161,600 results

1. CAG FUCCI

   Plasmid

   #175266

   Purpose

   Bicistronic FUCCI construct driven by a CAG promoter. Contains I-SceI sites for transgenesis

   Depositor

   James Monaghan

   Article

   Duerr et al Development. 2022 Mar 15;149(6). pii: 27

   Insert

   FUCCI
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. 3XFlagNICD4

   Plasmid

   #20186

   Depositor

   Raphael Kopan

   Article

   Ong et al J Biol Chem. 2006 Feb 24. 281(8):5106-19

   Insert

   Notch 4 Intracellular Domain (Notch4 Mouse)
   

   Use

   Tags

   3X Flag

   Expression

   Mammalian

   Mutation

   Contains murine Notch 4 Intracellular Domain (Val…

   Promoter

   CMV

   Available since

   Dec. 13, 2012

   Availability

   Academic Institutions and Nonprofits only

3. pLenti CMV/TO Neo DEST (685-3)

   Plasmid

   #17292

   Purpose

   3rd gen lentiviral Gateway destination vector, expression, CMV/TO promoter, Neo

   Depositor

   Paul Kaufman, Eric Campeau

   Article

   Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

   Type

   Empty backbone

   Use

   Lentiviral; Destination vector

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 12, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBBR1.cas9-NT

   Plasmid

   #197871

   Purpose

   Constitutive expression of Cas9 endonuclease, trancRNA and crRNA with a randomly generated spacer sequence (non-targeting)

   Depositor

   Baojun Wang

   Article

   Huan et al ACS Synth Biol. 2023 Mar 17;12(3):709-72

   Inserts

   trancCRNA
   Cas9
   crRNA (non-chromosomal (E. coli/S. flexneri)-targeting spacer sequence)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGH125_dCas9-Blast

   Plasmid

   #85417

   Purpose

   lentiviral expression vector for dCas9 with Blasticidin selectable marker

   Depositor

   Michael Bassik

   Article

   Hess et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

   Insert

   dCas9 and Blasticidin resistance
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBAD-mGreenLantern

   Plasmid

   #161913

   Purpose

   mGreenLantern fluorescent protein: bacterial expression plasmid

   Depositor

   Gregory Petsko

   Article

   Campbell et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

   Insert

   mGreenLantern
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   araB

   Available since

   Nov. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1/cyto-iAspSnFR

   Plasmid

   #201394

   Purpose

   CMV overexpression of cyto-iAspSnFR in mammalian cell lines

   Depositor

   Kai Johnsson

   Article

   Hellweg et al Cell Chem Biol. 2024 May 20:S2451-9456(2

   Insert

   cyto-iAspSnFR
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   gltI5-253: Y42F, S72A, T92D, G111T, K192R, Y211W,…

   Promoter

   CMV

   Available since

   May 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3-TLR8-YFP

   Plasmid

   #13024

   Depositor

   Doug Golenbock

   Insert

   Toll-Like Receptor 8 (TLR8 Human)
   

   Use

   Tags

   YFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 3, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-nEF-Coff/Fon-ChRmine-oScarlet

   Plasmid

   #137160

   Purpose

   Intersectional viral expression of ChRmine-p2a-oScarlet in cells expressing Flp AND NOT Cre

   Depositor

   INTRSECT 2.0 Project, Karl Deisseroth

   Article

   Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

   Has Service

   AAV8

   Insert

   Coff/Fon-ChRmine-p2a-oScarlet
   

   Use

   AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

   Tags

   Expression

   Mutation

   oScarlet E95D

   Promoter

   nEF

   Available since

   June 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pYTK-DN4

    Plasmid

    #180285

    Purpose

    KanR multi-gene yeast expression vector (GFP-dropout)

    Depositor

    Andreas Milias-Argeitis

    Article

    Novarina et al STAR Protoc. 2022 Jun 10;3(2):101358. do

    Insert

    ConLS' - GFP - ConRE' - KanR - CEN6/ARS4
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    June 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBbE8a-RFP

    Plasmid

    #35268

    Depositor

    Jay Keasling

    Article

    Lee et al J Biol Eng. 2011 Sep 20;5:12.

