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Plasmid#204642PurposeExpression of enAsCas12f in E. coliDepositorInsertenAsCas12f
UseTags6xHisExpressionBacterialMutationD196K, N199K, G276R, N328G, D364RPromoterT7Available sinceAug. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHO4d-Cas9
Plasmid#67881PurposeCas9nlsHis6 Bacterial Expression constructDepositorInsertCas9
UseCRISPRTagsHis6 and T7 epitopeExpressionBacterialMutationPromoterT7Available sinceSept. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET9d-hnRNP-A1
Plasmid#23026DepositorInserthnRNPA1 (HNRNPA1 Human)
UseTagsExpressionBacterialMutationPromoterT7Available sinceOct. 26, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCDF-1b-RFC140ΔN555
Plasmid#175043PurposeOverexpression of human (Rfc1) p140ΔN555 in E.coliDepositorInsertReplication factor C subunit 1 (RFC1 Human)
UseTagsExpressionBacterialMutationPromoterT7 promoterAvailable sinceJan. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRSET Nucleolin
Plasmid#13037DepositorInsertNucleolin (NCL Human)
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceOct. 5, 2006AvailabilityAcademic Institutions and Nonprofits only -
His6_MBP_FXR1P
Plasmid#198694PurposeExpression of human FXR1 protein in E. coliDepositorInsertFXR1P (FXR1 Human)
UseTagsMBPExpressionBacterialMutationPromoterT7Available sinceMay 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCI-neo-cOVA
Plasmid#25097DepositorInsertcytoplasmic ovalbumin
UseTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceJuly 1, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCasKP-hph
Plasmid#117232PurposeBacterial expression of Cas9 nuclease and lambda-Red system in Klebsiella PneumoniaeDepositorInsertCas9
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAP05
Plasmid#186261PurposesfGFP under light light responsive PEL222 promoter and EL222 under constitutively active BBa_J2305 promoterDepositorInsertsUseTagsExpressionBacterialMutationPromoterBBa_23105 (GGCTAGCTCAGTCCTAGGTACTATGCTAGC) and PE…Available sinceSept. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLVX-RT004-LaG30VB
Plasmid#163384Purpose2nd generation lentiviral vector to express anti-GFP(LaG30) module in the G-baToN systemDepositorInsertLaG30-VEEGFRtm-BFP (LaG30VB)
UseLentiviralTagsBFPExpressionBacterial and MammalianMutationPromoterEF1aAvailable sinceJan. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHN781
Plasmid#178828PurposeBacterial expression for human Saposin ADepositorInsertartificial gene encoding human Saposin A (AR Human)
UseTagsHis8, TEVExpressionBacterialMutationCodon-usage was optimized for bacterial expressio…PromoterT7Available sinceJan. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCpf1b-sp
Plasmid#122188PurposeBacterial genome editing plasmid with spectinomycin resistance marker and using sacB as a counter-selection markerDepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceAug. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
eSrtA(4S-9) pET29b
Plasmid#75146Purposereprogrammed sortase A variant that recognizes alternative substrate LPXSG with high activity and specificityDepositorInsertreprogrammed sortase A variant
UseTags6x HisExpressionBacterialMutationN98D S102C A104V A118T F122A K134R F144L I182V E1…PromoterT7Available sinceAug. 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET28a-MSP1D1deltaH4-H6
Plasmid#71717PurposeMembrane scaffold protein (MSP), helix 4 to 6 deletionDepositorInsertApolipoprotein A1 (APOA1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
human p53-(1-320)
Plasmid#24864DepositorInsertp53 (TP53 Human)
UseTagsHisExpressionBacterialMutationContains aa 1-320PromoterAvailable sinceAug. 23, 2010AvailabilityAcademic Institutions and Nonprofits only -
pMRE162
Plasmid#118510Purposebroad-host range plasmid to constitutively express fluorescent protein genes in bacteriaDepositorInsertsGFP2
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLVX-RT003-GPM
Plasmid#163383PurposeTo express sGFP module in the G-baToN systemDepositorInsertGFP-PDGFRtm-2A-mCherry (GPM)
UseLentiviralTagsmCherryExpressionBacterial and MammalianMutationPromoterEF1aAvailable sinceJan. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pOPINK-TRABID (OTU+AnkUBD, aa 245-697)
