32,684 results
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Plasmid#124244PurposeBacterial 6xHIS tagged protein expression without the addition of any potential phosphorylation sites to the tagged protein.DepositorTypeEmpty backboneExpressionBacterialAvailable SinceApril 4, 2019AvailabilityAcademic Institutions and Nonprofits only
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pGT2
Plasmid#13056DepositorTypeEmpty backboneExpressionBacterialAvailable SinceDec. 8, 2006AvailabilityAcademic Institutions and Nonprofits only -
6974 pPROEX-HTb-SSB1
Plasmid#1232DepositorAvailable SinceAug. 9, 2005AvailabilityAcademic Institutions and Nonprofits only -
SARS-CoV-2_N_deltaNTD
Plasmid#157869Purposeexpress MBP tagged SARS-CoV-2 Nucleocapsid protein without the N terminal domainDepositorAvailable SinceNov. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBC155
Plasmid#202274PurposeLevel 2 for strain-specific barcode DNA tag with bc182 through Tn7 bacterial insertion, Spectinomycin and Streptomycin selectable marker and tagBFP fluorescent markerDepositorInsertbc182
ExpressionBacterialAvailable SinceApril 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBC114
Plasmid#202276PurposeLevel 2 for strain-specific barcode DNA tag with bc141 through Tn7 bacterial insertion, Tetracyline selectable marker and far-red-FP fluorescent marker mPlumDepositorInsertbc141
ExpressionBacterialAvailable SinceApril 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC19-T7-Top-T
Plasmid#87309PurposeExpresses the "Top" transcript of the Split-Broccoli system under the control of the T7 promoter.DepositorInsertSplit-Broccoli system "Top" transcript
UseSynthetic BiologyExpressionBacterialPromoterT7Available SinceMay 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET21-10XHis-GST-HRV-dHer1cys
Plasmid#73218PurposeBacterial expression vector for Z1907 affibody protein (A) with N-terminal His10, GST and HRV3C cleavage site (cysteine tag)DepositorInsert10XHis-GST-HRV-dHer1cys
Tags10XHis-GST-HRV and The dimer of the Her1 Affibody…ExpressionBacterialPromoterT7Available SinceMarch 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
PTPRE
Plasmid#38950PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMay 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTricHis-PLY
Plasmid#160537PurposeExpression of inserted PLY variantDepositorInsertPLY-WT
TagsHis-TagExpressionBacterialAvailable SinceJan. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX-human MIZ1 wild type
Plasmid#74161Purposebacterial expression of MIZ1DepositorAvailable SinceApril 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET51b_SNAP-ecDHFR
Plasmid#187108PurposeExpression of SNAP-ecDHFR fusion in bacteriaDepositorInsertSNAP-ecDHFR
TagsHisx10 and StrepExpressionBacterialPromoterT7Available SinceJuly 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pShHELIX(Cas12k-TniQ)-sgRNA_entry (CJT111)
Plasmid#181790PurposeExpresses 3-component ShHELIX containing a Cas12k-TniQ fusion. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).DepositorInsertnAniI-ShTnsB, ShTnsC, ShCas12k-ShTniQ
ExpressionBacterialMutationnAniI = K227M, F80K, L232KPromoterLac and J23119Available SinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTYB2-eIF1
Plasmid#37217DepositorInserteIF1
ExpressionBacterialPromoterT7Available SinceJuly 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
pET3a-DJ1
Plasmid#51489PurposeInducible expression of human DJ1 in E.coliDepositorAvailable SinceMarch 31, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGEX4T3-HA-ERK2 D321N
Plasmid#53202PurposeExpresses GST and HA tagged human ERK2 D321N from bacterial expression vectorDepositorAvailable SinceJune 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAH-CTX1-rha
Plasmid#129390PurposeDerived from mini-CTX1. An integrase vector that can integrate the rhamnose-inducible promoter system into the chromosome of a host bacterium.DepositorInsertRhamnose-inducible promoter
ExpressionBacterialMutationN/APromoterPrhaBADAvailable SinceDec. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET41a iGluSnFR
Plasmid#119828PurposeProduction of recombinant iGluSnFR, a single-wavelength glutamate indicator, in E. coliDepositorInsertiGluSnFR
TagsGSTExpressionBacterialPromoterT7Available SinceApril 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-29
Plasmid#172753PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-29; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-29
Tags6xHIS and pelB leader for periplasmic secretionExpressionBacterialPromoterT7Available SinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRoRH
Plasmid#213133PurposeRiboptoregulator circuit RoRH for blue-light-activated gene expressionDepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialAvailable SinceJuly 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-45b-HOXD13(+7A)-IDR-mCherry
Plasmid#145281PurposeBacterial Expression of 6xHis HOXD13 (+7A) IDR mCherry Fusion ProteinDepositorAvailable SinceSept. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTetQCas-BsaI
Plasmid#170629PurposeExpression of CRISPR-associated transposasesDepositorInsertVchTniQ, VchCas8, VchCas7, VchCas6, CRISPR(BsaI)
ExpressionBacterialAvailable SinceAug. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
tIVR
Plasmid#122663PurposeExpress tIVR protein in E. coliDepositorInserttIVR
TagsHis-tagExpressionBacterialPromoterT7 promoterAvailable SinceJuly 17, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
As2
Plasmid#123160PurposeArsenic sensor used in the sensor cell array. pSB4A3 carrying J109-30arsR-t-ParsR-ABS84-30hrpR-30hrpS-t-PhrpLE-30gfp-tDepositorInsertJ109-30arsR-t-ParsR-ABS84-30hrpR-30hrpS-t-PhrpLE-30gfp-t
UseSynthetic BiologyExpressionBacterialPromoterJ109, ParsR-ABS84, PhrpLEAvailable SinceMay 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
DARS_pNIC-Bio3
Plasmid#153048PurposeExpression construct for producing Avi tagged Glycyl-tRNA synthetaseDepositorAvailable SinceJune 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
HK3
Plasmid#25303PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertHK3:R480-R922 (HK3 Human)
TagsHisExpressionBacterialMutationContains amino acids R480-R922Available SinceAug. 16, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
pIcaRA-I1
Plasmid#74682PurposeIcaRA-I1 gateDepositorInsertPTac-RBSI1-IcaRA-PIcaR-YFP
ExpressionBacterialAvailable SinceApril 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCDF-NtXNtS1R1AtR2NtB2
Plasmid#160881PurposeExpression of N. tabacum Rubisco small subunit (rbcS-S1), Rubisco chaperone protiens rbcX, raf1, bsd2 and A. thaliana Rubisco chaperone proteins raf2 in E. coli.DepositorInsertrbcS-S1, rbcX, raf1, raf2, bsd2
ExpressionBacterialPromoterrbcS-S1: T7, chaperones: T7Available SinceDec. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
CoV-2-NSP3-c001
Plasmid#173083PurposeExpression of recombinant fusion protein in E. coliDepositorInsertrep (ORF1ab Severe acute respiratory syndrome coronavirus 2 (2019-nCoV) (SARS-CoV-2))
TagsmCeruleanExpressionBacterialMutationE1084-E1192, codon optimizedAvailable SinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET-22b(+)-UTPase
Plasmid#12667DepositorAvailable SinceJan. 5, 2007AvailabilityAcademic Institutions and Nonprofits only -
pET3-ARL4C-His6
Plasmid#201869PurposeUsed for bacterial expression of human protein ARL4CDepositorAvailable SinceAug. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
TP2_crtYB
Plasmid#140233PurposeExpression of gene coding lycopene bifunctional β-cyclase for production beta-carotene from lycopeneDepositorInsertphytoene-beta carotene synthase
ExpressionBacterialMutationnaPromotert7 promoterAvailable SinceApril 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
LLhG-pHG165a E62K
Plasmid#90051PurposeConstitutive expression of a chimeric LacI:GalR transcription repressorDepositorInsertLLhG/E62K
ExpressionBacterialMutationE62KPromoterIqAvailable SinceJune 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
LacI-11/109-pHG165c
