168,035 results

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pET-6xHis-GFP(0)

Plasmid

#199166

Purpose

used to express a neutral GFP variant in E. coli

Depositor

Allie Obermeyer

Article

Yeong et al ACS Cent Sci. 2020 Dec 23;6(12):2301-231

Insert

GFP(0)

Tags

MGHHHHHHGGA

Mutation

E16R, I138R, D207R, N222K, L246R

Promoter

T7

Available Since

May 10, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-N-Flag-NLRP3 1-90

Plasmid

#75137

Purpose

Expresses N-terminal Flag-tagged amino acids 1-90 of NLRP3 (the pyrin domain) in mammalian cells

Depositor

Bruce Beutler

Article

Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:

Insert

NLR family, pyrin domain containing 3 (Nlrp3 Mouse)

Tags

Flag

Expression

Mammalian

Mutation

fragment encoding amino acids 1-90

Promoter

CMV

Available Since

May 20, 2016

Availability

Academic Institutions and Nonprofits only

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pCS2-UTX-F

Plasmid

#17438

Purpose

Expression of UTX in mammalian cells

Depositor

Kai Ge

Article

Hong et al Proc Natl Acad Sci U S A. 2007 Nov 20. 1

Insert

UTX (KDM6A Human)

Tags

6xMyc and Flag

Expression

Mammalian

Available Since

Feb. 19, 2008

Availability

Academic Institutions and Nonprofits only

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pMXs-hSOX2-17

Plasmid

#193350

Purpose

Retroviral vector expressing human SOX2-17 chimeric transcription factor (super-SOX)

Depositor

Hans Schöler

Article

MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

Insert

SOX2-17 (SOX2 Human)

Use

Retroviral

Expression

Mammalian

Mutation

SOX2-17 is engineered highly cooperative chimeric…

Promoter

5'LTR

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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PGK-H2BeGFP

Plasmid

#21210

Depositor

Mark Mercola

Article

Kita-Matsuo et al PLoS ONE. 2009 . 4(4):e5046.

Insert

Phosphoglycerate kinase promoter (PGK1 Human)

Use

Lentiviral

Tags

H2B-eGFP

Available Since

July 1, 2009

Availability

Academic Institutions and Nonprofits only

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pMXs-Oct3/4

Plasmid

#13366

Purpose

Retroviral expression of mouse Oct3/4

Depositor

Shinya Yamanaka

Article

Takahashi et al Cell. 2006 Aug 25. 126(4):663-76.

Insert

POU domain, class 5, transcription factor 1 (Pou5f1 Mouse)

Use

Retroviral

Expression

Mammalian

Mutation

None

Available Since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only

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pAAV.hSynapsin.SF-iGluSnFR.A184S

Plasmid

#106174

Purpose

Tight affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

Has Service

AAV1 and AAV9

Insert

SF-iGluSnFR.A184S

Use

AAV

Mutation

GltI: A184S

Promoter

hSynapsin

Available Since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR.v2.TetON_Cas13d_U6_DR

Plasmid

#196727

Purpose

Sortable and selectable, Tet-inducible RfxCas13d with guide cassette (one vector system). Inducible knock-down.

Depositor

Bernhard Schmierer

Insert

RfxCas13d-T2A-miRFP670nano

Use

CRISPR and Lentiviral

Tags

Nucleoplasmin NLS and SV40 NLS

Promoter

TRE

Available Since

April 12, 2023

Availability

Academic Institutions and Nonprofits only

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anxA2-GFP

Plasmid

#107196

Purpose

Expresses human annexin A2-GFP fusion protein for live cell imaging

Depositor

Volker Gerke, Ursula Rescher

Article

Rescher et al J Cell Sci. 2000 Nov;113 ( Pt 22):3931-8

Insert

annexin A2 (ANXA2 Human)

Tags

Green Fluorescent Protein

Expression

Mammalian

Mutation

Ala residue 65 in human sequence replaced by Glu …

Promoter

CMV

Available Since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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MCP-mCherry

Plasmid

#207668

Purpose

To express fluorescently labeled MS2 coat binding protein (MCP)

