31,059 results
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Plasmid#109289PurposeExpresses high levels of protein in mycobacteriaDepositorTypeEmpty backboneExpressionBacterialMutationpSUM-kan-MCS1-gfp was modified to reorient the Ba…Available SinceJune 1, 2018AvailabilityAcademic Institutions and Nonprofits only
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pGT-Ah2
Plasmid#122569PurposeMAGIC donor plasmid (Himar transposon with beta-lactamase/sfGFP payload)DepositorInsertbeta-lactamase/sfGFP
UseSynthetic BiologyExpressionBacterialAvailable SinceMay 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
SNX5-PX (26-170)
Plasmid#119086PurposeBacterial expression of human phox homology (PX) domain, SNX5-PX (26-170)DepositorAvailable SinceApril 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBEN1-SGC
Plasmid#39127PurposeSGC Empty backbone for bacterial expressionDepositorTypeEmpty backboneTagsStrep tag and SET1ExpressionBacterialAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
MAT2AA-c001
Plasmid#53648Purposebacterial expression plasmidDepositorInsertMAT2AA
TagsHis6-TEVExpressionBacterialMutationBacterial expression for structure determination;…Available SinceJune 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRSET his-mRFP
Plasmid#113553PurposeFor bacterial expression and purification of mRFPDepositorInserthis-mRFP
ExpressionBacterialAvailable SinceJuly 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
AD-003
Plasmid#25502PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceAug. 23, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET28a-baPrs-hdNadV
Plasmid#91950PurposepET28a-baPrs-hdNadV is a bicistronic vector designed for the simultaneous expression of PRS from Bacillus amyloliquefaciens and NadV from Haemophilus ducreyiDepositorInsertsPutative Nicotinamide Phosphoribosyl Transferase (nadV Haemophilus ducreyi, strain: ATCC 27722)
Phosphoribosyl Pyrophosphate Synthetases
ExpressionBacterialMutationchanged Leucine 135 to IsoleucinePromoterT7Available SinceSept. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
MBP-MEK1 S222TAG
Plasmid#68302PurposeN-terminal MBP tagged human MEK1 gene S222TAG for bacterial expressionDepositorAvailable SinceSept. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBJG1 T7-8xHis-OGT 5N5A
Plasmid#154280PurposeBacterial expression of full length human OGT with 5N5A TPR mutations (inefficient substrate binding). N-terminal T7_leader-8xHis tag followed by HRV3C protease site.DepositorInsertO-GlcNAc Transferase (OGT Human)
Tags8x His, HRV3C protease site, and T7 leaderExpressionBacterialMutationN322A, N356A, N390A, N424A, N458A mutations; bact…Available SinceSept. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMAK463
Plasmid#140701PurposeNBαMs-IgKDepositorInsertNBαMs-IgK
ExpressionBacterialAvailable SinceJune 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
Intersectin I SH3 A domain (human)
Plasmid#47413DepositorInsertIntersectin SH3 domain A (ITSN1 Human)
TagsGSTExpressionBacterialMutationonly SH3 domain APromotertacAvailable SinceJuly 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pJ2044
Plasmid#140676PurposeNOT gate with sg-1DepositorInsertNOT gate with sg-1
UseSynthetic BiologyExpressionBacterialAvailable SinceJuly 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
HSP90B1
Plasmid#39076PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMay 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGEX CnA 2-347
Plasmid#13251DepositorInsertCnA (PPP3CA Human)
TagsGSTExpressionBacterialMutationAmino acids 2-347 (truncated before B binding hel…Available SinceOct. 3, 2006AvailabilityAcademic Institutions and Nonprofits only -
pEE077
Plasmid#176834PurposeEncodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expressionDepositorInsert716
Tags6xHisExpressionBacterialPromoterT7Available SinceNov. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPlacPbuCas13b-gRNA-T-MS2
