29,771 results
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Plasmid#35276DepositorInsertRFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterBADAvailable sinceFeb. 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
csr-1_son-1_mTagBFP2
Plasmid#191746PurposeTest fluorescent properties in Neurospora crassa. Nuclear envelope fluorescent reporter.DepositorInsertNCU04288 (NCU04288 Neurospora crassa)
UseTagsNCU04502 promoter and mTagBFP2ExpressionBacterialMutationPromoterAvailable sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCJH019_SC101_pLAC_Cas12c(4)_Kan
Plasmid#183071PurposeWild-type Cas12c_4 in a plasmid containing a SC101 origin and lac promoter for in vivo interference assay.DepositorInsertCas12c_4
UseTagsExpressionBacterialMutationWild-typePromoterAvailable sinceJune 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pZSm45 ndhII
Plasmid#48889PurposeLux-regulated expression of NDH-2 enzymeDepositorInsertndhII and LuxR
UseSynthetic BiologyTagsssrA tag (AANDENYALAA)ExpressionBacterialMutationPromoterpLuxAvailable sinceFeb. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDSG291
Plasmid#115605Purposesurface-displayed Null, used in composability experiments; low copyDepositorInsertregulated adhesin construct
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMLM36 (aka pBR-UV5-GP-FD2)
Plasmid#21871DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 3, 2009AvailabilityAcademic Institutions and Nonprofits only -
pOSIP-TH (TetR, HK022)
Plasmid#45988DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJuly 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
1257 pGEX4T3 GST FKHR
Plasmid#10706DepositorInsertFKHR (FOXO1 Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceMarch 14, 2006AvailabilityAcademic Institutions and Nonprofits only -
pET-2(KW)2-eGFP
Plasmid#112736PurposeExpress 2(KW)2-eGFP in E. coliDepositorInsert2(KW)2-eGFP
UseTags6xHisExpressionBacterialMutationPromoterT7 PromoterAvailable sinceJuly 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTrc99S-ssDsbA-EPA-DNNNS-DQNRT
Plasmid#128404PurposePeriplasmic expression of P. aeruginosa exotoxin A with DNNNS and DQNRT internal glycosylation sites in E. coliDepositorInsertExotoxin A (toxA P. aeruginosa)
UseTags6xHis tag and DsbA signal sequence for periplasmi…ExpressionBacterialMutation242DNNNS246 and 384DQNRT388 engineered glycosylat…PromotertrcAvailable sinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRiboI-Sec-APEX2m
Plasmid#176845PurposeExpression of APEX2 in the periplasm from a codon-optimized gene (single-copy integrating plasmid)DepositorInsertpRibo-Sec-APEX2m
UseTagsExpressionBacterialMutationPromoterpRibo, theophilline riboswitch inducible hsp60 pr…Available sinceJan. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pOSV801
Plasmid#126044Purposemodular integrative vector for the assembly of DNA fragments and expression in Streptomyces speciesDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pQE80_T5_Z18-ehaA_LacI
Plasmid#186314PurposePlasmid for inducible E coli expression of the Z18 helixCAM.DepositorInsertZ18-ehaA
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJPC12-ΔM13-mCherry-P/PM,5C6A-GFP
Plasmid#80910PurposeReporter plasmid (mCherry and GFP)DepositorInsertsmCherry
GFP
UseTagsExpressionBacterialMutationPromoterP5C6A and PM,5C6AAvailable sinceDec. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAB045a
Plasmid#79224PurposeDirected Evolution of Protein-Protein InteractionsDepositorInsertPlacZ rpoZ-HA4; PlacIq lacI
UseTagsExpressionBacterialMutationPromoterPlacZ; PlacIqAvailable sinceOct. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCI-T7Max-UTR1-deGFP-8xHis-T500
Plasmid#178422PurposeExpression of deGFP via a T7 Promoter in TXTLDepositorInsertdeGFP
UseSynthetic BiologyTags8xHis TagExpressionBacterialMutationPromoterT7MaxAvailable sinceDec. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBS hTSP-3
