31,059 results
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Plasmid#41689DepositorInsertTvo VMA intein
TagsGB1 and His-tagExpressionBacterialPromoterT7Available SinceJan. 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET22b 6His SpyCatcher-GRFT
Plasmid#204502PurposeBacterial expression of GRFT with N-terminal SpyCatcher fusion and GS linkerDepositorInsertGRFT
UseSynthetic BiologyTags6xHis and SpyCatcherExpressionBacterialPromoterT7Available SinceSept. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_ESRRG_Hormone-recep
Plasmid#109910PurposeProtein expression and purification of ESRRG_Hormone-recepDepositorInsertESRRG_Hormone-recep (ESRRG Human)
ExpressionBacterialAvailable SinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTD103luxI_sfGFP -R
Plasmid#48887PurposePositive feedback plasmid from oscillator without luxRDepositorInsertsfGFP and luxI
UseSynthetic BiologyTagsssrA tag (AANDENYALAA)ExpressionBacterialPromoterpLuxAvailable SinceFeb. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBbA8c-RFP
Plasmid#35272DepositorInsertRFP
UseSynthetic BiologyExpressionBacterialPromoterBADAvailable SinceFeb. 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
pH
Plasmid#64178Purposeharbours marker gene HIS3DepositorTypeEmpty backboneExpressionBacterial and YeastAvailable SinceSept. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
CelTag Plasmid
Plasmid#66562PurposeEncodes CelTag for the tagging and high affinity purification of endogenous yeast proteins.DepositorInsertCelTag
ExpressionBacterialAvailable SinceFeb. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pETconNK-TEM1(S70A,D179G)
Plasmid#81166PurposepETconNK with TEM1(S70A,D179G) beta-lactamaseDepositorInsertTEM-1 beta-lactamase
Tagsc-mycExpressionBacterial and YeastMutationDeleted the sec transport tag. Plasmid encodes H2…PromoterGAL1 and gal1Available SinceFeb. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
SPI1_pet28a
Plasmid#131653PurposeBacterial expression of ETS gene SPI1DepositorAvailable SinceOct. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pANTO5
Plasmid#131106PurposeTo generate an integrative plasmid carrying the TetR/Pip-OFF system, but not the integraseDepositorInserttetR gene by pFRA61 (Boldrin et al 2010)
ExpressionBacterialAvailable SinceDec. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET15b/GgVcl 1-1066 (full length)
Plasmid#46171DepositorAvailable SinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBLO 4.01
Plasmid#121495Purposeengineered Mu transposonDepositorTypeEmpty backboneExpressionBacterialPromoterp15AAvailable SinceMarch 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pSrpR-S3
Plasmid#74693PurposeSrpR-S3 gateDepositorInsertPTac-RBSS3-SrpR-PSrpR-YFP
ExpressionBacterialAvailable SinceApril 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046871
Plasmid#145724PurposeBacterial Expression plasmid for SARS-CoV-2 nsp10DepositorInsertSARS-CoV-2 nsp10 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
TagsCleavable TEV;6xHISExpressionBacterialMutationEscherichia coli recode 1PromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available SinceApril 27, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pKB226
Plasmid#170016PurposeEncodes LacI-controlled expression of surface-displayed defensin HNP-1 and PvirK-mNeonGreen reporterDepositorArticleInsertsmNeonGreen
Defensin HNP-1
UseSynthetic BiologyTagsLpp, OmpA, and TetherExpressionBacterialPromoterPtac and PvirKAvailable SinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pVE2_11_p40S_QfDBD_VP16AD_cp_phl_DsRed_0xQUAS
Plasmid#154229PurposeEncodes p40S:QfDBD-VP16AD-GFP-NLS synthetic transcription factor, activating DsRed-SKL with promoter CP_phl (no QUAS control)DepositorInsertp40S:QfDBD-VP16AD-GFP-NLS
UseSynthetic Biology; Fungal expressionTagsGFP-NLS; DsRed-SKLExpressionBacterialPromoterp40S (AN0465 A. nidulans)Available SinceJuly 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
