32,803 results
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Plasmid#174667PurposeExpresses GFP-endoNA2 fusion protein for detection of polysialic acid. The endoNA2 is a catalytically inactive form of endosialidase from bacteriophage PK1A2.DepositorInsertGFP-endoNA2 fusion protein
TagsHistagExpressionBacterialPromoterT5Available SinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSW002-Pc-mNeonGreen
Plasmid#205016PurposeBroad host-range bacterial expression vector with constitutive Pc promoter; for tagging bacteria with mNeonGreen (codon optimized for P. fluorescens)DepositorInsertmNeonGreen
ExpressionBacterialMutationmNeonGreen is codon optimized for expression in P…PromoterPcAvailable SinceFeb. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSBY1_FnCpf1cg
Plasmid#104622PurposeCRISPR system used to genome editing in Mycobacterium smegmatis, expresses Fn CPf1DepositorInsertFnCpf1
UseCRISPRExpressionBacterialMutationcodon-optimized C.glutamicumPromoterhsp60Available SinceJune 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103 YAP1 WW domain #2
Plasmid#104228PurposeBacterial expression of WW domain #2 from YAP1DepositorInsertYAP1 WW domain #2 (YAP1 Human)
TagsGSTExpressionBacterialMutationcodon optimized for expression in bacteriaPromotertacAvailable SinceFeb. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103 YAP1 WW domain #1
Plasmid#104227PurposeBacterial expression of WW domain #1 from YAP1DepositorInsertYAP1 WW domain #1 (YAP1 Human)
TagsGSTExpressionBacterialMutationcodon optimized for expression in bacteriaPromotertacAvailable SinceJan. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBacPuroR-NeoR
Plasmid#34921DepositorInsertsBacterial promoter::PuroR::bacterial 3p flank
Bacterial promoter::NeoR::bacterial 3p flank
ExpressionBacterialAvailable SinceMay 4, 2012AvailabilityAcademic Institutions and Nonprofits only -
pAB076i3
Plasmid#79198PurposeDirected Evolution of Protein-Protein InteractionsDepositorInsertPlacZ-opt (OR1) gIII, luxAB; Ppro1 434cI-SH2ABL1
ExpressionBacterialPromoterPlacZ-opt (OR1); Ppro1Available SinceApril 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28-His6-Ubc9
Plasmid#133909PurposeBacterial expression of His6-Ubc9DepositorInsertSUMO-conjugating enzyme UBC9 (UBE2I Human)
TagsThrombin-cleavable His6-tagExpressionBacterialPromoterT7Available SinceSept. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAB151
Plasmid#101666PurposeOpto-T7RNAP*(563-F1)DepositorInsertOpto-T7RNAP*(563-F1)
UseSynthetic BiologyExpressionBacterialAvailable SinceSept. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLVX-Tight-Puro_MYRF
Plasmid#64843PurposeA lentiviral vector for the expression of mouse MYRF under doxycyline control.DepositorAvailable SinceMay 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pIA028
Plasmid#200419PurposethrC::(PAmCherry1 erm2),for PAmCherry1 fusions.PALM integration vector for B. subtilis. Includes PAmCherry1 photoactivatable FP with multi cloning sites (MCS) for fast and interchangeable cloningDepositorTypeEmpty backboneTagsPAmCherry1ExpressionBacterialAvailable SinceAug. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET29_LuxSit_i
Plasmid#194299PurposeBacterial expression of the de novo luciferase, LuxSit-iDepositorInsertLuxSit-i
ExpressionBacterialAvailable SinceMarch 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDEST17-UbE2G1
Plasmid#15790DepositorAvailable SinceSept. 28, 2007AvailabilityAcademic Institutions and Nonprofits only -
pCA1.332 pDUOTK1-L1
Plasmid#162351PurposeLevel 0 terminator acceptor containing dual fluorophores for testing transcription terminator efficiencyDepositorInsertseYFP
LacZ alpha
mTagBFP
UseSynthetic BiologyExpressionBacterialAvailable SinceFeb. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLG034 (restriction-like)
Plasmid#157912PurposeExpresses a restriction-like defense systemDepositorInsert4-gene restriction-like system
