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MYST3
Plasmid#25181PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertMYST3_13 (KAT6A Human)
UseTagsHisExpressionBacterialMutationcontains catalytic region ( residues 497-780).PromoterAvailable sinceOct. 25, 2011AvailabilityIndustry, Academic Institutions, and Nonprofits -
pRSF-FGE
Plasmid#102615PurposeInducible expression of Formylglycine-generating enzymeDepositorInsertFormylglycine-generating enzyme
UseTagsExpressionBacterialMutationPromoterT7Available sinceNov. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGEX Syt1 C2AB (96-421)
Plasmid#170643PurposeExpression of syt1 for purification in E. ColiDepositorInsertSYT1 (Syt1 Rat)
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET51b(+)-SNAP-CLIP
Plasmid#127180PurposeBacterial expression of the fused self-labeling tags SNAP-tag and CLIP-tagDepositorInsertSNAP-CLIP
UseTagsHisTag and StrepTagExpressionBacterialMutationPromoterT7Available sinceJune 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSC-IntE2
Plasmid#52265PurposeBacterial expression of GST-tagged TARGET proteins fused to SUMO-E2 with GyrA intein linkerDepositorInsertUbc9 GST fusion (UBE2I Human, Synthetic)
UseTagsGST and GyrA-inteinExpressionBacterialMutationPromoterAvailable sinceJuly 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pF709-pET28a-Hs-FL-ETFbeta-NHis wt
Plasmid#85110Purposeexpression of recombinant human full-length ETFbeta wt with N-terminal 6xHis-tagDepositorInserthuman full-length ETFbeta (ETFB Human)
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceNov. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPSU40
Plasmid#173831PurposeExpresses 40 kD Penn State ladder proteinDepositorInsertHSTPABPACGST
UseTagsExpressionBacterialMutationPromoterT7Available sinceAug. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBK086
Plasmid#183145PurposepET-6xHis-MBP-TEV-MapTIR-APAZ/MapAgo - Heterolgous MapSPARTA expressionDepositorInsertMapSPARTA operon (6xHis-MBP-MapTIR-APAZ and MapAgo)
UseTags6xHis and MBPExpressionBacterialMutationPromoterT7Available sinceApril 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pEERM4 Cm
Plasmid#64026PurposePlasmid for integration into neutral site 2 of Synechocystis 6803DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterPnrsBAvailable sinceMay 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pET-APOBEC1-YTHmut
Plasmid#200159PurposeFor bacterial expression of the APOBEC1-YTHmut fusion protein for protein purificationDepositorInsertAPOBEC1-YTHmut
UseTags6xHis, HA, and MBPExpressionBacterialMutationCodon optimized for E coli expressionPromoterLacZAvailable sinceJune 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAWP78-PVCpnf_FLAG-pvc2
Plasmid#198285PurposeContains an external epitope tag on the sheath protein; for immunofluorescence-based binding assaysDepositorInsertPVCpnf_FLAG-pvc2
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28a-GGG-ELP(120nm)-Cys
Plasmid#91572PurposeElastin Like Polypeptide: [(VPGEG)-(VPGVG)4-(VPGAG)2-(VPGGG)2-(VPGEG)]6, with N-Terminal Sortase Recognition Sequence GGG and a C-terminal CysteineDepositorInsertGGG-ELP(120nm)-Cys
UseTagsCysteine and Sortase-TagExpressionBacterialMutationPromoterT7Available sinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pET30b-7Y-SrtA
Plasmid#105601Purposeexpresses 7Y sortase A variant in E.coliDepositorInsertS.aureus Sortase A, 7Y mutant
UseTagsHis tagExpressionBacterialMutationPromoterT7Available sinceJan. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC57-codon-modified HTK
Plasmid#34563DepositorInsertcodon-modified HTK
UseTagsExpressionBacterialMutationcodon modified HTK using mouse codon usage tablePromoterpUC57 bacteria promoterAvailable sinceJan. 9, 2012AvailabilityAcademic Institutions and Nonprofits only -
GST-FOXO3a WT
Plasmid#1790DepositorInsertFOXO3a (FOXO3 Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceDec. 8, 2005AvailabilityAcademic Institutions and Nonprofits only -
pPBC-LG6s
Plasmid#62809PurposepPBC-LG6s expresses PiggyBac inverted terminal repeat-flanked Lck-GCaMP6s under the control of the CAG promoter.DepositorInsertITR-CAG-Lck-GCaMP6s-ITR
UseTagsExpressionBacterial and MammalianMutationPromoterCAGAvailable sinceMay 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
TS_pLuxLac
Plasmid#140426PurposeToggle switch circuit responsive to AHL gradient.DepositorInsertsGFP
LacI
TetR
mCherry
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJune 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAT220
