29,729 results

1. p356_DG

   Plasmid

   #234699

   Purpose

   Golden gate domesticated pET28b for scarless cloning with His10-MBP-3C

   Depositor

   Matthew Wohlever

   Article

   Gaur et al Protein Sci. 2025 Apr;34(4):e70106. doi:

   Type

   Empty backbone

   Use

   Tags

   His10-MBP-3C

   Expression

   Bacterial

   Mutation

   No

   Promoter

   Available since

   April 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. p345_DG

   Plasmid

   #234675

   Purpose

   Golden gate domesticated pET28b with His10-3C

   Depositor

   Matthew Wohlever

   Article

   Gaur et al Protein Sci. 2025 Apr;34(4):e70106. doi:

   Type

   Empty backbone

   Use

   Tags

   His10-3C

   Expression

   Bacterial

   Mutation

   No

   Promoter

   Available since

   April 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. p347_DG

   Plasmid

   #234677

   Purpose

   Golden gate domesticated pET28b with His10-Thrombin

   Depositor

   Matthew Wohlever

   Article

   Gaur et al Protein Sci. 2025 Apr;34(4):e70106. doi:

   Type

   Empty backbone

   Use

   Tags

   His10-Thrombin

   Expression

   Bacterial

   Mutation

   No

   Promoter

   Available since

   April 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. p355_DG

   Plasmid

   #234698

   Purpose

   Golden gate domesticated pET28b for scarless cloning with His10-Thrombin

   Depositor

   Matthew Wohlever

   Article

   Gaur et al Protein Sci. 2025 Apr;34(4):e70106. doi:

   Type

   Empty backbone

   Use

   Tags

   His10-Thrombin

   Expression

   Bacterial

   Mutation

   No

   Promoter

   Available since

   April 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. p352_DG

   Plasmid

   #234682

   Purpose

   Golden gate domesticated pET28b with His10-mNeongreen-bdNEDD8

   Depositor

   Matthew Wohlever

   Article

   Gaur et al Protein Sci. 2025 Apr;34(4):e70106. doi:

   Type

   Empty backbone

   Use

   Tags

   His10-mNeongreen-bdNEDD8

   Expression

   Bacterial

   Mutation

   No

   Promoter

   Available since

   April 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. p363_DG

   Plasmid

   #234685

   Purpose

   Golden gate domesticated pET28b with His10-bdNEDD8 and Sortase

   Depositor

   Matthew Wohlever

   Article

   Gaur et al Protein Sci. 2025 Apr;34(4):e70106. doi:

   Type

   Empty backbone

   Use

   Tags

   His10-bdNEDD8 and Sortase

   Expression

   Bacterial

   Mutation

   No

   Promoter

   Available since

   April 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. p353_DG

   Plasmid

   #234696

   Purpose

   Golden gate domesticated pET28b for scarless cloning with His10-3C

   Depositor

   Matthew Wohlever

   Article

   Gaur et al Protein Sci. 2025 Apr;34(4):e70106. doi:

   Type

   Empty backbone

   Use

   Tags

   His10-3C

   Expression

   Bacterial

   Mutation

   No

   Promoter

   Available since

   April 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pRecE564T

   Plasmid

   #226825

   Purpose

   Expressing RecE*T controled by Ptrc promoter

   Depositor

   Sheng Yang

   Article

   Wang et al ACS Synth Biol. 2025 May 8. doi: 10.1021

   Insert

   recE*T
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pISFba1

   Plasmid

   #226820

   Purpose

   Expressing ISEre1 controled by pCas promoter, λ-red recombinase, SacB and ISFba1 guide RNA targeting pMB1 ori

   Depositor

   Sheng Yang

   Article

   Zhou et al Biotechnol J. 2025 Mar;20(3):e70005. doi

   Insert

   ISFba1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pISFba1reRNA

    Plasmid

    #226822

    Purpose

    Expressing ISFba1 guide RNA targeting maeB site controled by PJ23119

    Depositor

    Sheng Yang

    Article

    Zhou et al Biotechnol J. 2025 Mar;20(3):e70005. doi

    Insert

    ISFba1 none target guide RNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pColdI-SBP-eIF4A1

    Plasmid

    #233621

    Purpose

    Expression of SBP-tagged eIF4A1 in E.coli

    Depositor

    Shintaro Iwasaki

    Article

    Chen et al Cell Chem Biol. 2021 Apr 15;28(4):475-48

    Insert

    SBP-eIF4A1 (EIF4A1 Human)
    

    Use

    Tags

    His6-SBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET-51b(+)-cpHaloTag141/145

    Plasmid

    #194660

    Purpose

    Protein production of circular permuted HaloTag (cpHaloTag141/145) in bacteria

    Depositor

    Kai Johnsson

    Article

    Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

    Insert

    cpHaloTag141/145
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET-51b(+)-cpHaloTag154/156

    Plasmid

    #194661

    Purpose

    Protein production of circular permuted HaloTag (cpHaloTag154/156) in bacteria

    Depositor

    Kai Johnsson

    Article

    Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

    Insert

    cpHaloTag154/156
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pELxG4

    Plasmid

    #172570

    Purpose

    Inducible gene expression, pET origin, LuxR activator, PLuxB promoter, GFPmut3, Carbenicillin Resistance Marker.

    Depositor

    Christopher Reisch

    Insert

    LuxR-GFPmut3
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PLuxB

    Available since

    Dec. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET28a-Hook3 aa 1-552

    Plasmid

    #74614

    Purpose

    Expresses a truncation of Hook3 in bacteria for purification

    Depositor

    Ron Vale

    Article

    Schroeder et al J Cell Biol. 2016 Aug 1;214(3):309-18. d

    Insert

    human Hook3 amino acids 1-552 (HOOK3 Human)
    

    Use

    Tags

    6x His, Strep II, and superfolder GFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET21-pelB-LaG-16

    Plasmid

    #172746

    Purpose

    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-16; HIS tag preceded by HRV 3C/PreScission cleavage site.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-16
    

    Use

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET28C-mCherry-DDX4LCD

    Plasmid

    #204408

    Purpose

    E. coli expression of DDX4 low-complexity domain (1-236) with an N-terminal mCherry tag

    Depositor

    Samie Jaffrey

    Article

    Dumelie et al Nat Chem Biol. 2023 Nov 16. doi: 10.1038

    Insert

    DDX4(1-236) (DDX4 Human)
    

    Use

    Tags

    6x-His; mCherry

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. T7-LacO-GFP_ColE1

    Plasmid

    #171648

    Purpose

    Expresses GFP in BL21(DE3) E. coli. Positive control for expression experiments. Contains ColE1 origin of replication.

    Depositor

    Tara Deans

    Article

    MacDonald et al Nat Commun. 2021 Jul 5;12(1):4109. doi:

    Insert

    T7-LacO-GFPssrA-T7 Stop
    

    Use

    Tags

    ssrA degradation tag (DAS+4)

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. R701-X54-566: His6-MBP-tev-Avi-Hs.KRAS4b(2-169) G12D

    Plasmid

    #159562

    Purpose

    E. coli protein expression of His6-MBP-tev-Avi-Hs.KRAS4b(2-169) G12D

    Depositor

    Dominic Esposito

    Article

    Gillette et al Sci Rep. 2015 Nov 2;5:15916. doi: 10.103

    Insert

    His6-MBP-tev-Avi-Hs.KRAS4b(2-169) G12D (KRAS Human)
    

    Use

    Tags

    His6-MBP-tev-Avi

    Expression

    Bacterial

    Mutation

    G12D

    Promoter

    Available since

    Sept. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Skp

    Plasmid

    #213139

    Purpose

    Expresses chaperone protein SKP

    Depositor

    Sebastian Hiller

    Article

    Rath et al Nat Commun. 2023 Sep 13;14(1):5648. doi:

    Insert

    SKP (21-161) (skp E.coli)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pLG004 (RT-Abi-P2)

    Plasmid

    #157882

    Purpose

    Expresses the RT-Abi-P2 defense system

    Depositor

    Feng Zhang

    Article

    Gao et al Science. 2020 Aug 28;369(6507):1077-1084

    Insert

    RT-Abi-P2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pGCP123-GFP_g1

    Plasmid

    #153518

    Purpose

    sgRNA to GFP gene (NT) under nisin-inducible nisA promoter, barcoded

    Depositor

    Kimberly Kline

    Article

    Afonina et al mBio. 2020 Oct 20;11(5):e01101-20. doi:

    Insert

    PnisA-sgRNA(GFP_g1)_dCas9 scaffold (barcoded)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pCVD364

    Plasmid

    #63739

    Purpose

    V cholerae 395 nanH gene cloned into the BamHI site of pCVD315

    Depositor

    James Kaper

    Article

    Vimr et al J Bacteriol. 1988 Apr;170(4):1495-504.

    Insert

    nanH
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 6, 2015

    Availability

    Academic Institutions and Nonprofits only

24. Sirius/pRSETB

    Plasmid

    #51956

    Purpose

    bacterial expression of Sirius, a pH-insensitive and photostable ultramarine fluorescent protein

    Depositor

    Takeharu Nagai

    Article

    Tomosugi et al Nat Methods. 2009 May;6(5):351-3. doi: 1

    Insert

    Sirius
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pScI_dCas9-CDA-UL

    Plasmid

    #108551

    Purpose

    Bacterial Target-AID UGI-LVA vector

    Depositor

    Akihiko Kondo

    Article

    Banno et al Nat Microbiol. 2018 Feb 5. pii: 10.1038/

    Insert

    dCas9-PmCDA1-UGI-LVA
    

    Use

    Tags

    Flag

    Expression

    Bacterial

    Mutation

    D10A and H840A for SpCas9

    Promoter

    lambda OR

    Available since

    Oct. 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pNAS1B

    Plasmid

    #61968

    Purpose

    Duet vector for co-expression of GFP and mCherry from PBAD and PLtetO promoters, respectively; parent vector for split mCherry assembly vectors

    Depositor

    Lynne Regan

    Article

    Sawyer et al ACS Chem Biol. 2014 Nov 21;9(11):2502-7.

    Inserts

    mCherry
    GFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Serine 65 to Glycine (GGT), Serine 72 to Alanine …

    Promoter

    PBAD and PLtetO

    Available since

    Jan. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pET23b-2E2-HA-mEGFP-6xHis

    Plasmid

    #129594

    Purpose

    The encoded protein is the anti-HA frankenbody variant fused with the mEGFP and His-tag. This plasmid is for bacteria protein expression under T7 promoter and protein purification with HisTrap.

    Depositor

    Tim Stasevich

    Article

    Zhao et al Nat Commun. 2019 Jul 3;10(1):2947. doi:

    Insert

    Anti-HA frankenbody variant-mEGFP (2E2-HA scFv-mEGFP)
    

    Use

    Tags

    6xHis-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pSumo-His6-SUMO-AtTPR1(1-209)

    Plasmid

    #177858

    Purpose

    Bacterial Expression of TPR1

    Depositor

    Karsten Melcher

    Article

    Ke et al Sci Adv. 2015 Jul 24;1(6):e1500107. doi:

    Insert

    TPR1 (TPR1 Mustard Weed)
    

    Use

    Tags

    6xHis-SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. ACACA

    Plasmid

    #39083

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    ACACA (ACACA Human)
    

    Use

    Tags

    His Tev

    Expression

    Bacterial

    Mutation

    codon-optimized

    Promoter

    Available since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

30. pUCP20 PilB

    Plasmid

    #162845

    Purpose

    Expression of PilB with a C-terminal histag

    Depositor

    Katrina Forest

    Insert

    PilB
    

    Use

    Tags

    Histidine tag (H6x)

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pTHSSe_43

    Plasmid

    #109241

    Purpose

    pSC101 var1 BbsI cloning vector

    Depositor

    Christopher Voigt

    Article

    Segall-Shapiro et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

    Insert

    sfGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PJ23105

    Available since

    Jan. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pMyBADNT-kan

    Plasmid

    #84691

    Purpose

    Protein expression in mycobacteria

    Depositor

    Matthias Wilmanns

    Article

    Beckham et al Protein Sci. 2020 Oct 2. doi: 10.1002/pr

    Type

    Empty backbone

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    AraC

    Available since

    Jan. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pUC18/promSau/Lysostaphin

    Plasmid

    #125766

    Purpose

    Production of staphylolytic protein lysostaphin from a constitutive promoter

    Depositor

    Benedykt Wladyka

    Article

    Madry et al Mol Biotechnol. 2019 Oct;61(10):774-782.

    Insert

    lysostaphin
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    sequence corresponding to the mature form protein

    Promoter

    pemIK constitutive promoter

    Available since

    Oct. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pFTK094-LM-AMA002.0

    Plasmid

    #171366

    Purpose

    Entry Vector compatible, fungal replicating, shuttle backbone vector from the Fungal Modular Cloning ToolKit.

    Depositor

    Arnold Driessen

    Article

    Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    n/a

    Promoter

    Available since

    April 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pTP261

    Plasmid

    #104166

    Purpose

    Bacterial expression plasmid of anti-GFP nanobody with 1x Cysteine for maleimide labeling

    Depositor

    Dirk Görlich

    Article

    Pleiner et al Elife. 2015 Dec 3;4:e11349. doi: 10.7554

    Insert

    Anti-GFP nanobody Enhancer PDB 3K1K (1x Cysteine)
    

    Use

    Tags

    14x Histidine tag and NEDD8 from Brachypodium dis…

    Expression

    Bacterial

    Mutation

    1x Cysteine located at bps 493-495 in full sequen…

    Promoter

    Available since

    Oct. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. RBD#4L

    Plasmid

    #193021

    Purpose

    hnRNP C RRM RNA binding domain inserted in between N415 and N416 of Loop 1 of LwaCas13a

    Depositor

    Xue Gao

    Article

    Yang et al Nat Chem Biol. 2022 Sep 22. pii: 10.1038

    Insert

    Cas13a
    

    Use

    Tags

    hnRNP C RRM

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pMSP1E2

    Plasmid

    #20063

    Depositor

    Stephen Sligar

    Article

    Denisov et al J Am Chem Soc. 2004 Mar 24. 126(11):3477

    Insert

    MSP1E2 (APOA1 synthetic gene)
    

    Use

    Tags

    6-His

    Expression

    Bacterial

    Mutation

    "extended" MSP1; contains repeats of he…

    Promoter

    Available since

    March 16, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pET21b-RBD-JA1

    Plasmid

    #167126

    Purpose

    Expresses SARS-CoV-2 RBD in bacterial system

    Depositor

    David Margulies

    Article

    Ahmad et al bioRxiv. 2021 Jan 27. doi: 10.1101/2021.

    Insert

    SARS-CoV-2 Receptor Binding domain (S SARS-CoV-2)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    residues (333 to 529 aa)

    Promoter

    T7

    Available since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. dCas9-CRY2Clust-BRD4ΔN

    Plasmid

    #200968

    Purpose

    induction of phase-separated condensate mediated by BRD4dN-IDR

    Depositor

    Jian Xu

    Article

    Kim et al Sci Adv. 2023 Mar 31;9(13):eadg1123. doi

    Insert

    BRD4dN
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Aug. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. SB_loop

    Plasmid

    #110103

    Purpose

    pBXPHM3 with scaffold Sybody of the loop library

    Depositor

    Markus Seeger

    Article

    Zimmermann et al Elife. 2018 May 24;7. pii: 34317. doi: 1

    Insert

    scaffold Sybody of the loop library
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    PBAD

    Available since

    May 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pET28 MBP NAAEF-AMPKa1 (13-476,529-550)-b2(76-272)-g1(24-327)

    Plasmid

    #177850

    Purpose

    Bacterial Expression of AMPK

    Depositor

    Karsten Melcher

    Article

    Yan et al Science. 2021 Jul 23;373(6553):413-419.

    Insert

    AMPK (a1b2g1)
    

    Use

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pYES1L-URA

    Plasmid

    #84301

    Purpose

    E.coli shuttle vector for large-scale DNA assembly in S. cerevisiae.

    Depositor

    George Church

    Article

    Ostrov et al Science. 2016 Aug 19;353(6301):819-22. d

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    May 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. CAMK2A

    Plasmid

    #38971

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    CAMK2A (CAMK2A Human)
    

    Use

    Tags

    His Tev

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

44. pBAD/HisB (TIREVOL)-sfGFP

    Plasmid

    #189675

    Purpose

    titratable expression of sfGFP in bacteria

    Depositor

    Daniel Daley

    Article

    Shilling et al Synth Biol (Oxf). 2022 Jul 5;7(1):ysac00

    Insert

    sfGFP
    

    Use

    Tags

    His-T7-EK

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available since

    Oct. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pET28a-mCherryGGBsMTS

    Plasmid

    #133625

    Purpose

    Expression vector for mCherry fused to a membrane targeting sequence (MTS) of the B.subtilis MinD (C-terminal fusion) and an N-terminal His-tag for purification.

    Depositor

    Petra Schwille

    Article

    Ramm et al Nat Commun. 2018 Sep 26;9(1):3942. doi:

    Insert

    mCherryGGBsMTS
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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46. pCRISPR-RFP entry

    Plasmid

    #192278

    Purpose

    Entry vector for cloning MmCas12m spacers. RFP flanked by MmCas12m CRISPR repeats can be digested out with BbsI.

    Depositor

    John van der Oost

    Article

    Wu et al Mol Cell. 2022 Dec 1;82(23):4487-4502.e7

    Insert

    RFP flanked by type V-M CRISPR repeat sequences
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PJ23119

    Available since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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47. pFNF

    Plasmid

    #22687

    Depositor

    Robert Benezra

    Article

    Nam et al Cell Stem Cell. 2009 Nov 6. 5(5):515-26.

    Insert

    Neo*
    

    Use

    Mouse Targeting

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    FRT-pPGK-pEM7-Neo*-bpA-FRT

    Promoter

    Available since

    Dec. 17, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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48. pOPTG-Tau-RING

    Plasmid

    #233691

    Purpose

    Expression of Tau-RING protein in bacteria

    Depositor

    Leo James

    Article

    Miller et al Cell. 2024 Oct 17;187(21):5967-5980.e17.

    Insert

    pOPTG
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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49. pAcrVIB1

    Plasmid

    #184566

    Purpose

    Inducible expression of anti-CRISPR protein AcrVIB1 in bacteria.

    Depositor

    Chase Beisel

    Article

    Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2

    Insert

    anti-CRISPR protein inhibiting Cas13b nucleases
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    Oct. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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50. pH6-TEV-spTorA-GFP(C)

    Plasmid

    #169041

    Purpose

    GFP preceeded by the signal peptide of TorA (spTorA) with an N-terminal 6xHis tag and TEV cleavage site in pBAD24.

    Depositor

    Siegfried Musser

    Article

    Bageshwar et al PLoS One. 2021 Sep 9;16(9):e0256715. doi

    Insert

    H6-spTorA-GFP
    

    Use

    Tags

    6xHis, TEV cleavage site, and spTorA (signal pept…

    Expression

    Bacterial

    Mutation

    The mut3 GFP variant with 5 additional mutations …

    Promoter

    araBAD

    Available since

    Oct. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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