32,672 results
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Plasmid#16346DepositorInsertPEA3 (Etv4 Mouse)
ExpressionBacterialAvailable SinceFeb. 4, 2009AvailabilityAcademic Institutions and Nonprofits only -
pBAD-TrxRFP1
Plasmid#115327PurposeBacterial expression of TrxRFP1DepositorInsertTrxRFP1
TagsHis-TagExpressionBacterialPromoterpBADAvailable SinceSept. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pHAGE2-TetOminiCMV-cMyc
Plasmid#136614PurposeDox-inducible lentiviral vector expressing mouse cMycDepositorAvailable SinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4092694
Plasmid#153907PurposeBacterial expression plasmid for fragment SARS-CoV-2 orf1a mature peptide 3 to orf1a stopDepositorInsertfragment SARS-CoV-2 orf1a mature peptide 3 to orf1a stop (ORF1ab Severe acute respiratory syndrome coronavirus 2)
ExpressionBacterialMutationwtPromoterPromoter | t7 consensus from NEB, G is +1Available SinceJune 12, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
KIF3A406
Plasmid#129768PurposeExpresses mouse Kinesin-2 KIF3A head and neck linker, dimerized through kinesin-1 neck-coil to 406 for biochemical assaysDepositorInsertKIF3A (Kif3a Mouse)
TagsHis6ExpressionBacterialMutationTruncation at 359, fused to kinesin-1 starting Al…Available SinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
E. coli expression: Z-lock cofilin dark state mutant
Plasmid#141135Purposea control for optogenetic cofilin analogDepositorInsertZdk2, Cofilin, LOV2
ExpressionBacterialMutationD3 deletion, I32F in Zdk2Available SinceJan. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
BPTF
Plasmid#39111PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET3d::strep-AID
Plasmid#15924DepositorAvailable SinceOct. 26, 2007AvailabilityAcademic Institutions and Nonprofits only -
pET_BGL2
Plasmid#101911PurposeExpresses a β-glucosidase gene from Thermobaculum terrenum in E. coli under the control of the pET system.DepositorInsertβ-glucosidase
UseSynthetic BiologyExpressionBacterialPromoterT7+LacOAvailable SinceMarch 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSEVA12TEC-I
Plasmid#58308PurposeGeneGuard genomic cassette: for constructing ΔthyA::RBS I repA, Epsilon, FRT-kanR-FRT (medium-copy support for ColE2 COR). Will support pSEVAT7Z.DepositorInsertGenomic cassette
UseSynthetic BiologyExpressionBacterialAvailable SinceMarch 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFREE2-SpCas9-sgRNA
Plasmid#179582PurposeExpresses spCas9 and gRNA that targets ColE1 plasmids for curingDepositorInsertCas9
ExpressionBacterialAvailable SinceMarch 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRC3EGFP
Plasmid#193790PurposeExpresses EGFP under the control of PR promoter, p15A origin of replication, Chloramphenicol selectionDepositorInsertEGFP
ExpressionBacterialPromoterPR promoterAvailable SinceApril 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHBJT001
Plasmid#225152PurposeHigh copy cloning vector for integration of C-terminal FLAG epitope tag. SmaI restriction site immediately upstream of the FLAG epitope. FLAG epitope in same orientation as lac promoter.DepositorTypeEmpty backboneUseSynthetic BiologyTagsFLAG epitope tag (35 bp)ExpressionBacterialAvailable SinceSept. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-51b(+)-Caprola_13
Plasmid#194674PurposeProtein production of the split HaloTag based calcium recorder Caprola_13 in bacteriaDepositorInsertCaprola_13
TagsHis-tag and Strep-tagExpressionBacterialPromoterT7Available SinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDL17_Cas9-His Δcys M1C
Plasmid#206251PurposeBacterial expression of SpCas9 Δcys M1C variant under T7 promoterDepositorInsertSpCas9
UseCRISPRTags6x-His and SV40 NLSExpressionBacterialMutationM1C, C80S, C574SPromoterT7Available SinceSept. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
