

    Article

    Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

    Insert

    furin, paired basic amino acid cleaving enzyme (FURIN Human)
    

    Use

    Tags

    MAC-tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 25, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

26. pT7II-2N4Rtau

    Plasmid

    #177653

    Purpose

    Recombinant protein synthesis of human Tau

    Depositor

    Jeff Kuret

    Article

    Ayoub et al PLoS Genet. 2023 Mar 27;19(3):e1010681.

    Insert

    Tau (2N4R) (MAPT Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pRluc-LC3wt

    Plasmid

    #105002

    Purpose

    Mammalian expression of Rluc-LC3wt. Can be used for measuring autophagic flux.

    Depositor

    Marja Jããttelã

    Article

    Farkas et al Methods Enzymol. 2017;588:1-13. doi: 10.

    Insert

    LC3B (Map1lc3b Rat)
    

    Use

    Luciferase

    Tags

    Rluc with C124A substitution

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pHTN-HaloTag_BCL11A:1-773

    Plasmid

    #211014

    Purpose

    Mammalian expression of full-length human BCL11A. N-terminal HaloTag.

    Depositor

    Cheryl Arrowsmith

    Article

    Ackloo et al bioRxiv 2024.03.03.583197

    Insert

    BCL11A:M1-F773 (BCL11A Human)
    

    Use

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    isoform 2

    Promoter

    CMV

    Available since

    Feb. 21, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

29. pUbC-FLAG-24xSuntagV4-oxEBFP-AID-baUTR1-24xMS2V5-Wpre

    Plasmid

    #84561

    Purpose

    pSINAPS; single molecule translation reporter containing a FLAG tag, Suntag, oxBFP, AID, beta-actin 3'UTR, and MS2V5 stem-loops

    Depositor

    Robert Singer

    Article

    Wu et al Science. 2016 Jun 17;352(6292):1430-5. d

    Insert

    FLAG tag, Suntag, oxBFP, Auxin-inducible degron, beta-actin 3'UTR, MS2V5 stem-loops
    

    Use

    Lentiviral

    Tags

    Flag, Suntag, oxBFP, AID (auxin-inducible degron)

    Expression

    Mutation

    Promoter

    UbC

    Available since

    Dec. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pETMCSI-T4P_Y320A

    Plasmid

    #122522

    Purpose

    Expression in BL21(DE3) PlysS of mutant T4 DNA polymerase Y320A for easy cloning and mutagenesis

    Depositor

    Gottfried Otting

    Article

    Qi et al PLoS One. 2019 Jan 23;14(1):e0211065. do

    Insert

    gp43 - DNA polymerase (43 Escherichia virus T4)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    tyrosine 320 changed to alanine

    Promoter

    T7 promoter

    Available since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge