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Plasmid#59948PurposePT7 GFP reporterDepositorInsertsfGFP
UseTagsExpressionBacterialMutationN/APromoterPT7Available sinceOct. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBTB-1
Plasmid#22817DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 22, 2010AvailabilityAcademic Institutions and Nonprofits only -
pET28a-hDcp2 E147Q, E148Q
Plasmid#72215Purposebacterial expression of His-tagged human Dcp2 Q147/8 mutant (disrupts the decapping function of Dcp2)DepositorInsertDcp2 (DCP2 Human)
UseTagsHisExpressionBacterialMutationE147Q, E148QPromoterT7Available sinceDec. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pOPS0384
Plasmid#133234PurposeReporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.DepositorInsertconstructed with NoP (pOGG113), mCherry (EC15071) and T-pharma (pOGG003)
UseTagsExpressionBacterialMutationDomesticated for Golden Gate cloningPromoterAvailable sinceApril 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
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T7pr-His6-MBP-TEV-dCas9-BirA*
Plasmid#159994PurposeBacterial expression of dCas9-BirA*DepositorInsertdCas9-BirA*
UseTags6X-His, MBP, 3X-FLAG, NLSExpressionBacterialMutationD10A, H840APromoterT7Available sinceOct. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBS Rax (CC#5)
Plasmid#15138DepositorInsertRax (Rax Mouse)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pReporter_7
Plasmid#60567PurposeA reporter plasmid containing the attB and attP sites flanking a green fluorescent protein reporter gene (gfp).DepositorInsertReporter_7
UseTagsExpressionBacterialMutationPromoterBBa_J23119Available sinceMarch 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pUC18PV-mini-Tn7T
Plasmid#64956PurposepUC18-mini-Tn7T with unique PvuII site in pUC18 DNADepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 6, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pHH0103_VAV2-2/2
Plasmid#91506PurposeProtein expression and purification of human SH3 domain construct VAV2-2/2DepositorInsertVAV2-2/2 (VAV2 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_NOXO1-1/2
Plasmid#91499PurposeProtein expression and purification of human SH3 domain construct NOXO1-1/2DepositorInsertNOXO1-1/2 (NOXO1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pC210
Plasmid#65950PurposeWeak Repressor plasmid for two-strain systemDepositorInsertCinI
UseTagsLAA taggedExpressionBacterialMutationPromoterPrhl/lac-wAvailable sinceAug. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
Pk-SOD1
Plasmid#25574PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertPk-SOD1
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceSept. 10, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
pRSETA_ionRD
Plasmid#172924PurposeIonic strength FRET sensor with mCerulean3 and mCitrine for E. coli expression.DepositorInsertIonicStrengthSensorRD
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceAug. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGST-GstZ1
Plasmid#163090PurposeExpressing mouse Gstz1 in bacterial cellsDepositorInsertsGstZ1
GstZ1
UseTagsGSTExpressionBacterialMutationPromoterT7Available sinceJan. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBT3019
Plasmid#122541PurposeExpression of a C. elegan codon optimized florescent protein (CemOrange2) fused to a peroxisome target peptide (SKL) under the intestinal specific promoter nhx-2DepositorInsertPnhx-2_CemOrange2::SKL
UseTagsSKLExpressionBacterialMutationPromoterPnhx-2Available sinceMarch 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pET-30a(+)-Syk-tSH2-PM
Plasmid#111271Purposeexpress murine Syk tandem SH2 domains (Ser 8 to Gln 264), with Y130E mutation to mimic phosphorylation, in E.coli strain Rosetta 2 (DE3)DepositorInsertmurine Syk tandem SH2 domains (Ser 8 to Gln 264), with Y130E mutation to mimic phosphorylation (Syk Mouse)
UseTagsExpressionBacterialMutationY130E mutation to mimic phosphorylationPromoterT7 promoterAvailable sinceJuly 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET15b-12x
Plasmid#61699PurposeExpresses PTDH mutant Opt12xDepositorInsertOpt12x
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceMarch 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBS-beta1,4 GT-1
Plasmid#11872DepositorInsertbeta 1,4 galactosyltransferase (B4GALT1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 12, 2006AvailabilityAcademic Institutions and Nonprofits only -
pSKA397
Plasmid#80380Purposemini-Tn7 delivery plasmid with metE under the cpcG2Δ59 promoter.DepositorInsertscat
metE
UseSynthetic Biology; Mini-tn7 delivery vectorTagsExpressionBacterialMutationPromotercat promoter and cpcG2Δ59Available sinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pJ2042.2
Plasmid#140670Purpose3-color stripeDepositorInsert3-color stripe
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceSept. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
B2-3
Plasmid#111470PurposeLys-tRNA synthetaseDepositorInsertLys-tRNA synthetase
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceJuly 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMS3: pHelper(AvCAST)_AvPSP1_ΔTnsD
Plasmid#168135PurposeInducible expression of AvCAST proteins (except TnsD) with crRNA targeting AvPSP1.DepositorInsertsAvCAST minimal CRISPR array (with spacer for AvPSP1)
