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32,656 results

Showing: 3401 - 3450 of 32656 results

  1. hzHDAC10

    Plasmid
    #225542
    Purpose
    Expresses histone deacetylase 10 (HDAC10) humanized catalytic domain, also known as hzHDAC10, in Escherichia coli cells.
    Depositor
    David Christianson
    Article
    Herbst-Gervasoni et al ACS Chem Biol. 2020 Aug 21;15(8):2154-21
    Insert
    Histone Deacetylase 10 humanized catalytic domain (hdac10 Zebrafish)
    Tags
    His6-MBP tag with cleavable TEV site
    Expression
    Bacterial
    Mutation
    Mutated A24E and D94A
    Promoter
    T7
    Available Since
    Oct. 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    225542-plasmid-map-sequence-id-448447

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  4. EBOV 4cis

    Plasmid
    #249332
    Purpose
    Integration plasmid encoding EBOV essential replication factors (NP, VP35, VP30, and L protein)
    Depositor
    Amy Kistler
    Insert
    EBOV NP-P2A-VP35-P2A-VP30-P2A-L protein
    Use
    Piggybac donor vector
    Expression
    Bacterial and Mammalian
    Mutation
    the EBOV NP, VP35, and VP30 ORF sequences within …
    Promoter
    CAG
    Available Since
    Jan. 22, 2026
    Availability
    Academic Institutions and Nonprofits only
    249332-plasmid-map-sequence-id-497977

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  7. Kif1A-EGFP-SNAP

    Plasmid
    #229851
    Purpose
    Kif1A (aa1-351)-Kif1A neck linker(17aa) fused to Kin1 coiled coil (aa345-406)-EGFP-SNAP-his. This Kif1A was used to link an oligo to the motor using the SNAP tag for single molecule experiments
    Depositor
    William Hancock
    Insert
    Kif1A-EGFP-SNAP (Kif1a Rat)
    Tags
    6xHis, EGFP, and SNAP-tag
    Expression
    Bacterial
    Available Since
    Feb. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    229851-plasmid-map-sequence-id-460711

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  11. Pv-RNR-R2

    Plasmid
    #25589
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    Pv-RNR-R2
    Tags
    His
    Expression
    Bacterial
    Available Since
    Aug. 25, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  12. AzoRS-4

    Plasmid
    #182884
    Purpose
    A pEvol-based plasmid containing one inducible (by arabinose) copy of the AzoRS-4 aaRS. The 2nd constitutive aaRS copy typically found in pEvol plasmids was deleted.
    Depositor
    Miriam Amiram
    Article
    Israeli et al Adv. Funct. Mater. 2021, 31, 2011276
    Insert
    AzoRS-4
    Expression
    Bacterial
    Available Since
    July 5, 2022
    Availability
    Academic Institutions and Nonprofits only
    182884-plasmid-map-sequence-id-366058

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  15. pJA54 HLA-A*03:01

    Plasmid
    #180451
    Purpose
    E. coli expression of soluble MHC protein
    Depositor
    John Altman
    Insert
    HLA-A*03:01
    Tags
    BSP41
    Expression
    Bacterial
    Mutation
    Replacement of transmembrane domain with enzymati…
    Available Since
    March 2, 2022
    Availability
    Academic Institutions and Nonprofits only
    180451-plasmid-map-sequence-id-355758

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  17. pTF-ahpC-rfp

    Plasmid
    #153035
    Purpose
    For CRISPR-Cas12a genome editing in E. coli. Contains a constituitively expressed CRISPR array targeting the ahpC gene in E. coli and donor DNA to insert an rfp translational fusion in the ahpC gene.
    Depositor
    Nigel Scrutton
    Article
    Jervis et al Microb Biotechnol. 2021 Mar 12. doi: 10.
    Insert
    CRISPR array
    Expression
    Bacterial
    Available Since
    May 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    153035-plasmid-map-sequence-id-318838

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  18. pHN922

    Plasmid
    #178975
    Purpose
    Expression of GST-His8-GFP-nanobody
    Depositor
    Kazuhiro Abe
    Insert
    GST-His8-GFP-nanobody
    Use
    Affinity Reagent/ Antibody
    Tags
    GST, His8
    Expression
    Bacterial
    Promoter
    tac
    Available Since
    Feb. 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    178975-plasmid-map-sequence-id-354968

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  20. p405ADH1

    Plasmid
    #15966
    Depositor
    Nicolas Buchler, Fred Cross
    Insert
    p405ADH1 (ADH1 Budding Yeast)
    Expression
    Bacterial and Yeast
    Mutation
    ADH1 promoter is between SacI and XbaI restrictio…
    Available Since
    Nov. 2, 2007
    Availability
    Academic Institutions and Nonprofits only
    15966-plasmid-map-sequence-id-8451

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  22. pGLO-Magenta-AZA

    Plasmid
    #220784
    Purpose
    Expresses a magenta chromoprotein (visible light and fluorescence) with a his-tag and an inducible GFP (araBAD-promoter)
    Depositor
    Angela Leu
    Insert
    magenta chromoprotein
    Tags
    6xHis
    Expression
    Bacterial
    Available Since
    July 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    220784-plasmid-map-sequence-id-437914

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  23. pMSP3535VA

    Plasmid
    #46887
    Purpose
    Gram-Positive Bacterial Shuttle Vector for Nisin-Controlled Inducible Expression
    Depositor
    Gary Dunny
    Article
    Bryan et al Plasmid. 2000 Sep;44(2):183-90.
    Type
    Empty backbone
    Use
    Nisin-inducible expression; gram-positive bacteri…
    Expression
    Bacterial
    Promoter
    PnisA
    Available Since
    Sept. 18, 2013
    Availability
    Academic Institutions and Nonprofits only
    46887-plasmid-map-sequence-id-66434

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  25. pACYC-T7-SpCas9-T7-EGFP_gRNA1-T7term (BPK848)

    Plasmid
    #181745
    Purpose
    dual T7 promoter vector for bacterial expression of SpCas9 with a c-terminal NLS and 3xFLAG tag, along with an SpCas9 sgRNA targeting EGFP site 1
    Depositor
    Benjamin Kleinstiver
    Article
    Christie et al Nat Biotechnol. 2022 Oct 6. pii: 10.1038
    Insert
    human/zebrafish codon optimized SpCas9
    Use
    In vitro transcription; t7 promoter
    Tags
    NLS(SV40)-3xFLAG
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Feb. 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    181745-plasmid-map-sequence-id-354676

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  29. pMG024 MBP-GSK3β-HA-His, GST_λPPase

    Plasmid
    #196182
    Purpose
    Co-expresses MBP-GSK3β-HA-His (human GSK3β as a fusion protein with MBP, HA, and His-tags) and GST_λPPase in E.coli to produce unphosphorylated MBP-GSK3β-HA-His
    Depositor
    Jesse Zalatan
    Article
    Gavagan et al Elife. 2023 Aug 7;12:e85444. doi: 10.755
    Inserts
    GSK3B (GSK3B Human)
    λPPase
    Tags
    GST, HA, His6, and MBP
    Expression
    Bacterial
    Promoter
    Tac
    Available Since
    Jan. 31, 2023
    Availability
    Academic Institutions and Nonprofits only
    196182-plasmid-map-sequence-id-383259

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  32. pEXP5-NT/pT7 LuxR

    Plasmid
    #193625
    Purpose
    Constitutive T7 RNAP-mediated expression of LuxR transcription factor for Lux quorum sensing system. LuxR sequence is codon optimized for E. coli.
    Depositor
    Dora Tang
    Article
    Gonzales et al ChemSystemsChem 2023
    Insert
    LuxR
    Tags
    6xHis
    Expression
    Bacterial
    Promoter
    T7 RNAP promoter
    Available Since
    July 18, 2023
    Availability
    Academic Institutions and Nonprofits only
    193625-plasmid-map-sequence-id-388599

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  47. pTCF37 HLA-B*35:01

    Plasmid
    #180450
    Purpose
    E. coli expression of soluble MHC protein
    Depositor
    John Altman
    Insert
    HLA-B*35:01
    Tags
    BSP41
    Expression
    Bacterial
    Mutation
    Replacement of transmembrane domain with enzymati…
    Available Since
    March 29, 2022
    Availability
    Academic Institutions and Nonprofits only
    180450-plasmid-map-sequence-id-358091

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Showing: 3401 - 3450 of 32656 results