29,744 results
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pETM11-SUMO3- sfGFP-KSR1-CA3 (N-KSR)
Plasmid#217767PurposeFluorescent reporter for glucosyl-ceramide (putative, mammalian expression)DepositorInsertKinase suppressor of ras 1 CA3 domain (KSR1 Human)
UseProtein purification (6xhis tag, tev site, sumo3 …TagssfGFPExpressionBacterialMutationCA3 domain aa 317-400PromoterT7 promoterAvailable sinceJune 10, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pETM11-SUMO3-KSR1-CA3-sfGFP (C-KSR)
Plasmid#217766PurposeFluorescent reporter for ceramide (for bacterial expression and purification)DepositorInsertKinase suppressor of ras 1 CA3 domain (KSR1 Human)
UseProtein purification (6xhis tag, tev site, sumo3 …TagssfGFPExpressionBacterialMutationCA3 domain aa 317-400 plus vector-based linker LE…PromoterT7 promoterAvailable sinceJune 10, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSC101-GFPmut2-mScarlet-I TAG
Plasmid#208188Purposenonsense mistranslation, TAG stopDepositorInsertsGFPmut2
mScarlet-I
UseReporterTagsExpressionBacterialMutationInsertion 6TAG*(stop) 7AGT(Ser)PromoterPtet+dnaK P1Available sinceMarch 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSC101-GFPmut2-mScarlet-I TGA
Plasmid#208189Purposenonsense mistranslation, TGA stopDepositorInsertsGFPmut2
mScarlet-I
UseReporterTagsExpressionBacterialMutationInsertion 6TGA*(stop) 7AGT(Ser)PromoterPtet+dnaK P1Available sinceMarch 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSC101-GFPmut2-mScarlet-I TGATC
Plasmid#208190Purposenonsense mistranslation, TGA stop variant 2DepositorInsertsGFPmut2
mScarlet-I
UseReporterTagsExpressionBacterialMutationInsertion 6TGA*(stop) 7TCT(Ser)PromoterPtet+dnaK P1Available sinceMarch 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSC101-GFPmut2-mScarlet-I T7
Plasmid#208191Purposeframeshift mistranslation, T7DepositorInsertsGFPmut2
mScarlet-I
UseReporterTagsExpressionBacterialMutationFrameshift insertion 6TTTTTTTPromoterPtet+dnaK P1Available sinceMarch 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSC101-GFPmut2-mScarlet-I A5
Plasmid#208192Purposeframeshift mistranslation, A5DepositorInsertsGFPmut2
mScarlet-I
UseReporterTagsExpressionBacterialMutationFrameshift insertion 6AAAAAPromoterPtet+dnaK P1Available sinceMarch 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSC101-GFPmut2-mScarlet-I A4
Plasmid#208193Purposeframeshift mistranslation, A4DepositorInsertsGFPmut2
mScarlet-I
UseReporterTagsExpressionBacterialMutationFrameshift insertion 6AAAAPromoterPtet+dnaK P1Available sinceMarch 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSC101-GFPmut2-mScarlet-I A6T
Plasmid#208194Purposeframeshift mistranslation, A6TDepositorInsertsGFPmut2
mScarlet-I
UseReporterTagsExpressionBacterialMutationFrameshift insertion 6AAAAAATPromoterPtet+dnaK P1Available sinceMarch 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSC101-GFPmut2-mScarlet-I C3T
Plasmid#208195Purposeframeshift mistranslation, C3TDepositorInsertsGFPmut2
mScarlet-I
UseReporterTagsExpressionBacterialMutationFrameshift insertion 6CCCTPromoterPtet+dnaK P1Available sinceMarch 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSC101-GFPmut2(ΔmScarlet)
Plasmid#208185PurposeGFP positive, mScarlet-I negative controlDepositorInsertGFPmut2
UseReporterTagsExpressionBacterialMutationPromoterPtet+dnaK P1Available sinceMarch 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSC101-GFPmut2-mScarlet-I 6Trp7Ser
Plasmid#208186Purposemistranslation GFP and mScarlet-I positive control with Trp insertionDepositorInsertsGFPmut2
mScarlet-I
UseReporterTagsExpressionBacterialMutationInsertion 6Trp7SerPromoterPtet+dnaK P1Available sinceMarch 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
PET-B-eSpCas9-NLS-6xHis
Plasmid#207383PurposeExpression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cellsDepositorInsertB-eSpCas9
UseCRISPRTags6xHisExpressionBacterialMutationK848A, K1003A, R1060A, amino acids 1005-1013 repl…PromoterT7Available sinceOct. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJM251 (miniCTX1-araC-ParaBAD)
Plasmid#110567PurposeIntegration vector for Pseudomonas aeruginosa with araC-ParaBAD inducible promoterDepositorInsertaraC-ParaBAD
UseTagsExpressionBacterialMutationPromoteraraC-ParaBADAvailable sinceMay 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
SARS-CoV-2_N_FL
Plasmid#157867Purposeexpress MBP tagged SARS-CoV-2 Nucleocapsid proteinDepositorInsertN (N SARS-CoV-2)
