Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

Applied Filters

Bacterial Expression Clear filter

Applied Filters

Bacterial Expression Clear filter

32,069 results

Showing: 4801 - 4840 of 32069 results

  8. pM1s3TsR

    Plasmid
    #137922
    Purpose
    E. coli Nissle pMUT1-derived plasmid with tetracycline resistance and constitutive RFP expression
    Depositor
    Neel Joshi
    Article
    Kan et al ACS Synth Biol. 2020 Dec 10. doi: 10.102
    Insert
    mRFP1
    Use
    Synthetic Biology; For use with e. coli nissle in…
    Expression
    Bacterial
    Promoter
    J23101
    Available Since
    Feb. 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    137922-plasmid-map-sequence-id-323851

    Enlarge

  11. pETM33_Nsp10

    Plasmid
    #156479
    Purpose
    Bacterial expression of Sars-CoV2 Nsp10 protein with His-tag and GST-tag
    Depositor
    Ylva Ivarsson
    Article
    Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:
    Insert
    Nsp10 (ORF1ab Synthetic, Severe acute respiratory syndrome coronavirus 2)
    Tags
    His-GST
    Expression
    Bacterial
    Mutation
    Gene insert is codon optimized for Ecoli.
    Promoter
    T7/LacO
    Available Since
    Aug. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    156479-plasmid-map-sequence-id-307731

    Enlarge

  14. SPLICSFAST-LDh-LY-P2A

    Plasmid
    #214895
    Purpose
    Expresses equimolar Lipid Droplet- and Lysosome- targeted split-FAST fragments for Short-range LD-LY Contact sites
    Depositor
    Tito Calì
    Insert
    SPLICSFAST-LDh-LY-P2A
    Tags
    none
    Expression
    Bacterial and Mammalian
    Available Since
    Feb. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    214895-plasmid-map-sequence-id-423481

    Enlarge

  16. pBHSTC104

    Plasmid
    #124356
    Purpose
    Plasmid expressing N- and C-terminal split fragments of gp41-1 intein inserted into CcdA antitoxin
    Depositor
    Hideo Iwai
    Article
    Beyer et al Chembiochem. 2019 Apr 8. doi: 10.1002/cb
    Insert
    gp41-1 N-intein and C-intein (bicistronic)
    Tags
    CcdA-C (SFAD)
    Expression
    Bacterial
    Promoter
    STC
    Available Since
    April 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    124356-plasmid-map-sequence-id-242355

    Enlarge

  18. CAPN1

    Plasmid
    #25607
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    CAPN1 (CAPN1 Human)
    Tags
    His
    Expression
    Bacterial
    Mutation
    Contains amino acids 29-360
    Available Since
    Oct. 25, 2011
    Availability
    Industry, Academic Institutions, and Nonprofits

  25. SPLICSFAST-PO-MT-P2A

    Plasmid
    #214899
    Purpose
    Expresses equimolar Peroxisome- and Mitochondria- targeted split-FAST fragments for Short-range PO-MT Contact sites
    Depositor
    Tito Calì
    Insert
    SPLICSFAST-PO-MT-P2A
    Tags
    none
    Expression
    Bacterial and Mammalian
    Available Since
    Feb. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    214899-plasmid-map-sequence-id-423486

    Enlarge

  28. pJK511

    Plasmid
    #155384
    Purpose
    dCas12a oscillator, reverse direction crRNAs. dCas12a (D917A) + PA4-mVenus
    Depositor
    Pamela Silver
    Article
    Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81
    Inserts
    dCas12a (F. novicida)
    PA4-mVenus
    dCas12a oscillator crRNAs
    Use
    CRISPR
    Expression
    Bacterial
    Mutation
    D917A (nuclease-deactivating)
    Available Since
    Dec. 8, 2020
    Availability
    Academic Institutions and Nonprofits only
    155384-plasmid-map-sequence-id-309004

    Enlarge

  32. pET24a-VHH-tev-mCherry

    Plasmid
    #117752
    Purpose
    Bacterial expression of a functionalized anti-GFP (VHH) nanobody fused to mCherry. VHH-mCherry also contains a T7, HA, BAP, tev cleavage site and His6 epitope
    Depositor
    Martin Spiess
    Article
    Buser et al J Vis Exp. 2019 Feb 21;(144). doi: 10.37
    Insert
    anti-GFP nanobody fused to a T7, tev site, mCherry, HA, BAP and His6 epitope
    Tags
    BAP, HA, His6, and T7
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Feb. 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    117752-plasmid-map-sequence-id-235994

    Enlarge

  34. RAB27B

    Plasmid
    #25545
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    RAB27B (RAB27B Human)
    Tags
    His
    Expression
    Bacterial
    Available Since
    Aug. 25, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  35. pET26b-BsaI

    Plasmid
    #233523
    Purpose
    Purification vector with BsaI cut sites for streamlined cloning
    Depositor
    Carl Denard
    Type
    Empty backbone
    Tags
    6xHis-tag and pelB leader
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    July 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    233523-plasmid-map-sequence-id-474147

    Enlarge

  36. pAcHELIX-sgRNA_entry (CJT94)

    Plasmid
    #181783
    Purpose
    Expresses AcHELIX containing a nicking I-AniI fusion to AcTnsB. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).
    Depositor
    Benjamin Kleinstiver
    Article
    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038
    Insert
    nAniI-AcTnsB, AcTnsC, AcTniQ, AcCas12k
    Expression
    Bacterial
    Mutation
    nAniI = K227M, F80K, L232K
    Promoter
    Lac and J23119
    Available Since
    March 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    181783-plasmid-map-sequence-id-358223

    Enlarge

Showing: 4801 - 4840 of 32069 results