Plasmid#184839PurposeExpression of a single-spacer CRISPR array with spacer #1 targeting the MS2 phage genome and expression of PbuCas13b in bacteria.DepositorInsertsingle-spacer CRISPR array with spacer #1 targeting the MS2 phage genome, PbuCas13b nuclease
UseCRISPR and Synthetic BiologyExpressionBacterialPromoterJ23119 and lac promoterAvailable SinceOct. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pReporter_2
Plasmid#60563PurposeA reporter plasmid containing the attB and attP sites flanking a green fluorescent protein reporter gene (gfp).DepositorInsertReporter_2
ExpressionBacterialPromoterBBa_J23119Available SinceMarch 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pNIC-TolB-NHSTIIT-GG
Plasmid#215792PurposeCloning of target gene with N terminal TolB SRP pathway signal peptide N terminal His6 Twin Strep tag and TEV cleavageDepositorTypeEmpty backboneExpressionBacterialAvailable SinceApril 25, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
HOXC9 (murine) HIS-tag pET
Plasmid#8559DepositorAvailable SinceMay 30, 2006AvailabilityAcademic Institutions and Nonprofits only -
HARS_pNIC-Bio3
Plasmid#153050PurposeExpression construct for producing Avi tagged histidyl tRNA synthetaseDepositorAvailable SinceJune 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
GMPSA
Plasmid#42402PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBPHLWL-GAL4DBD
Plasmid#26256DepositorInsertZIP- Gal4DBD (GAL4 Budding Yeast)
UseDrosophila transgenesisExpressionBacterial and InsectAvailable SinceFeb. 2, 2011AvailabilityAcademic Institutions and Nonprofits only -
pNVLTv2-pvdIJD-Ptrc-base
Plasmid#169243PurposeSuicide vector for integrating expression cassette in P. putida (IPTG induction)DepositorInsertlacI
ExpressionBacterialPromoterlacIqAvailable SinceJune 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
B6-2
Plasmid#111486PurposePro-tRNA synthetaseDepositorInsertPro-tRNA synthetase
Tags6xHisExpressionBacterialAvailable SinceJune 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET-22b(+)-HpENR
Plasmid#12651DepositorInsertnoyl-acyl carrier protein reductase (fabI Helicobacter pylori)
ExpressionBacterialAvailable SinceJan. 5, 2007AvailabilityAcademic Institutions and Nonprofits only -
pGEX Lyn-SH2
Plasmid#46452DepositorInsertLyn (LYN Human)
TagsGST and PreScissionExpressionBacterialMutationcontains amino acids 106-213PromoterTacAvailable SinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
ALFY-FYVE 1G
Plasmid#197045PurposeExpression plasmid for the FYVE domain from ALFY for recombinant expression in E. coli.DepositorAvailable SinceDec. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pXD71Cas10.1RT1-Pct5.1-crRNA(dadR)-RT(ΔdadR)
Plasmid#191635PurposeE. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5.1-crRNA(dadR-targeting spacer) dadR-deleting repair template, used for dadR deletionDepositorInsertCymR
UseCRISPRExpressionBacterialAvailable SinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pXD70Tet(DadR)-DadR
Plasmid#191636PurposeE. coli - Eggerthella lenta shuttle plasmid (TetR), dadR complementationDepositorInsertDadR
ExpressionBacterialAvailable SinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
ZFYVE9C-c009
Plasmid#53618Purposebacterial expression plasmidDepositorInsertZFYVE9C
TagsHis-6-TEVExpressionBacterialMutationBacterial expression for structure determination;…PromoterT7Available SinceJune 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFPcR5
Plasmid#149484PurposeBroad-Host range expression vector, RSF1010 origin, PcaU(AM) repressor, P3B5 promoter, mRFP, Gentamicin Resistance Marker.DepositorInsertPcaU(AM)-mRFP
UseSynthetic BiologyExpressionBacterialPromoterP3B5BAvailable SinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBLO5.01
Plasmid#121418PurposepUC19_BsaI-dCas9-BsaIDepositorInsertdcas9
ExpressionBacterialPromoterlacAvailable SinceMarch 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFP.R371
Plasmid#138241PurposeExpresses the split mCherry reporter interrupted by the MP-M-DnaB artificially mini-intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialPromoterP(araBAD) and iPCAvailable SinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRF-ScbR
Plasmid#49370DepositorInsertScbR repressor
UseSynthetic BiologyExpressionBacterialAvailable SinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_UBASH3B-1/1
Plasmid#91462PurposeProtein expression and purification of human SH3 domain construct UBASH3B-1/1DepositorInsertUBASH3B-1/1 (UBASH3B Human)
ExpressionBacterialAvailable SinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTorPE-B-GECO1
Plasmid#32469DepositorInsertB-GECO1.0
UseLab constructedTags6 His tag and Modified TorA MNNNDLFQASRRRFLAQLG[G…ExpressionBacterialMutationGCaMP3 S40P/L60P/D86G/K119I/L173Q/T223S/Y224H/D30…Available SinceOct. 14, 2011AvailabilityAcademic Institutions and Nonprofits only -
pHisIHFαIHFβ
Plasmid#149384PurposeExpression of P. aeruginosa PA14, 6xHis-HRV3C-IHFalpha and IHFbetaDepositorInsertsPseudomonas aeruginosa PA14 6xHis-tagged IHFalpha
Pseudomonas aeruginosa PA14 IHFbeta
Tags6xHis tag and HRV3C cleavage siteExpressionBacterialPromoterT7Available SinceAug. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pStrepIHFb
Plasmid#149385PurposeExpression of P. aeruginosa PA14, StrepII-HRV3C-IHFbetaDepositorInsertPseudomonas aeruginosa PA14 StrepII-tagged IHFbeta
TagsHRV3C cleavage site and StrepII-tag withExpressionBacterialPromoterT7Available SinceAug. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHH0103 FNBP3 WW domain #1
Plasmid#104233PurposeBacterial expression of WW domain #1 from FNBP3DepositorInsertFNBP3 WW domain #1 (PRPF40A Human)
TagsGSTExpressionBacterialMutationcodon optimized for expression in bacteriaPromotertacAvailable SinceMarch 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-N-Myc
Plasmid#119757PurposeAllows cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag, and a retained N-terminal Myc tag.DepositorTypeEmpty backboneTagsGST and MycExpressionBacterialPromotertac promoterAvailable SinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
Col1A-5'probe
Plasmid#20732DepositorInsertCol1A-5'probe (Col1a1 Mouse)
ExpressionBacterialAvailable SinceApril 24, 2009AvailabilityAcademic Institutions and Nonprofits only -
pGEX Hck-SH2
Plasmid#46445DepositorInsertHck (HCK Human)
TagsGST and PreScissionExpressionBacterialMutationcontains amino acids 134-254PromoterTacAvailable SinceJuly 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
Pet15b-Internalization Control
Plasmid#186553PurposeNegative Control mediating endosomal localizationDepositorInsert10R-8His-EGFP
Tags8xHisExpressionBacterialPromoterT7Available SinceOct. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
ShadowG-SUMO1
Plasmid#175239PurposeBacterial expression and purification, conjugatable SUMO for SUMOylationDepositorAvailable SinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
LB1366 (Bcl-2 promoter with MHS1234)
Plasmid#15388DepositorInsertB-cell lymphoma/leukemia-2 (BCL2 Human)
UseLuciferaseTagsLuciferaseExpressionBacterial and MammalianMutationBcl-2 full promoter with IgH full enhancer MHS1234Available SinceAug. 1, 2007AvailabilityAcademic Institutions and Nonprofits only -
pET His6 Sumo TEV cloning vector with BioBrick polycistronic restriction sites (9S)
Plasmid#48291DepositorTypeEmpty backboneTagsHis6 and SumoExpressionBacterialAvailable SinceSept. 24, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSEVAT7Z1A
Plasmid#58300PurposeGeneGuard vector cassette: thyA, ColE2 COR, Zeta toxin; with ampR as cargo.DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialAvailable SinceAug. 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBEST-p15a-OR2-OR1-Pr-UTR1-sigma28-T500
Plasmid#45779DepositorInsertsigma factor 28
UseSynthetic BiologyExpressionBacterialPromoterOR2-OR1-Pr (bacteriophage Lambda with one mutatio…Available SinceJuly 19, 2013AvailabilityAcademic Institutions and Nonprofits only -
pC236
Plasmid#65951PurposeMedium Repressor plasmid for two-strain systemDepositorInsertCinI
TagsLAA taggedExpressionBacterialPromoterPrhl/lac-mAvailable SinceAug. 13, 2015AvailabilityAcademic Institutions and Nonprofits only