32,161 results
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DVL2
Plasmid#38876PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTn7xTS
Plasmid#117389PurposeTn7 tagging vector with temperature-sensitive origin of replicationDepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialAvailable SinceDec. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAB078d9
Plasmid#79207PurposeDirected Evolution of Protein-Protein InteractionsDepositorInsertPlacZ-opt (OR1+2+3) luxAB; Ppro1 434cI-SH2ABL1
ExpressionBacterialPromoterPlacZ-opt (OR1+2+3); Ppro1Available SinceAug. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAP974-1
Plasmid#99497PurposeTEV::linker::tagRFP::3XFlagDepositorInsertTEV::linker::tagRFP::3XFlag
ExpressionBacterialAvailable SinceOct. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCAM17
Plasmid#86473PurposeExpresses p12 and p9DepositorInsertp12, p9
UseSynthetic BiologyTagsnoneExpressionBacterialPromoterpBADAvailable SinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
BRPF1B-c001
Plasmid#53620Purposebacterial expression plasmidDepositorInsertBRPF1B
TagsHis-6-TEVExpressionBacterialMutationBacterial expression for structure determination;…PromoterT7Available SinceJune 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMVV098
Plasmid#215304PurposeExpresses sfGFP under induction with the DAPG Marionette Cassette (PhIFAM + PPhIF-sfGFP) with a BBR1 backbone.DepositorInsertsfGFP
UseSynthetic BiologyTagsNoneExpressionBacterialPromoterPPhIFAvailable SinceJuly 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRU1105
Plasmid#14469DepositorInsertDsRedT3
ExpressionBacterialAvailable SinceApril 23, 2007AvailabilityAcademic Institutions and Nonprofits only -
pdUA-lacIN
Plasmid#141079PurposePlasmid vector for testing DNA binding proteins by the I-Block assay.DepositorTypeEmpty backboneUseVector for the i-block assay designed to study dn…ExpressionBacterialPromoterEscherichia coli lacIN promoterAvailable SinceSept. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pM
Plasmid#64179Purposeharbours marker gene MET17DepositorTypeEmpty backboneExpressionBacterial and YeastAvailable SinceAug. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCas9-J23109
Plasmid#113150PurposePlasmid constitutively expressing Cas9 proteinDepositorInsertCas9
ExpressionBacterialAvailable SinceDec. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEB2-mCherry-L
Plasmid#104002PurposePlasmid encoding mCherry-LDepositorInsertmCherry-L
UseLow copyExpressionBacterialMutationMutations relative to DsRed (MSKGEE DNLA IIKEF… V…PromoterproCAvailable SinceDec. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFPV25.1_dGFPmut3-AAV
Plasmid#187377PurposerpsM promoter driving the expression of destabilized GFPmut3 (containing a AAV C-terminal tail)DepositorInsertdGFPmut3_AAV
TagsAAVExpressionBacterialMutationC-terminal AAV tailAvailable SinceAug. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBluescript-mouse eIF2C2
Plasmid#50354PurposeMouse eIF2C2 cDNA cloneDepositorAvailable SinceJan. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBAV-A
Plasmid#65927PurposeTheophylline Riboswitch A with lacZ geneDepositorInsertlacZ
ExpressionBacterialPromoterT5Available SinceSept. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET22b-BirA-CPDSalI
Plasmid#38258DepositorInsertbirA (birA E. coli)
Tags6xHis and Vibrio cholerae MARTX toxin cysteine pr…ExpressionBacterialMutationcloned from pGEX4T1-BirAPromoterT7Available SinceJuly 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
pET-deSpCas9-VP64-6xHis
Plasmid#92117PurposeExpression of dead/inactive increased fidelity eSpCas9 (1.1)-VP64-6xHis in bacterial cellsDepositorInsertdead/inactive eSpCas9-NLS-3xFLAG-VP64
UseCRISPRTags3xFLAG, 6xHis, NLS, and VP64ExpressionBacterialMutationD10A, H840A, K848A, K1003A, R1060APromoterT7Available SinceOct. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4136461
Plasmid#149538PurposeBacterial expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)DepositorInsertSARS-CoV-2 S (surface glycoprotein) (S Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
Tags6xHIS;Linker_GGSG;MBP;Linker_GGSG;Cleavable TEVExpressionBacterialMutationwtPromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available SinceMay 14, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET24a-VHH-tev-std
Plasmid#109423PurposeBacterial expression of a functionalized anti-GFP (VHH) nanobody with a TEV site. VHH-tev-std is flanked by an N-terminal T7 epitope, and a C-terminal cassette composed of HA, BAP and a His6 epitopeDepositorInsertanti-GFP nanobody fused to a T7, TEV cleavage site, HA, BAP and His6 epitope
TagsBAP, HA, His6, and T7ExpressionBacterialPromoterT7Available SinceJune 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
Chx10 3kb AP (CC#318)
Plasmid#15205DepositorInsertChx10 3kb AP reporter (Vsx2 Mouse)
ExpressionBacterialAvailable SinceJune 8, 2007AvailabilityAcademic Institutions and Nonprofits only -