Plasmid#12995DepositorInsertTSP-3 (THBS3 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 28, 2006AvailabilityAcademic Institutions and Nonprofits only -
pACM4-ACA
Plasmid#49809PurposeEncodes fabD, accA, accB, accC, accD in pseudo-operon configuration.DepositorInsertsACP transacylase
Acetyl-CoA carboxylase
acetyl-CoA carboxylase
Acetyl-CoA carboxylase
Acetyl-CoA carboxylase
UseTagsExpressionBacterialMutationSilent site-directed mutagenesis to remove SalI s…PromoterT7Available sinceFeb. 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBluescript GPX-1
Plasmid#13038DepositorInsertGPX-1 (GPX1 Human)
UseTagsExpressionBacterialMutationAdditional GCG upstream of codon 11. Codon 92 is …PromoterAvailable sinceNov. 7, 2006AvailabilityAcademic Institutions and Nonprofits only -
HC078 (3xHA-Atg13)
Plasmid#59544PurposeExpresses yeast Atg13 under the endogenous promoter with a 3xHA N-terminal tagDepositorInsertATG13 (ATG13 Budding Yeast)
UseTags3xHAExpressionBacterial and YeastMutationAvrII site created after start codonPromoterATG13Available sinceOct. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
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pCDF-NtXNtS1R1R2B2
Plasmid#160882PurposeExpression of N. tabacum Rubisco small subunit (rbcS-S1), Rubisco chaperone protiens rbcX, raf1, bsd2 and raf2 in E. coli.DepositorInsertrbcS-S1, rbcX, raf1, raf2, bsd2
UseTagsExpressionBacterialMutationPromoterrbcS-S1: T7, chaperones: T7Available sinceDec. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcNB2
Plasmid#89242PurposeResurfaced Cell-penetrating 5F7 nanobodyDepositorInsertpcNB2 (5F7)
UseTagsExpressionBacterialMutationPromoterT7Available sinceOct. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
EC71173
Plasmid#154061PurposeLevel 1, Position 2 Golden Gate vector. HspHv17::Cre-U5-CreDepositorInsertCre recombinase
UseTagsExpressionBacterialMutationIntroduction of U5 intron sequences, to prevent p…PromoterHvHSP17Available sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pAB094e
Plasmid#79245PurposeDirected Evolution of Protein-Protein InteractionsDepositorInsertPBAD rpoZ-HA4 (Y87A); PC araC
UseTagsExpressionBacterialMutationPromoterPBAD; PCAvailable sinceMay 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pOPS0263
Plasmid#133228PurposeReporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.DepositorInsertconstructed with PsNifH (pOGG043), gusA (pOGG083) and T-pharma (pOGG003)
UseTagsExpressionBacterialMutationDomesticated for Golden Gate cloningPromoterAvailable sinceApril 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pOPS0262
Plasmid#133227PurposeReporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.DepositorInsertconstructed with PsNifH (pOGG043), celB (pOGG050) and T-pharma (pOGG003)
UseTagsExpressionBacterialMutationDomesticated for Golden Gate cloningPromoterAvailable sinceApril 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pB1H1-Zif268
Plasmid#12612DepositorInsertZif268 in pB1H1 (Egr1 Mouse)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 3, 2006AvailabilityAcademic Institutions and Nonprofits only -
pETDuet RagA(T21N)-RagC
Plasmid#99644PurposeBacterial expression of RagA(T21N)-RagCDepositorUseTagsHis tagExpressionBacterialMutationT21NPromoterAvailable sinceNov. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRset-OppA
Plasmid#207869PurposeBacterial expression of poly-his-tagged L. lactic OppADepositorInsertOppA
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceOct. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
Q-IL18-6
Plasmid#166554PurposescFv of a human scaffold targeting Interleukin-18. Antigen coverage aa 37-193 of 193, full-length excl. signal peptideDepositorInsertsingle chain-Fv, scFv
UseRecombinant antibody expressionTags3xFLAG, Sortag, His6 and OmpA leader sequenceExpressionBacterialMutationPromoterLacZAvailable sinceApril 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSLAX13 (CT#232)