Plasmid#61590PurposeExpresses human TRABID (OTU+AnkUBD domains) in E. coli.DepositorInsertTRABID (ZRANB1 Human)
UseTagsHis6-GST-3CExpressionBacterialMutationDeleted aa 1-244 and aa 698-708.PromoterT7Available sinceMarch 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCpf1b
Plasmid#122187PurposeBacterial genome editing plasmid with kanamycin resistance marker and using sacB as a counter-selection markerDepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceMarch 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET21a - Ec-ClpX
Plasmid#111516PurposeExpresses E. coli ClpX with a C-terminal His tagDepositorInsertClpX
UseTagsHis tagExpressionBacterialMutationPromoterT7Available sinceJune 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMCSG53-DsbC
Plasmid#186623PurposeE. coli expression vector containing N-terminal leaderless DsbC-6His-TEV fusion.DepositorTypeEmpty backboneUseTagsDsbC-6His-TEVExpressionBacterialMutationPromoterT7Available sinceSept. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJL1-sfGFP-DQNAT
Plasmid#198210PurposepJL1 plasmid encoding superfolder GFP modified at the C-terminus with 30 amino acids containing an optimal DQNAT sequon followed by a 6x-His tagDepositorInsertsfGFP_DQNAT
UseTags6x His Tag and DQNAT glycosylation tagExpressionBacterialMutationPromoterT7Available sinceMay 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTECH-MbAcK3RS(IPYE)
Plasmid#104070PurposeExpression plasmid encoding MbAcK3RS(IPYE) and pylTDepositorInsertPlpp MbAcK3RS(IPYE); PproK pyl
UseTagsExpressionBacterialMutationContains activating mutations V31I, T56P, H62Y, A…PromoterPlpp and PproKAvailable sinceDec. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCpf1
Plasmid#122185PurposeBacterial genome editing plasmid with kanamycin resistance markerDepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceMarch 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEERM4 Cm
Plasmid#64026PurposePlasmid for integration into neutral site 2 of Synechocystis 6803DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterPnrsBAvailable sinceMay 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pCpf1-sp
Plasmid#122186PurposeBacterial genome editing plasmid with spectinomycin resistance markerDepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceMarch 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCS2+ centrin-mKate2
Plasmid#105941Purposein vitro transcription, zf centrin N-terminally tagged with mkate2DepositorInsertCentrin
UseTagsmKate2ExpressionBacterialMutationPromoterAvailable sinceFeb. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEERM3 Km
Plasmid#64025PurposePlasmid for integration into neutral site of Synechocystis 6803DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterPnrsBAvailable sinceMay 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET22b-csgA
Plasmid#89581PurposeExpress csgA curli protein with GS linker and HistaqDepositorInsertcsgA (csgA )
UseTags3xGGSG linker - TEV site - 3xGGSG linker and 6xHi…ExpressionBacterialMutationPromoterAvailable sinceMay 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJL1-CRM197-4xDQNAT
Plasmid#128395PurposeIn vitro expression of CRM197 with C terminal 4xDQNAT glycosylation sitesDepositorInsertDiphtheria toxin
UseTags4xDQNAT glycosylation tag and 6xHis tagExpressionBacterialMutationInactivating G52E mutationPromoterT7Available sinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMRE-Tn5-165
Plasmid#118545PurposeminiTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteriaDepositorInsertmScarlet-I
UseMinitn5 delivery vectorTagsExpressionBacterialMutationPromoterAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pNP1 sfGFP toehold switch
Plasmid#107355PurposeExpresses sfGFP (a GFP) in Ecoli off a T7 promoter when pNP1 sfGFP trigger is co-expressedDepositorInserttoehold 7 + sfGFP
UseTagsExpressionBacterialMutationPromoterT7Available sinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a-Rx3(A7)
Plasmid#35525DepositorInsertHis6-Rx3(A7)
UseTagsHis6ExpressionBacterialMutationChanged G2 to PPromoterT7Available sinceApril 5, 2012AvailabilityAcademic Institutions and Nonprofits only -
pT7-7 asyn S129E