UseTagsMBPExpressionBacterialMutationPromoterAvailable sinceNov. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAB078d8
Plasmid#79206PurposeDirected Evolution of Protein-Protein InteractionsDepositorInsertPlacZ-opt (OR1+2) luxAB; Ppro1 434cI-SH2ABL1
UseTagsExpressionBacterialMutationPromoterPlacZ-opt (OR1+2); Ppro1Available sinceDec. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
EcMscL
Plasmid#92418PurposeE. coli Mechanosensitive Channel of Large ConductanceDepositorInsertMscL (mscL E. coli)
UseTags6x Histidine Tag and Thrombin Cleavage Site betwe…ExpressionBacterialMutationPromoterT7 PromoterAvailable sinceJuly 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAraGFP
Plasmid#39548DepositorInserteGFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
pBbB5k-GFP
Plasmid#35354DepositorInsertGFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterlacUV5Available sinceFeb. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
pET16b-Trx CC32/35SS
Plasmid#36946DepositorInsertThioredoxin 1 (TXN Human)
UseTagsHis tagExpressionBacterialMutationboth active site cysteines (32,35) mutated to ser…PromoterT7Available sinceMay 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
IL13 promoter luciferase
Plasmid#11784DepositorInsertIL-13 promoter (IL13 Human)
UseLuciferaseTagsluciferaseExpressionBacterialMutationPromoterAvailable sinceMay 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
pEN-dCas9-CDA1-UGI
Plasmid#196248PurposePlasmid containing promoter of RecA from Xanthomonas oryzae driving expression of dead Cas9, cytosine deaminase CDA1, and glycosylase inhibitorDepositorInsertattL4-RecApro-dCas9-CDA1-UGI-attR1
UseTagsExpressionBacterialMutationPromoterRecA promoterAvailable sinceFeb. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGE144 (MutaEco; aTC-induced)
Plasmid#189824PurposeExpresses MutaEco for generating mutations in the E. coli genome (aTC-induced)DepositorInsertEcoRNAPα-PmCDA1
UseTagsExpressionBacterialMutationPromotertetR/tetA promoterAvailable sinceSept. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
371-GFP
Plasmid#97030PurposeHis6-GFP-tev-yORFDepositorTypeEmpty backboneUseTagsGFP fusion, His6 tag, and TEV cleavage siteExpressionBacterialMutationPromoterT7Available sinceJuly 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
PGEX3X-GST-PTEN
Plasmid#20743DepositorInsertPTEN (PTEN Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceMarch 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
pET26b_AT118-L-V5-His
Plasmid#222848PurposeE. coli periplasmid expression plasmid encoding Nb. AT118-L, a humanized nanobody antagonist of the human Angiotensin II type I receptor (AT1R) with reduced polyreactivityDepositorInsertAT118-L
UseTagsV5-HisExpressionBacterialMutationPromoterAvailable sinceSept. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR_full-site
Plasmid#186277PurposeReporter plasmid for full-site selection assayDepositorInsertThiomicrospira leader repeat and modified chloramphenicol selection cassette (removed RBS and start codon))
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBA787
Plasmid#186242Purposesoc-C HR Donor Vector (T4 phage editing)DepositorInsertBBR1 backbone + T4 phage homology
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-20b-M-Ntx-S1
Plasmid#58716PurposeExpresses in Escherichia coli Notexin with a N-terminal extra methionine and with the amino acid substitution N1SDepositorInsertNotexin
UseTagsMethionineExpressionBacterialMutationChanged Asparagine 1 to SerinePromoterT7Available sinceAug. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
His-DERA
Plasmid#203225PurposeFor protein expression. E. coli 2-deoxy-D-ribose-5-phosphate aldolase. Converts dR5P to G3P and acetaldehyde. Pet28a plasmid, KAN resistant, N-terminal TEV-cleavable 6xHis tag.DepositorInsertdeoxyribose aldolase
UseTagsN-terminal TEV cleavable 6xHis tagExpressionBacterialMutationPromoterT7Available sinceJuly 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCS2 PCNA GFP
Plasmid#105937Purposein vitro transcription, cell cycle nuclear markerDepositorInsertPCNA (PCNA Human)
UseTagsGFPExpressionBacterialMutationPromoterAvailable sinceMarch 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pETduet+sfGFP-TetR-p300HAT
Plasmid#157795PurposeExpression of sfGFP-TetR-p300HAT fusion proteinDepositorInsertsfGFP-TetR-p300 (EP300 Human)
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceAug. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLT 651