AvCAST Cascade proteins (Cas6, Cas8, Cas7 and Cas5)
AvCAST Tns proteins (TnsA, TnsB, TnsC and TniQ)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET16b-Trx C35S
Plasmid#36947DepositorInsertThioredoxin-1 C35S mutation (TXN Human)
UseTagsHis tagExpressionBacterialMutationcys 35 to serinePromoterT7Available sinceMay 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
SNAP-MIDAS
Plasmid#171604PurposeExpresses the SNAP-tagged MIDAS domain of S. pombe Mdn1 (a.a. 4381-4717) in bacteria.DepositorInsertMIDAS domain of S. pombe Mdn1 (a.a. 4381-4717)
UseTagsHis6 tag and SNAP tagExpressionBacterialMutationPromoterAvailable sinceJuly 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAT-9208
Plasmid#124221PurposeCloning plasmid for a self-targeting gRNA libraryDepositorInsertself-targeting gRNA
UseCRISPRTagsExpressionBacterialMutationPromoterJ23119Available sinceDec. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
PAK7_HUMAN_D0
Plasmid#79706PurposeThis plasmid encodes the kinase domain of PAK7. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorInsertPAK7 (PAK5 Human)
UseTagsHis10-TEVExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGT-Ah6
Plasmid#122573PurposeMAGIC donor plasmid (Himar transposon with catP/sfGFP payload)DepositorInsertcatP/sfGFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceApril 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBSPPc-Gm
Plasmid#116919PurposeGene constitutive expression from a Pc promoterDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterPc promoter from Delftia acidovorans C17Available sinceOct. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a-ybbr-HIS-ELP-ddFLN4-I27-XMod-Doc (wild type)
Plasmid#153442PurposeE. coli expression of Rc. XDocB wild-type construct containing ybbr tag, ELP linker and ddFLN4 and I27 fingerprint domains. This construct was designed for AFM measurements.DepositorInsertRc.XDocB
UseTags3xELP linker, 6xHis, Titin I27, ddFLN4 fingerprin…ExpressionBacterialMutationPromoterT7 promoterAvailable sinceSept. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T-1-p19-T7-PLK1
Plasmid#46312DepositorInsertsp19
PLK1 DNA-1
linker
PLK1 DNA-2
UsePro-sirnaTags6XHis and GSTExpressionBacterialMutationPromoterAvailable sinceJan. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_TNK1-1/1-SV1/3
Plasmid#91548PurposeProtein expression and purification of human SH3 domain construct TNK1-1/1-SV1/3DepositorInsertTNK1-1/1-SV1/3 (TNK1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_VAV3-1/2
Plasmid#91528PurposeProtein expression and purification of human SH3 domain construct VAV3-1/2DepositorInsertVAV3-1/2 (VAV3 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_SORBS3-1/3
Plasmid#91534PurposeProtein expression and purification of human SH3 domain construct SORBS3-1/3DepositorInsertSORBS3-1/3 (SORBS3 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_ASAP2-1/1
Plasmid#91504PurposeProtein expression and purification of human SH3 domain construct ASAP2-1/1DepositorInsertASAP2-1/1 (ASAP2 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_ARHGAP4-1/1
Plasmid#91518PurposeProtein expression and purification of human SH3 domain construct ARHGAP4-1/1DepositorInsertARHGAP4-1/1 (ARHGAP4 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_VAV3-2/2
Plasmid#91524PurposeProtein expression and purification of human SH3 domain construct VAV3-2/2DepositorInsertVAV3-2/2 (VAV3 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_SH3YL1-1/1
Plasmid#91485PurposeProtein expression and purification of human SH3 domain construct SH3YL1-1/1DepositorInsertSH3YL1-1/1 (SH3YL1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_ARHGAP9-1/1
Plasmid#91442PurposeProtein expression and purification of human SH3 domain construct ARHGAP9-1/1DepositorInsertARHGAP9-1/1 (ARHGAP9 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_NEBL-1/1
Plasmid#91383PurposeProtein expression and purification of human SH3 domain construct NEBL-1/1DepositorInsertNEBL-1/1 (NEBL Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_NPHP1-1/1
Plasmid#91358PurposeProtein expression and purification of human SH3 domain construct NPHP1-1/1DepositorInsertNPHP1-1/1 (NPHP1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_PACSIN1-1/1
Plasmid#91372PurposeProtein expression and purification of human SH3 domain construct PACSIN1-1/1DepositorInsertPACSIN1-1/1 (PACSIN1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_SH3D21-1/3
Plasmid#91296PurposeProtein expression and purification of human SH3 domain construct SH3D21-1/3DepositorInsertSH3D21-1/3 (SH3D21 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_MAP3K9-1/2
Plasmid#91313PurposeProtein expression and purification of human SH3 domain construct MAP3K9-1/2DepositorInsertMAP3K9-1/2 (MAP3K9 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_ARHGAP12-1/1
Plasmid#91282PurposeProtein expression and purification of human SH3 domain construct ARHGAP12-1/1DepositorInsertARHGAP12-1/1 (ARHGAP12 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_MYO15A-2/2
Plasmid#91291PurposeProtein expression and purification of human SH3 domain construct MYO15A-2/2DepositorInsertMYO15A-2/2 (MYO15A Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only