Depositor

Christine Mayr

Article

Horste et al Mol Cell. 2023 Dec 21;83(24):4509-4523.e

Insert

MCP-mCherry

Expression

Mammalian

Available Since

Sept. 26, 2023

Availability

Academic Institutions and Nonprofits only

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CMV-mito-LAR-GECO1.2

Plasmid

#61245

Purpose

Expresses LAR-GECO1.2 in the mitochondria in mammalian cells

Depositor

Robert Campbell

Article

Wu et al Biochem J. 2014 Nov 15;464(1):13-22. doi

Insert

LAR-GECO1.2

Tags

a duplex of the mitochondrial targeting signal of…

Expression

Mammalian

Mutation

Substitutions relative to R-GECO1.2: N45I/A47R/E1…

Promoter

CMV

Available Since

March 27, 2015

Availability

Academic Institutions and Nonprofits only

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pOmnibac_ZZ_linker_K963_GFP_SNAP

Plasmid

#159727

Purpose

Expresses kinesin-1 (amino acids 1-963) in Sf9 cells with a C-terminal GFP and SNAP tag

Depositor

Ahmet Yildiz

Article

Ferro et al Science. 2022 Jan 21;375(6578):326-331.

Insert

KIF5B (full length, 1-963 amino acids), K963 (KIF5B Human)

Tags

GFP and SNAP tags and ZZ affinity tag

Expression

Insect

Available Since

Jan. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-CKIIa-stGtACR1-FusionRed

Plasmid

#105679

Purpose

Expresses a soma-targeted GtACR1 in mammalian neurons under the CaMKIIa promoter

Depositor

Ofer Yizhar

Article

Mahn et al Nat Commun. 2018 Oct 8;9(1):4125. doi: 1

Insert

stGtACR1

Use

AAV

Tags

FusionRed and Kv2.1

Expression

Mammalian

Promoter

CaMKIIa

Available Since

Feb. 23, 2018

Availability

Academic Institutions and Nonprofits only

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pM201

Plasmid

#173179

Purpose

dCas9-24xGCN4

Depositor

Hajin Kim

Article

Chaudhary et al Genome Res. 2020 Sep;30(9):1306-1316. do

Insert

dCas9-24xGCN4

Use

CRISPR and Lentiviral

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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hCas9-VPR

Plasmid

#68497

Purpose

nuclease competent SP-Cas9 fused to VPR

Depositor

George Church

Article

Kiani et al Nat Methods. 2015 Sep 7. doi: 10.1038/nm

Insert

SP-Cas9-VPR

Tags

VPR

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 8, 2015

Availability

Academic Institutions and Nonprofits only

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pAC-BETA

Plasmid

#53272

Purpose

Contains crtE, crtB, crtI, and crtY carotenoid pathway genes of Erwinia herbicola (Pantoea agglomerans) Eho10 and thereby produces beta-carotene in Escherichia coli

Depositor

Francis X Cunningham Jr

Article

Cunningham FX et al Plant Cell. 1996 Sep;8(9):1613-26.

Insert

crtE, crtY, crtI, crtB

Use

Low copy number bacterial cloning vector

Promoter

endogenous promoters

Available Since

Jan. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pTET2-RlucCP-3MS2

Plasmid

#198354

Purpose

Part of Renilla reporter system, encodes Renilla luciferase with MS2 stem loops in the 3'UTR

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

Renilla Luciferase

Use

Luciferase

Expression

Mammalian

Promoter

pTET2

Available Since

April 20, 2023

Availability

Academic Institutions and Nonprofits only

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pAT9651-BEAR-GFP

Plasmid

#162989

Purpose

BEAR target plasmid with split EGFP and disrupted 5' splice site

Depositor

Ervin Welker

Article

Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

Insert

EGFP split with an intron between amino acids 95-96

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pCMMP-MCS-IRES-eGFP-P2A-iCas9

Plasmid

#178533

Purpose

For genome editing: EGFP-inducible Caspase9 knock-in cassette which allows ablation of target cells

Depositor

Ryoji Yao

Article

Okamoto et al STAR Protoc. 2021 Sep 16;2(4):100780. do

Insert

iCaspase9 (CASP9 Human)

Expression

Mammalian

Mutation

FKBP12 F36 to V Truncated Caspase9 (135-416; lack…

Available Since

Jan. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pSBbi-BN

Plasmid

#60518

Purpose

SB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: BFP and neomycin resistance gene

Depositor

Eric Kowarz

Article

Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b

Type

Empty backbone

Use

Transposon

Expression

Mammalian

Promoter

EF1a/RPBSA

Available Since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pGEX-5X-1-LARP7

Plasmid

#113545

Purpose

Produces an N-terminal GST-tagged full length LARP7 protein.