Plasmid#59998PurposeExpresses a hybrid dsRNA corresponding to the Caenorhabditis elegans klp-16/him-8 genes in bacteria; ingestion of the bacteria by C elegans produces an RNAi response & leads to more male progenyDepositorUseTagsExpressionBacterialMutationpartial genomicPromoterAvailable sinceApril 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTXB1 EGFP
Plasmid#129442Purposeexpression of intein/ gfpDepositorInsertintein/ gfp
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFPV25.1_rpsM_mCerulean3
Plasmid#124904PurposerpsM promoter (S. typhimurium) driving mCerulean3 (codon-optimized for S. typhimurium)DepositorInsertrpsM promoter and mCerulean3
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPROK AdoHcyase
Plasmid#14683DepositorInsertadenosyl-homocysteine hydrolase (AHCY Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 1, 2007AvailabilityAcademic Institutions and Nonprofits only -
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pET_RFP-SZ2
Plasmid#124933PurposeExpression of RFP-SZ2DepositorInsertRFP-SYNZIP2
UseTagsHis and mCherryExpressionBacterialMutationSZ2 domainPromoterAvailable sinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T2-Rheb
Plasmid#15889DepositorInsertRheb (RHEB Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceOct. 26, 2007AvailabilityAcademic Institutions and Nonprofits only -
pHis2-Arf1 (17-181)-Q71L
Plasmid#184808PurposeBacterial expression of Arf1 (aa17-181, Q71L mutation)DepositorInsertArf1 (ARF1 Human)
UseTagsHis6ExpressionBacterialMutationaa 17-181 only, Q71LPromoterT7Available sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
(SUMO)5
Plasmid#126949PurposeBacterial expression plasmid for (SUMO)5 client to assess SUMO-SIM scaffolds.DepositorInsert5 repeats of human SUMO3 separated by (GGS)4 (SUMO3 Human)
UseTagsExpressionBacterialMutationPromoterunknownAvailable sinceNov. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBbB8k-csg-amylase
Plasmid#166859PurposeArabinose-inducible csgBACEFG operon for curli fiber synthesis in which curli subunit CsgA has been fused to α-amylase via a flexible linker. Derived from https://www.addgene.org/35363.DepositorInsertcsgBACEFG, with α-amylase fused to CsgA via flexible linker
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceApril 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
AdML-M3
Plasmid#11244DepositorInsertAdML-M3
UseTags3 MS2 hairpinsExpressionBacterialMutationPromoterAvailable sinceApril 20, 2006AvailabilityAcademic Institutions and Nonprofits only -
Fusion of Zika NS2B-NS3 proteins
Plasmid#204815PurposeBacterial expression of a fusion of Zika NS2B-NS3 proteinsDepositorInsertZika NS2B-NS3 fusion (orf1ab Zika)
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 25, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pKRR5
Plasmid#149468PurposeBroad-Host range expression vector, RK2 and pUC origin, RhaR-RhaS activator, PrhaBAD promoter, mRFP, Gentamicin Resistance Marker.DepositorInsertRhaS-RhaR-mRFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPrhaBADAvailable sinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTU1-A-pdh_RiboJ_mCherry_Bba_B0015
Plasmid#107581PurposeB. megaterium DSM319 pdh promoter, mCherry (Bacillus codon optimised) for Bacillus cell-free transcription-translation. Bacillus shuttle vector backbone, colE1/AmpR (E. coli), RebB/TetA (Bacillus)DepositorInsertmCherry
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterpdh promoter Bacillus megaterium DSM319Available sinceApril 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
His-Tev-WWP2
Plasmid#186807PurposeCodon-optimized bacterial expression plasmid. Expresses ubiquitin ligating enzyme His-Tev-WWP2.DepositorInsertHis-Tev-WWP2 (WWP2 Human)
UseTagsHis6-TevExpressionBacterialMutationPromoterAvailable sinceMarch 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT7C-DNAJA1
Plasmid#177655PurposeRecombinant protein synthesis of human DNAJA1DepositorInsertDNAJA1 (DNAJA1 Human)
UseTags6xHis-ThrombinExpressionBacterialMutationPromoterT7Available sinceMarch 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28-a(+)-6xHis-LSSmCherry1(-13)
Plasmid#219415PurposeFluorescent protein to reconstruct the mixture that mimics the charge profile of E. coli lysateDepositorInsertLSSmCherry1(-13)
UseTagsMGHHHHHGGExpressionBacterialMutationK137E, K199EPromoterT7Available sinceOct. 16, 2024AvailabilityAcademic Institutions and Nonprofits only
