159,557 results

1. pEvolvR-enCas9-PolI3M-TBD

   Plasmid

   #113077

   Purpose

   Expresses enCas9 fused to PolI3M-TBD in E. coli. contains a BsmbI-flanked GFP cassette for sgRNA cloning.

   Depositor

   David Schaffer, John Dueber

   Article

   Halperin et al Nature. 2018 Aug 1. pii: 10.1038/s41586-

   Insert

   enCas9-PolI3M-TBD
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. 6xHis-SNAP-PI(3,4)P2 Probe

   Plasmid

   #211511

   Purpose

   For expression of recombinant biosensor for detection of Phosphatidylinositol-3,4-Bisphosphate (PI(3,4)P2) from bacteria

   Depositor

   Hannes Maib, David Murray

   Article

   Maib et al J Cell Biol. 2024 Jun 3;223(6):e20231009

   Insert

   TAPP1 cPHx3
   

   Use

   Tags

   6xhis-SNAP

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pX330_DDX4-cterm

   Plasmid

   #222520

   Purpose

   Cas9/sgRNA plasmid for targeting DDX4

   Depositor

   George Church

   Article

   Pierson Smela et al bioRxiv 2024.05.31.596483

   Insert

   Cas9, DDX4 sgRNA (DDX4 Synthetic, Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. NG-ABEmax

   Plasmid

   #124163

   Purpose

   A-to-G base editor

   Depositor

   David Liu

   Article

   Huang et al Nat Biotechnol. 2019 May 20. pii: 10.103

   Insert

   TadA-TadA(evo)-Cas9-NG
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUbC-FLAG-24xSuntagV4-oxEBFP-AID-baUTR1-24xMS2V5-Wpre

   Plasmid

   #84561

   Purpose

   pSINAPS; single molecule translation reporter containing a FLAG tag, Suntag, oxBFP, AID, beta-actin 3'UTR, and MS2V5 stem-loops

   Depositor

   Robert Singer

   Article

   Wu et al Science. 2016 Jun 17;352(6292):1430-5. d

   Insert

   FLAG tag, Suntag, oxBFP, Auxin-inducible degron, beta-actin 3'UTR, MS2V5 stem-loops
   

   Use

   Lentiviral

   Tags

   Flag, Suntag, oxBFP, AID (auxin-inducible degron)

   Expression

   Mutation

   Promoter

   UbC

   Available since

   Dec. 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. mNeonGreen-mTurquoise2

   Plasmid

   #98886

   Purpose

   Produces a fusion between mTurquoise2 and mNeonGreen. It can be used as a positive control for FRET

   Depositor

   Dorus Gadella

   Article

   Mastop et al Sci Rep. 2017 Sep 20;7(1):11999. doi: 10

   Insert

   mNeonGreen-mTurquoise2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAG-tdTomato

   Plasmid

   #83029

   Purpose

   expresses tdTomato fluorescent protein under the CAG promoter

   Depositor

   Angelique Bordey

   Article

   Pathania et al PLoS One. 2012;7(5):e38174. doi: 10.1371

   Insert

   tdTomato
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Sept. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSAM_Ec

   Plasmid

   #102939

   Purpose

   Transposon mutagenesis and Tn-seq

   Depositor

   Matthew Mulvey

   Article

   Wiles et al PLoS Genet. 2013;9(8):e1003716. doi: 10.

   Insert

   mariner transposon flanked by MmeI modified inverted repeats and the himar1C9 transposase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1-ERBB4-V5/HIS

   Plasmid

   #201105

   Purpose

   expression of human ERBB4 receptor tyrosine kinase in mammalian cells

   Depositor

   Pavel Krejčí

   Article

   Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

   Insert

   human ERBB4 receptor tyrosine kinase, full length, wildtype (ERBB4 Human)
   

   Use

   Tags

   V5/His

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. PB_iMYOD1_P2A_GFP_Puro

    Plasmid

    #168804

    Purpose

    PiggyBac transposon construct with doxycycline inducible MYOD1 + GFP expression

    Depositor

    Prashant Mali

    Article

    Dailamy et al Stem Cell Reports. 2021 Sep 8. pii: S221

    Insert

    MYOD1 (MYOD1 Synthetic, Human)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available since

    Aug. 31, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAG-mtagBFP

    Plasmid

    #89685

    Purpose

    pCAG promoter driving mtagBFP

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

    Insert

    mtagBFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. N-Cadherin-EGFP

    Plasmid

    #18870

    Depositor

    Valeri Vasioukhin

    Article

    Nechiporuk et al Dev Cell. 2007 Sep . 13(3):338-50.