    Insert

    RFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    BAD

    Available since

    Feb. 16, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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12. CL20_mEGFP_PML_RARA

    Plasmid

    #205897

    Purpose

    Express mEGFP-tagged fusion protein, PML_RARA from patient-derived sequence

    Depositor

    Richard Kriwacki

    Article

    Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

    Insert

    PML_RARA (PML, RARA Human)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pdRBFC-NS1-YC

    Plasmid

    #74724

    Purpose

    Express C-terminal YC fused influenza A virus partial NS1 protein (27-236 nt) for dsRNA labeling

    Depositor

    Article

    Cheng et al Virology. 2015 Nov;485:439-51. doi: 10.1

    Insert

    Influenza A virus NS1 protein, partial
    

    Use

    Double-stranded rna labeling

    Tags

    FLAG and YFP C-terminal half domain

    Expression

    Plant

    Mutation

    Promoter

    CaMV 35S

    Available since

    May 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-Ef1a-DIO-ChRmine-mScarlet-WPRE

    Plasmid

    #130998

    Purpose

    Double floxed ChRmine-mScarlet under the control of Ef1a promoter

    Depositor

    Karl Deisseroth

    Article

    Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

    Has Service

    AAV1 and AAV5

    Insert

    ChRmine
    

    Use

    AAV

    Tags

    mScarlet

    Expression

    Mutation

    Promoter

    Ef1a

    Available since

    Oct. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pFUSEss-CHIg-mG1_M18

    Plasmid

    #82357

    Purpose

    Expression plasmid coding for heavy chain of mouse IgG1 antibody specific to antigen B of the ABO blood group system, clone M18.

    Depositor

    Joanna Bereta

    Article

    Klaus et al Sci Rep. 2016 Aug 3;6:30938. doi: 10.103

    Insert

    mouse immunoglobulin heavy chain IgG1 isotype (Ighg1 Mouse)
    

    Use

    Tags

    none

    Expression

    Mammalian

    Mutation

    none

    Promoter

    hEF1-HTLV prom

    Available since

    Sept. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. GST-BHMT-I/G

    Plasmid

    #104442

    Purpose

    Autophagy cargo assay

    Depositor

    Carol Mercer

    Article

    Mercer et al Autophagy. 2008 Feb;4(2):185-94. Epub 20

    Insert

    BHMT (BHMT Human)
    

    Use

    Tags

    GFP-myc GFP is from Aequorea victoria and GST

    Expression

    Mammalian

    Mutation

    N43A in GST (in a potential KFERQ-like sequence).…

    Promoter

    CMV

    Available since

    Dec. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRK5-HA-Ubiquitin-K6

    Plasmid

    #22900

    Depositor

    Sandra Weller

    Article

    Livingston et al PLoS Pathog. 2009 Oct . 5(10):e1000619.

    Insert

    Ubiquitin C (UBC Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    K6 only, all other lysines mutated to arginines

    Promoter

    Available since

    Jan. 22, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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18. miRFP670nano-pcDNA

    Plasmid

    #127443

    Purpose

    Monomeric near-infrared fluorescent protein miRFP670nano in mammalian plasmid

    Depositor

    Vladislav Verkhusha

    Article

    Oliinyk et al Nat Commun. 2019 Jan 17;10(1):279. doi:

    Insert

    miRFP670nano
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-FLEX-EGFPL10a

    Plasmid

    #98747

    Purpose

    Cre-dependently expresses the fusion protein EGFPL10a

    Depositor

    Alexander Nectow, Eric Schmidt, Nathaniel Heintz

    Article

    Nectow et al Cell Rep. 2017 Apr 18;19(3):655-667. doi

    Has Service

    AAV Retrograde and AAV5

    Insert

    EGFPL10a
    

    Use

    AAV and Cre/Lox

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Aug. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. mEmerald-CLIP170-N-18

    Plasmid

    #54044

    Purpose

    Localization: Microtubule Binding , Excitation: 487, Emission: 509

    Depositor

    Michael Davidson

    Insert

    CLIP170 (CLIP1 Human)
    

    Use

    Tags

    mEmerald

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 2, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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21. pHR-FUSN-mCh-Cry2olig

    Plasmid

    #101224

    Purpose

    FUS(1-214) fused to mCh-Cry2olig

    Depositor

    Clifford Brangwynne

    Article

    Shin et al Cell. 2017 Jan 12;168(1-2):159-171.e14.