Plasmid#13918DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 9, 2007AvailabilityAcademic Institutions and Nonprofits only -
pJ23119-sgRNA
Plasmid#113654PurposesgRNA targeting unit plasmid. The sgRNA targeting unit plasmids contain connector ConLS, ConR1, and one sgRNA transcriptional unit.DepositorInsertpromoter J23119 and sgRNA
UseCRISPRTagsExpressionBacterialMutationPromoterJ23119Available sinceSept. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
PARD3_PDZ_3
Plasmid#103959PurposeProtein expression and purification of PAR3 PDZ3 domainDepositorInsertPARD3 (PARD3 Human)
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET22b-GFP-CPDSalI
Plasmid#38257DepositorInserteGFP
UseTags6xHis and Vibrio cholerae MARTX toxin cysteine pr…ExpressionBacterialMutationcloned from pEGFPN3 (Clonetech)PromoterT7Available sinceJuly 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
pBTK564
Plasmid#110613PurposeBTK assembled broad-host-range plasmid encoding NanoLuc luciferase protein driven by PA1 promoterDepositorInsertNanoluc
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
bfloGFPa1
Plasmid#60749PurposeBacterial expression of Amphioxus GFPa1DepositorInsertGFPa1
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceOct. 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET28-iLOV-Rsn2
Plasmid#61687Purposebacterial expression of ranaspumin 2 as a fusion protein with the fluorescent tag iLOVDepositorInsertRanaspumin-2
UseTagsHis and iLOVExpressionBacterialMutationPromoterT7Available sinceMarch 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pKM154
Plasmid#13036DepositorInsertcat, sacB
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 7, 2006AvailabilityAcademic Institutions and Nonprofits only -
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pET-3c-rhVTN-NC
Plasmid#30226DepositorInsertrhVitronectin with N and C terminus cleavages (VTN Human)
UseTagsExpressionBacterialMutationdeleted amino acids: 399-478, 20-62PromoterAvailable sinceSept. 26, 2011AvailabilityAcademic Institutions and Nonprofits only -
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pBR322-Pdp1_NTD-ZFD_R
Plasmid#198277PurposeFor loading R half of zinc finger deaminase targeting human TRAC locus (ZFD_TRAC-NC-Right-G1397-C_DddA); also contains PVC regulatory genesDepositorInsertPdp1_NTD-ZFD_R
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pC0065 Pin2Cas13 (B09) His6-TwinStrep-SUMO-BsaI
Plasmid#115216PurposeExpresses Pin2Cas13b from a SUMO-tagged bacterial expression constructDepositorInsertPin2Cas13
UseTagsSUMOExpressionBacterialMutationPromoterAvailable sinceSept. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
C1-82 pComb3xss anti-cFos nanobody
Plasmid#145829PurposeBacterial expression of anti-cFos (Human) recombinant llama nanobody for use as an immunolabelDepositorInsertanti-cFos (Human) recombinant llama nanobody C1-82 (FOS L. glama (llama))
UseTags6xHis-HAExpressionBacterialMutationPromoterlacAvailable sinceJune 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAB078d9
Plasmid#79207PurposeDirected Evolution of Protein-Protein InteractionsDepositorInsertPlacZ-opt (OR1+2+3) luxAB; Ppro1 434cI-SH2ABL1
UseTagsExpressionBacterialMutationPromoterPlacZ-opt (OR1+2+3); Ppro1Available sinceAug. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
TOPO Cre-2A-GFP
Plasmid#68450PurposeTOPO construct expressing Cre-2A-GFP for generation of GMAP-compatible geneDepositorInsertCre-2A-GFP
UseGmapTagsExpressionBacterialMutationPromoterPlacAvailable sinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET30b_T7Sav
Plasmid#138588PurposeT7-tagged streptavidin with native C-terminus controlled by PT7 promoter; KanR, pBR322 oriDepositorInsertT7-tagged streptavidin variant with native C-terminus
UseSynthetic BiologyTagsT7-tagExpressionBacterialMutationPromoterPT7Available sinceMarch 11, 2020AvailabilityAcademic Institutions and Nonprofits only