His Puc (aa 1-393)
Plasmid#52295PurposeBacterial expression of His tagged Drosophila Puc fragmentDepositorAvailable SinceAug. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRU1100
Plasmid#14465DepositorInsertunstable gfp-mut3.1 LVA
ExpressionBacterialAvailable SinceApril 23, 2007AvailabilityAcademic Institutions and Nonprofits only -
pET30-2-PSPH
Plasmid#83909Purposestable overexpressionDepositorAvailable SinceOct. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEX_hDDX5_FL_D248N
Plasmid#88872Purposeinducible expression of GST-DDX5 D248N in bacteriaDepositorAvailable SinceJune 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
PET-3a-chpBK
Plasmid#155201PurposeTo express recombinant ChpBK proteinDepositorInsertschpS-chpBK-6×His-Tag
GyrA-CBD-10xHis-Tag
Tags10xHis and 6xHisExpressionBacterialAvailable SinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAH-CTX1-rhadCas9-native
Plasmid#129392PurposeDerived from pAH-CTX1-rha. The native Streptococcus pyogenes dCas9 gene cloned downstream of PrhaBAD. Non-codon-optimized dCas9 version of pAH-CTX1-rhadCas9.DepositorInsertS. pyogenes dCas9
UseCRISPRExpressionBacterialMutationAspartate 10 to Alanine, Histidine 840 to AlaninePromoterPrhaBADAvailable SinceDec. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTE103
Plasmid#59398PurposeMethyloBrick expression vector, fumC promoter; TcRDepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialPromoterPfumCAvailable SinceDec. 30, 2014AvailabilityAcademic Institutions and Nonprofits only -
pJKR-L-cdaR
Plasmid#62558PurposeGlucarate biosensor. Low copy plasmid that expresses GFP proportional to glucarate concentration.DepositorInsertGFP
ExpressionBacterialAvailable SinceAug. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTHSSe_53
Plasmid#109251PurposepUC BbsI cloning vectorDepositorInsertsfGFP
ExpressionBacterialPromoterPJ23105Available SinceMay 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET14b PHAS-I
Plasmid#15679DepositorInsertPHAS-I (Eif4ebp1 Rat)
Tags6xHisExpressionBacterialMutationNucleotides 1-354 of rat PHAS-I plus 450bp of 3…Available SinceSept. 24, 2007AvailabilityAcademic Institutions and Nonprofits only -
RRAS2
Plasmid#38816PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
TTR C10S S46C
Plasmid#190008PurposeExpression of human TTR in E coliDepositorAvailable SinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBEL1994
Plasmid#139898PurposeExpression plasmid for LetB PLL2 deletion (without TM region)DepositorInsertLetB
ExpressionBacterialMutationLetB(43-877; delta229-239, D228G, L240G)-6xHisAvailable SinceSept. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
Pf-ClpP
Plasmid#25591PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertPf-ClpP
TagsHisExpressionBacterialAvailable SinceAug. 25, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
pUK
Plasmid#64185Purposeharbours marker genes URA3 and LYS2DepositorTypeEmpty backboneExpressionBacterial and YeastAvailable SinceAug. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
SpyCas9(WT)
Plasmid#101044PurposeExpress Streptococcus pyogenes Cas9DepositorInsertSpyCas9
TagsGSTExpressionBacterialAvailable SinceOct. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
ALC1-c007
Plasmid#173112PurposeExpression of recombinant fusion protein in E. coliDepositorAvailable SinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
MYST1
Plasmid#25180PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceAug. 9, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET28a_LolT_C41S
Plasmid#211789PurposeExpression plasmid in E. coli BL21(DE3) , which can be used for expression and purification to get protein LolT_C41SDepositorInsertlolt mutant
