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80,556 results

Showing: 1761 - 1800 of 80556 results

  3. dCas9-miniTurbo

    Plasmid
    #219822
    Purpose
    EF1α-driven expression of dCas9 fused to miniTurbo with GFP (Adapted from plasmid #153209)
    Depositor
    Philipp Maass
    Insert
    dCas9
    Use
    CRISPR
    Tags
    miniTurbo
    Expression
    Mammalian
    Mutation
    D10A/H841A
    Promoter
    EF1a
    Available Since
    July 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    219822-plasmid-map-sequence-id-482202

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  5. AAV-sCAG-mBaoJin-oPRE

    Plasmid
    #218684
    Purpose
    AAV vector plasmid expressing mBaoJin under a short variant of the CAG (sCAG) promoter. It also contains an optimised and truncated version of WPRE as a regulatory element.
    Depositor
    Bart Nieuwenhuis
    Insert
    mBaoJin
    Use
    AAV
    Expression
    Mammalian
    Promoter
    Short variant of the CAG promoter
    Available Since
    July 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    218684-plasmid-map-sequence-id-438234

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  8. pBrain-GFP-TACC3KDP-shTACC3

    Plasmid
    #59356
    Purpose
    Knocks down endogenous TACC3 and re-expresses knockdown-proof (RNAi-resistant) GFP-tagged human TACC3.
    Depositor
    Stephen Royle
    Article
    Booth et al EMBO J. 2011 Mar 2;30(5):906-19. doi: 10
    Insert
    Transforming Acidic Coiled Coil protein 3 (TACC3 Human)
    Use
    RNAi
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    Silent mutations in amino acids 30-33 to confer s…
    Promoter
    CMV
    Available Since
    Dec. 5, 2014
    Availability
    Academic Institutions and Nonprofits only
    59356-plasmid-map-sequence-id-94885

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  9. 24xMoonTag-kif18b-STOP-24xSunTag-linker-24xPP7 (3’UTR translation reporter)

    Plasmid
    #128605
    Purpose
    Expresses 24xgp41 peptide array (MoonTag peptide array), followed by kif18b gene, STOP codon, 24xgcn4 peptide array (SunTag peptide array), linker and 24 repeats of PP7 hairpins.
    Depositor
    Marvin Tanenbaum
    Article
    Boersma et al Cell. 2019 Jun 5. pii: S0092-8674(19)304
    Inserts
    24xMoonTag
    kinesin family member 18b (KIF18B Human)
    24xSunTag
    Expression
    Mammalian
    Promoter
    Pcmv
    Available Since
    Jan. 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    128605-plasmid-map-sequence-id-318004

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  18. MLM3636

    Plasmid
    #43860
    Purpose
    guide RNA (gRNA) expression vector used to create a gRNA to a specific sequence, uses U6 promoter
    Depositor
    Keith Joung
    Insert
    None
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    March 8, 2013
    Availability
    Academic Institutions and Nonprofits only
    43860-plasmid-map-sequence-id-194368

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  19. EGFR-LgBiT, BiBiT-Ready Vector

    Plasmid
    #238755
    Purpose
    Express EGFR-LgBiT Fusion Protein in Mammalian Cells under a CMV promoter
    Depositor
    Promega Corporation
    Has Service
    DNA
    Insert
    EGFR (EGFR Human)
    Tags
    LgBiT
    Expression
    Mammalian
    Mutation
    None
    Promoter
    CMV
    Available Since
    Sept. 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    238755-plasmid-map-sequence-id-479808

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  25. lenti sgRNA(MS2)_zeo backbone

    Plasmid
    #61427
    Purpose
    3rd generation lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2 and EF1a-zeo resistance marker. Contains BsmBI sites for insertion of spacer sequences.
    Depositor
    Feng Zhang
    Article
    Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    U6 and EF1A
    Available Since
    Dec. 15, 2014
    Availability
    Academic Institutions and Nonprofits only
    61427-plasmid-map-sequence-id-322387

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  32. pSLQ2821 pPB: CAG-GID1-VPR-IRES-Puro-WPRE PGK-GAI-tagBFP-SpdCas9-ABI

    Plasmid
    #84257
    Purpose
    Expresses Sp dCas9 and GA-inducible VPR for OR gate/diametric regulation
    Depositor
    Stanley Qi
    Article
    Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n
    Inserts
    GAI-tagBFP-Sp dCas9-ABI
    GID1-VPR
    Use
    CRISPR and Synthetic Biology; Piggybac
    Tags
    2xNLS (SV40), ABI, GAI, GID1, HA tag, IRES-Puro, …
    Expression
    Mammalian
    Promoter
    CAG and PGK
    Available Since
    Feb. 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    84257-plasmid-map-sequence-id-160891

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  38. TRUPATH Triple (bidirectional) GasS

    Plasmid
    #196055
    Purpose
    Encodes a G alpha subunit (GNAS2) with RLuc8, a G gamma subunit (GNG9) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins
    Depositor
    Justin English
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Inserts
    GAlphasS-RLuc8
    GGamma9-GFP2
    GBeta3 (GNB3 Human)
    Use
    Luciferase
    Tags
    GFP2 and GSAG linker
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available Since
    March 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    196055-plasmid-map-sequence-id-385866

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  39. VHL-NanoLuc Vector

    Plasmid
    #238827
    Purpose
    Express VHL-NanoLuc Fusion Protein in Mammalian Cells under a CMV promoter
    Depositor
    Promega Corporation
    Has Service
    DNA
    Insert
    VHL (VHL Human)
    Tags
    NanoLuc (R)
    Expression
    Mammalian
    Mutation
    None
    Promoter
    CMV
    Available Since
    Sept. 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    238827-plasmid-map-sequence-id-481548

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Showing: 1761 - 1800 of 80556 results