29,712 results
-
Plasmid#157790PurposeEcoRV digestion produces 12x601 chromatin assembly construct with 35 bp linker lengths in addition to carrier DNA that prevents overassembly of nucleosomesDepositorInsert12x601_35bp linker
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pOPARA1
Plasmid#200416PurposeGolden Gate assembly of expression constructs containing pBAD promoter (carbenicillin resistance)DepositorArticleTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterpBADAvailable sinceMay 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBEL2511
Plasmid#195625PurposeComplementation of MlaA(WT)DepositorInsertMlaA
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
A1-LCD+7R+12D
Plasmid#178678PurposeBacterial expression of N-terminally 6His tagged A1-LCD with seven Arg residues added, twelve Glu residues added (LCD: low-complexity domain, aa 186-320)DepositorInsertA1-LCD+7R+12D (HNRNPA1 Human)
UseTags6xHisExpressionBacterialMutationseven Arg residues added, twelve Glu residues add…PromoterAvailable sinceMarch 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRSET-iSeroSnFR
Plasmid#128483PurposeFluorescent reporter for serotonin (bacterial expression)DepositorInsertiSeroSnFR
UseTagsHis, T7, and cpsfGFPExpressionBacterialMutationPromoterT7Available sinceDec. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET23a_mod_HuD_FL
Plasmid#65751Purposeplasmid for bacterial overexpression of HuDDepositorInsertELAVL4 (ELAVL4 Human)
UseTags6X His and FLAG HAExpressionBacterialMutationPromoterT7Available sinceJune 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDL17
Plasmid#106483PurposepDL17 is based on conditionally replicating backbone derived from pQE-30 and expresses gam, bet and exo genes under control of PrhaB promoter for efficient dsDNA integration into E. coli chromosomeDepositorInsertsgam
bet
exo
lacI
rhaR
rhaS
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET His6 MBP N10 TEV LIC cloning vector (2C-T)
Plasmid#29706DepositorTypeEmpty backboneUseTagsHis6-MBP-N10-TEVExpressionBacterialMutationPromoterAvailable sinceAug. 17, 2011AvailabilityAcademic Institutions and Nonprofits only -
D. vulgaris sp2 CRISPR/pACYCDuet-1
Plasmid#81186PurposeExpresses CRISPR array containing 3 copies of Desulfovibrio vulgaris spacer 2 and flanking repeats in E. coliDepositorInsertSynthetic CRISPR array (3 copies of D. vulgaris spacer #2 flanked by repeats)
UseCRISPRTagsNoneExpressionBacterialMutationPromoterT7Available sinceSept. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAD43-daf-16 RNAi
Plasmid#34833DepositorInsertdaf-16 (daf-16 Nematode)
UseRNAiTagsExpressionBacterialMutationPromoterAvailable sinceJune 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
pLG008 (Ec78 retron)
Plasmid#157886PurposeExpresses the Ec78 retron defense systemDepositorInsertEc78 retron
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUS250
Plasmid#198322PurposeCumate-inducible gene expression in diverse gram negative bacteria. Features also include mobilisation via oriT, a gigantic multiple cloning site, and blue/white screening via amilCP chromoproteinDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromotercumate-inducibleAvailable sinceApril 12, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pUB-MSP1D1
Plasmid#161609PurposeBacterial expression of mutated membrane scaffold protein ("ultra-bright" MSP1D1) for Nanodisc formationDepositorInsertUD-MSP1D1
UseTags7-His tagExpressionBacterialMutationmutant of MSP1D1 ( Q138W, L181W, Y236W)PromoterT7Available sinceNov. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGRG36-Kn-PA1-GFP
Plasmid#79088PurposepGRG36-Kn-PA1-GFP is a Kanamycine resistance plasmid used to integrate a GFP expression cassette into the chromosome of E.coli, described in Yang et al. 2016 (Pending publication Pubmed ID)DepositorInsertGFP
UseBacterial chromosome integration and expression o…TagsExpressionBacterialMutationPromoterPA1Available sinceJuly 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUCP30T-eCFP
Plasmid#78476PurposeBroad range bacterial fluorescence labeling (ECFP)DepositorInserteCFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pet28a-His6-Halo-TEV-Nrf2
Plasmid#62455PurposeHalo-His6 tagged Nrf2 for E.coli expressionDepositorInsertNrf2 (NFE2L2 Human)
UseTagsHis6-Halo-TEVExpressionBacterialMutationPromoterAvailable sinceApril 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pNLS-2xBFP(4C)
