32,069 results
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Plasmid#174724PurposeExpresses GFP-endoNA fusion protein for degradation of polysialic acid. The endoNA is a catalytically active endosialidase from bacteriophage PK1A.DepositorInsertGFP-endoNA fusion protein
TagsHistagExpressionBacterialPromoterT5Available SinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNR188
Plasmid#79543PurposeOutput plasmid: contains 16-state register made up of TP901, BxbI, and A118 recognition sites.DepositorTypeEmpty backboneExpressionBacterialAvailable SinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
gZiPro
Plasmid#198441PurposeActivity/inhibition assays, 19F-NMRDepositorInsertglycine linked Zika virus NS2B-NS3
TagsHis6-TEVExpressionBacterialMutationR95*A,PromoterT7 promoterAvailable SinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T-1-3xFLAG-HSP27
Plasmid#129229PurposeEncodes FLAG tagged human HSP27 for purification by GST affinityDepositorAvailable SinceJan. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLG012 (DRT type 4)
Plasmid#157890PurposeExpresses the DRT type 4 defense systemDepositorInsertDRT type 4
ExpressionBacterialAvailable SinceJan. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLG013 (DRT type 5)
Plasmid#157891PurposeExpresses the DRT type 5 defense systemDepositorInsertDRT type 5
ExpressionBacterialAvailable SinceNov. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pT7II-1N3Rtau
Plasmid#249552PurposeRecombinant protein synthesis of 1N3R isoform of human TauDepositorInsertTau (1N3R) (MAPT Human)
ExpressionBacterialAvailable SinceJan. 22, 2026AvailabilityAcademic Institutions and Nonprofits only -
pT7II-0N3Rtau
Plasmid#249553PurposeRecombinant protein synthesis of 0N3R isoform of human TauDepositorInsertTau (0N3R) (MAPT Human)
ExpressionBacterialAvailable SinceJan. 22, 2026AvailabilityAcademic Institutions and Nonprofits only -
pT7II-1N4Rtau
Plasmid#249554PurposeRecombinant protein synthesis of 1N4R isoform of human TauDepositorInsertTau (1N4R) (MAPT Human)
ExpressionBacterialAvailable SinceJan. 22, 2026AvailabilityAcademic Institutions and Nonprofits only -
pMAL-c2X_MBP-LcgcN(C24S)-H6
Plasmid#245527PurposeMaltose binding protein N-terminally fused to His-tagged N-terminal LCGC14 PolB2split intein fragmentDepositorInsertN-terminal Fragment of the LCGC14 Split Intein with C24S Mutation
TagsHexahistidine Tag and Maltose Binding Protein (MBā¦ExpressionBacterialMutationC24S within the N-terminal fragment of the LCGC14ā¦PromotertacAvailable SinceNov. 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
ZIKV isolate VE_Ganxian2016 NS2B-NS3 protease fusion with non cleavable linker (N-terminal Twin-Strep-tag)
Plasmid#228661PurposeBacterial expression of the fusion of the NS2B-NS3 protease from Zika virus for biochemical assayDepositorInsertZika virus portion of NS2B protease fused to NS3
TagsHis-Twin Strep-TevExpressionBacterialAvailable SinceFeb. 18, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
USUV strain Vienna 2001 NS2B-NS3 protease fusion with non cleavable linker
Plasmid#228658PurposeBacterial expression of the fusion of the NS2B-NS3 protease from Usutu virus for biochemical assayDepositorInsertUsutu virus portion of NS2B protease fused to NS3
TagsHis-Twin Strep-TevExpressionBacterialAvailable SinceFeb. 18, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
POWV strain LB NS2B-NS3 protease fusion with non cleavable linker
Plasmid#228654PurposeBacterial expression of the fusion of the NS2B-NS3 protease from Powassan virus for biochemical assayDepositorInsertPowassan virus portion of NS2B protease fused to NS3
TagsHis-Twin Strep-TevExpressionBacterialMutationAlanine mutant to stop self-cleaving ..VEREEA*AL.ā¦Available SinceFeb. 18, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
MVEV strain MVE-1-51 NS2B-NS3 protease fusion with non cleavable linker
Plasmid#228653PurposeBacterial expression of the fusion of the NS2B-NS3 protease from Murray Valley encephalitis virus for biochemical assayDepositorInsertMVEV portion of NS2B protease fused to NS3
TagsHis-Twin Strep-TevExpressionBacterialAvailable SinceFeb. 18, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
Fusion of West Nile NS2B-NS3 proteins
Plasmid#204796PurposeBacterial expression of a fusion of West Nile NS2B-NS3 proteinsDepositorInsertWest Nile NS2B-NS3 fusion
ExpressionBacterialAvailable SinceApril 25, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
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pCarCBADE
Plasmid#117757PurposeCodon optimized CarCBADE enzymes from Pectobacterium carotovorum in pBbaA5k backboneDepositorInsertCodon optimised CarCBA, Protein CarD, Ferredoxin CarE
ExpressionBacterialPromoterPLac(UV5)Available SinceNov. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
p5068 pGEX-6P-1 Brd4 full-length
Plasmid#14447DepositorAvailable SinceMarch 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SpyTag002-MBP
Plasmid#102831PurposeBacterial expression of SpyTag002 fused to maltose binding proteinDepositorInsertSpyTag002-MBP
TagsHis6 and Thrombin cleavage siteExpressionBacterialMutationPeptide tag added to the N-terminus of MBP via a ā¦PromoterT7Available SinceDec. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
GST-GRP78-Full Length (pGEX4T1)
Plasmid#238421PurposeExpresses GRP78 Full Length fused to the GST TagDepositorAvailable SinceAug. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRSF-G1TFAKRS
Plasmid#177311PurposetRNA synthetase/tRNA pair for the in vivo incorporation of N6-(trifluoroacetyl)-L-lysine (TFA-Lys) into proteins in E. coli in response to the amber (TAG) codonDepositorInserttRNA synthetase
ExpressionBacterialPromoterT7Available SinceNov. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJL NarL YdfI Flag
Plasmid#225650PurposeExpresses NarL with a mutated DNA binding domain (YdfI). For use with NarX chimeras and reporter plasmids from this study.DepositorInsertNarL-YdfI (narL Synthetic)
UseCell free expressionTagsFLAGExpressionBacterialMutationNarL with native DNA-binding domain replaced withā¦PromoterT7Available SinceDec. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTKEI-Tre-C04
Plasmid#79754PurposeSingle fluorescent protein biosensor for trehaloseDepositorInsertTre-C04
Tags6xHisExpressionBacterialPromoterpTrcAvailable SinceJuly 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTdTomato-L5
Plasmid#140994PurposetdTomato, StrepR, L5int, attP to create RFP mycobacteriaDepositorInserttdTomato
ExpressionBacterialPromoterhsp60Available SinceOct. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTlpA39-Wasabi
Plasmid#86116PurposemWasabi reporter gene gated by TlpA39DepositorInsertsTlpA39
mWasabi
Tags6xHisExpressionBacterialMutationD341A, D135V, A217V, L236FPromoterpTlpA and pTlpA, LacIAvailable SinceFeb. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMAL-his-LbCpf1-EC
Plasmid#79008PurposeBacterial expression - MBP-his tagged LbCpf1 (E.coli codon optimized)DepositorInsertLbCpf1
TagsMBP-6xHis-NLSExpressionBacterialPromotertac promoterAvailable SinceJuly 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
phage UbiC SNAP-DDX6
Plasmid#119948PurposeLentiviral vector expressing SNAP-DDX6 fusion proteinDepositorAvailable SinceMarch 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
iMbody_pSANG10-3F
Plasmid#184496PurposeExpresses iMbody-6xHis-3xFLAG in BL21(DE3) bacteriaDepositorInsertiMbody
Tags3xFLAG, 6xHis, and pelB leaderExpressionBacterialAvailable SinceNov. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJJH1295
Plasmid#36914DepositorInsert3x mCherry
ExpressionBacterial, Mammalian, and Yā¦Available SinceAug. 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
pUC18T-mini-Tn7T-Tp-ecfp
Plasmid#65034PurposeTpR mini-Tn7 vector, mobilizable, for CFP tagging bacteriaDepositorInsertecfp
ExpressionBacterialAvailable SinceNov. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGNW2
Plasmid#122086Purposesuicide vector for integration into Pseudomonas genome after insertion of homologous regions at the multiple cloning site; successful integration causes green fluorescence in cellsDepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialAvailable SinceApril 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
TXNRD1
Plasmid#38863PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-1_VNp-mNeongreen-StefinA
Plasmid#182419PurposeBacterial expression of Vesicle Nucleating peptide-mNeongreen-stefinA fusionDepositorInsertVNp-mNeongreen-stefin (CSTA Human)
TagsVesicle Nucleating peptide (VNp) & mNeongreenExpressionBacterialPromoterT7Available SinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSW002-Pc-DsRed-Express2
Plasmid#111251PurposeBroad host-range bacterial expression vector with constitutive Pc promoter; for tagging bacteria with DsRed-Express2DepositorInsertDsRed-Express2
ExpressionBacterialPromoterPcAvailable SinceAug. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
B-gTEMP/pRSET-b
Plasmid#188446PurposeB-gTEMP for bacterial expressionDepositorInsertB-gTEMP
Tags6xHis-tagExpressionBacterialAvailable SinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIA33
Plasmid#120803PurposeE. coli-C. difficile shuttle vector for CRISPR interference (CRISPRi) in C. difficile; sgRNA targets rfp; Pxyl::dCas9-opt Pgdh::sgRNA-rfpDepositorInsertsdeactivated nuclease dCas9, codon-optimized for C. difficile
single guide RNA targeting red fluorescent protein
UseCRISPRExpressionBacterialMutationBase-pairing region targets red fluorescent proteā¦PromoterPgdh and PxylAvailable SinceFeb. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKJ0463
Plasmid#228715PurposeBacterial expression of epT7 RNAP; for protein production and purificationDepositorArticleInsertSumo_epT7
ExpressionBacterialMutationT3M/G47A/E643GAvailable SinceNov. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET Flag TEV LIC cloning vector (2L-T)
Plasmid#29715DepositorTypeEmpty backboneTagsFLAG-TEVExpressionBacterialAvailable SinceJune 9, 2011AvailabilityAcademic Institutions and Nonprofits only -
CD11b promoter in pGEM3zf(-)
Plasmid#26168DepositorInsertCD11b promoter (ITGAM Human)
ExpressionBacterialAvailable SinceSept. 15, 2010AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6p-2-EWSR1
Plasmid#46384DepositorAvailable SinceJuly 29, 2013AvailabilityAcademic Institutions and Nonprofits only