ExpressionBacterialAvailable SinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET19b+H3
Plasmid#157807PurposeExpression of cysteine-free human histone H3 (c111A)DepositorInsertH3
ExpressionBacterialMutationC111APromoterT7Available SinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMflT-o4
Plasmid#101312PurposeContains: complete oriC region of M. florum L1 (oriC4: rpmH/dnaA and dnaA/dnaN intergenic regions, with dnaA), colE1 rep origin, recoded tetM resistance gene, origin of transfer of RP4 plasmid.DepositorInsertoriC4 of M. florum strain L1 (rpmH/dnaA and dnaA/dnaN intergenic regions, with dnaA)
UseSynthetic BiologyExpressionBacterialAvailable SinceNov. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTKEI-Mal-B2
Plasmid#79756PurposeSingle fluorescent protein biosensor for maltoseDepositorInsertMal-B2
Tags6xHisExpressionBacterialPromoterpTrcAvailable SinceJuly 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_PLK1_Pkinase
Plasmid#109933PurposeProtein expression and purification of PLK1_PkinaseDepositorInsertPLK1_Pkinase
ExpressionBacterialAvailable SinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pH6HTC_RAN-Halo
Plasmid#175339PurposeProduction of HaloTag fusion proteinDepositorAvailable SinceOct. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRSET.HaloTag.v7.AviTag
Plasmid#195486PurposeBacterial expression plasmid with Halotag v7 insert plus a C-terminus appended AviTag for enzymatic biotinylation.DepositorInsertHaloTag.v7.AviTag
Tags6xHIS and AviTagExpressionBacterialPromoterT7Available SinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLG026 (tmn)
Plasmid#157904PurposeExpresses the tmn defense systemDepositorInserttmn (transmembrane ATPase)
ExpressionBacterialAvailable SinceNov. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1_Cre
Plasmid#201198PurposeAAV vector for cre expression under EF1 promoterDepositorInsertscre
EF1alpha promoter
UseAAVExpressionBacterial and MammalianMutationnuclear targeting crePromoterN/A and hEF1alpha promoterAvailable SinceJuly 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET-3c-rhVTN-N
Plasmid#30225DepositorInsertrhVitronectin with N terminus cleavage (VTN Human)
ExpressionBacterialMutationdeleted amino acids: 20*-62Available SinceMay 25, 2011AvailabilityAcademic Institutions and Nonprofits only -
pETDUET-GST-AUP1 379-410:Ube2G2
Plasmid#185335PurposeUsed for co-expression of the AUP1 G2BR with UBE2G2 for crystallographyDepositorAvailable SinceFeb. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBRC901
Plasmid#167948Purposeexpress wild-type bacterial tyrosine kinase Wzc from E. coli E69 (O9a:K30)DepositorInsertwild-type bacterial tyrosine kinase Wzc from E. coli E69 (O9a:K30)
Tagshexa-histidine tagExpressionBacterialPromoteraraBADAvailable SinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pVH015
Plasmid#80398PurposeContains pBAD-CusR (response regulator) and pCusR-antiscaffold-GFP(3xFLAG). Used for Western blotsDepositorUseSynthetic BiologyTagsC-terminal 3xFLAG tag and C-terminal LZx domainExpressionBacterialAvailable SinceSept. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
HisMBP CFlm25
Plasmid#78458PurposeInducible expression in E. coliDepositorInsertHuman Cleavage Factor lm 25 kDa subunit (NUDT21 Human)
TagsHis and MBPExpressionBacterialPromotertacAvailable SinceJuly 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET21d-colEwt2/Imwt2
Plasmid#107127Purposeplasmid for expression of WT colicin endonuclease and immunity pair 2DepositorInsertcolEwt2/Imwt2
TagsHis-tagExpressionBacterialPromoterT7Available SinceNov. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTOX5
Plasmid#127450PurposeEncodes MqsR toxin and amilCP chromoprotein. CAM-RDepositorInsertsmqsR toxin
amilCP chromoprotein
ExpressionBacterialMutationCodon optimized from protein sequence in FPbasePromoterpRha and tacAvailable SinceJuly 12, 2019AvailabilityAcademic Institutions and Nonprofits only