32,656 results
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Plasmid#245311PurposeFragmid fragment: (N' terminus) TadA-CDdDepositorInsertTadA-CDd
ExpressionBacterialMutationNoneAvailable SinceOct. 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pXTK-BB2 [Backbone_RK2-KanR-TetR_PartTypeBB]
Plasmid#229388PurposeXanthoMoClo Golden Gate Backbone Plasmid, contains Backbone_RK2-KanR-TetR_PartTypeBB and an sfGFP dropoutDepositorTypeEmpty backboneExpressionBacterialAvailable SinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pXTK084 [CDS_mRFP_PartType9]
Plasmid#229321PurposeXanthoMoClo Golden Gate Part Plasmid for cloning, contains CDS_mRFP_PartType9DepositorInsertmRFP
ExpressionBacterialAvailable SinceMarch 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
HisMS2_VLP_FluB
Plasmid#175956PurposeGeneration of MS2 Virus-Like Particles packaged with the sequence for the Influenza B NSP 1 protein [B/Colorado/06/2017]DepositorInsertNEP gene
ExpressionBacterialPromoterT7Available SinceSept. 19, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBP-P_luxR-PLuxB
Plasmid#204073PurposeType 0 promoter partDepositorInsertluxR-PLuxB repressor and PLuxB 3OC6-AHL-inducible promoter
ExpressionBacterialMutationNoneAvailable SinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBP-P_rhaS-PRhaB
Plasmid#204076PurposeType 0 promoter partDepositorInsertrhaS activator and PRhaB rhamnose-inducible promoter
ExpressionBacterialMutationNoneAvailable SinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
eSrtA-MT3
Plasmid#200305PurposeBacterial expression plasmids for self cleavable sortase mediated purification of recombinant MT3. Contains His-tag, SUMO-tag, LPETG sortase recognition motif and eSrtADepositorAvailable SinceJuly 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSIBR-Int4-Cas9-NT
Plasmid#235166PurposeExpresses the SIBR-Int4-Cas9 system and a NT sgRNADepositorInsertSIBR-Int4-Cas9
ExpressionBacterialAvailable SinceAug. 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
sgRNA_expression_vector
Plasmid#210212PurposeAn empty gRNA expression vector to clone U6 promoter driven sgRNAs with gRNA scaffold and with co-expression of DsRed. sgRNA can be cloned by Golden Gate Assembly or restriction digestion using BbsI.DepositorTypeEmpty backboneExpressionBacterial and MammalianPromoterU6Available SinceDec. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSH624-ssr
Plasmid#199241PurposeExpresses the gene codying for the Ssr recombinase from Pseudomonas putida DOT-T1EDepositorInsertsingle-stranded DNA recombineering
ExpressionBacterialPromoterlacIq-PtrcAvailable SinceOct. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRSET-QUE2m
Plasmid#129350PurposeATP measurementDepositorInsertQUEEN-2m
ExpressionBacterialAvailable SinceOct. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET29b-H6_Streptavidin_sfGFP
Plasmid#124296PurposeHis6-tagged Streptavidin GFP fusion proteinDepositorInsertHis6 tag - TEV protease site - T7 tag - Streptavidin (15-159) - superfolder GFP
TagsHis6 and T7 tagsExpressionBacterialAvailable SinceApril 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1‐hsRNASEH2BCA
Plasmid#108692PurposeFor expression in E coli of N-terminally GST-tagged human RNASEH2B and untagged RNASEH2C and A, allowing affinity purification of the RNase H2 trimeric enzymeDepositorTagsGSTExpressionBacterialMutationShine Dalgarno sequence upstream of start codon a…PromotertacAvailable SinceApril 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSH424-ssr
Plasmid#198026PurposeExpresses the gene codying for the Ssr recombinase from Pseudomonas putida DOT-T1EDepositorInsertT0 terminator - Sm resistance cassette
ExpressionBacterialPromoterSmR promoterAvailable SinceOct. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET-28b-RfxCas13d-His
Plasmid#141322PurposePlasmid for bacterial expression and purification of RfxCas13d proteinDepositorInsertRfx-Cas13d
UseCRISPRTags6xHisExpressionBacterialPromoterT7Available SinceAug. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28a-mCherry-CNA35
Plasmid#61607Purposebacterial expression of N-terminal fusion protein of CNA35 with mCherryDepositorInsertmCherry and CNA35
TagsHis tagExpressionBacterialPromoterT7Available SinceMarch 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAM5409
Plasmid#132662PurposeRSF1010-based broad host-range plasmid with RK2 bom site expressing YFP and Sp/Sm resistanceDepositorInsertsaadA
YFP
ExpressionBacterialPromoterPaadA and PconIIAvailable SinceNov. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTECH-chPylRS(IPYE)
Plasmid#99222PurposeExpression plasmid encoding chPylRS(IPYE) and pylTDepositorInsertPlpp chPyl(IPYE)RS; PproK pylT
ExpressionBacterialMutationContains activating mutations V31I, T56P, H62Y, A…PromoterPlpp and PproKAvailable SinceOct. 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pACYC-Skp-GroEL/ES
Plasmid#83922PurposeCo-expresses cytoplasmic copies of the Skp and Gro EL/ES protein folding chaperones.DepositorInsertsSkp
Gro EL/ES
ExpressionBacterialPromoterT7Available SinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSLQ14101 aeBlue
Plasmid#239453Purposeexpress aeBlue chromoproteinDepositorInsertaeBlue
UseCRISPR; Cell-free system using bacterial extractExpressionBacterialPromoterJ23119Available SinceJune 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
pQE-80L MBP-SspB Nano
Plasmid#60409PurposeE. coli expression of SspB NanoDepositorInsertSspB nano
TagsHis-MBP-TEVExpressionBacterialMutationY11K and A15EAvailable SinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pProEx-HTa-SOD1 WT
Plasmid#191849Purposeexpress wild-type SOD1 in bacterial cellsDepositorInsertSOD1 (SOD1 Human)
ExpressionBacterialAvailable SinceJan. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSIBR2.0-818-Cas12a-NT
Plasmid#235168PurposeExpresses the SIBR2.0-818-Cas12a system and a NT crRNADepositorInsertSIBR2.0-818-Cas12a
ExpressionBacterialAvailable SinceJuly 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
SPLICSFAST-LDh-MT-P2A
Plasmid#214896PurposeExpresses equimolar Lipid Droplet- and Mitochondria- targeted split-FAST fragments for Short-range LD-MT Contact sitesDepositorInsertSPLICSFAST-LDh-MT-P2A
TagsnoneExpressionBacterial and MammalianAvailable SinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTECH-chAcK3RS(IPYE)
Plasmid#104069PurposeExpression plasmid encoding chAcK3RS(IPYE) and pylTDepositorInsertPlpp chAcK3RS(IPYE); PproK pyl
ExpressionBacterialMutationContains activating mutations V31I, T56P, H62Y, A…PromoterPlpp and PproKAvailable SinceDec. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
JC Mad-1
Plasmid#25626DepositorInsertJC Virus Mad-1
ExpressionBacterialAvailable SinceOct. 20, 2011AvailabilityAcademic Institutions and Nonprofits only -
pREDusk-MCS
Plasmid#188970PurposeFor red light-repressed bacterial expression of gene of interestDepositorTypeEmpty backboneExpressionBacterialAvailable SinceNov. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pON.mCherry
Plasmid#84821PurposeConstitutive Expression plasmid with mCherry fluorescent proteinDepositorInsertmCherry
ExpressionBacterialPromoterTac PromoterAvailable SinceFeb. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDXinit
Plasmid#110101PurposeE.coli entry and expression vector for FX cloning system, N-terminal pelB signal sequence and C-terminal fusion to PIII for phage display using M13 phagesDepositorTypeEmpty backboneExpressionBacterialAvailable SinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBAD-ColH-HylB
Plasmid#248631Purposeexpresses Myc-tagged ColH and His-tagged HylB as two separate proteins in E. coliDepositorInsertMyc-tagged bacterial collagenase, ColH, and His-tagged hyaluronidase, HylB
TagsHis and MycExpressionBacterialPromoteraraBADAvailable SinceDec. 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaM-4
Plasmid#172770PurposeBacterial expression of anti-mCherry nanobody LaM-4, with pelB leader and C-term free cysteine and 6xHIS tag.DepositorInsertLaM-4
Tags6xHIS and pelB leader for periplasmic secretionExpressionBacterialPromoterT7Available SinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBR322-sgRNA-pvc18-20-Pdp1_NTD-Cre
Plasmid#243748PurposePVC cargo/regulatory region - deleted toxin cargos (Pdp1/Pnf) - vegfa sgRNA - Cre loaded into the PVC tube via Pdp1 (NTD)DepositorInsertvegfa sgRNA-pvc18-20-Pdp1_NTD-Cre
ExpressionBacterialAvailable SinceSept. 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pNCS-mRuby3
Plasmid#74234PurposeConstitutive expression of mRuby3 in bacteriaDepositorInsertmRuby3