    Insert

    N-Cadherin (Cdh2 Mouse)
    

    Use

    Tags

    EGFP, Kanamycin, and N-Cadherin

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 13, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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13. Tet-on circRNA Expression Vector

    Plasmid

    #92351

    Purpose

    The vector can ef_ciently produce a circRNA (circmCherry) from a split EGFP gene after Dox treatment.

    Depositor

    Ling-Ling Chen

    Article

    Zhang et al Cell Rep. 2016 Apr 19;15(3):611-24. doi:

    Insert

    mCherry and split EGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pTet

    Available since

    Sept. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGL4.23-5xE-box

    Plasmid

    #206841

    Purpose

    The plasmid contains a 312 bp DNA fragment that contained 5 canonical E-boxes (GCCACGTGCA) spaced by 50 nucleotides and cloned into pGL4.23[luc2/minP] (XhoI/HindIII).

    Depositor

    Tassula Proikas-Cezanne

    Article

    Sporbeck et al Commun Biol. 2023 Aug 24;6(1):872. doi:

    Insert

    5x E-box ((GCCACGTGCA)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pmRFP_a_tubulin_IRES_puro2b

    Plasmid

    #21041

    Depositor

    Daniel Gerlich

    Article

    Steigemann et al Cell. 2009 Feb 6. 136(3):473-84.

    Insert

    alpha Tubulin (TUBA1B Human)
    

    Use

    Tags

    pmRFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 9, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLX313-TP53-WT

    Plasmid

    #118014

    Purpose

    Constitutive expression of wild-type human Tumor Protein p53 (TP53)

    Depositor

    David Root, William Hahn

    Article

    Giacomelli et al Nat Genet. 2018 Oct;50(10):1381-1387. do

    Insert

    TP53 (TP53 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    Oct. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. Tet-inducible Luciferase reporter

    Plasmid

    #64127

    Purpose

    Photoactivatable transcription system. Luciferase reporter under the control of a tet-inducible promoter.

    Depositor

    Moritoshi Sato

    Article

    Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

    Insert

    luciferase
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TetOx13-CMV

    Available since

    April 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pK18sB

    Plasmid

    #177838

    Purpose

    Plasmid for gene replacement using kanamycin/sucrose selection/counterselection. Derived from pK18mobsacB but reduced in size and lacking oriT required for conjugation.

    Depositor

    Gregg Beckham, Christopher Johnson

    Article

    Jayakody et al Energy Environ. Sci., 2018,11, 1625-1638

    Type

    Empty backbone

    Use

    Bacterial gene replacement

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBMN(CMV-copGFP-Puro-SMAR)

    Plasmid

    #80391

    Purpose

    expression copGFP and Puromycine, with an S/MAR element for episomal maintaince

    Depositor

    Magnus Essand

    Article

    Jin et al EMBO Mol Med. 2016 May 6. pii: e20150586

    Insert

    copGFP-T2A-Puromycine
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pTK194

    Plasmid

    #59563

    Purpose

    TC-83 I-SceI T7 5'UTR (A3G) P1234 (nsP2 Q739L) SGP XbaI attB1 Kozak mKate opal attB2 AscI (truncated E1) 3'UTR Poly A I-SceI

    Depositor

    Ron Weiss

    Article

    Wroblewska et al Nat Biotechnol. 2015 Aug 3. doi: 10.1038

    Insert

    SGP XbaI attB1 Kozak mKate opal attB2 AscI
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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21. pCDNA3 NQO1-Flag

    Plasmid

    #61729

    Purpose

    Expresses human NQO1 Flag tagged at C-terminus

    Depositor

    Yosef Shaul

    Article

    Tsvetkov et al J Biol Chem. 2011 Mar 18;286(11):8839-45

    Insert

    NAD(P)H dehydrogenase, quinone 1 (NQO1) (NQO1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    cmv