    Insert

    FUS(aa1-214) (FUS Human)
    

    Use

    Lentiviral

    Tags

    mCherry-Cry2olig

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    Feb. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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22. pCS2-LAMP1-mRFP

    Plasmid

    #169004

    Purpose

    Express rat LAMP1-mRFP in zebrafish

    Depositor

    Noboru Mizushima

    Article

    Morishita et al Cell Rep. 2020 Dec 8;33(10):108477. doi:

    Insert

    rat LAMP1-mRFP
    

    Use

    Unspecified

    Tags

    mRFP

    Expression

    Mutation

    Promoter

    Available since

    April 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. mEmerald-Alpha-V-Integrin-25

    Plasmid

    #53985

    Purpose

    Localization: Focal Adhesions, Excitation: 487, Emission: 509

    Depositor

    Michael Davidson

    Insert

    Alpha-V Integrin (ITGAV Human)
    

    Use

    Tags

    mEmerald

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 16, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

24. pmiRA1-MYB

    Plasmid

    #141120

    Purpose

    Lentiviral overexpression of MYB in mammalian cells.

    Depositor

    Jianjun Chen

    Article

    Weng et al Cell Stem Cell. 2018 Feb 1;22(2):191-205

    Insert

    MYB (MYB Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pAAV-Ef1a-DIO-ChRmine-mScarlet-Kv2.1-WPRE

    Plasmid

    #130999

    Purpose

    Double floxed soma-targeted ChRmine-mScarlet under the control of Ef1a promoter

    Depositor

    Karl Deisseroth

    Article

    Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

    Insert

    ChRmine
    

    Use

    AAV

    Tags

    mScarlet-Kv2.1

    Expression

    Mutation

    Promoter

    Ef1a

    Available since

    Sept. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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26. ITPKA-mNeonGreen

    Plasmid

    #98883

    Purpose

    In vivo visualization of actin cytoskeleton (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Chertkova et al bioRxiv 160374

    Insert

    ITPKA
    

    Use

    Tags

    mNeonGreen

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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27. pSFFV_sfCherry3V(1-10)

    Plasmid

    #117483

    Purpose

    Lentiviral vector for constitutive expression of sfCherry3V(1-10)

    Depositor

    Bo Huang

    Article

    Feng et al Commun Biol. 2019 Sep 17;2:344. doi: 10.

    Insert

    sfCherry3V(1-10)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    6 substitutions (K45R, G52D, L83W, T106A, K182R, …

    Promoter

    SFFV

    Available since

    July 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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28. RBM10_pLX307

    Plasmid

    #98371

    Purpose

    Lentiviral expression of RBM10

    Depositor

    Sefi Rosenbluh, William Hahn

    Article

    Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

    Insert

    RBM10 (RBM10 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Isoform 2

    Promoter

    E1Fa

    Available since

    Aug. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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29. Ai162 (TIT2L-GC6s-ICL-tTA2) targeting vector

    Plasmid

    #114433

    Purpose

    Target a Cre-dependent GCaMP6s cassette and a tTA2 cassette into the mouse TIGRE locus

    Depositor

    Hongkui Zeng

    Article

    Daigle et al Cell. 2018 Jul 12;174(2):465-480.e22. do

    Insert

    GCaMP6s, tTA2
    

    Use

    Cre/Lox and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    TRE2, CAG

    Available since

    Jan. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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30. qTAG-C-mStayGold-Blast

    Plasmid

    #227254

    Purpose

    Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mStayGold-2A-Blast cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge