29,729 results
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Plasmid#141140PurposeExpression of holo-[acyl-carrier-protein] synthaseDepositorInsertacpS
UseTagsMBPExpressionBacterialMutationPromoterAvailable sinceMay 28, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMBEC8
Plasmid#187599PurposeBase editing plasmid, constitutively expressing cytosine base editor and cas6. Contains GFP, flanked by BsaI restriction sites to introduce spacer and gRNAs. Apramycin resistanceDepositorInsertnCas9-BEC-UGI; cas6f, gfp
UseTagsExpressionBacterialMutationPromotertrcAvailable sinceJuly 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
His-CL7_MBP_1xNLS_iGeoCas9(C2)_2xNLS(star)
Plasmid#222991PurposeBacterial expression of iGeoCas9(C2) constructDepositorInsertHis-CL7_MBP_1xNLS_iGeoCas9(C2)_2xNLS(star)
UseCRISPRTagsCL7, His, and MBPExpressionBacterialMutationPromotertacAvailable sinceJuly 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
AdML
Plasmid#11242DepositorInsertAdML
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 14, 2006AvailabilityAcademic Institutions and Nonprofits only -
pDL52 (clone45)
Plasmid#188578PurposeHigh-yield production of coenzyme F420 in E. coliDepositorInsertsribA
ribD
yigB
fbiC
cofC
cofD
cofI
cofE
UseTagsExpressionBacterialMutationCodon optimization, synthetic ribosome binding si…PromoterT7 variantsAvailable sinceSept. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAT207
Plasmid#134884PurposeBacterial expressionDepositorInsertmRFP1
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJuly 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET-His-Dre
Plasmid#123130PurposeBacterial expression plasmid for wild-type Dre with an N-terminal His-tag.DepositorInsertDre recombinase
UseTags6x His tagExpressionBacterialMutationPromoterT7Available sinceApril 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBAD-pHuji
Plasmid#61555PurposeRed fluorescent protein based pH indicator in bacterial expression vectorDepositorInsertpHuji
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceDec. 19, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCRISPomyces-FnCpf1
Plasmid#129554PurposeContains codon-optimized FnCpf1 and gRNA for streptomycesDepositorInsertFnCpf1
UseTagsExpressionBacterialMutationPromoterrpsL(XC)-BbsIAvailable sinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEx-4T-1-YTHDF2
Plasmid#52301PurposeEncode YTHDF2 isoform 2 with N-terminal GST tag for E. coli expressionDepositorInsertYTHDF2 (YTHDF2 Human)
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceApril 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET28a-PHGDH
Plasmid#74113PurposeExpression of N-terminal His-taggedDepositorInsertPHGDH (PHGDH Human)
UseTagsHis-taggedExpressionBacterialMutationPromoterT7 promoterAvailable sinceJan. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pACYC-T7-Nla-repeat-BbsI
Plasmid#180215PurposeFor cloning Neisseria lactamica Type I-C crRNA for protein purification in E.ColiDepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterialMutationPromoterT7Available sinceMarch 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET29_LuxSit_i
Plasmid#194299PurposeBacterial expression of the de novo luciferase, LuxSit-iDepositorInsertLuxSit-i
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pASTA3
Plasmid#24657DepositorInserttdTomato
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 9, 2010AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T-1_WT_OCT4
Plasmid#40633DepositorInsertPOU5F1 (POU5F1 Human)
UseTagsGST, TEV cleavage, and Thrombin cleavageExpressionBacterialMutationPromotertacAvailable sinceJuly 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
pJBL7004
Plasmid#136943PurposeMalachite green aptamer, T7 promoterDepositorInsertmalachite green aptamer (MGA)
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterpT7Available sinceFeb. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pENO1-dTom-NATr
Plasmid#52208PurposeConstitutive expression of Candida codon-optimized dTomato with NAT selectable markerDepositorInsertdTomato
UseTagsExpressionBacterial and YeastMutationcodon optimized for Candida speciesPromoterENO1Available sinceMarch 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pUG-natNT2
Plasmid#110922Purposeexpression of nat gene conferring resistance to nourseothricin for gene deletion in yeastDepositorInsertnat
UseTagsExpressionBacterial and YeastMutationPromoterAgTEF1Available sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pST44-AP-1 core
Plasmid#184809PurposeBacterial expression of clathrin adaptor AP-1 core complexDepositorInsertHs AP1S3; Hs AP1B1 (1-584); Mm AP1G1 (1-595); Mm AP1M1
UseTagsGST tag and His6ExpressionBacterialMutationAP1S3: S148C; AP1B1: K359R, E476KPromoterT7Available sinceJune 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
BMP2K
Plasmid#73251PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertBMP2KA (BMP2K Human)
UseTagsHis6-Zb-TEVExpressionBacterialMutationpfam00069:Pkinase 52-311PromoterT7Available sinceApril 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET mOrange LIC cloning vector (u-mOrange)
Plasmid#29770DepositorTypeEmpty backboneUseTagsmOrangeExpressionBacterialMutationPromoterAvailable sinceJune 9, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCI-neo-sOVA
Plasmid#25098DepositorInsertsoluble ovalbumin
UseTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceJuly 1, 2010AvailabilityAcademic Institutions and Nonprofits only -
pDEST17-Cdc34a
Plasmid#18674DepositorInsertCdc34A (CDC34 Human)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceJune 11, 2008AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-puro-TagBFP-β-ACTIN-3′UTR-8xMS2
Plasmid#205160Purposeexpress β-ACTIN-3′UTR-8xMS2DepositorInsertβ-ACTIN-3′UTR-24xMS2
UseTagstagBFPExpressionBacterial and MammalianMutationPromoterCMVAvailable sinceOct. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX-Pak1 83-149
Plasmid#12216DepositorInsertPak1 (Pak1 Rat)
UseTagsGSTExpressionBacterialMutationDominant negative Pak1: autoinhibitory domain, aa…PromoterAvailable sinceAug. 11, 2006AvailabilityAcademic Institutions and Nonprofits only -
pTetO-ISce1-TetO
Plasmid#58506PurposeISce1 restriction site is flanked by Tet operator repeats, 96 on each sideDepositorInsertTet repeats flanking ISce1 restriction site
UseTagsExpressionBacterialMutationPromoterno promoterAvailable sinceJan. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
p406CYC1
Plasmid#15975DepositorInsertCYC1 promoter + polylinker + CYC1 terminator (CYC1 Budding Yeast)
UseTagsExpressionBacterial and YeastMutationCYC1 promoter is between SacI and XbaI restrictio…PromoterAvailable sinceNov. 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
pBAD/His-miRFP703
Plasmid#79985PurposeMonomeric near-infrared fluorescent protein miRFP703 in bacterial plasmidDepositorInsertmiRFP703
UseTagsExpressionBacterialMutationPromoteraraBAvailable sinceJuly 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET28a-AncMEcoGII250
Plasmid#205025PurposeExpressing engineered M.EcoGII with higher thermostabilityDepositorInsertAncestral M.EcoGII 250
UseTagsHis6x tagExpressionBacterialMutationPromoterT7Available sinceOct. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pbacPuroR
Plasmid#26348DepositorInsertBacterial promoter::PuroR::bacterial 3p flank
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 3, 2010AvailabilityAcademic Institutions and Nonprofits only -
6xHis(Thrombin)-GFP(TEV)-Drp1-StrepII
Plasmid#174424PurposeExpresses human Drp1 Isoform 3 with EGFP at N-terminus in bacteriaDepositorInsertDrp1
UseTags6xHis(Thrombin)EGFP(TEV) and StrepIIExpressionBacterialMutationNonePromoterT7Available sinceOct. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pScaf-3024.1
Plasmid#111404PurposepScaf phagemid with insert for generating DNA origami scaffold 3024 bases in lengthDepositorInsertpScaf-3024
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCON1-ProD.gfp
Plasmid#112518PurposeProD driven gfpmut3 expression with synTTDepositorInsertProD gfpmut3 TT
UseTagsExpressionBacterialMutationPromoterProDAvailable sinceJuly 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a_MBP_RBFOX2
Plasmid#224292PurposeMBP-RBFOX2 protein purification from bacteriaDepositorInsertRNA binding fox-1 homolog 2 (RBFOX2 Human)
UseTags6xHis and MBPExpressionBacterialMutationPromoterT7 promoterAvailable sinceAug. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-RhoA-wt_mScarlet-i_SGFP2
Plasmid#85071PurposeGreen/red FRET biosensor to monitor RhoA nucleotide loading state and activityDepositorInsertRhoA Biosensor wildtype with mScarlet-i and SGFP2 (RHOA Human)
UseTags6xHis and mScarlet-i and SGFP2ExpressionBacterial, Insect, and Mamm…MutationPromoterCMVAvailable sinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDMS197
Plasmid#43831DepositorTypeEmpty backboneUseBacterial allelic exchange vector with sacb1TagsExpressionBacterialMutationPromoterAvailable sinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGEX6p1-GST-Rnf31
Plasmid#63134PurposeExpression of human RNF31/HOIP codon optimized for E. coli and insect cellsDepositorInsertRnf31 (RNF31 Human, Synthetic)
UseTagsGSTExpressionBacterialMutationSynthetic based on human protein sequencePromotertacAvailable sinceMarch 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pPGC-K
Plasmid#174581PurposepOPIN-GG expression vector compatible with C-terminal tagging, Kanamycin resistantDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET30-T7-His-sfGFP
Plasmid#180755PurposepET30 vector missing lacO with sfGFP-S-tag inserted between the Bgl II and T7 terminatorDepositorInsertsuperfolder green fluorescent protein
UseTagsstrep tagExpressionBacterialMutationPromoterAvailable sinceJuly 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGNW2
Plasmid#122086Purposesuicide vector for integration into Pseudomonas genome after insertion of homologous regions at the multiple cloning site; successful integration causes green fluorescence in cellsDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceApril 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBHM2641
Plasmid#229525PurposeCarries OsTIR1-t2a-mNG-AID*, used to insert TIR1 expressing construct into the C. neoformans genome.DepositorInsertOsTIR1
UseCRISPRTagst2a-mNeonGreen-AID*ExpressionBacterial and YeastMutationF74GPromoterTEF1Available sinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBHM2642
Plasmid#229526PurposeCarries OsTIR1-t2a-mNG-mIAA7_opt, used to insert TIR1 expressing construct into the C. neoformans genome.DepositorInsertOsTIR1
UseTagst2a-mNeonGreen-mIAA7_optExpressionBacterial and YeastMutationF74GPromoterAvailable sinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBHM2644
Plasmid#229528PurposeCarries AID* tag, empty sgRNA, and NATR markerDepositorInsertAID* - empty sgRNA - NATR
UseTags2xFLAGExpressionBacterial and YeastMutationPromoterAvailable sinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBHM2645
Plasmid#229529PurposeCarries mIAA7_opt tag, empty sgRNA, and NATR markerDepositorInsertmIAA7_opt - empty sgRNA - NATR
UseCRISPRTags2xFLAGExpressionBacterial and YeastMutationPromoterAvailable sinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBHM2646
Plasmid#229530PurposeCarries mAID_opt tag, empty sgRNA, and NATR markerDepositorInsertmAID_opt - empty sgRNA - NATR
UseTags2xFLAGExpressionBacterial and YeastMutationPromoterAvailable sinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBHM2647
Plasmid#229989PurposeCarries OsTIR1-t2a-mNG-mIAA7, used to insert TIR1 expressing construct into the C. neoformans genome.DepositorInsertOsTIR1
UseTagst2a-mNeonGreen-mIAA7_optExpressionBacterial and YeastMutationF74GPromoterAvailable sinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBHM2648
Plasmid#229990PurposeCarries OsTIR1-t2a-mNG-mIAA7, used to insert TIR1 expressing construct into the C. neoformans genome.DepositorInsertOsTIR1
UseTagst2a-mNeonGreen-mIAA7_optExpressionBacterial and YeastMutationF74GPromoterAvailable sinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBHM2649
Plasmid#229991PurposeCarries OsTIR1-t2a-mNG-mIAA7, used to insert TIR1 expressing construct into the C. neoformans genome.DepositorInsertOsTIR1
UseTagst2a-mNeonGreen-mIAA7_optExpressionBacterial and YeastMutationF74GPromoterAvailable sinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBHM2650
Plasmid#229992PurposeCarries AID* tag, empty sgRNA, and HYGR markerDepositorInsertAID* - empty sgRNA - HYGR
UseTags2xFLAGExpressionBacterial and YeastMutationPromoterAvailable sinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits only