32,669 results
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Plasmid#224928PurposeTransposition assayDepositorInsertLE_cargo_RE
ExpressionBacterialAvailable SinceApril 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRSET FLII12Pglu-700uDelta6
Plasmid#13568DepositorInsertFLIPglu F16A Y12S-ECFP-D13R, 6aa linker
TagsCitrineExpressionBacterialMutationF16A, ECFP insert in mglBAvailable SinceFeb. 7, 2008AvailabilityAcademic Institutions and Nonprofits only -
18 pCOLI_A_Trx
Plasmid#160255PurposeN-term His-Thioredoxin 3C cleavable Amp resistant bacterial expression vectorDepositorTypeEmpty backboneTags6xHis and ThioredoxinExpressionBacterialPromoterT7Available SinceDec. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBAD_dGFPmut3-AAV
Plasmid#187379Purposearabinose-inducible expression of destabilized GFPmut3 (containing a AAV C-terminal tail)DepositorInsertdGFPmut3_AAV
TagsAAVExpressionBacterialMutationC-terminal AAV tailAvailable SinceAug. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
HECTD1
Plasmid#32860PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertHECTD1 (HECTD1 Human)
TagsHis (with TEV cleavage)ExpressionBacterialMutationamino acids 1879-1966 onlyAvailable SinceJan. 6, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBSV2_PresT-mCherryBb
Plasmid#118238PurposeKanamycin-resistant E.coli/B. burgdorferi shuttle vector; Encodes resT promoter driven mCherryDepositorInsertresT promoter driven mCherry
ExpressionBacterialAvailable SinceJan. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
PGR-Blue
Plasmid#68374PurposeTerminator quantification vector utilizing Golden Gate Assembly and amilCP. Positive colonies are green and false positives are blue. Terminators are placed between GFP and RFP - induced by arabinose.DepositorTypeEmpty backboneUseSynthetic Biology; Golden gate assemblyExpressionBacterialPromoterpBADAvailable SinceOct. 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
TRBV6-5
Plasmid#123425Purposeconstruction of TCRDepositorAvailable SinceMay 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
KLHL40 (KBTBD5A-c006)
Plasmid#98233PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceSept. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pE1n
Plasmid#17441Purposemodified pENTR vector for N-terminal STREP tag fusion with your gene of interestDepositorTypeEmpty backboneUseModified gateway entry vectorTagsSTREPExpressionBacterial, Insect, Mammalia…Available SinceMarch 21, 2008AvailabilityAcademic Institutions and Nonprofits only -
Yellow Cameleon-Nano15/pBig
Plasmid#51962PurposeExpression of Yellow Cameleon-Nano15, an ultrasensitive Ca2+ indicator with Kd = 15 nM, in DictyosteliumDepositorInsertYellow Cameleon-Nano15
ExpressionBacterialAvailable SinceApril 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRSET-B CFP
Plasmid#108858PurposeEncodes for the fluorescent protein CFPDepositorInsertCFP
ExpressionBacterialAvailable SinceApril 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28C-mCherry-53BP1LCD
Plasmid#204407PurposeE. coli expression of the 53BP1 low-complexity domain (1208-1977) with an N-terminal mCherry tagDepositorAvailable SinceSept. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAT593
Plasmid#68909PurposeNanoLuc expressed constiutively from PBT1311 and rpiL* RBS, pNBU2 backbone, AmpRDepositorInsertNanoLuc
ExpressionBacterialAvailable SinceOct. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pUC18T-mini-Tn7T-hph-Para
Plasmid#218363PurposeEmpty backbone for Tn7-site insertion with araC-ParaBAD expression systemDepositorTypeEmpty backboneExpressionBacterialAvailable SinceJune 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKEW302-AcrVA4
Plasmid#115656PurposeExpresses AcrVA4 in bacteria, under TetR control.DepositorInsertAcrVA4
UseSynthetic BiologyExpressionBacterialPromoterPtetAvailable SinceFeb. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
TRBV14
Plasmid#123449Purposeconstruction of TCRDepositorAvailable SinceMay 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
TRBV2
