32,656 results
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Plasmid#239948PurposeExpression of MFSD6 loop 3 tagged with rabbit IgG Fc2DepositorInsertFc2-MFSD6(L3)
ExpressionBacterial and MammalianAvailable SinceJuly 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBJG1 T7-8xHis-OGT
Plasmid#154271PurposeBacterial expression of full length human OGT. N-terminal T7_leader-8xHis tag followed by HRV3C protease site.DepositorInsertO-GlcNAc Transferase (OGT Human)
Tags8x His, HRV3C protease site, and T7 leaderExpressionBacterialMutationbacterial codon optimizedAvailable SinceSept. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28(a)-p2c-Cas9
Plasmid#242759PurposeExpresses Cas9 nuclease with fused p2c targeting ligand for expression in E. coliDepositorInsertP2C-Cas9
UseCRISPRExpressionBacterialPromoterlacAvailable SinceSept. 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
pTSlb-R756K
Plasmid#60731PurposeExpresses both fragments of T7 RNAP split at position 179. The N-terminal fragment is driven by PLac while the C-terminal fragment is driven by PBAD. C-terminal fragment has the point mutations R756SDepositorInsertsResidues 1-179 of split T7 RNAP
Residues 180-880 of split T7 RNAP
UseSynthetic BiologyExpressionBacterialMutationResidues 1-180 of split T7 RNAP and Residues 180-…Available SinceNov. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
PylRS-AS
Plasmid#137908PurposeFor incorporation of fluorophenylalanine derivativesDepositorInsertPyrrolysyl-tRNA synthetase/pyrrolysyl -tRNA pair
ExpressionBacterialMutationContaining N346A, C348S mutationsPromoterpBADAvailable SinceFeb. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
His-TEV-SNAP-PRC1
Plasmid#167541PurposeFor expression of His6-TEV-SNAP-PRC1 in bacteria.DepositorAvailable SinceSept. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
MZB209
Plasmid#228492PurposeBirA encoded pACYC vector for co-expression with Avi-tagged constructs (Bacterial Expression)DepositorAvailable SinceJan. 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_GNA15_G-alpha
Plasmid#109782PurposeProtein expression and purification of GNA15_G-alphaDepositorInsertGNA15_G-alpha (GNA15 Human)
ExpressionBacterialAvailable SinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSL8215 (dCas12f–σE (sigmaE) system, entry vector)
Plasmid#254272PurposeEncodes E. coli codon-optimized dCas12f and σE (sigmaE) from Flagellimonas taeanensis driven by constitutive promoters, with BsaI-containing restriction sites for guide RNA cloningDepositorInsertsdCas12f
sigma E
UseCRISPRExpressionBacterialPromoterJ23105Available SinceApril 6, 2026AvailabilityAcademic Institutions and Nonprofits only -
pSL7458 (Fta RNA polymerase)
Plasmid#254269PurposeEncodes E. coli codon-optimized rpoA, rpoB, rpoC, and rpoZ from Flagellimonas taeanensis driven by an inducible T7 promoterDepositorInsertsrpoA
rpoB
rpoC
rpoZ
ExpressionBacterialPromoterT7Available SinceApril 2, 2026AvailabilityAcademic Institutions and Nonprofits only -
pET-hisTdT
Plasmid#216674PurposeExpresses his-tagged N-terminus truncated TdT in E. coli hostsDepositorInsertterminal deoxynucleotidyl transferase
Tagshis-tagExpressionBacterialMutation1-138 truncationPromoterT7Available SinceNov. 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET-6xHis-GFP(-7)-neg_tag1
Plasmid#219419PurposeFluorescent protein to reconstruct the mixture that mimics the charge profile of E. coli lysateDepositorInsertGFP(-7)-neg_tag1
TagsDEEEDD and MGHHHHHGGExpressionBacterialPromoterT7Available SinceDec. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-6xHis-GFP(-12)
Plasmid#219420PurposeFluorescent protein to reconstruct the mixture that mimics the charge profile of E. coli lysateDepositorInsertGFP(-12)
TagsMGHHHHHGGExpressionBacterialPromoterT7Available SinceDec. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBAC-lacZ
Plasmid#13422DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseCre/LoxExpressionBacterialAvailable SinceFeb. 8, 2007AvailabilityAcademic Institutions and Nonprofits only -
pAGM22082_sfGFP1-10
Plasmid#153515PurposeE.coli expression plasmid for the production of GFP1-10DepositorInsertsfGFP1-10
ExpressionBacterialAvailable SinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a SpyCatcher-SnoopCatcher
Plasmid#72324PurposeBacterial expression of SpyCatcher linked to SnoopCatcher.DepositorInsertpET28a SpyCatcher-SnoopCatcher
TagsN-terminal His6 tagExpressionBacterialMutation35 amino acids at the N-term of SpyCatcher have b…Available SinceFeb. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
MPRA_minCMV+GFP
Plasmid#204866PurposeVector containing GFP driven by minimal CMV promoter. This cassette can be cloned into MPRA vectors to serve as a reporter gene.DepositorInsertminCMV:GFP
ExpressionBacterial and MammalianPromoterminimal CMVAvailable SinceSept. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
GST-MNase
Plasmid#136291PurposeExpresses Micrococcal nucleaseDepositorInsertMNase
ExpressionBacterialAvailable SinceFeb. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pST44
Plasmid#64007Purposepolycistronic cloning vector for pST44 plasmid suiteDepositorTypeEmpty backboneExpressionBacterialPromoterT7Available SinceNov. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEXP5-NT/pLux eGFP
Plasmid#193624PurposeInducible expression of eGFP by the transcription factor complex LuxR-3OC6 homoserine lactone (HSL).DepositorInserteGFP
Tags6xHisExpressionBacterialPromoterpLuxAvailable SinceJuly 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLacO-ISce1
Plasmid#58505Purposeprovides 256 Lac operator repeats adjacent to the ISce1 restriction siteDepositorInsertLac repeats adjacent to 6 copies of the ISce1 restriction site
ExpressionBacterialPromoterno promoterAvailable SinceOct. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBBR8k-GFPuv
Plasmid#106385PurposeL-arabinose-inducible araBAD promoter expressing gfpuv on BBR1 originDepositorInsertGFPuv
ExpressionBacterialPromoteraraBADAvailable SinceMarch 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pVSV eGFP RABV-G
Plasmid#31833DepositorInsertVSV genome
ExpressionBacterialAvailable SinceJuly 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
HA-mDyn3 pcDNA3
Plasmid#36265DepositorAvailable SinceApril 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
pSL1119 (pDonor)
Plasmid#160732PurposeMini-transposon donor plasmid for V. cholerae CAST system. pUC19 vector backbone.DepositorInsertVchCAST donor DNA (Mini-Tn)
ExpressionBacterialPromoterN/AAvailable SinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEcH6IleRS
Plasmid#177590PurposeExpresses His tagged IleRS in Escherichia coli cellsDepositorAvailable SinceFeb. 3, 2026AvailabilityAcademic Institutions and Nonprofits only -
pEcValRSH6
Plasmid#177600PurposeExpresses His tagged ValRS in Escherichia coli cellsDepositorAvailable SinceFeb. 3, 2026AvailabilityAcademic Institutions and Nonprofits only -
pET28b_RanWT
Plasmid#186270PurposeExpression vector of His-RanQ69L in E.coliDepositorInsertGTP-binding nuclear protein Ran (Ran Mouse)
TagsHis taggedExpressionBacterialPromoterT7 promoterAvailable SinceJuly 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJMP1356
Plasmid#119276Purposefor cloning new sgRNAsDepositorInsertdcas9
ExpressionBacterialMutationD10A and H840AAvailable SinceMarch 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTRC-LIC
Plasmid#62343PurposeUsed for tac promoter regulated high-level expression of cloned, untagged genes in E. Coli.DepositorTypeEmpty backboneExpressionBacterialPromotertac promoter from pKK233-2Available SinceMarch 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
LgBiT-ST
Plasmid#207633PurposeA plasmid encoding LgBiT fused to SpyTagDepositorInsertLgBiT-SpyTag
Tags6xHisExpressionBacterialPromoterT7Available SinceNov. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKL2310
Plasmid#187875PurposeEmpty pVS1-based INTEGRATE vector with loxP cargoDepositorTypeEmpty backboneUseCRISPRExpressionBacterialPromoterJ23119Available SinceAug. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pR0306 CcaCas13b His6-TwinStrep-SUMO
Plasmid#182687PurposeExpresses CcaCas13b with N terminal 6xHis-SUMO tag for recombinant bacterial expression and purificationDepositorInsertCcaCas13b
TagsSUMOExpressionBacterialPromoterT7Available SinceApril 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pZA31-sulA-GFP
Plasmid#78493PurposeExpression of GFP under the sulA promoter.DepositorInsertGFPmut2