Plasmid#176151PurposeEncodes NLS-2xBFP(4C), which is a tandem BFP with an N-terminal SV40 NLS. The protein has 4 solvent accessible cysteines for labeling with maleimide dyes.DepositorInsertNLS-2xBFP(4C)
UseTags6xHis tag and SV40 nuclear localization sequence …ExpressionBacterialMutationCompared with EGFP, the two BFP domains have the …PromoterPtrcAvailable sinceMarch 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPSU15
Plasmid#173828PurposeExpresses 15 kD Penn State ladder proteinDepositorInsertHSTPABPAC
UseTagsExpressionBacterialMutationPromoterT7Available sinceAug. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
MAC-tag-C
Plasmid#108077PurposeGateway compatible destination vectors containing StrepIII/HA/BioIDDepositorInsertCCDB (ccdB(letD) )
UseTagsStrepIII-HA-BirA*ExpressionBacterial and MammalianMutationPromoterAvailable sinceApril 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pETZZtevPP7CP(C68A,C73A)
Plasmid#27548DepositorInsertPseudomonas phage PP7 coat protein (cp Pseudomonas phage PP7)
UseTagsTEV protease cleavage site, six histidine tag, an…ExpressionBacterialMutationchanged two cysteine to alanine (C68A,C73A) to in…PromoterAvailable sinceMarch 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-v2 RsTAL At4CL
Plasmid#126524Purposeexpresses TAL (Rhodobacter sphaeroides) and 4CL (Arabidopsis thaliana paralog 1) for PYP chromophore biosynthesisDepositorInsertsUseTagsHisExpressionBacterialMutationPromoterT7Available sinceJune 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET51b(+)_MBP-nLucCP-67/68
Plasmid#113922PurposePlasmid encoding the bacterial expression of the MBP fused NanoLuc circular permuted between the position 67 and 68DepositorInsertMBP-NanoLucCP-67/68
UseLuciferaseTagsExpressionBacterialMutationPromoterT7Available sinceSept. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pL0-BvDODAα1
Plasmid#162528PurposeBeta vulgaris 4,5-DOPA dioxygenase alpha 1 coding sequence in pICH41308 MoClo Golden Gate level 0 acceptor for CDS1 modules.DepositorInsertDODAα1
UsePuc19-derivedTagsExpressionBacterialMutationPromoterAvailable sinceJuly 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28C-mCherry-HNRNPA1LCD
Plasmid#204405PurposeE. coli expression of HNRNPA1 low-complexity domain (186-320) with an N-terminal mCherry tagDepositorInsertHNRNPA1 (186-320) (HNRNPA1 Human)
UseTags6x-His; mCherryExpressionBacterialMutationPromoterT7Available sinceSept. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pP2-phiV10
Plasmid#196333PurposePlasmid expressing chimeric P2-phiV10 tail fiberDepositorInsertchimeric tail fiber P2-phiV10
UseTagsExpressionBacterialMutationPromoterP2 late promoterAvailable sinceJune 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDDGFP-A
Plasmid#40286DepositorInsertddGFP-A
UseTagsHis-tagExpressionBacterialMutationPromoterAvailable sinceSept. 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
pJS183
Plasmid#134142PurposeBacterial expression of GFP-Ska1 and Ska2DepositorInsertHis-GFP-human Ska1; human Ska2
UseTagsGFPExpressionBacterialMutationPromoterAvailable sinceFeb. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMA7-SacB
Plasmid#79968PurposeTM-MAGE strainDepositorInsertsLambda Red recombinase beta subunit
DNA adenine methylase
Levansucrase
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterpBADAvailable sinceSept. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRMCD28
Plasmid#167556PurposePlasmid pWSK29 encoding E. coli PhoA' for making reporter fusion proteins for topology mappingDepositorTypeEmpty backboneUseTagsPhoA'ExpressionBacterialMutationPromoterAvailable sinceMay 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28b(+)_rPD-L1nb
Plasmid#226715PurposeHeterologous expression and purification of recombinant PD-L1 nanobody (rPD-L1nb)DepositorInsertRecombinant PD-L1 nanobody
UseTagsHA tag and Histidine tagExpressionBacterialMutationPromoterT7Available sinceDec. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pETM33_Nsp4
Plasmid#156474PurposeBacterial expression of Sars-CoV2 Nsp4 protein with His-tag and GST-tagDepositorInsertNsp4 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)
UseTagsHis-GSTExpressionBacterialMutationGene insert is codon optimized for Ecoli.PromoterT7/LacOAvailable sinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAB151
Plasmid#101666PurposeOpto-T7RNAP*(563-F1)DepositorInsertOpto-T7RNAP*(563-F1)
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceSept. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCF525-EF1a-Hygro-P2A-mTagBFP2-lenti
Plasmid#115797PurposeExpresses mTagBFP in human cells, contained in a lentiviral vector.DepositorInsertmTagBFP
UseLentiviralTagsExpressionBacterial and MammalianMutationPromoterEF-1a promoterAvailable sinceOct. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
p324_DG
Plasmid#234690PurposeGolden gate domesticated pET28b with TEV-His6DepositorTypeEmpty backboneUseTagsTEV-His6ExpressionBacterialMutationNoPromoterAvailable sinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
p370_DG
Plasmid#234693PurposeGolden gate domesticated pET28b with 3C-mNeongreen-His10DepositorTypeEmpty backboneUseTags3C-mNeongreen-His10ExpressionBacterialMutationNoPromoterAvailable sinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
p277_DG
Plasmid#234695PurposeGolden gate domesticated pET28b for scarless cloning with His6-3CDepositorTypeEmpty backboneUseTagsHis6-3CExpressionBacterialMutationNoPromoterAvailable sinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
p357_DG
Plasmid#234700PurposeGolden gate domesticated pET28b for scarless cloning with His10-GST-3CDepositorTypeEmpty backboneUseTagsHis10-GST-3CExpressionBacterialMutationNoPromoterAvailable sinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
p368_DG
Plasmid#234691PurposeGolden gate domesticated pET28b with 3C-His6DepositorTypeEmpty backboneUseTags3C-His6ExpressionBacterialMutationNoPromoterAvailable sinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
p349_DG
Plasmid#234679PurposeGolden gate domesticated pET28b with His10-GST-3CDepositorTypeEmpty backboneUseTagsHis10-GST-3CExpressionBacterialMutationNoPromoterAvailable sinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
p351_DG
Plasmid#234681PurposeGolden gate domesticated pET28b with His10-mNeongreen-3CDepositorTypeEmpty backboneUseTagsHis10-mNeongreen-3CExpressionBacterialMutationNoPromoterAvailable sinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
p364_DG
Plasmid#234686PurposeGolden gate domesticated pET28b with His10-bdNEDD8 and OpsinDepositorTypeEmpty backboneUseTagsHis10-bdNEDD8 and OpsinExpressionBacterialMutationNoPromoterAvailable sinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
p369_DG
Plasmid#234692PurposeGolden gate domesticated pET28b with 3C-His10DepositorTypeEmpty backboneUseTags3C-His10ExpressionBacterialMutationNoPromoterAvailable sinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
p344_DG
Plasmid#234674PurposeGolden gate domesticated pET28b with His6-3CDepositorTypeEmpty backboneUseTagsHis6-3CExpressionBacterialMutationNoPromoterAvailable sinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
p367_DG
Plasmid#234689PurposeGolden gate domesticated pET28b with His10-bdNEDD8 and 2X Strep tagDepositorTypeEmpty backboneUseTags2X Strep tag and His10-bdNEDD8ExpressionBacterialMutationNoPromoterAvailable sinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
p354_DG
Plasmid#234697PurposeGolden gate domesticated pET28b for scarless cloning with His10-TEVDepositorTypeEmpty backboneUseTagsHis10-TEVExpressionBacterialMutationNoPromoterAvailable sinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
p358_DG
Plasmid#234701PurposeGolden gate domesticated pET28b for scarless cloning with His10-bdNEDD8DepositorTypeEmpty backboneUseTagsHis10-bdNEDD8ExpressionBacterialMutationNoPromoterAvailable sinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
p359_DG
Plasmid#234702PurposeGolden gate domesticated pET28b for scarless cloning with His10-mNeongreen-3CDepositorTypeEmpty backboneUseTagsHis10-mNeongreen-3CExpressionBacterialMutationNoPromoterAvailable sinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
p360_DG
Plasmid#234703PurposeGolden gate domesticated pET28b for scarless cloning with His10-mNeongreen-bdNEDD8DepositorTypeEmpty backboneUseTagsHis10-mNeongreen-bdNEDD8ExpressionBacterialMutationNoPromoterAvailable sinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
p346_DG
Plasmid#234676PurposeGolden gate domesticated pET28b with His10-TEVDepositorTypeEmpty backboneUseTagsHis10-TEVExpressionBacterialMutationNoPromoterAvailable sinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
p348_DG
Plasmid#234678PurposeGolden gate domesticated pET28b with His10-MBP-3CDepositorTypeEmpty backboneUseTagsHis10-MBP-3CExpressionBacterialMutationNoPromoterAvailable sinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only