Plasmid#123409Purposeconstruction of TCRDepositorAvailable SinceMay 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
TRAV19
Plasmid#123391Purposeconstruction of TCRDepositorAvailable SinceMay 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
TRAV8-4
Plasmid#123377Purposeconstruction of TCRDepositorAvailable SinceMay 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.3TOPO-T7-hCas9
Plasmid#161876PurposeIn vitro transcription of SpCas9DepositorInserthuman codon optimized SpCas9
ExpressionBacterial and MammalianPromoterCMVAvailable SinceSept. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPP076
Plasmid#158794PurposepRSF-Duet1 derived plasmid containing C-terminally hexa-histidine tagged CasΦ-1 in MCS1 for expression in E. coli and protein purificationDepositorInsertCasPhi-1
ExpressionBacterialAvailable SinceAug. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUS212
Plasmid#84580PurposeE.coli cloning and expression vector. Has cloning sites downstream of Tet promoter.DepositorTypeEmpty backboneExpressionBacterialAvailable SinceNov. 21, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
pPBO.ACT006
Plasmid#182716PurposeConstituitvely expressed CasRx crRNA cloning vector, truncated 3' terminator region, with eGFP for cloningDepositorInsertCasRx crRNA cloning backbone
UseCRISPRTagsGFP in cloning site of crRNA for easier screening…ExpressionBacterialMutationCatalytically deactivated R295A, H300A, R849A, H8…PromoterpJ23119 (TTGACAGCTAGCTCAGTCCTAGGTATAATACTAGT)Available SinceAug. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSF18
Plasmid#222593PurposeBB2 ZWF1 tet-on overexpression cassetteDepositorArticleInsertZWF1
ExpressionBacterialMutationInternal BsaI and BpiI sites removed from insertPromotertet-onAvailable SinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pACYCDuet1-tglB
Plasmid#134676PurposeExpresses His6-TglBin E. ColiDepositorInsertTglB
TagsHis6ExpressionBacterialPromoterT7Available SinceJuly 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEX 4T1 hBad
Plasmid#35567DepositorAvailable SinceMarch 26, 2012AvailabilityAcademic Institutions and Nonprofits only -
pDEST14-SpyCatcher003-TEVs-SpyTag003DA
Plasmid#216283PurposeExpresses SpyCatcher003-TEV site-SpyTag003DA in bacterial cytoplasm, to allow TEV protease to remove SpyTag003DA and thereby uncage SpyCatcher003 reactivity.DepositorInsertSpyCatcher003-TEVs-SpyTag003DA
TagsHis6 and TEV cleavage siteExpressionBacterialPromoterT7 promoterAvailable SinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLVX-RT006-GCN4-HaloPM
Plasmid#163386PurposeTo express 4X sGCN4 module in the G-baToN systemDepositorInsert4XGCN4-Halotag-PDGFRtm-2A-mCherry (GCN4-HaloPM)
UseLentiviralTagsmCherryExpressionBacterial and MammalianPromoterEF1aAvailable SinceFeb. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pOKD5
Plasmid#17215DepositorTypeEmpty backboneExpressionBacterialAvailable SinceJan. 10, 2008AvailabilityAcademic Institutions and Nonprofits only -
pSW068
Plasmid#140036PurposeKO of PCC 11901 fadD gene/expresses codon-optimized 'tesA gene from E. coli from inducible cLac143 promoterDepositorInsertTruncated thioesterase gene
ExpressionBacterialPromotercLac143Available SinceApril 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTAT2.1
Plasmid#35620DepositorTypeEmpty backboneTags6xHIS and TATExpressionBacterialPromoterT7Available SinceMarch 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
pJK647
Plasmid#71701PurposeProduces E. coli thioredoxin reductase (EcTrxB)DepositorInsertthioredoxin reductase
ExpressionBacterialPromoterT7Available SinceJan. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
A1-LCD deltahexa
Plasmid#169714PurposeBacterial Expression of human hnRNPA1-A Low complexity domain including amino acids 186-320, hexapeptide including aa 259-264 deletedDepositorInserthnRNPA1 (HNRNPA1 Human)
Tags6xHisExpressionBacterialMutationhnRNPA1-A Low complexity domain including amino a…PromoterT7Available SinceJune 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJB02