ExpressionBacterialAvailable SinceApril 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDSG372
Plasmid#115602Purposesurface-displayed Nb1; medium copyDepositorInsertregulated adhesin construct
ExpressionBacterialAvailable SinceOct. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAmCyan1-C1-ADCY6
Plasmid#113905PurposeN-terminal Cyan Fluorescent tag in Adenylate Cyclase 6DepositorAvailable SinceOct. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC18T-mini-Tn7T-Gm-Pc-tdTomato
Plasmid#199250PurposeFluorescent labelling of bacterial cells with tdTomatoDepositorInsertCodon-optimized tdTomato gene
ExpressionBacterialMutationCodon optimization for XanthomonadaceaePromoterPc promoter from class III integron of Delftia a…Available SinceMay 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSUMO1
Plasmid#52258PurposeBacterial expression of human 6His-tagged SUMO1, SUMO-E1 and SUMO-E2DepositorTags6His-tagExpressionBacterialMutationC-terminal gly-glyPromoterT7 and rbsAvailable SinceJuly 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSH124-ssr
Plasmid#198025PurposeExpresses the gene codying for the Ssr recombinase from Pseudomonas putida DOT-T1EDepositorInsertT0 terminator - Ap/Cb resistance cassette
ExpressionBacterialPromoterApR promoterAvailable SinceOct. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pet-15b-microID
Plasmid#172881Purposeexpresses microID in E. coliDepositorInsertmicroID
TagsmycExpressionBacterialMutationfragment ([aa2-171]) of aquifex aeolicus BirA wit…PromoterT7Available SinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28b-zDjCas13d-His
Plasmid#234481PurposePlasmid for bacterial expression and purification of DjCas13d protein (zebrafish codon-optimized)DepositorInsertDjCas13d
UseCRISPRTags6xHisExpressionBacterialAvailable SinceApril 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pOSIP-KO (KanR, 186)
Plasmid#45985DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialAvailable SinceJuly 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET-45b-mCherry-NPM1
Plasmid#194546PurposeBacterial Expression of 6xHis mCherry-NPM1 Fusion ProteinDepositorAvailable SinceFeb. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRSET-GCaMP6f
Plasmid#67564Purposebacterial expression of a fluorescent calcium reporterDepositorInsertGCaMP6f
TagsHIS-tagExpressionBacterialAvailable SinceJuly 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
MAC3-tag-N
Plasmid#185482PurposeGateway compatible destination vectors containing StrepIII/HA/UltralDDepositorTypeEmpty backboneTagsStrepIII-HA-UltraIDExpressionBacterial and MammalianAvailable SinceJuly 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDae069 (eMutaT7[PmCDA1] with optimized RBS)
Plasmid#187621PurposeExpresses eMutaT7[PmCDA1] and UGI for mutagenizing genes downstream from a T7 promoter in e.coliDepositorInsertPmCDA1-T7RNAP
ExpressionBacterialAvailable SinceAug. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAJM.771
Plasmid#108534PurposeNahRAM +PSalTTC-YFP Reporter. Sal sensor for use in non-Marionette strain.DepositorInsertNahRAM + PSalTTC-YFP
ExpressionBacterialMutationNahR: I25V P72H V118A R248CAvailable SinceJuly 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSL1777 (pEffector)
Plasmid#160731PurposeEffector expression for V. cholerae CAST system, encodes all protein and RNA components. Entry vector encodes non-targeting crRNA, with BsaI sites for spacer cloning. pCDF backbone.DepositorInsertVchCAST proteins and crRNA
ExpressionBacterialPromoterJ23119Available SinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDuBir-Lbu-dCas13a-avitag
Plasmid#100817PurposeDual expression of Lbu-dCas13a with a C-terminal avi-tag and BirA to biotinylate Lbu-dCas13aDepositorInsertLbu_Cas13a_R472A_H477A_R1048A_H1053A
TagsAviTag and His6-MBPExpressionBacterialMutationR472A, H477A, R1048A, H1053APromoterT7Available SinceJuly 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMAL-c5x_UnaG
Plasmid#163125PurposeA plasmid with UnaG coding sequence cloned into it. UnaG gene codon optimized for E. coli and B. theta. Expresses UnaG with N-term maltose-binding protein (MBP)-tag and C-term His-tag.DepositorInsertUnaG
TagsHis and MBPExpressionBacterialAvailable SinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only