pGEX2TK-Num1-PH
Plasmid#71369Purposebacterial expression of PH domain for phosphoinositide binding assayDepositorInsertNum1-PH
TagsGSTExpressionBacterialMutationPleckstrin homology (PH) domain; aa2563-2692PromotertacAvailable SinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
DT049A
Plasmid#159410PurposeConstitutive murine mature BDNF expression, controlled under E.coli tet promoterDepositorInsertpr.tet-BDNF (Bdnf, Mouse) (Bdnf Synthetic, Mouse)
UseSynthetic BiologyTagsFLAGExpressionBacterialPromotertet promoterAvailable SinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pdCas9-M-3A2
Plasmid#73435PurposeCRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant 3A2.DepositorInsertRepressor 3A2 (orthogonal T7-lac repressor)
UseCRISPR and Synthetic BiologyExpressionBacterialPromoterConstitutive wild-type S. pyogenes promoterAvailable SinceMarch 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJJS009
Plasmid#188332PurposePCR template for repair templates to tag a gene with a mKate2 and mIAA7 AID degron using homology-directed repair for C. elegans.DepositorInsertmIAA7::mKate2::Linker
ExpressionBacterialAvailable SinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCF525-EF1a-Hygro-P2A-AcrVA4-lenti
Plasmid#115663PurposeExpresses AcrVA4 in human cells, contained in a lentiviral vector.DepositorInsertAcrVA4
UseLentiviralExpressionBacterial and MammalianPromoterEF-1a promoterAvailable SinceMarch 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUC-UAS1B4-Leum
Plasmid#44312DepositorInsertUAS1B4-Leum promoter
UseSynthetic BiologyExpressionBacterialMutationN/APromoterUAS1B4-LeumAvailable SinceJuly 15, 2013AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGEX-4T Syt1 C2AB(140) WT
Plasmid#134695PurposeEncodes cytoplasmic domain of synaptotagmin-1 for bacterial expression and purificationDepositorAvailable SinceNov. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHsSR beta deltaN Flag
Plasmid#13731DepositorInsertSignal recognition particle receptor β subunit (SRPRB Human)
Tags6xHis and FLAGExpressionBacterialMutationexpresses residues 59-271 of Homo sapiens SRβ wit…Available SinceJan. 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
pEB2-TurboRFP
Plasmid#103996PurposePlasmid encoding TurboRFPDepositorInsertTurboRFP
UseLow copyExpressionBacterialMutationMutations relative to eqFP578 (R32G, T68A, L79F, …PromoterproCAvailable SinceDec. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046809
Plasmid#145685PurposeBacterial Expression plasmid for SARS-CoV-2 2'-O-ribose methyltransferaseDepositorInsertSARS-CoV-2 2'-O-ribose methyltransferase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
TagsCleavable TEV;6xHISExpressionBacterialMutationwtPromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available SinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET:GACT:GFP
Plasmid#60734PurposeA modified pET28 driving expression of GFP, biobrick part Bba_E0040. No LacO site downstream of PT7. T7 promoter has base pairs 'GACT' between -11 and -8.DepositorInsertGFP
UseSynthetic BiologyExpressionBacterialAvailable SinceNov. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pES133
Plasmid#127552PurposeVariant of pKD13. Lambda Red recombineering PCR template plasmid carrying an F5-tetA-F5 cassette orthogonal to FRT-KanR-FRT in pKD13DepositorInsertF5-flanked tetracycline resistance cassette
ExpressionBacterialMutationMultiple SNPs in tetA (originally from pBR322) to…Available SinceJuly 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcrRNA.con
Plasmid#61285PurposeConstitutive repeat-only E. coli CRISPR arrayDepositorTypeEmpty backboneUseCRISPRExpressionBacterialPromoterJ23119Available SinceFeb. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
ARF1
Plasmid#28168PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceFeb. 28, 2011AvailabilityIndustry, Academic Institutions, and Nonprofits -
p5084
Plasmid#168179Purposebacteria reporterDepositorInsertPEM7::dsred PmsrA::sfgfp
ExpressionBacterialMutationnoneAvailable SinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBT3037
Plasmid#122544PurposeExpression of a C. elegan codon optimized florescent protein (CemOrange2) fused to a autophagic localized gene that is orthologus to mamalian p62 (sqst-1) under the intestinal specific promoter nhx-2DepositorAvailable SinceMarch 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJPC12-ΔM13-mCherry-P/PM,5C6A-GFP
Plasmid#80910PurposeReporter plasmid (mCherry and GFP)DepositorInsertsmCherry
GFP
ExpressionBacterialPromoterP5C6A and PM,5C6AAvailable SinceDec. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSP72-deltaFlincG
Plasmid#19733DepositorAvailable SinceOct. 27, 2008AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_NR6A1_Hormone-recep
Plasmid#109945PurposeProtein expression and purification of NR6A1_Hormone-recepDepositorInsertNR6A1_Hormone-recep
ExpressionBacterialAvailable SinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only