Plasmid#111959PurposeExpresses GST-GFP-DHFR; construct generated for pull-down and localization experimentsDepositorInsertsmEGFP
eDHFR
TagsDHFRExpressionBacterialMutationeDHFR;residues 2-159 and enhanced GFP with monome…PromoterT7Available SinceAug. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
MP3
Plasmid#69650PurposeUntargetted Bacterial MutagenesisDepositorInsertdnaQ926 dam
ExpressionBacterialPromoterE. coli PBADAvailable SinceSept. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6P1-Vinculin tail
Plasmid#107154Purposebacterial expressionDepositorAvailable SinceMarch 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
Kindlin
Plasmid#186135PurposeFor expression of recombinant kindlin in bacteriaDepositorAvailable SinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_VASP_WH1
Plasmid#109790PurposeProtein expression and purification of VASP_WH1DepositorInsertVASP_WH1 (VASP Human)
ExpressionBacterialAvailable SinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
"AND-gate" receiver plasmid
Plasmid#127676PurposeEncodes the transcriptional activator LuxR and GFP. LuxR has an pLlacO-1 promoter and the GFP has a pLux promoter.DepositorInsertLuxR, GFP
UseSynthetic BiologyExpressionBacterialPromoterpLlacO-1 for LuxR, pLux for GFPAvailable SinceJuly 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
IRE-SunTag
Plasmid#196920PurposeThe 5UTR of the ferritin heavy chain mRNA was cloned upstream of the SunTag-Renilla coding sequence in the ΔIRE-SunTag reporterDepositorInsertRenilla luciferase
ExpressionBacterial and MammalianAvailable SinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFLxR5
Plasmid#149482PurposeBroad-Host range expression vector, RSF1010 origin, LuxR activator, PLuxB promoter, mRFP, Gentamicin Resistance Marker.DepositorInsertLuxR-mRFP
UseSynthetic BiologyExpressionBacterialPromoterPLuxBAvailable SinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBlu-CGT-1
Plasmid#170158PurposeExpresses bacterial Cyclomaltodextrin Glucanotransferase from Paenibacillus sp. c36 in E.coliDepositorInsertCyclomaltodextrin Glucanotransferase
ExpressionBacterialPromoterTacAvailable SinceMay 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMBP-HRV3C-AcrIF9
Plasmid#141442PurposeExpression of AcrIF9 in E. coli, tagged with an HRV3C-cleavage fusion to Maltose Binding Protein.DepositorInsertAcrIF9
TagsHRV3C cleavage site and Maltose Binding ProteinExpressionBacterialPromoterptacAvailable SinceMay 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
BB2_AB
Plasmid#98534Purposeempty BB2 for transcription unit cloningDepositorTypeEmpty backboneExpressionBacterialAvailable SinceFeb. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
p006-GFP-weak
Plasmid#108314PurposeGFP from jellyfish Aquorea sp. under weak synthetic constitutive promoter pFAB4282; kanamycin resistant.DepositorInsertGreen fluorescent protein
ExpressionBacterialPromoterpFAB4282Available SinceSept. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTorPE-jREX-GECO1
Plasmid#113941PurposeBacterial expression of red fluorescent Ca2+ sensor jREX-GECO1DepositorInsertjREX-GECO1
Tags6xHis and Modified TorA MNNNDLFQASRRRFLAQLG[G to …ExpressionBacterialAvailable SinceDec. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGST2-GST-TEV-OPTN
Plasmid#198428PurposeUsed for the expression and purification of OPTN (WT) (SMC Internal No. 1587)DepositorAvailable SinceApril 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJUMP49-2E(sfGFP)
Plasmid#126995PurposeLevel 2 vector. OriV 9 (pBBR322/ROP; medium copy number); sfGFP cloning reporter.DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialMutationNoneAvailable SinceJune 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pU6T7
Plasmid#71462PurposeExpresses gRNA from hybrid human U6/T7 promoter for both cellular expression and in vitro transcription from same promoter (2 Gs at initiation)DepositorTypeEmpty backboneUseCRISPRExpressionBacterial and MammalianPromoterhuman U6/phage T7Available SinceFeb. 9, 2016AvailabilityAcademic Institutions and Nonprofits only