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32,656 results

Showing: 4201 - 4250 of 32656 results

  4. pBBR1-Rha-ETh_tji49-kan

    Plasmid
    #166669
    Purpose
    Genome engineering of Burkholderia
    Depositor
    Jun Fu
    Insert
    ETh_tji4
    Expression
    Bacterial
    Available Since
    April 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    166669-plasmid-map-sequence-id-329553

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  6. pR6K-GFP-RFP

    Plasmid
    #199652
    Purpose
    R6K backbone with Golden Gate (GG) cloning sites
    Depositor
    IIya Finkelstein
    Type
    Empty backbone
    Expression
    Bacterial
    Promoter
    J23119 promoter
    Available Since
    May 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    199652-plasmid-map-sequence-id-412250

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  11. pM2s2TsR

    Plasmid
    #137923
    Purpose
    E. coli Nissle pMUT2-derived plasmid with tetracycline resistance and constitutive RFP expression
    Depositor
    Neel Joshi
    Article
    Kan et al ACS Synth Biol. 2020 Dec 10. doi: 10.102
    Insert
    mRFP1
    Use
    Synthetic Biology; For use with e. coli nissle in…
    Expression
    Bacterial
    Promoter
    J23101
    Available Since
    Jan. 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    137923-plasmid-map-sequence-id-322897

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  22. pOPS0377

    Plasmid
    #115509
    Purpose
    Reporter plasmid suitable for experiments in environments where there is no antibiotic selection present. Constitutive promoter pNeo expressing sfGFP
    Depositor
    Philip Poole
    Article
    Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi
    Insert
    sfGFP (pOGG037) and T-pharma (pOGG003) assembled in pOGG026
    Expression
    Bacterial
    Mutation
    Domesticated for Golden Gate cloning
    Promoter
    Constitutive promoter pNeo pOGG001
    Available Since
    Oct. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    115509-plasmid-map-sequence-id-224290

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  24. pCCM’

    Plasmid
    #162709
    Purpose
    pCCM reconstructed after selection for CCMB1 growth on glycerol under ambient air
    Depositor
    David Savage
    Article
    Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1
    Insert
    Second CCM operon cloned from H. neapolitanus
    Expression
    Bacterial
    Mutation
    p15A origin replaced with high-copy colE1 origin
    Promoter
    PLtet0-1 promoter
    Available Since
    Jan. 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    162709-plasmid-map-sequence-id-341131

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  27. pDL17

    Plasmid
    #106483
    Purpose
    pDL17 is based on conditionally replicating backbone derived from pQE-30 and expresses gam, bet and exo genes under control of PrhaB promoter for efficient dsDNA integration into E. coli chromosome
    Depositor
    Sergey Sineoky
    Article
    Bubnov et al J Microbiol Methods. 2018 Jun 7. pii: S0
    Inserts
    gam
    bet
    exo
    lacI
    rhaR
    rhaS
    Expression
    Bacterial
    Available Since
    June 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    106483-plasmid-map-sequence-id-334156

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  30. pJMP3664

    Plasmid
    #222350
    Purpose
    Expression vector with PabstBR promoter, aprR marker, and replicates in A. baumannii and E. coli; empty vector plasmid with MCS for cloning - we recommend NcoI/BamHI.
    Depositor
    Jason Peters
    Article
    Bacon et al Microbiol Spectr. 2024 Nov 5;12(11):e013
    Type
    Empty backbone
    Expression
    Bacterial
    Available Since
    July 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    222350-plasmid-map-sequence-id-439070

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  33. pOP3MT

    Plasmid
    #112601
    Purpose
    Used for expression of protein in E. coli as TEV cleavable N-terminal Avi-His8-tag and MBP fusions
    Depositor
    Marko Hyvönen
    Type
    Empty backbone
    Tags
    Avi - 8His-MBP
    Expression
    Bacterial
    Available Since
    Aug. 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    112601-plasmid-map-sequence-id-220215

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  35. HA-HA-AR-DBD-LBD

    Plasmid
    #171237
    Purpose
    Mammalian expression of 2xHA-tagged human androgen receptor trancate: AR DNA-bindind domain and Ligand-binding domain (DBD-LBD)
    Depositor
    Steven Balk
    Article
    Liu et al Oncotarget. 2016 Jan 12;7(2):1754-64. do
    Insert
    2xHA-tagged human androgen receptor trancate:DNA-binding domain and Ligand-binding domain (AR Human)
    Tags
    HA
    Expression
    Bacterial and Mammalian
    Mutation
    AR truncate
    Promoter
    CMV
    Available Since
    June 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    171237-plasmid-map-sequence-id-336036

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  39. HisMS2_VLP_RSV

    Plasmid
    #175958
    Purpose
    Generation of MS2 Virus-Like Particles packaged with the sequence for the RSV P and M genes [GenBank: M74568.1]
    Depositor
    Paul Freemont
    Article
    Unruh et al medRxiv 2024.04.17.24305965
    Inserts
    P gene
    M gene
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Sept. 19, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    175958-plasmid-map-sequence-id-357255

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  40. pCas9(D10A)

    Plasmid
    #74495
    Purpose
    Nicking Cas9 derived from pWT Cas9 (Addgene #44250). Ampicillin resistance marker, the tetracycline repressor (TetR), a ColE1 origin of replication and Streptococcus pyogenes Cas9 D10A
    Depositor
    Xiao Wang
    Article
    Standage-Beier et al ACS Synth Biol. 2015 Nov 20;4(11):1217-2
    Insert
    Cas9 D10A
    Use
    Synthetic Biology
    Expression
    Bacterial
    Mutation
    D10A
    Promoter
    pTeto
    Available Since
    May 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    74495-plasmid-map-sequence-id-140495

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  41. pHJL2

    Plasmid
    #199377
    Purpose
    Encodes dual cistronic cytosolic GCaMP6f and mScarlet-I optimized for C. elegans expression
    Depositor
    Hang Lu
    Insert
    GCaMP6f::SL2::mScarlet-I
    Expression
    Bacterial
    Mutation
    no mutations
    Available Since
    April 24, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    199377-plasmid-map-sequence-id-390940

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  43. hCD12

    Plasmid
    #225541
    Purpose
    Expresses human histone deacetylase 6 (HDAC6) catalytic domain 1 and 2 (CD12), also known as hCD12, in Escherichia coli cells.
    Depositor
    David Christianson
    Article
    Hai et al Nat Chem Biol. 2016 Sep;12(9):741-7. doi
    Insert
    Human Histone Deacetylase 6 Catalytic Domain 1 and 2 (HDAC6 Human)
    Tags
    His6-MBP tag with cleavable TEV site
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Sept. 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    225541-plasmid-map-sequence-id-446604

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  50. pET28b-BE3∆UGI

    Plasmid
    #96943
    Purpose
    The plasmid encoding the His6-rAPOBEC1-XTEN-nCas9 protein (BE3∆UGI) was generated by site-directed mutagenesis using pET28b-BE1 (Addgene plasmid #73018).
    Depositor
    Daesik Kim
    Article
    Kim et al Nat Biotechnol. 2017 May;35(5):475-480.
    Insert
    BE3∆UGI
    Tags
    His6 tag
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Sept. 20, 2017
    Availability
    Academic Institutions and Nonprofits only
    96943-plasmid-map-sequence-id-193667

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Showing: 4201 - 4250 of 32656 results