S5_EcoRV_Ef1a_AscI
Plasmid#128469PurposeEf1a promoterDepositorInsertEf1a promoter
ExpressionBacterialAvailable SinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET15bLCFPT7
Plasmid#53545PurposeActivation module. The plasmid consists of a mutant T7 RNA polymerase (T7 RNAP) activating its own expression via a T7 promoter carrying alac operator. Carbenicillin resistantDepositorInsertT7 RNAP
ExpressionBacterialAvailable SinceJuly 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET28a His6Flag2AUF1(p37)
Plasmid#135939PurposeExpresses AUF1p37 in E. coliDepositorInsertheterogeneous nuclear ribonucleoprotein D (HNRNPD Human)
TagsFlag2 and His6ExpressionBacterialPromoterT7Available SinceJan. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTricHis-PLY
Plasmid#160537PurposeExpression of inserted PLY variantDepositorInsertPLY-WT
TagsHis-TagExpressionBacterialAvailable SinceJan. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBTK509
Plasmid#110610PurposeBTK assembled broad-host-range plasmid encoding GFP driven by PA2 promoterDepositorInsertGFP
ExpressionBacterialAvailable SinceAug. 21, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSb_init containing anti SARS-CoV-2_RBD sybody Sb#14
Plasmid#153522PurposeExpression of the anti SARS-CoV-2_RBD sybody Sb#14DepositorInsertSb#14
ExpressionBacterialPromoterpBADAvailable SinceAug. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pC239
Plasmid#65953PurposeN2 inverter plasmid for two-strain systemDepositorInsertsAiiA
LacI
TagsLAA taggedExpressionBacterialPromoterpCin and pCin*Available SinceAug. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pETM33_CALM1_EF-hand
Plasmid#178468PurposeBacterial expression of human domain with His-tag and GST tagDepositorAvailable SinceMarch 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEX SHP-2(N)-SH2
Plasmid#46498DepositorInsertSHP-2(N) (PTPN11 Human)
TagsGST and PreScissionExpressionBacterialMutationcontains amino acids 1-104PromoterTacAvailable SinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGEX-Nedd-4/2522
Plasmid#11466DepositorAvailable SinceApril 5, 2006AvailabilityAcademic Institutions and Nonprofits only -
JpExpress404 PTEN
Plasmid#49420PurposeBacterial Expression VectorDepositorAvailable SinceAug. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046809
Plasmid#145685PurposeBacterial Expression plasmid for SARS-CoV-2 2'-O-ribose methyltransferaseDepositorInsertSARS-CoV-2 2'-O-ribose methyltransferase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
TagsCleavable TEV;6xHISExpressionBacterialMutationwtPromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available SinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAC1750
Plasmid#127984PurposeAMA1 plasmid with Aspergillus optimized Cpf1, ble selection marker, and USER cassette (PacI/Nt.BbvCI)DepositorInsertCpf1
UseCRISPR; Fungal expressionTagsSV40 NLSExpressionBacterialMutationcodon optimized for Aspergillus nidulansPromoterAspergillus nidulans tef1 promoterAvailable SinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
RP1B FUS 1-163 QQ4xSS #1
Plasmid#127194PurposeProtein expression for affinity purificationDepositorInsertFUS 1-163 QQ4xSS #1 (FUS Human)
TagshexahistidineExpressionBacterialMutationQQ4xSS #1: Q27S, Q28S, Q93S, Q94S, Q132S, Q133S, …Available SinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBAD-pHoran4
Plasmid#61558PurposeOrange fluorescent protein based pH indicator in bacterial expression vectorDepositorInsertpHoran4
Tags6xHisExpressionBacterialAvailable SinceDec. 19, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGEX 4T1 RARalpha
Plasmid#35557DepositorAvailable SinceApril 5, 2012AvailabilityAcademic Institutions and Nonprofits only -
pDUET-ctCPR-A17AMO
Plasmid#20115DepositorInsertp450 reductase and amorphadiene oxidase
UseSynthetic BiologyExpressionBacterialAvailable SinceFeb. 27, 2009AvailabilityAcademic Institutions and Nonprofits only -
m2-26 mTRF2
Plasmid#12300DepositorInsertmTRF2 (Terf2 )
ExpressionBacterialAvailable SinceAug. 11, 2006AvailabilityAcademic Institutions and Nonprofits only -
pUC-UAS1B4-Leum
Plasmid#44312DepositorInsertUAS1B4-Leum promoter
UseSynthetic BiologyExpressionBacterialMutationN/APromoterUAS1B4-LeumAvailable SinceJuly 15, 2013AvailabilityIndustry, Academic Institutions, and Nonprofits -
pJJS009
Plasmid#188332PurposePCR template for repair templates to tag a gene with a mKate2 and mIAA7 AID degron using homology-directed repair for C. elegans.DepositorInsertmIAA7::mKate2::Linker
ExpressionBacterialAvailable SinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
CoV-2-NSP3-c001
Plasmid#173083PurposeExpression of recombinant fusion protein in E. coliDepositorInsertrep (ORF1ab Severe acute respiratory syndrome coronavirus 2 (2019-nCoV) (SARS-CoV-2))
TagsmCeruleanExpressionBacterialMutationE1084-E1192, codon optimizedAvailable SinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
hsp70:mKate2-NWASP-CA
Plasmid#105967Purposeheat shock inducible transgenesisDepositorInsertNWASP-CA
TagsmKate2ExpressionBacterialAvailable SinceFeb. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
NpT7-5-Erk2
Plasmid#39230DepositorAvailable SinceJuly 15, 2013AvailabilityAcademic Institutions and Nonprofits only -
Fyn-SH3_5WT_S25C
Plasmid#118824PurposeBacterial expression of the SH3 domain of human FynDepositorInsertFYN (FYN Human)
Tags6-His-TEV tagExpressionBacterialMutationS25C; See depositor comments below.Available SinceApril 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET22b-EC12WTC9A
Plasmid#47924PurposeExpresses E-cadherin EC12 WT with C9A mutation in E.Coli.DepositorInsertE-Cadherin EC12
Tags6xHis and TEV CleavageExpressionBacterialMutationchanged Cysteine 9 to AlaninePromoterT7Available SinceSept. 27, 2013AvailabilityAcademic Institutions and Nonprofits only -
pJSC281 - Bacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9), REC2 FRET variant
Plasmid#101230PurposeBacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9), REC2 FRET variantDepositorInsertSpCas9 variant C80S/C574S/E60C/D273C/N692A/M694A/Q695A/H698A
Tags10x His, MBP, and TEV siteExpressionBacterialMutationC80S, C574S, E60C, D273C, N692A, M694A, Q695A and…PromoterT7Available SinceNov. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEE065
Plasmid#176822PurposeEncodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expressionDepositorInsert704
Tags6xHisExpressionBacterialPromoterT7Available SinceNov. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pGBW-m4046731
Plasmid#145633PurposeBacterial Expression plasmid for SARS-CoV-2 nsp2DepositorInsertSARS-CoV-2 nsp2 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
TagsCleavable TEV;6xHISExpressionBacterialMutationEscherichia coli recode 1PromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available SinceApril 28, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
GABPA_pet28a
Plasmid#131640PurposeBacterial expression of ETS gene GABPADepositorAvailable SinceFeb. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SUMO-SpyTag003
Plasmid#216282PurposeExpresses SUMO with a C-terminal SpyTag003 in bacterial cellsDepositorInsertSUMO-SpyTag003
TagsHis6 and SpyTag003ExpressionBacterialPromoterT7 promoterAvailable SinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNIC-HIS6-THRX-TEV-GG
Plasmid#215796PurposeCloning of target gene with N terminal His6 Thioredoxin tag and TEV cleavageDepositorTypeEmpty backboneExpressionBacterialAvailable SinceApril 25, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pHH0103_CSK-1/1
Plasmid#91542PurposeProtein expression and purification of human SH3 domain construct CSK-1/1DepositorInsertCSK-1/1 (CSK Human)
ExpressionBacterialAvailable SinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only