Plasmid#134888PurposeBacterial expressionDepositorInserteGFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJuly 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCRT7 NT Topo tetR/pLtetO Amp‐WT sfGFP
Plasmid#52053PurposeUsed in phosphoprotein synthesis. Replace the sfGFP with gene of interest (GOI). Add TAG amber codon(s) at the position(s) in GOI where phosphoserine incorporation is desired.DepositorInsertsfGFP
UseTagsExpressionBacterialMutationPromotertetR/pLtetOAvailable sinceApril 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCS-PesaRlux
Plasmid#47640Purposewt esaR promoter in pCS26DepositorInsertPesaR
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPesaRAvailable sinceOct. 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
pQTEV-RPL9
Plasmid#31334DepositorInsertribosomal protein L9 (RPL9 Human)
UseTagsHis and TEVExpressionBacterialMutationPromoterAvailable sinceMarch 25, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBAD/HisB-Fast-FT
Plasmid#31916DepositorInsertFast-FT
UseTagsHisExpressionBacterialMutationM18V/E34K/K69R/L84W/S149T/A224S compared to mCher…PromoteraraBAvailable sinceSept. 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
pKAR2-Br512g3.2
Plasmid#171201PurposeExpresses Br512g3.2 polymerase in E.coliDepositorInsertEngineered Bst DNA Polymerase I Large Fragment
UseTags8x His tag; Villin Headpiece 47 amino acid (vHP47…ExpressionBacterialMutationPromoterT7Available sinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAWP78-PVCpnf_pvc13-HA
Plasmid#198296PurposeProduces PVCs retargeted with HA tagDepositorInsertPVCpnf_pvc13-HA
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pProEx Rab1A(S20C) CL
Plasmid#224272PurposeProtein purification of Rab1A (S20C)DepositorInsertRab1A (RAB1A Human)
UseTagsExpressionBacterialMutationS20CPromoterAvailable sinceDec. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLY071f
Plasmid#227942PurposeSICLOPPS precursor for cyclo-CRCVFSGDepositorInsertEvolved Ssp SICLOPPS-CRCVFSG
UseTagsExpressionBacterialMutationEvolved Ssp split inteins with multiple mutations…PromoterAvailable sinceNov. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
T. aq RNA Polymerase
Plasmid#215349PurposeExpress T. aq RNA Polymerase subunits in E. coli cellsDepositorInsertRNA polymerase
UseTags6x HisExpressionBacterialMutationPromoterAvailable sinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
SSH-pSUMO-α-COPI-WD40
Plasmid#208341PurposeBacterial expression plasmid for Schizosaccharomyces pombe α-COPI-WD40DepositorInsertN-terminal WD40 domain of yeast α-COPI (1-327) with substitutions (Leu181Lys, Leu185Lys, Ile192Lys, Leu196Lys and Phe197Lys)
UseTagsHis-tag, SUMO-tag, and Strep-tagExpressionBacterialMutationLeu181Lys, Leu185Lys, Ile192Lys, Leu196Lys and Ph…PromoterAvailable sinceJune 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
SPLICSFAST-NU-MT-P2A
Plasmid#214898PurposeExpresses equimolar Nucleus- and Mitochondria- targeted split-FAST fragments for Short-range NU-MT Contact sitesDepositorInsertSPLICSFAST-NU-MT-P2A
UseTagsnoneExpressionBacterial and MammalianMutationPromoterAvailable sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBad33_ISEc41_reRNA
Plasmid#205341PurposeBacterial expression of ISEc41 TnpB and its reRNADepositorInsertISEc41 TnpB
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBad33_ISEc46_reRNA
Plasmid#205343PurposeBacterial expression of ISEc46 TnpB and its reRNADepositorInsertISEc46 TnpB
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBad33_ISNsp2_reRNA
Plasmid#205350PurposeBacterial expression of ISNsp2 TnpB and its reRNADepositorInsertISNsp2 TnpB
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_SKAP2-1/1
Plasmid#91447PurposeProtein expression and purification of human SH3 domain construct SKAP2-1/1DepositorInsertSKAP2-1/1 (SKAP2 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTR47m4
Plasmid#104068PurposeLuciferase Reporter for Formaldehyde SensingDepositorInsertsxluxAB
frmR
frm Promoter m4
UseLuciferase and Synthetic BiologyTagsExpressionBacterialMutation23r and 25th nucleotides in sequence mutated from…PromoterAvailable sinceMarch 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a(+)_6xHIS-hHLA-A2 (MHC Class I heavy)-C-Tag
Plasmid#187356PurposeBacterial expression and refolding of gp100 pMHCDepositorInserthHLA-A2 (MHC Class I heavy) with N-terminal 6xHIS tag, and C-terminal C-Tag
UseTagsExpressionBacterialMutationPromoterT7Available sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only