    Available since

    April 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pcDNA-dCas9

    Plasmid

    #47106

    Purpose

    Expresses inactivated S. pyogenes dCas9 (D10A, H840A) in mammalian cells

    Depositor

    Charles Gersbach

    Article

    Perez-Pinera et al Nat Methods. 2013 Jul 25. doi: 10.1038/n

    Insert

    dCas9
    

    Use

    CRISPR

    Tags

    Flag and SV40 NLS

    Expression

    Mammalian

    Mutation

    D10A, H840A (catalytically inactive)

    Promoter

    CMV

    Available since

    Nov. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pAAV-CAG-iGluSnFR4s-NGR-WPRE

    Plasmid

    #234451

    Purpose

    AAV-mediated expression of glutamate sensor with improved sensitivity and slow deactivation kinetics (4s = slow); NGR vector matches or outperforms PDGFR in all conditions tested.

    Depositor

    GENIE Project

    Article

    Aggarwal et al bioRxiv 2025.03.20.643984

    Insert

    iGluSnFR4s-NGR
    

    Use

    AAV

    Tags

    IgK chain and Myc epi tag-NGR GPI anchor

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pLD-puro-MTS-EGFP

    Plasmid

    #190270

    Purpose

    Lentiviral conditional expression of mitochondrial-targeted eGFP in mammalian cells

    Depositor

    Christian Münch

    Article

    Michaelis et al Bio Protoc. 2022 Dec 20;12(24):e4578. do

    Insert

    MTS-EGFP
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Tight TRE promoter

    Available since

    Sept. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. ptf-Galectin3

    Plasmid

    #64149

    Purpose

    Expression in mammalian cells

    Depositor

    Tamotsu Yoshimori

    Article

    Maejima et al EMBO J. 2013 Aug 28;32(17):2336-47. doi:

    Insert

    Galectin3 (LGALS3 Human)
    

    Use

    Tags

    EGFP and mRFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pTNS1

    Plasmid

    #64967

    Purpose

    Tn7 transposase expression

    Depositor

    Herbert Schweizer

    Article

    Choi et al Nat Methods. 2005 Jun;2(6):443-8.

    Insert

    tnsABCD
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 23, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

27. pcDNA GFP10-ZIP-GFP11

    Plasmid

    #182243

    Purpose

    Tripartite split-GFP mammalian. Express leucine zipper with GFP10 and GFP11 (self-assembing with GFP1-9)

    Depositor

    Stéphanie Cabantous

    Article

    Cabantous et al Sci Rep. 2013 Oct 4;3:2854. doi: 10.1038

    Insert

    GFP10-ZIP-11
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pT3TS-RfxCas13d-NLS-HA

    Plasmid

    #141321

    Purpose

    Plasmid to carry out IVT of RfxCas13d-NLS (human codon-optimized)

    Depositor

    Ariel Bazzini, Miguel Angel Moreno-Mateos

    Article

    Kushawah et al Dev Cell. 2020 Sep 28;54(6):805-817.e7.

    Insert

    Rfx-Cas13d (NLS-RfxCas13d-NLS)
    

    Use

    CRISPR

    Tags

    HA and NLS

    Expression

    Mutation

    Promoter

    Available since

    Aug. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pcDNA3.1-CHRNA7-YFP

    Plasmid

    #62630

    Purpose

    mammalian expression of human Alpha7 (CHRNA7) with YFP fused into M3-M4 loop

    Depositor

    Henry Lester

    Article

    Wang et al J Biol Chem. 2014 Sep 19;289(38):26451-6

    Insert

    hAlpha7 (CHRNA7 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    YFP inserted in M3-M4 loop

    Promoter

    CMV

    Available since

    March 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. gRNA_AAVS1-T2

    Plasmid

    #41818

    Purpose

    Expresses a guide RNA (gRNA) to target human AAVS1 (T2 target sequence) for genome engineering

    Depositor

    George Church

    Article

    Mali et al Science. 2013 Feb 15;339(6121):823-6. do

    Insert

    gRNA_AAVS1-T2
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge