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32,656 results

Showing: 3701 - 3750 of 32656 results

  9. pDule-pCNF

    Plasmid
    #85494
    Purpose
    Machinery plasmid expressing permissive pCNF synthetase and cognate amber suppressing tRNA. Incorporates azidoPhenylalanine.
    Depositor
    Ryan Mehl
    Article
    Miyake-Stoner et al Biochemistry. 2009 Jun 30;48(25):5953-62
    Insert
    para-cyanophenylalanine Mj synthetase
    Tags
    None
    Expression
    Bacterial
    Mutation
    Y32L L65V F108W Q109M D158G I159P
    Promoter
    lpp (constitutive)
    Available Since
    Jan. 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    85494-plasmid-map-sequence-id-169861

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  12. KEAP1 (KEAP1A-c029)

    Plasmid
    #110247
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    KEAP1 (KEAP1 Human)
    Tags
    His-TEV
    Expression
    Bacterial
    Promoter
    T7 - lacO
    Available Since
    June 7, 2018
    Availability
    Academic Institutions and Nonprofits only
    110247-plasmid-map-sequence-id-212283

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  16. pCasTet-λ

    Plasmid
    #128318
    Purpose
    Constitutive expression of cas9 and anhydrotetracycline/tetracycline inducible expression of lamda RED. Useful variant of Plasmid #62225 when arabinose induction is not possible.
    Depositor
    Christopher Johnston
    Article
    Johnston et al Proc Natl Acad Sci U S A. 2019 May 16. p
    Insert
    TetR and pTetR/TetO
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Mutation
    Constitutive expression of cas9 and anhydro-tetra…
    Promoter
    tet promoter
    Available Since
    Aug. 22, 2019
    Availability
    Academic Institutions and Nonprofits only
    128318-plasmid-map-sequence-id-254797

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  18. pBSV2G_P0826-BB0323-mCherryBb

    Plasmid
    #118244
    Purpose
    Kanamycin-resistant E.coli/B. burgdorferi shuttle vector; Encodes a BB0323-mCherry translational fusion under the control of the bb0826 promoter
    Depositor
    Christine Jacobs-Wagner
    Article
    Takacs et al Appl Environ Microbiol. 2018 Oct 12. pii
    Insert
    BB0323-mCherry translational fusion under the control of the bb0826 promoter
    Tags
    mCherry
    Expression
    Bacterial
    Available Since
    Dec. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    118244-plasmid-map-sequence-id-231804

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  20. His-FGE

    Plasmid
    #75147
    Purpose
    Expression vector for His(6) tagged Formylglycine Generating Enzyme (FGE) in bacterial host.
    Depositor
    Richard Fishel
    Article
    Liu et al Sci Rep. 2015 Nov 19;5:16883. doi: 10.10
    Insert
    FGE
    Tags
    Histidine tag and Thrombin
    Expression
    Bacterial
    Mutation
    N/A
    Promoter
    T7
    Available Since
    July 8, 2016
    Availability
    Academic Institutions and Nonprofits only

  31. pXW109Hg(RS-RinA-E11)2

    Plasmid
    #123151
    Purpose
    Mercury sensor with three-layered amplifier cascade. Need to work with plasmid pXWP11-gfp2. pSB4A3 carrying J109-32merR-t-PmerT-30hrpR-30hrpS-t-PhrpLE-30RinA(ASV)-t-PrinA-33ECF11-t
    Depositor
    Baojun Wang
    Article
    Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d
    Insert
    J109-32merR-t-PmerT-30hrpR-30hrpS-t-PhrpLE-30RinA(ASV)-t-PrinA-33ECF11-t
    Use
    Synthetic Biology
    Expression
    Bacterial
    Promoter
    J109, PmerT, PhrpLE, PrinA
    Available Since
    May 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    123151-plasmid-map-sequence-id-244015

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  33. pCN-mBFP

    Plasmid
    #63847
    Purpose
    Encodes a NADPH dependent blue fluorescent protein expressed under a strong promoter and strong RBS without His-tagged.
    Depositor
    Howard Salis
    Article
    Ng et al Metab Eng. 2015 May;29:86-96. doi: 10.10
    Insert
    mBFP
    Expression
    Bacterial
    Mutation
    Promoter and RBS was swapped. His-tag was removed…
    Available Since
    April 3, 2015
    Availability
    Academic Institutions and Nonprofits only
    63847-plasmid-map-sequence-id-106570

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  41. pYZ205

    Plasmid
    #104586
    Purpose
    Express SNAP-6His-CBD
    Depositor
    New England Biolabs, Yinhua Zhang
    Insert
    SNAP-6His-CBD
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Jan. 4, 2018
    Availability
    Industry, Academic Institutions, and Nonprofits
    104586-plasmid-map-sequence-id-266802

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  42. pHelper-CBE-sgRNA

    Plasmid
    #221137
    Purpose
    Helper plasmid for sgRNA cloning for CRISPR-Cas9 cytosine base editing. sgRNA-scaffold with Cas9 handle expressed from constitutive bacterial promoter J23119(SpeI). 2 BbsI sites for sgRNA cloning.
    Depositor
    Craig Roy
    Article
    Steiner et al mSphere. 2024 Nov 19:e0052324. doi: 10.1
    Insert
    sgRNA-scaffold with Cas9 handle
    Use
    CRISPR
    Expression
    Bacterial
    Promoter
    J23119(SpeI)
    Available Since
    Nov. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    221137-plasmid-map-sequence-id-436076

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Showing: 3701 - 3750 of 32656 results