76,289 results

1. pSBbi 41BBL-CD86 mIL-15

   Plasmid

   #183245

   Purpose

   Expression of human 41BBL, CD86, and membrane bound human IL-15 (IL-15-IL-15Ra) in a Sleeping Beauty transposon vector for use in artificial antigen presenting cells

   Depositor

   Alexander McLellan

   Article

   Dobson et al Immunol Cell Biol. 2023 Aug 16. doi: 10.

   Insert

   41BBL, CD86, mbIL-15 (IL-15-IL-15Ra)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pKP332 (Lenti-OSK1)

   Plasmid

   #21627

   Depositor

   Tim Townes

   Article

   Chang et al Stem Cells. 2009 May . 27(5):1042-9.

   Insert

   hOCT4 - 2A - hSOX2 - 2A - hKLF4 (POU5F1, SOX2, KLF4 Porcine teschovirus-1, Human)
   

   Use

   Cre/Lox and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 12, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. mRFP-Rab5

   Plasmid

   #14437

   Purpose

   Mammalian expression of Rab5 fused to mRFP. Used for localization to the early endosome, and other early endosomal vacuoles.

   Depositor

   Ari Helenius

   Article

   Vonderheit et al PLoS Biol. 2005 Jul . 3(7):e233.

   Insert

   Rab 5 (RAB5A Human)
   

   Use

   Tags

   mRFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 23, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-Syn-ChrimsonR-tdT

   Plasmid

   #59171

   Purpose

   AAV-mediated expression of ChrimsonR-tdTomato under the Syn promoter

   Depositor

   Edward Boyden

   Article

   Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

   Has Service

   AAV1, AAV5, and AAV9

   Insert

   ChrimsonR-tdTomato
   

   Use

   AAV

   Tags

   tdTomato

   Expression

   Mammalian

   Mutation

   Promoter

   Syn

   Available since

   Aug. 13, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. Nrxn3b-Nav1.6-MKII-f-hLOV1-TEVcs(ENLYFQ/M)-tTA-VP16

   Plasmid

   #163031

   Purpose

   FLiCRE TF with fast kinetics for neuronal expression

   Depositor

   Alice Ting

   Article

   Kim et al Cell. 2020 Dec 23;183(7):2003-2019.e16.

   Insert

   Nrxn3b-MKII-f-hLOV1-TEVcs-tTA
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Synapsin

   Available since

   March 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. qTAG-C-dTAG-Puro

   Plasmid

   #207727

   Purpose

   Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a dTAG-2A-Puro cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

7. AAVS1-idCas9-vpr

   Plasmid

   #89985

   Purpose

   inducible CRISPR-ON system for controllable gene activation; this plasmid is used to insert dCas9-VPR casette in one allele of AAVS1 locus

   Depositor

   Jie Na

   Article

   Guo et al Protein Cell. 2017 Jan 23. doi: 10.1007/

   Insert

   dCas9-VPR
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE-tight

   Available since

   June 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLenti-DsRed_IRES_EGFP

   Plasmid

   #92194

   Purpose

   Lentiviral overexpression vector to make stable bicistronic cell line for control (EGFP) screen

   Depositor

   Huda Zoghbi

   Article

   Rousseaux et al Elife. 2016 Oct 25;5. pii: e19809. doi:

   Insert

   DsRed-IRES-EGFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. qTAG-N-Puro-dTAG

   Plasmid

   #207694

   Purpose

   Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Puro-2A-dTAG cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

10. TFORF0985

    Plasmid

    #142249

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    KLF4 (KLF4 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. mTurquoise2-N1

    Plasmid

    #54843

    Purpose

    Localization: N1 Cloning Vector, Excitation: 434, Emission: 474

    Depositor

    Michael Davidson, Dorus Gadella

    Article

    Goedhart et al Nat Commun. 2012 Mar 20;3:751. doi: 10.1

    Type

    Empty backbone

    Use

    Tags

    mTurquoise2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 12, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pAAV-pCMV-TM-KA2-CaM-NES-TevN-AsLOV2-TEVseq-tTA

    Plasmid

    #171617

    Purpose

    AAV Soma-targeted Cal-Light with KA2

    Depositor

    Hyungbae Kwon

    Article

    Hyun et al Nat Commun. 2022 Dec 12;13(1):7692. doi:

    Insert

    TM-KA2-CaM-NES-TevN-AsLOV2-TEVseq-tTA
    

    Use

    AAV and Synthetic Biology

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAAV-Syn-FLEX-rc[ChrimsonR-tdTomato]

    Plasmid

    #62723

    Purpose

    AAV-mediated expression of ChrimsonR-tdTomato under the Syn promoter, in floxed/reversed (Cre-dependent) manner. Using bGHpA signal. TdTomato is a codon diversified version.

    Depositor

    Edward Boyden

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Has Service

    AAV1 and AAV5

    Insert

    ChrimsonR-tdTomato
    

    Use

    AAV

    Tags

    tdTomato

    Expression

    Mammalian

    Mutation

    Chrimson K176R mutant

    Promoter

    human synapsin promoter

    Available since

    April 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. GFP-Foxo1

    Plasmid

    #17551

    Depositor

    Domenico Accili

    Article

    Frescas et al J Biol Chem. 2005 May 27. 280(21):20589-

    Insert

    pEGFP-N1 Foxo1 (Foxo1 Mouse)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 8, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLX209-neo-active WRN

    Plasmid

    #125788

    Purpose

    constitutive, ectopic expression of human active WRN

    Depositor

    Francisca Vazquez

    Article

    Chan et al Nature. 2019 Apr 10. pii: 10.1038/s41586

    Insert

    myc-active WRN (WRN Human)
    

    Use

    Tags

    5x myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. qTAG-C-dTAG-Zeo

    Plasmid

    #207738

    Purpose

    Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a dTAG-2A-Zeo cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. PX459v3

    Plasmid

    #178799

    Purpose

    SpCas9 with 2A-Puro, and a golden gate cloning backbone for sgRNA. sgRNA scaffold seqeuence has been modified for increased sgRNA expression (v3).

    Depositor

    Paul Thomas

    Article

    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Cbh (Cas9-2A Puro) and U6 (gRNA)

    Available since

    Aug. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. mRFP-Rab7

    Plasmid

    #14436

    Purpose

    Mammalian expression of Rab7 fused to mRFP. Used for localization to the late endosome, and other early endosomal vacuoles.

    Depositor

    Ari Helenius

    Article

    Vonderheit et al PLoS Biol. 2005 Jul . 3(7):e233.

    Insert

    Rab 7 (RAB7A Canis familialis)
    

    Use

    Tags

    mRFP

    Expression

    Mammalian

    Mutation

    3 silent mutations near the C-terminus

    Promoter

    Available since

    March 23, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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19. PRKAG3 gRNA (BRDN0001145578)

    Plasmid

    #77295

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PRKAG3

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PRKAG3 (PRKAG3 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. PRKAG3 gRNA (BRDN0001147132)

    Plasmid

    #77296

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PRKAG3

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PRKAG3 (PRKAG3 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. PRKAG3 gRNA (BRDN0001146704)

    Plasmid

    #77294

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PRKAG3

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PRKAG3 (PRKAG3 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCAG-NCad-GA

    Plasmid

    #191666

    Purpose

    ddGFP-A-inserted mouse N-cadherin

    Depositor

    Takeharu Nagai

    Article

    Kanadome et al Commun Biol. 2022 Oct 7;5(1):1065. doi:

    Insert

    N-cadherin
    

    Use

    Tags

    HA and ddGFP-A

    Expression

    Mammalian

    Mutation

    Promoter

    Chicken β-actin promoter

    Available since

    Oct. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pCAG-NCad-B1

    Plasmid

    #191667

    Purpose

    ddFP-B1-inserted mouse N-cadherin

    Depositor

    Takeharu Nagai

    Article

    Kanadome et al Commun Biol. 2022 Oct 7;5(1):1065. doi:

    Insert

    N-cadherin
    

    Use

    Tags

    HA and ddFP-B1

    Expression

    Mammalian

    Mutation

    Promoter

    Chicken β-actin promoter

    Available since

    Oct. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. Hygro-Cas9 donor

    Plasmid

    #86883

    Purpose

    Donor vector for genomic targeting of a Tetracycline-inducible Cas9 cassette to the human AAVS1/PPP1R12C locus with hygromycin selection

    Depositor

    Danwei Huangfu

    Article

    Shi et al Cell Stem Cell. 2017 Feb 8. pii: S1934-5

    Insert

    hSpCas9
    

    Use

    CRISPR

    Tags

    3xFlag, Nucleoplasmin NLS, and SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. Lck-Halo_pTwistCMVBetaGlobWPRENeo

    Plasmid

    #221900

    Purpose

    Expresses Halo7 targeted to the plasma membrane

    Depositor

    Janelia Integrative Imaging

    Insert

    Lck-Halo7
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pEGFP-IQGAP1

    Plasmid

    #30112

    Depositor

    David Sacks

    Article

    Ren et al J Biol Chem. 2005 Oct 14. 280(41):34548-

    Insert

    IQGAP1 (IQGAP1 Human)
    

    Use

    Tags

    tandem EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 13, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pCMV5 BRGI-Flag

    Plasmid

    #19143

    Depositor

    Joan Massague

    Article

    Xi et al J Biol Chem. 2008 Jan 11. 283(2):1146-55

    Insert

    SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 (SMARCA4 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 3, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. Puro-Cas9 donor

    Plasmid

    #58409

    Purpose

    Donor vector for genomic targeting of a Tetracycline-inducible Cas9 cassette to the human AAVS1/PPP1R12C locus

    Depositor

    Danwei Huangfu

    Article

    Gonzalez et al Cell Stem Cell. 2014 Jun 11. pii: S1934-

    Insert

    hSpCas9
    

    Use

    CRISPR

    Tags

    3xFlag, Nucleoplasmin NLS, and SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. Flag-FOXM1

    Plasmid

    #153136

    Purpose

    Expression of Flag tagged human FOXM1 in mammalian cells

    Depositor

    Stefan Koch

    Article

    Moparthi et al Differentiation. 2020 Sep - Oct;115:30-3

    Insert

    human FOXM1 (FOXM1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV IE94

    Available since

    Aug. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. TRE-mCherry-p2A-Chrimson-TS

    Plasmid

    #158702

    Purpose

    Reporter construct

    Depositor

    Alice Ting

    Article

    Sanchez et al Proc Natl Acad Sci U S A. 2020 Dec 15. p

    Insert

    TRE-mcherry-p2A-Chrimson-TS
    

    Use

    Synthetic Biology

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pEF6-Acly

    Plasmid

    #70765

    Purpose

    Expresses mouse ATP-citrate lyase in mammalian cells

    Depositor

    Kathryn Wellen

    Article

    Lee et al Cell Metab. 2014 Aug 5;20(2):306-19. doi

    Insert

    ATP Citrate Lyase (Acly Mouse)
    

    Use

    Tags

    myc-epitope

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    Nov. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. qTAG-N-Zeo-dTAG

    Plasmid

    #207705

    Purpose

    Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Zeo-2A-dTAG cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

33. GLUT1-eGFP/pcDNA-DEST47

    Plasmid

    #18729

    Depositor

    Wolf Frommer

    Article

    Takanaga et al Biochim Biophys Acta. 2008 Apr . 1778(4)

    Insert

    pcDNA-DEST47 /hGLUT1-eGFP
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    deleted TAA

    Promoter

    Available since

    July 3, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. TFORF2946

    Plasmid

    #144422

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    MYOD1 (MYOD1 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Sept. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pTRIPZ shTREX1

    Plasmid

    #127700

    Purpose

    Doxycyclin inducible shRNA knockdown of both human and mouse TREX1 gene

    Depositor

    Sandra Demaria

    Article

    Vanpouille-Box et al Nat Commun. 2017 Jun 9;8:15618. doi: 10.

    Insert

    shTREX1 (Trex1 Mouse, Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. MSCV-pU6-(BbsI)-CcdB-(BbsI)-Pgk-Puro-T2A-BFP

    Plasmid

    #86457

    Purpose

    Mouse stem cell retroviral vector including a puromycin resistance and BFP gene. sgRNA targets can be cloned in between the BbsI sites

    Depositor

    Ralf Kuehn

    Article

    Chu et al Proc Natl Acad Sci U S A. 2016 Nov 1;113

    Insert

    BFP
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Pgk

    Available since

    Feb. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pEv-Fat4-citrine

    Plasmid

    #112854

    Purpose

    Constitutive expression of human fat4 protein fused to citrine fluorescent protein

    Depositor

    David Sprinzak

    Article

    Loza et al Elife. 2017 Aug 18;6. pii: 24820. doi: 1

    Insert

    FAT4 (FAT4 Human)
    

    Use

    Tags

    Citrine

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. GfaABC1D HRP-TM

    Plasmid

    #231499

    Purpose

    Expressing HRP on the cell surface of astrocytes

    Depositor

    Baljit Khakh

    Article

    Wu et al Neuron. 2025 Jun 5:S0896-6273(25)00366-6

    Insert

    HRP
    

    Use

    AAV

    Tags

    Igk, HA, Myc, PDGFR-beta transmembrane domain

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pcDNA5-T/O-Ds1-mCherry

    Plasmid

    #112855

    Purpose

    Dox inducible expression in mammalian cells of Ds1 protein fused to mCherry fluorescent protein

    Depositor

    David Sprinzak

    Article

    Loza et al Elife. 2017 Aug 18;6. pii: 24820. doi: 1

    Insert

    DCHS1 (DCHS1 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    doxycycline inducible promoter

    Available since

    Aug. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pSBbi 41BBL CD86 HLA-A201

    Plasmid

    #183248

    Purpose

    Expression of human 41BBL, CD86 and HLA-A201 in a Sleeping Beauty transposon vector for use in artificial antigen presenting cells

    Depositor

    Alexander McLellan

    Article

    Dobson et al Immunol Cell Biol. 2023 Aug 16. doi: 10.

    Inserts

    CD86, 41BBL
    HLA-A2 (HLA-A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge