81,074 results

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pSAM2_mCherry_Gata4

Plasmid

#72690

Purpose

Doxycyline regulated expression of Gata4 in mammalian cells

Depositor

Timothy Kamp

Article

Lalit et al Cell Stem Cell. 2016 Mar 3;18(3):354-67.

Insert

Gata 4 (Gata4 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV-Doxycycline

Available Since

Aug. 28, 2017

Availability

Academic Institutions and Nonprofits only

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pCAGImC_V5-CSNK1A1

Plasmid

#92014

Purpose

Expresses V5-CSNK1A1, includes IRES-mCherry

Depositor

Joshua Mendell

Article

Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

Insert

CSNK1A1 (CSNK1A1 Human)

Tags

V5

Expression

Mammalian

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pcU6_3 sgRNA

Plasmid

#92359

Purpose

Chicken-specific U6 sgRNA expression mini-vector, harbouring chick U6_3 pol III promoter.

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Development. 2018 Feb 23;145(4). pii: de

Insert

chick U6.3 promoter and gRNA cloning cassette

Use

CRISPR

Expression

Mammalian

Promoter

chick U6.3

Available Since

Aug. 24, 2017

Availability

Academic Institutions and Nonprofits only

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pX330a dCas9-VP64

Plasmid

#92363

Purpose

Modified CAG promoter-containing vector for ubiquitous expression of catalytically inactive Cas9 fused to VP64 transcriptional activation domain. For targeted gene activation in chicken embryos.

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Development. 2018 Feb 23;145(4). pii: de

Insert

dCas9-VP64

Use

CRISPR

Expression

Mammalian

Promoter

CAG

Available Since

Aug. 24, 2017

Availability

Academic Institutions and Nonprofits only

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pLenti-PRSX8ChR2 (H134R)eYFP

Plasmid

#89539

Purpose

Expresses ChR2 (H134R) -eYFP in Phox2 (including catecholamine) neurons

Depositor

Ruth Stornetta

Article

Holloway et al Eur J Neurosci. 2015 Sep;42(6):2271-82.

Insert

ChR2 (H134R)

Use

Lentiviral

Tags

EYFP

Expression

Mammalian

Available Since

Aug. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Flag-humanTRAF3 (1096)

Plasmid

#66928

Purpose

Mammalian Expression of TRAF3

Depositor

Michael Karin

Insert

TRAF3 (TRAF3 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 7, 2017

Availability

Academic Institutions and Nonprofits only

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ITPKA-mScarlet-I

Plasmid

#98829

Purpose

In vivo visualization of actin cytoskeleton (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

Insert

ITPKA

Tags

mScarlet-I

Expression

Mammalian

Promoter

CMV

Available Since

July 28, 2017

Availability

Academic Institutions and Nonprofits only

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Lenti-sgp53/Cre

Plasmid

#89646

Purpose

Expresses a p53-targeting gRNA and Cre-recombinase

Depositor

Monte Winslow

Article

Rogers et al Nat Methods. 2017 May 22. doi: 10.1038/n

Insert

gRNA targeting p53 (Trp53 Mouse)

Use

Cre/Lox and Lentiviral

Expression

Mammalian

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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pGP-CMV-GCaMP6-150

Plasmid

#91777

Purpose

Mammalian expression of low affinity calcium-sensing fluorescent protein

Depositor

Douglas Kim, GENIE Project

Article

de Juan-Sanz et al Neuron. 2017 Jan 30. pii: S0896-6273(17)

Insert

GCaMP6-150

Tags

6xHis, T7 epitope, and Xpress tag

Expression

Mammalian

Promoter

CMV

Available Since

June 27, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-NES-CRY2PHR-TevC

Plasmid

#89877

Purpose

expresses NES-CRY2PHR-TevC of BLITz system

Depositor

Hyungbae Kwon

Article

Lee et al Nat Methods. 2017 May;14(5):495-503. doi

Insert

NES-CRY2PHR-TevC

Expression

Mammalian

Promoter

CMV

Available Since

May 26, 2017

Availability

Academic Institutions and Nonprofits only

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AICSDP-24:MYH10-mEGFP

Plasmid

#87428

Purpose

Homology arms and linker-mEGFP sequence for N-terminus tagging of human MYH10

Depositor

Allen Institute for Cell Science

Article

Roberts et al Mol Biol Cell. 2017 Oct 15;28(21):2854-2

Insert

MYH10 Homology Arms with mEGFP-linker (MYH10 Human)

Use

CRISPR; Donor template

Tags

mEGFP-linker

Expression

Mammalian

Mutation

homology arms contain point mutations to disrupt …

Available Since

May 2, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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pHR-FKBP:mCherry-Rab11a

Plasmid

#72902

Purpose

Recycling endosome-targeted mCherry-tagged recruiter construct

Depositor

Ivan Yudushkin

Article

Ebner et al J Cell Biol. 2017 Jan 31. pii: jcb.20161

Insert

Rab11a (RAB11A Human)

Use

Lentiviral

Tags

FKBP and mCherry

Expression

Mammalian

Mutation

fragment a.a. 2-216

Available Since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

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pHR-FKBP:mCherry-Rab7a

Plasmid

#72903

Purpose

Late endosome-targeted mCherry-tagged recruiter construct

Depositor

Ivan Yudushkin

Article

Ebner et al J Cell Biol. 2017 Jan 31. pii: jcb.20161

Insert

Rab7a (RAB7A Human)

Use

Lentiviral

Tags

FKBP, mCherry

Expression

Mammalian

Available Since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

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LOTUS-V/pcDNA3

Plasmid

#87127

Purpose

Bioluminescent and ratiometric voltage indicator

Depositor

Takeharu Nagai

Article

Inagaki et al Sci Rep. 2017 Feb 13;7:42398. doi: 10.10

Insert

LOTUS-V

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 28, 2017

Availability

Academic Institutions and Nonprofits only

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pMXs3-TIAM1

Plasmid

#86143

Purpose

Retroviral vector expressing TIAM1-IRES-RFP

Depositor

David Sabatini

Article

Wang et al Cell. 2017 Feb 1. pii: S0092-8674(17)300

Insert

TIAM1 (TIAM1 Human)

Use

Retroviral

Expression

Mammalian

Promoter

LTR

Available Since

Feb. 22, 2017

Availability

Academic Institutions and Nonprofits only

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ER50-SpCas9-ER50

Plasmid

#85448

Purpose

Expresses SpCas9 fused to ER50 domain on both N- and C-termini in mammalian cells

Depositor

Amit Choudhary

Article

Maji et al Nat Chem Biol. 2017 Jan;13(1):9-11. doi:

Insert

ER50-SpCas9-ER50

Use

AAV

Tags

destabilized domain of estrogen receptor ligand b…

Expression

Mammalian

Promoter

Cbh

Available Since

Feb. 9, 2017

Availability

Academic Institutions and Nonprofits only

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SHP2(WT)@pLKO-Trex-SBP

Plasmid

#85457

Purpose

Gene expression

Depositor

Darrin Stuart

Article

Chen et al Nature. 2016 Jul 7;535(7610):148-52.

Insert

SHP2 (PTPN11 Human)

Use

Lentiviral

Tags

SBP Tag

Expression

Mammalian

Promoter

RSV

Available Since

Feb. 6, 2017

Availability

Academic Institutions and Nonprofits only

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pBA409

Plasmid

#85971

Purpose

EF1a-sfGFP-Ubc-Neo

Depositor

Jonathan Weissman

Article

Adamson et al Cell. 2016 Dec 15;167(7):1867-1882.e21.

Inserts

sfGFP
Neo

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pTRIPZ (M)-H2A-HT

Plasmid

#82517

Purpose

Lentivirus vector. Expresses H2A-Halotag fusion proteins in mammalian cells.

Depositor

Xiaojun Ren

Insert

Histone H2A

Use

Lentiviral

Tags

HaloTag

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pcsII-LMP7-flag

Plasmid

#86773

Purpose

C-terminal flag-tagged protein expression in mammalian cells, lentiviral vector

Depositor

Do-Hyung Kim

Article

Yun et al Mol Cell. 2016 Feb 18;61(4):625-39. doi:

Insert

immunoproteasome beta subunit 5 (LMP7) (PSMB8 Human)

Use

Lentiviral

Tags

flag

Expression

Mammalian

Promoter

EF-1a

Available Since

Jan. 26, 2017

Availability

Academic Institutions and Nonprofits only

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pTRE-Tight-BI-Gl NORM-LacZA-TER-LacZB

Plasmid

#86194

Purpose

beta Globin NMD construct with and without PTC under Bi-directional TET on promoter

Depositor

Robert Singer

Article

Trcek et al Genes Dev. 2013 Mar 1;27(5):541-51. doi:

Insert

beta Globin (HBB, Hbb Human, Mouse)

Use

Tet on

Expression

Mammalian

Mutation

39TER in beta Globin in MCS II

Promoter

Tet on

Available Since

Jan. 11, 2017

Availability

Academic Institutions and Nonprofits only

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ITSN-mcherry-sspB

Plasmid

#85220

Purpose

Role of Cdc-42 selective GEF domain from ITSN in immune cell migration

Depositor

Narasimhan Gautam

Article

O'Neill et al Mol Biol Cell. 2016 May 1;27(9):1442-50.