Plasmid#90059PurposeDimeric LacI (truncated last 11 amino acids) polymorphism with T at position 109DepositorInsertLacI-11/109
ExpressionBacterialMutation109PromoterIqAvailable SinceJune 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHybr_r2a>mG2a-srt-his
Plasmid#124803PurposeHDR template to knock-in mouse IgG2a Fc domain in rat IgG2a heavy chain locus. Use in combination with PX458-gR2A_ISO to convert rat IgG2a hybridoma to mouse IgG2a producing cell lines.DepositorInsertMurine IgG2a
UseCRISPR; Hdr templateTagsHistag (HHHHHH) and Sortag (LPETGG)ExpressionBacterial and MammalianAvailable SinceSept. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
GST-Cav FL (1-178)
Plasmid#22445DepositorInsertCaveolin1(1-178)
TagsGSTExpressionBacterialAvailable SinceDec. 8, 2009AvailabilityAcademic Institutions and Nonprofits only -
L1-R184Q
Plasmid#15501DepositorAvailable SinceOct. 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T-1 3xHA-MKK4-EE
Plasmid#47577PurposeBacterial expression plasmid for GST-3xHA-tagged constitutively active MKK4DepositorInsertMKK4-EE (MAP2K4 Human)
Tags3xHA and GSTExpressionBacterialMutationLacks the first 36 residues of the RefSeq protein…Available SinceAug. 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
BPV00554 pET21GG2-His(6)-BECN(248-450)
Plasmid#136685PurposeExpresses the Beclin-1 ECD/BARA domain (a.a. 248-450) with a N-terminal hexa-Histidine tagDepositorInsertBeclin-1 ECD/BARA (BECN1 Human)
TagsHexa-HistadineExpressionBacterialMutationDNA optimized for E. coli expressionPromoterT7Available SinceFeb. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGMCKq1-10M1-sspBmyc
Plasmid#27069DepositorInserttetR-PmyctetO-sspB
ExpressionBacterialAvailable SinceJune 22, 2011AvailabilityAcademic Institutions and Nonprofits only -
pMCSG7-H588A-NmeCas9
Plasmid#71477Purposebacterial pMCSG7 expression vector expressing Nme cas9 nickase with H588A mutation (HNH domain) with T7 promoter, N-terminal His tag and TEV siteDepositorInsertpMCSG7-H588A-NmeCas9
Tags6HisExpressionBacterialMutationH588APromoterT7Available SinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
RGS16
Plasmid#39140PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
Atg24 1B
Plasmid#197046PurposeHis6 tagged Atg24 from S. cerevisiae for expression in E. coli.DepositorInsertAtg24
Tags6His-TEVExpressionBacterialAvailable SinceJuly 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET24a HAB1 (172-511)
Plasmid#177844PurposeBacterial Expression of HAB1DepositorInsertProtein phosphatase 2C 16
Tags6xHis-GSTExpressionBacterialAvailable SinceDec. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSY14-ptb
Plasmid#90490PurposepSY8 derivative with sceC gene under the control of ptb promoter used for I-SceI-mediated scarless gene modification via allelic exchange in ClostridiumDepositorInsertsceC
ExpressionBacterialAvailable SinceMay 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRU1105
Plasmid#14469DepositorInsertDsRedT3
ExpressionBacterialAvailable SinceApril 23, 2007AvailabilityAcademic Institutions and Nonprofits only -
pPhlF-P2
Plasmid#74686PurposePhlF-P2 gateDepositorInsertPTac-RBSP2-PhlF-PPhlF-YFP
ExpressionBacterialAvailable SinceApril 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET22b-BirA-CPDSalI
Plasmid#38258DepositorInsertbirA (birA E. coli)
Tags6xHis and Vibrio cholerae MARTX toxin cysteine pr…ExpressionBacterialMutationcloned from pGEX4T1-BirAPromoterT7Available SinceJuly 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
GST-AR-1-566
Plasmid#104195PurposeExpresses human AR-1-566 from bacterial expression vector pGEX-5X-1 as GST fusionDepositorAvailable SinceJan. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
Human USP30 (64-502, construct 13, MG-26-82)
Plasmid#110746PurposeBacterial expression of human USP30 (construct 13)DepositorInsertUSP30 (USP30 Human)
TagsN-His6-3C-GST-3CExpressionBacterialMutationF348D, M350S, I353E + insertion deletion: 64-178;…PromoterT7Available SinceJune 14, 2018AvailabilityAcademic Institutions and Nonprofits only