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78,649 results

Showing: 6571 - 6600 of 78649 results

  4. pcDNA_mIGg2a_Anti-HA [12CA5] v2

    Plasmid
    #236295
    Purpose
    Mammalian vector expressing Anti-HA [12CA5] variable region fused to mouse IgG2a heavy chain; to be used with pcDNA_mK_Anti-HA [12CA5] v2 light chain (Plasmid 236296) to make the antibody.
    Depositor
    Melina Fan, Addgene Research Program
    Insert
    Anti-HA [12CA5] heavy chain variable region fused to mouse IgG2a heavy chain
    Tags
    Mouse IgG2a Fc
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Aug. 18, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    236295-plasmid-map-sequence-id-467832

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  5. pcDNA_mK_Anti-HA [12CA5] v2

    Plasmid
    #236296
    Purpose
    Mammalian vector expressing Anti-HA [12CA5] variable region fused to mouse Kappa light chain; to be used with pcDNA_mIGg2a_Anti-HA [12CA5] v2 heavy chain (Plasmid 236295) to make the antibody.
    Depositor
    Melina Fan, Addgene Research Program
    Insert
    Anti-HA [12CA5] light chain variable region fused to mouse kappa light chain
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Aug. 18, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    236296-plasmid-map-sequence-id-467834

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  7. LD-B

    Plasmid
    #209861
    Purpose
    Dimerization dependent fluorescent protein B anchored to cytosolic face of the lipid droplet membrane with hairpin domain of human GPAT4
    Depositor
    Sarah Cohen
    Article
    Miner et al Contact (Thousand Oaks). 2024 Feb 4;7:25
    Insert
    ddGFP B
    Tags
    Hairpin domain of GPAT4 MNFQYISLRLTVLWGLGVLIRYCFL…
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Nov. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    209861-plasmid-map-sequence-id-415472

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  11. TFORF2883

    Plasmid
    #144255
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    BHLHA9 (BHLHA9 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    May 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    144255-plasmid-map-sequence-id-392721

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  19. pC0055-CMV-dPspCas13b-GS-ADAR2DD(E488Q/T375G)-delta-984-1090

    Plasmid
    #103871
    Purpose
    dPspCas13b-ADAR2DD(E488Q/T375G) fusion that can be used to selectively edit adenosine to inosine in RNA when used in conjuction with a guide RNA. Cas13b is truncated to allow for expression by AAV
    Depositor
    Feng Zhang
    Article
    Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi
    Inserts
    PspCas13b
    huADAR2DD (ADARB1 Human)
    Use
    CRISPR
    Tags
    GS and HIV NES
    Expression
    Mammalian
    Mutation
    Changed histidne 133 to alanine and histidine 105…
    Available Since
    Nov. 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    103871-plasmid-map-sequence-id-259670

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  21. pICE-RNaseHI-WT-NLS-mCherry

    Plasmid
    #60365
    Purpose
    Plasmid for expression of bacterial RNase HI tagged with NLS-mCherry that can be used to degrade R-loops. Confers resistance to Puromycin. Expression is inducible in T-REx cells.
    Depositor
    Sébastien Britton, Patrick Calsou
    Article
    Britton et al Nucleic Acids Res. 2014 Oct 1;42(14):904
    Insert
    RNase HI
    Tags
    NLS from SV40 T antigen and mCherry
    Expression
    Mammalian
    Promoter
    CMV-tet
    Available Since
    Jan. 8, 2015
    Availability
    Academic Institutions and Nonprofits only
    60365-plasmid-map-sequence-id-256737

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  26. pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA

    Plasmid
    #20298
    Purpose
    Cre-activated AAV expression of humanized ChR2 with H134R mutation fused to EYFP for optogenetic activation
    Depositor
    Karl Deisseroth
    Has Service
    AAV PHP.eB, AAV Retrograde, AAV1, AAV5, and AAV9
    Insert
    channelrhodopsin-2
    Use
    AAV
    Tags
    EYFP
    Expression
    Mammalian
    Mutation
    humanized ChR2 gene with histidine 134 changed to…
    Promoter
    EF1alpha
    Available Since
    May 13, 2009
    Availability
    Academic Institutions and Nonprofits only
    20298-plasmid-map-sequence-id-191904

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  27. pScRad52-SpCas9 (pXK-1646)

    Plasmid
    #208823
    Purpose
    CRISPR/Rad52-Cas9 expression vector for Animal gene editing, harboring the ScRad52-SpCas9 and a VEGFA.gRNA expression cassette. Key Construct: hU6-VEGFa.gRNA-CMV-ScRad52-SpCas9.
    Depositor
    Kun Xu
    Article
    Shao et al Int J Biochem Cell Biol. 2017 Nov;92:43-
    Type
    Empty backbone
    Use
    CRISPR; Cas9
    Tags
    Rad52
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Dec. 19, 2023
    Availability
    Academic Institutions and Nonprofits only
    208823-plasmid-map-sequence-id-417236

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Showing: 6571 - 6600 of 78649 results