Depositor

Blerta Xhemalce

Article

Shelton et al Cell Rep. 2018 Feb 6;22(6):1374-1383. do

Insert

LARP7 (LARP7 Human)

Tags

GST

Expression

Bacterial

Available Since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

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pCDH-3_Flag-PABPC1

Plasmid

#232944

Purpose

Expresses PABPC1 protein

Depositor

Jianjun Chen

Article

Chen et al Cell. 2024 Dec 12:S0092-8674(24)01324-2.

Insert

PABPC1 (PABPC1 Human)

Use

Lentiviral

Available Since

March 19, 2025

Availability

Academic Institutions and Nonprofits only

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pgRNA-CKB

Plasmid

#73501

Purpose

gRNA expression vector (with mKate2)

Depositor

Bruce Conklin

Article

Mandegar et al Cell Stem Cell. 2016 Apr 7;18(4):541-53.

Insert

mKate2-T2A-Bsd

Use

CRISPR and Lentiviral

Tags

NLS

Expression

Mammalian

Promoter

CAG

Available Since

Dec. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pLentiRNACRISPR_012-TetO-NLS-inactive-hfCas13d-NLS-WPRE-EFS-rtTA3-2A-Blast

Plasmid

#228558

Purpose

Expresses inactive hfCas13d in mammalian cells for binding of target RNAs in combination with compatible crRNAs. Localized to the nucleus

Depositor

Neville Sanjana

Article

Hart et al Nat Biotechnol. 2025 Feb 11. doi: 10.103

Insert

dhfCas13d (N2V8)

Use

Lentiviral

Tags

NLS-HA

Mutation

R239A/H244A/R858A/H863A

Available Since

Aug. 19, 2025

Availability

Academic Institutions and Nonprofits only

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DsRed2-pBAD

Plasmid

#54608

Purpose

Localization: Protein Expression Vector , Excitation: 558, Emission: 583

Depositor

Michael Davidson

Type

Empty backbone

Tags

6xHis and DsRed2

Expression

Bacterial

Available Since

July 25, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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cyto-Ruby3-iATPSnFR1.0

Plasmid

#102551

Purpose

expresses cytoplasmic ATP sensor in mammalian cells with red reference protein

Depositor

Baljit Khakh

Article

Lobas et al Nat Commun. 2019 Feb 12;10(1):711. doi:

Insert

Ruby3-iATPSnFR1.0

Tags

Ruby3

Expression

Mammalian

Available Since

Jan. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pLP_mod_mNeonGreen11_P2A_puroR_CRE-GFP

Plasmid

#229227

Purpose

Modular BxB1 landing pad vector containing C-term mNeonGreen11 tag, puroR marker, and a cAMP GFP reporter.

Depositor

Willow Coyote-Maestas

Article

Howard et al Cell. 2025 Feb 6;188(3):671-687.e20. doi

Type

Empty backbone

Tags

mNeonGreen11

Expression

Mammalian

Available Since

Dec. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-H2B-GFP

Plasmid

#116869

Purpose

Nuclear localized GFP

Depositor

Loren Looger

Article

Economo et al Nature. 2018 Nov;563(7729):79-84. doi: 1

Has Service

AAV Retrograde

Insert

GFP

Use

AAV

Available Since

Nov. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pJL1-YPet

Plasmid

#102633

Purpose

In vitro expression of YPet from the T7 promoter

Depositor

Michael Jewett

Article

Stark et al Sci Adv. 2018 Aug 1;4(8):eaat5107. doi:

Insert

YPet

Expression

Bacterial

Promoter

T7

Available Since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

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CIRTS-1: ORF5-TBP6.7-Pin domain-NLS

Plasmid

#132543

Purpose

Expresses CIRTS-1 (ORF5-TBP6.7-Pin domain-NLS) in mammalian cells

Depositor

Bryan Dickinson

Article

Rauch et al Cell. 2019 Jun 27;178(1):122-134.e12. do

Insert

CIRTS-1: ORF5-TBP6.7-Pin domain-NLS

Tags

FLAG tag

Expression

Mammalian

Promoter

cmv d0

Available Since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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