Plasmid#36050DepositorInsertalpha-synuclein S129E (SNCA Human)
UseTagsExpressionBacterialMutationS129EPromoterT7Available sinceJuly 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
pZa-tetO-csgA-CmR
Plasmid#89579PurposeExpress csgA curli proteinDepositorInsertcsgA
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTF-ahpC-rfp
Plasmid#153035PurposeFor CRISPR-Cas12a genome editing in E. coli. Contains a constituitively expressed CRISPR array targeting the ahpC gene in E. coli and donor DNA to insert an rfp translational fusion in the ahpC gene.DepositorInsertCRISPR array
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTSAR3.5t
Plasmid#210257PurposeFluorescent reporter for Type III secretion system activity in Shigella flexneriDepositorInsertseBFP2
superfolder GFP
UseTagsssraExpressionBacterialMutationPromoteripaH7.8p and rpsMAvailable sinceFeb. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTSAR3.4t
Plasmid#210256PurposeFluorescent reporter for Type III secretion system activity in Shigella flexneriDepositorInsertsmCherry
superfolder GFP
UseTagsssraExpressionBacterialMutationPromoteripaH7.8p and rpsMAvailable sinceFeb. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET22b-csgB
Plasmid#89582PurposeExpress csgB curli protein with GS linker and HistaqDepositorInsertcsgB (csgB )
UseTags3xGGSG linker - TEV site - 3xGGSG linker and 6xHi…ExpressionBacterialMutationPromoterAvailable sinceMay 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
p403 – TDH3-MTS-mCherry-GFP1-10-His
Plasmid#177730PurposemCherry and the 1-10th β-strands of GFP (GFP1-10) fused to a mitochondria signal sequence for mitochondrial targeting in yeast plasmid with HIS markerDepositorInsertmCherry-GFP1-10
UseTagsMitochondrial Targeting SequenceExpressionBacterial and YeastMutationPromoterAvailable sinceJan. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pZa-tetO-csgB-CmR
Plasmid#89580PurposeExpress csgB curli proteinDepositorInsertcsgB
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCR4 hNFATc1-EE
Plasmid#17857DepositorInsertNFATc1 (NFATC1 Human)
UseTagsEEExpressionBacterialMutationPromoterAvailable sinceApril 29, 2008AvailabilityAcademic Institutions and Nonprofits only -
pJUMP21-1A(sfGFP)
Plasmid#126968PurposeLevel 1 vector. sfGFP reporter. Origin R6K (pir conditional).DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationNonePromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJUMP27ts-1A(sfGFP)
Plasmid#126975PurposeLevel 1 vector. sfGFP reporter. Thermosensitive origin pSC101 (low copy number)DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationRepA A56V thermosensitive mutationPromoterAvailable sinceJuly 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAIP4_3KT9
Plasmid#234192PurposeHeterologous protein expression of aprataxin in Escherichia coliDepositorInsert3KT9 (APTX Human)
UseTags10x HisTag-Smt3ExpressionBacterialMutationPromoterT7Available sinceMarch 6, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pTSAR3.1t
Plasmid#210254PurposeFluorescent reporter for Type III secretion system activity in Shigella flexneriDepositorInsertsmCerulean
superfolder GFP
UseTagsssraExpressionBacterialMutationPromoteripaH7.8p and rpsMAvailable sinceJune 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJL1-sfGFP-AQNAT
Plasmid#198211PurposepJL1 plasmid encoding superfolder GFP modified at the C-terminus with 30 amino acids containing a non-permissible AQNAT sequon followed by a 6x-His tagDepositorInsertsfGFP_AQNAT
UseTags6x His Tag and AQNAT mutated (non-permissible) gl…ExpressionBacterialMutationPromoterT7Available sinceMay 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJL1-sfGFP-MOOR
Plasmid#198212PurposepJL1 plasmid encoding superfolder GFP modified at the C-terminus with 32 amino acids containing the minimum optimal O-linked recognition site (MOOR) followed by a 6x-His tagDepositorInsertsfGFP_MOOR
UseTags6x His Tag and MOOR glycosylation tagExpressionBacterialMutationPromoterT7Available sinceMay 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKO403_lacZ_Sp
Plasmid#174726PurposeBifidobacterium-E.coli shuttle vector for seamless gene cloningDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only