Inserts

ITSN1(1225-1575) (ITSN1 Human)
mcherry
sspB

Tags

NA

Expression

Mammalian

Mutation

R73Q

Promoter

CMV

Available Since

Jan. 5, 2017

Availability

Academic Institutions and Nonprofits only

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pSLIK DN RhoA

Plasmid

#84646

Purpose

Lentiviral expression of doxycycline-inducible dominant negative RhoA and co-expression of Venus

Depositor

Sanjay Kumar

Article

MacKay et al Integr Biol (Camb). 2014 Sep;6(9):885-94

Insert

RhoA (RHOA Human)

Use

Lentiviral

Tags

Myc

Expression

Mammalian

Mutation

Dominant Negative

Available Since

Dec. 9, 2016

Availability

Academic Institutions and Nonprofits only
pGH020_sgRNA_G418-GFP

Plasmid

#85405

Purpose

hu6 driven sgRNA vector with G418 and GFP selectable markers

Depositor

Michael Bassik

Article

Hess et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

Insert

G418 resistance and GFP

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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gRNA Cloning Vector Bbs I ver. 2

Plasmid

#85586

Purpose

An empty sgRNA expression vector. sgRNA sequence can be cloned into its Bbs I site. Its gRNA scaffold has efficient ver. 2 structure.

Depositor

Hodaka Fujii

Article

Fujita et al Sci Rep. 2016 Jul 28;6:30485. doi: 10.10

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Dec. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pTRIPZ (M)-HT-Cbx4

Plasmid

#82513

Purpose

Lentivirus vector. Expresses Halotag-Cbx4 fusion proteins in mammalian cells.

Depositor

Xiaojun Ren

Insert

Chromobox Homolog 4 (Cbx4 Mouse)

Use

Lentiviral

Tags

HaloTag

Expression

Mammalian

Promoter

cmv

Available Since

Dec. 1, 2016

Availability

Academic Institutions and Nonprofits only

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pSLQ2814 pPB: CAG-Puro-WPRE PGK-VPR-tagBFP-SpdCas9

Plasmid

#84247

Purpose

Expresses direct fusion VPR-Sp dCas9

Depositor

Stanley Qi

Article

Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

Inserts

VPR-tagBFP-Sp dCas9
Puro

Use

CRISPR and Synthetic Biology; Piggybac

Tags

2xNLS (SV40), HA tag, VPR, and tagBFP

Expression

Mammalian

Promoter

CAG and PGK

Available Since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1A eIF4A3

Plasmid

#83035

Purpose

mammalian expression of eIF4A3

Depositor

Gideon Dreyfuss

Article

Chan et al RNA. 2004 Feb;10(2):200-9.

Insert

eIF4A3 (EIF4A3 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 27, 2016

Availability

Academic Institutions and Nonprofits only
pCAGGS-4EBP1 F113A

Plasmid

#81122

Purpose

expresses a constitutively active mutant of 4E-BP1 under the CAG promoter

Depositor

Angelique Bordey

Article

Hartman et al Cell Rep. 2013 Oct 31;5(2):433-44. doi:

Insert

4EBP1 F113A (Eif4ebp1 Mouse)

Expression

Mammalian

Mutation

F113A mutation

Promoter

CAG

Available Since

Sept. 21, 2016

Availability

Academic Institutions and Nonprofits only

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FSW-HRP-V5-LRRTM1

Plasmid

#82536

Purpose

Lentiviral vector for expressing HRP-LRRTM1 in neurons

Depositor

Alice Ting

Article

Loh et al Cell. 2016 Aug 25;166(5):1295-1307.e21.

Insert

LRRTM1 (LRRTM1 Human)

Use

Lentiviral

Tags

HRP and V5

Expression

Mammalian

Promoter

Human Synapsin

Available Since

Sept. 21, 2016

Availability

Academic Institutions and Nonprofits only

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81,074 results