TagsHis tagExpressionBacterialPromoterT7 promoterAvailable SinceJan. 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBTK1004 (pSL3)
Plasmid#190999PurposeExpresses red chromoprotein on a broad host range RSF1010 origin plasmidDepositorInsertred chromoprotein
UseSynthetic BiologyExpressionBacterialPromoterCP25Available SinceOct. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET22b-GFP-CPDSalI
Plasmid#38257DepositorInserteGFP
Tags6xHis and Vibrio cholerae MARTX toxin cysteine pr…ExpressionBacterialMutationcloned from pEGFPN3 (Clonetech)PromoterT7Available SinceJuly 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
GPX3
Plasmid#38899PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSK+ chickBMPR-1B DN
Plasmid#49529Purposecloning vector for dominant negative chick BMP receptor 1BDepositorInsertBMPR-1B DN (BMPR1B Chicken)
ExpressionBacterialMutationdominant negative mutation (K231R); also P2A, M11…PromoterT3Available SinceMay 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBEL2511
Plasmid#195625PurposeComplementation of MlaA(WT)DepositorInsertMlaA
ExpressionBacterialAvailable SinceMay 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
A1-LCD+7R+12D
Plasmid#178678PurposeBacterial expression of N-terminally 6His tagged A1-LCD with seven Arg residues added, twelve Glu residues added (LCD: low-complexity domain, aa 186-320)DepositorInsertA1-LCD+7R+12D (HNRNPA1 Human)
Tags6xHisExpressionBacterialMutationseven Arg residues added, twelve Glu residues add…Available SinceMarch 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
mt TEFM
Plasmid#205058PurposeTEFM protein expressionDepositorAvailable SinceFeb. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC18T-mini-Tn7T-Zeo-ecfp
Plasmid#65038PurposeZeoR mini-Tn7 vector, mobilizable, for CFP tagging bacteriaDepositorInsertecfp
ExpressionBacterialAvailable SinceNov. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET-(pos36)GFP-9xGGS-Cre-6xHis
Plasmid#62938PurposeExpression of (+36)GFP-GGS9-Cre-6xHis in bacterial cellsDepositorInsert(ps36)GFP-9xGGS-Cre
Tags(pos36)GFP and 6xHis tagExpressionBacterialPromoterT7Available SinceMay 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCfuNca-Plastid
Plasmid#190818PurposePlastid targeting GFP with ble resistant gene for zeocinDepositorInsertsDiatom plastid targeted GFP
Bleomycin resistance gene
ExpressionBacterialPromoterCylindrotheca fusiformis fcp and Nitzschia captiv…Available SinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
APneg-SNAP25d
Plasmid#168014Purposenegative selection PACE Accessory PlasmidDepositorInsertSNAP25-T7PAP
ExpressionBacterialAvailable SinceMay 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
DT035A
Plasmid#159397PurposeConstitutive superfolder GFP (sfGFP) expression, controlled under E.coli tac promoterDepositorInsertpr.tac-sfGFP
UseSynthetic BiologyTagsFLAGExpressionBacterialPromotertac promoterAvailable SinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDSP32
Plasmid#199374PurposeEncodes dual cistronic nuclear localized jGCaMP7s and mScarlet-I optimized for C. elegans expressionDepositorInsertNLS::jGCaMP7s::egl-13NLS::SL2::NLS::mScarlet-I::egl-13NLS
ExpressionBacterialMutationno mutations, except added subcellular localizati…Available SinceApril 25, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
p277_DG
Plasmid#234695PurposeGolden gate domesticated pET28b for scarless cloning with His6-3CDepositorTypeEmpty backboneTagsHis6-3CExpressionBacterialMutationNoAvailable SinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
p349_DG
Plasmid#234679PurposeGolden gate domesticated pET28b with His10-GST-3CDepositorTypeEmpty backboneTagsHis10-GST-3CExpressionBacterialMutationNoAvailable SinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only