Tags6xHisExpressionBacterialPromoterT7Available SinceMay 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTD103luxI_sfGFP
Plasmid#48885PurposePositive feedback plasmid from oscillatorDepositorInsertLuxI, sfGFP, and LuxR
UseSynthetic BiologyTagsssrA tag (AANDENYALAA)ExpressionBacterialPromoterpLuxAvailable SinceFeb. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET296-YcpLac111 CYC1p-MCP-NLS-2xyeGFP
Plasmid#104394PurposeYeast MCP-GFP expressionDepositorInsertMCP-NLS-2xyeGFP
ExpressionBacterial and YeastMutationMCP fused to NLS(SV40) and 2x yeast codon optimiz…PromoterCYC1Available SinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
p11d-tRPA(123)
Plasmid#102613PurposeExpression of human RPA in E. coliDepositorAvailable SinceNov. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAJF1131
Plasmid#252906PurposeT7-pelB-NBALFA-LE-6his amp (for purification of his-tagged NBALFA from E. coli)DepositorInsertNBALFA
TagsHis Tag and pelB leaderExpressionBacterialPromoterT7Available SinceMarch 30, 2026AvailabilityAcademic Institutions and Nonprofits only -
pTW335c3
Plasmid#237322Purposeexpresses A. thaliana RbcL RbcS-6xHisDepositorInsertsTags6xHis and NoneExpressionBacterialAvailable SinceMay 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pDSG289
Plasmid#115608Purposesurface-displayed Nb2, used in composability experiments; low copyDepositorInsertregulated adhesin construct
ExpressionBacterialAvailable SinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDSG291
Plasmid#115605Purposesurface-displayed Null, used in composability experiments; low copyDepositorInsertregulated adhesin construct
ExpressionBacterialAvailable SinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
Lac-I-SceI-Tet
Plasmid#17655DepositorInsertlac I-SceI tet
ExpressionBacterialAvailable SinceMay 30, 2008AvailabilityAcademic Institutions and Nonprofits only -
pDSG287
Plasmid#115606Purposesurface-displayed Ag2, used in composability experiments; low copyDepositorInsertregulated adhesin construct
ExpressionBacterialAvailable SinceOct. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET11a-SUMO1
Plasmid#53138PurposeE.coli expression of untagged mature human SUMO1 (amino acids 1-97)DepositorAvailable SinceSept. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pUltra-sY
Plasmid#82417PurposeExpresses Mj aaRS for sulfotyrosine and Mj tRNA for decoding UAG codonsDepositorInsertMethanococcus jannaschii aaRS for sulfotyrosine
ExpressionBacterialMutationTyr32Leu, Leu65Pro, Asp158Gly, Ile159Cys, Leu162L…PromotertacIAvailable SinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEvol-pAcFRS.2.t1
Plasmid#73544PurposetRNA synthetase/tRNA pair for the in vivo incorporation of several non-standard amino acids, into proteins in E coli in response to the amber codon, TAGDepositorInsertspAcFRS.2.t1
pAcFRS.2.t1
ExpressionBacterialAvailable SinceMarch 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDH42(CUG/A)
Plasmid#154132PurposeExpresses engineered (23S-5S phusion) rRNAs operon in bacterial cellsDepositorInsertengineered bacterial rRNAs operon under control of pL promoter
UseSynthetic BiologyExpressionBacterialAvailable SinceOct. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
SuperNova/pRSETB
Plasmid#53234Purposemonomeric photosensitizing fluorescent protein for chromophore-assisted light inactivationDepositorInsertmonomeric photosensitizing fluorescent protein for chromophore-assisted light inactivation
Tags6xHis, T7 tag, and Xpress tagExpressionBacterialAvailable SinceJune 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAWP78-PVCpnf_pvc13-Ad5_HUHe-Pvc10-Cas9
Plasmid#243744PurposePVC structural operon - tail fiber (Pvc13) retargeted with Ad5 knob - spike tip (Pvc10) loaded with HUHe on NTD and Cas9 on CTDDepositorInsertpvc13-Ad5_HUHe-Pvc10-Cas9
ExpressionBacterialAvailable SinceSept. 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pF711-pET28a-Hs-delta25-MCAD-NHis wt
Plasmid#85112Purposeexpression of recombinant human mature (delta25) MCAD wt with N-terminal 6xHis-tagDepositorAvailable SinceNov. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_FRS2_IRS
Plasmid#109815PurposeProtein expression and purification of FRS2_IRSDepositorInsertFRS2_IRS (FRS2 Human)
ExpressionBacterialAvailable SinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only